Abstract：
:Nuclear protein and DNA content of HeLa cells was determined as a function of time following hyperthermia by staining isolated nuclei with two fluorescent dyes: fluorescein isothiocyanate (FITC) for protein content and propidium iodide (PI) for DNA content. Bivariate FITC and PI histograms were obtained by flow cytometry. Univariate flow cytometric analysis was shown to be inadequate for this study, because some of the nuclear protein changes were due to cell cycle redistribution. Posthyperthermia cell kinetics could be divided into two distinct phases: an early phase characterized by the removal of heat-induced excess nuclear proteins with little or no cell progression through the cell cycle; and a late phase characterized by a redistribution of cells in the cell cycle resulting in an accumulation of cells in G2. The duration of these phases was dependent upon the hyperthermia dose. In the early phase, the rate of removal of excess nuclear protein was found to vary with heating time and temperature for time-temperature combinations which resulted in the same amount of excess nuclear protein. In the late phase, the cells blocked in G2 did not reduce their nuclear protein levels back to control values.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Roti Roti JL,Uygur N,Higashikubo Rsubject
Has Abstractpub_date
1986-08-01 00:00:00pages
250-61issue
2eissn
0033-7587issn
1938-5404journal_volume
107pub_type
杂志文章abstract：:The investigation of fragment length distributions of plasmid DNA gives insight into the influence of localized energy distribution on the induction of DNA damage, particularly the clustering of double-strand breaks. We present an approach that determines the fragment length distributions of plasmid DNA after heavy-io...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1028.1
更新日期：2008-06-01 00:00:00
abstract：:Exposure of cells to heat induces thermotolerance, a transient resistance to subsequent heat challenges. It has been shown that thermotolerance is correlated in time with the enhanced synthesis of heat shock proteins. In this study, the association of induced heat shock proteins with various cellular fractions was inv...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
abstract：:To determine whether comparative proteomics could detect differential protein expression after lung irradiation in two mouse strains with different radiation responses, lung proteins were subjected to two-dimensional orthogonal liquid-phase separations, with chromatofocusing in the first dimension and nonporous silica...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1173.1
更新日期：2008-04-01 00:00:00
abstract：:The basic 30-nm chromatin fiber in the mammalian cell consists of an unknown (possibly helical) arrangement of nucleosomes, with about 1.2 kb of DNA per 10-nm length of fiber. Track-structure considerations suggest that interactions of single delta rays or high-LET particles with the chromatin fiber might result in th...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：:The correlation between the protein content of nuclei and heat-induced cell killing was determined for HeLa cells with various levels of thermotolerance. Thermotolerance was induced by heating cells at 45 degrees C for 15 or 30 min and then incubating them at 37 degrees C for 5 or 24 h. This procedure resulted in four...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:We have studied the effect on sister chromatid exchange (SCE) induction in 9L rat brain tumor cells caused by combination treatment with BCNU and X rays. Over the dose and concentration ranges used in these experiments, BCNU induced relatively large numbers of SCEs, while X rays induced few SCEs. When cells were X irr...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:The effectiveness of 5-fluorouracil (5-FU), FT-207 and FT-207 + uracil in combination with two repetitions of 43 degrees C hyperthermia in the treatment of the Meth-A-Fibrosarcoma and Sarcoma-180 was examined in vivo in BALB/c mice. The antitumor effect was evaluated in terms of inhibition of tumor growth by measuring...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
abstract：:Abstract Double-strand breaks (DSBs) can be generated in the DNA of mammalian cells when heat-labile sites induced by ionizing radiation within a clustered DNA damage site are thermally transformed to single-strand breaks (SSBs) and combine with other SSBs or heat-labile sites in the opposite DNA strand. When this the...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1745.1
更新日期：2009-08-01 00:00:00
abstract：:Mathematical analysis of cell survival allows parameter estimation for radiobiological models and selection of an appropriate model. To our knowledge, no rigorous comparisons on the accuracy of various methods used for such analysis have been performed. In this study we compared three methods: 1. maximization of binom...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14195.1
更新日期：2016-03-01 00:00:00
abstract：:In pathogen-free mice, but not standard conventionally housed laboratory rodents, two distinctly different modes of early radiation lethality can be identified by modifying the irradiation technique (total-body versus abdominal irradiation) or by therapeutic intervention such as rescue of total-body-irradiated mice wi...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:The present study demonstrates the usefulness of immunochemical assays for quantitating modified bases in oxidized and X-irradiated DNA. Escherichia coli, phi X174 RF I, PM2, and M13 DNA containing thymine glycols introduced by OsO4 oxidation were used as antigens in a direct enzyme-linked immunosorbent assay (ELISA)....
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：:A biphasic curve was observed when surviving allogeneic lytic activity was plotted as a function of irradiation delivered before sensitization. Flow cytometry analysis demonstrated that the number of cells was reduced in response to increasing dose and that subset precursors Lyt 1+2+ were proportionally more radiosens...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:There has been ongoing debate and discussion concerning whether the funding source influenced the outcome of research on human health effects from exposure to radiofrequency fields (RF, electromagnetic waves that carry energy as they propagate in air and dense media). In a study of 225 publications, in which we sought...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR15364.1
更新日期：2019-10-01 00:00:00
abstract：:The current status of particle radiotherapy from a historical perspective is presented. This is done with a personal view and contains personal references and memories during the development of particle radiotherapy. The particles covered are fast neutrons, neutron capture therapy, protons, helium ions, pions and heav...
journal_title：Radiation research
pub_type： 历史文章,杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:Radiation-induced apoptosis in lymphocyte-derived cell lines is characterized by endonucleolytic cleavage of cellular DNA within hours after radiation exposure. We have studied this phenomenon qualitatively (DNA gel electrophoresis) and quantitatively (diphenylamine reagent assay) in murine EL4 T-lymphoma cells expose...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:Vascular injury after radiation exposure contributes to multiple types of tissue injury through a cascade of events. Some of the earliest consequences of radiation damage include increased vascular permeability and promotion of inflammation, which is partially manifested by increased leukocyte-endothelial (L/E) intera...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14896.1
更新日期：2018-07-01 00:00:00
abstract：:1-(2-Fluoro-4-iodo-2,4-dideoxy-beta-L-xylopyranosyl)-2-nitroimidazole (FIAZP) has been synthesized and labeled with radioiodine (125I). Radioiodinated FIAZP is one of a series of sugar-coupled 2-nitroimidazoles developed in our laboratory as probes for noninvasive scintigraphic assessment of tumor hypoxia. An in vivo ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:Keratinocyte growth factor (FGF7, also known as KGF) ameliorates the radiation response of mouse oral mucosa and other epithelial tissues. However, the precise mechanisms remain unclear. The aim of the present study was to investigate the effect of FGF7 on the survival and colony size of normal human epidermal keratin...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)156[0761:eokgfo]2.0.co;2
更新日期：2001-12-01 00:00:00
abstract：:Chinese hamster ovary cells in suspension cultures were heated for various times at 41.5, 43.5, and 45.5 degrees C, and quantitative determinations of microblebbing and macroblebbing of the cell membrane were performed for cells maintained at 4, 25, and 37 degrees C after hyperthermia. The percentage of cells with ble...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:Domestic radon risk estimates are typically based either on data for uranium miners or on data derived from A-bomb survivors; comparison of domestic radon risk estimates derived from these two disparate sources represents an important test of their reliability. There is currently a significant discrepancy of about a f...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:The linear no-threshold extrapolation from a dose-response relationship for ionizing radiation derived at higher doses to doses for which regulatory standards are proposed is being challenged by some scientists and defended by others. It appears that the risks associated with exposures to doses of interest are below t...
journal_title：Radiation research
pub_type：
doi：
更新日期：1998-12-01 00:00:00
abstract：:Cancer mortality in relation to radiation dose was evaluated among 4153 women treated with intrauterine radium (226Ra) capsules for benign gynecologic bleeding disorders between 1925 and 1965. Average follow up was 26.5 years (maximum = 59.9 years). Overall, 2763 deaths were observed versus 2687 expected based on U.S....
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-09-01 00:00:00
abstract：:Studies were conducted to determine the effects of BeSO4 or X rays, alone and in combination, on cell cycle kinetics, cell killing, and the production of chromosome aberrations in Chinese hamster ovary (CHO) cells. The concentration of BeSO4 required to kill 50% of CHO cells exposed to BeSO4 for 20 h was determined to...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:The effects of multiple-dose gamma irradiation on the shape of survival curves were studied with mouse C3H 10T1/2 cells maintained in contact-inhibited plateau phase. The dose-fractionation intervals included 3, 6, and 24 h. Following three fractionated doses (5 Gy per dose) of exposures, cells responded to further ir...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1986-06-01 00:00:00
abstract：:The molecular structure of mutants induced in human lymphoblast cells by 500 cGy X rays in the presence of the radioprotector cysteamine (25 mM) has been compared with that induced by an equally mutagenic treatment of 150 cGy X rays alone. Sets of mutants at the hypoxanthine-guanine phosphoribosyl transferase locus we...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：:Takagaki, M., Ono, K., Masunaga, S-I., Kinashi, Y., Oda, Y., Miyatake, S-I., Hashimoto, N., Powell, W., Sood, A. and Spielvogel, B. F. Boronated Dipeptide Borotrimethylglycylphenylalanine as a Potential Boron Carrier in Boron Neutron Capture Therapy for Malignant Brain Tumors. Radiat. Res. 156, 118-122 (2001).A borona...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2001)156[0118:bdbaap]2.0.co;2
更新日期：2001-07-01 00:00:00
abstract：:The nature of the primary biochemical defect in the human radiosensitive and cancer-prone syndrome, ataxia telangiectasia (AT), has remained obscure despite many efforts to elucidate it. In this study, AT complementation group D cells and a nearly isogenic corrected AT-hamster hybrid derivative have been analyzed for ...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:The present paper describes experiments conducted over the last several years which have focused primarily on the development of mammary tumors in BALB/c mice after neutron and gamma irradiation. Time-dose relationships for induction of mammary and lung tumors following irradiation with fission-spectrum neutrons and 1...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:Cytoglobin is a recently identified vertebrate globin whose functions include scavenging reactive oxygen and nitrosative species. In tumor cells, CYGB may function as a tumor suppressor gene. Here we show that knockdown of cytoglobin expression can sensitize human glioma cells to oxidative stress induced by chemical i...
journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr2517.1
更新日期：2011-08-01 00:00:00
abstract：:The relationship between intracellular and extracellular pH was investigated in a murine tumor and normal tissue, prior to and following glucose injection. Isotransplants of the murine tumor FSa-II in the dorsum of the hind foot and leg muscle, were investigated in nonanesthetized mice. Extracellular pH was measured w...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
