Abstract:
:The effect of endothelial cells (EC) on lymphocyte mitogen responses was examined. Irradiated or mitomycin C treated EC were co-cultured with allogeneic peripheral blood mononuclear cells (PBM), and proliferative responses to pokeweed mitogen (PWM) and phytohemagglutinin (PHA) were assessed by 3H-thymidine incorporation. Compared to lymphocyte responses in the absence of EC, EC co-culture enhanced PWM responses at 72 hours by 55 +/- 28%, 103 +/- 24%, and 96 +/- 9% at EC:PBM ratios of 1:30, 1:10, and 1:3, respectively. The EC co-culture also resulted in significant lymphocyte responses to otherwise submitogenic doses of PWM (10(-4) micrograms/ml) as well as an accelerated kinetics of response. There was no effect of EC on PHA responses. The EC effect appeared not to require cell contact for its expression; however, supernates of EC cultures were not capable of reproducing the effect. On a cell-for-cell basis, EC were more potent in enhancing responses of adherent-cell-depleted lymphocytes than either allogeneic or syngeneic monocytes. Fibroblasts could not substitute for EC in enhancing PWM response, suggesting that the effect was not a nonspecific feeder phenomenon. The EC may play a role in modulating some immune responses in vivo, especially those occurring in areas of inflammation, neovascularization, and endothelial cell proliferation.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Shanahan WR Jr,Weston DM,Korn JHdoi
10.1002/jlb.37.3.305subject
Has Abstractpub_date
1985-03-01 00:00:00pages
305-17issue
3eissn
0741-5400issn
1938-3673journal_volume
37pub_type
杂志文章abstract::Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature myeloid cells that accumulate during pathologic conditions, such as cancer. Patients diagnosed with advanced metastatic cancers have an average survival of 12-24 mo, a survival time that hasn't changed significantly in the past 30 yr. ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.5VMR1116-458RRR
更新日期:2017-09-01 00:00:00
abstract::Chemokine-chemokine receptor (CKR) interactions are traditionally described by a two-step/two-site mechanism that details the major contact points between chemokine ligands and CKRs leading to ligand recognition and receptor activation. Chemokine recognition site 1 (CRS1) encompasses interactions between the CKR N-ter...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.1VMA0218-049R
更新日期:2018-08-01 00:00:00
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1210661
更新日期:2011-09-01 00:00:00
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.52.1.130
更新日期:1992-07-01 00:00:00
abstract::In this study, we analyzed the specific effects of transforming growth factor beta (TGF-beta1) and/or IL-4 on monocyte-derived cells. Monocytes were cultured with GM-CSF, GM-CSF/TGF-beta1, GM-CSF/IL-4, or GM-CSF/IL-4/TGF-beta1 before cell morphology, phenotype, and function were assessed. As expected, interleukin-4 is...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract::Lipopolysaccharide-induced CXC chemokine (LIX) is a novel murine neutrophil-chemoattractant CXC chemokine cloned as a glucocorticoid-attenuated response gene. We investigated LIX message expression in an acute endotoxemia model. LIX message peaks later than KC or macrophage inflammatory protein-2 (MIP-2) and remains e...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.64.4.494
更新日期:1998-10-01 00:00:00
abstract::TNF is a pleiotropic cytokine involved in a variety of inflammatory processes and immune responses. TNF effects are mediated via two distinct membrane receptors: TNFR1 and TNFR2. Investigations concerning regulation and function of TNFR2 revealed a novel TNFR2 isoform in human and mouse cells, termed icp75TNFR, with m...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1007713
更新日期:2008-07-01 00:00:00
abstract::The kinetics and regulatory mechanisms of T cell migration through the endothelium have not been fully defined. In experimental, filter-based assays in vitro, transmigration of lymphocytes takes hours, compared with minutes, in vivo. We cultured endothelial cell (EC) monolayers on filters, solid substrates, or collage...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0508301
更新日期:2009-01-01 00:00:00
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.1A0919-147R
更新日期:2019-12-01 00:00:00
abstract::The transcription factor GATA-2 is expressed in hematopoietic stem and progenitor cells and is functionally implicated in their survival and proliferation. In the present study, we show that GATA-2 exists as an acetylated protein in immature precursor cells, KG1. GATA-2 was acetylated in vitro by p300 and GCN5. We hav...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0603389
更新日期:2004-03-01 00:00:00
abstract::Scaffolding molecules bind simultaneously and link together various components of signal-transduction pathways. Grb2-associated binder 2 (Gab2) is a scaffolding protein required for FcgammaR-initiated allergic responses in mast cells and FcgammaR-mediated phagocytosis in macrophages, where it links IgE and IgG recepto...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0304179
更新日期:2004-10-01 00:00:00
abstract::A role for the dietary trace mineral element selenium in the reduction of cancer incidence has been documented in numerous epidemiological and experimental studies. The precise mechanism of this antitumor effect is not well understood, but published data suggest that both inhibition of tumor cell growth and enhancemen...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.45.3.207
更新日期:1989-03-01 00:00:00
abstract::PI3Ks have been identified as key signaling proteins involved in many basic biologic processes in health and disease. Transgenic animals have been essential tools to study the underlying molecular mechanisms in this context and therefore, have been widely used to elucidate the role of these factors in many different s...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.4HI0316-157R
更新日期:2017-02-01 00:00:00
abstract::Absence of lymph nodes in nonmammalian species, expression of MHCII by APCs in the periphery, and the recent findings that T cells can change their polarization status after presentation in the lymph nodes imply a role for MHCII-mediated presentation outside the organized lymphoid tissue. This study shows that MHCII(+...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0310161
更新日期:2010-09-01 00:00:00
abstract::Microorganisms interact with the immune system in multiple ways. In an interaction between a microorganism and its host, the defense of the host does not go unchallenged. Microorganisms have for decades been suspected of possessing the capabilities of hiding from and escaping the consequences of immune surveillance. E...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1002/jlb.62.4.415
更新日期:1997-10-01 00:00:00
abstract::Leukocytes have been implicated to be involved in the pathogenesis of IgA nephropathy (IgAN). To clarify the precise molecular mechanism of recruitment and activation of leukocytes in the subgroups of IgAN, latent, acute, and chronic types, we studied monocyte chemotactic and activating factor (MCAF/MCP-1) and interle...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.63.4.493
更新日期:1998-04-01 00:00:00
abstract::We reported that PARP-1 exhibits differential roles in expression of inflammatory factors. Here, we show that PARP-1 deletion was associated with a significant reduction in inflammatory cell recruitment to mouse airways upon intratracheal administration of LPS. However, PARP-1 deletion exerted little effect in respons...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0309183
更新日期:2009-12-01 00:00:00
abstract::Ly-6G is a member of the Ly-6 family of GPI-linked proteins, which is expressed on murine neutrophils. Antibodies against Ly-6G cause neutropenia, and fatal reactions also develop if mice are primed with TNF-alpha prior to antibody treatment. We have investigated the mechanisms behind these responses to Ly-6G ligation...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0507305
更新日期:2009-01-01 00:00:00
abstract::In recent years, it has become appreciated that immune cells have different metabolic profiles depending on their activation status. During sepsis, circulating leukocytes go through a hyperinflammatory state, which can be accompanied or followed by defective antimicrobial defenses (also described as immune tolerance o...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.4MR0216-066R
更新日期:2017-01-01 00:00:00
abstract::The role of JNK in neutrophil chemotaxis and killing of microbial pathogens remains unclear. Using a recently described cell-permeable peptide inhibitor of the JNK pathway, based on the JBD of JIP-1, coupled to the protein transduction domain of HIV-TAT (TAT-JIP), in association with control peptides, we demonstrate t...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0609399
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abstract::The neutrophil inflammatory response can be altered profoundly by contact with extracellular matrix proteins (ECMs). We characterized functional responses (intracellular calcium, actin polymerization, degranulation, adhesion, and oxidative burst) of bovine neutrophils adhered to selected ECM proteins [collagen IV, lam...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract::Inflammation is deeply entangled with redox modulation. Triggering of PRRs on inflammatory cells induces ROS generation. As a consequence, activated cells mount antioxidant responses to counteract the possible harmful effects of oxidation. Therefore, when repair is completed, homeostasis is restored. Here, we describe...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.1008598
更新日期:2009-09-01 00:00:00
abstract::GM-CSF is well recognized as a proliferative agent for hematopoietic cells and exerts a priming function on neutrophils. The aim of this study was to determine if GM-CSF has a role as a neutrophil chemoattractant in vivo and if it can contribute to recruitment during intestinal inflammation. Initial studies in vitro, ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0809546
更新日期:2011-06-01 00:00:00
abstract::Thimerosal is an organic mercury compound that is used as a preservative in vaccines and pharmaceutical products. Recent studies have shown a TH2-skewing effect of mercury, although the underlying mechanisms have not been identified. In this study, we investigated whether thimerosal can exercise a TH2-promoting effect...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0706467
更新日期:2007-02-01 00:00:00
abstract::Prevalence of food allergies in the United States is on the rise. Eosinophils are recruited to the intestinal mucosa in substantial numbers in food allergen-driven gastrointestinal (GI) inflammation. Soluble epoxide hydrolase (sEH) is known to play a pro-inflammatory role during inflammation by metabolizing anti-infla...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.3MA1017-423R
更新日期:2018-07-01 00:00:00
abstract::Regulation of macrophage PCD plays an important role in pathogenesis of leishmaniasis. However, the precise involvement of any parasite molecule in this process remains uncertain. In the current study, in silico wide analysis demonstrated that genes in the Leishmania donovani genome are highly enriched for CpG motifs,...
journal_title:Journal of leukocyte biology
pub_type: 临床试验,杂志文章
doi:10.1189/jlb.4A0713-378RR
更新日期:2015-02-01 00:00:00
abstract::Feline peritoneal cells were collected by lavage with isotonic saline without the use of irritants or need for euthanasia of the cats. Macrophages were purified by centrifugation on Percoll followed by selective adherence. Although few macrophages could be obtained from an initial lavage, a second lavage performed on ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.44.5.319
更新日期:1988-11-01 00:00:00
abstract::Phagocytosis is the process whereby cells engulf large particles, usually over 0.5 micro m in diameter. Phagocytosis is triggered by the interaction of opsonins that cover the particle to be internalized with specific receptors on the surface of the phagocyte. The best-studied phagocytic receptors include the Fc recep...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:
更新日期:2002-12-01 00:00:00
abstract::In an analysis of nitric oxide (.NO) production and toxicity, chicken macrophage-generated .NO inhibited mitochondrial activity in both .NO-producing macrophages themselves and lymphoid tumor targets. However, differences in targeting of mitochondrial toxicity were observed among these cells. Two chicken macrophage ce...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.56.1.52
更新日期:1994-07-01 00:00:00