Abstract：
:A circular form of bovine pancreatic trypsin inhibitor (BPTI) has been prepared by introducing a peptide bond between the N- and C-termini, which are in close proximity in the native conformation. The pathway and energetics of the disulphide-coupled folding transition of the circular protein have been studied using methods applied previously to the unmodified protein. The cross-link between the termini was found not to significantly stabilize the native state in spite of the expected reduction in entropy of the unfolded protein. This unexpected result has led to a reexamination of the stabilization expected from a cross-link, considering effects on the native, as well as unfolded, states of the protein. The greatest stabilization is expected when the cross-linked groups are held rigidly in the native protein in the optimum orientation for forming the cross-link. Similar analyses, utilizing thermodynamic cycles, can be applied to other interactions that stabilize native proteins, including disulphide bonds, salt bridges, and hydrogen bonds and to modifications to the protein that remove them. In general, the contribution of an individual interaction to the stability of the native state depends on the extent to which the interaction is favored in the native conformation, which can vary greatly depending on the local environment of the interacting groups.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Goldenberg DPdoi
10.1002/jcb.240290406subject
Has Abstractpub_date
1985-01-01 00:00:00pages
321-35issue
4eissn
0730-2312issn
1097-4644journal_volume
29pub_type
杂志文章,评审abstract：:The mouse macrophage Fc gamma 2b/gamma 1R has previously been purified with the aid of the monoclonal antibody 2.4G2. That this Fc gamma R functions as a ligand-dependent ion channel is supported by the following evidence. Employing [3H]tetraphenylphosphonium ([3H]Ph4P+) as a probe for membrane potential changes in in...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.240290403
更新日期：1985-01-01 00:00:00
abstract：:Oxoglutarate receptor 1 (OXGR1), as one of the intermediates in G protein-coupled receptors (GPCRs), plays a crucial role in the citric acid cycle receptor of α-ketoglutarate and metabolism. GPCR can control the cell proliferation by regulating the downstream signaling of G protein signaling pathways. The PI3K/AKT pat...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27970
更新日期：2019-04-01 00:00:00
abstract：:The testis-specific linker histone H1t gene is transcribed exclusively in pachytene primary spermatocytes. Tissue specific expression of the gene is mediated in part by transcriptional factors that bind elements located within the proximal and distal promoter. A 40 bp promoter element, designated H1t/TE, that is locat...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.10468
更新日期：2003-04-15 00:00:00
abstract：:Lysozyme is expressed in serous, but not mucous, cells of the tracheobronchial glands and thereby constitutes a marker of the serous cell lineage in these glands. To identify DNA regulatory elements and transcription factors mediating the commitment of progenitor cells to the serous cell lineage, we have characterized...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4644(19960601)61:3%3C350::AID-J
更新日期：1996-06-01 00:00:00
abstract：:The pathogenesis of hyperprolactinemia (hyperPRL) induced hypogonadism has been suggested to be related with a dysfunction of hypothalamus-pituitary-testis axis. While the direct inhibitory effects of prolactin (PRL) on testosterone (T) release have been demonstrated, the mechanism is still unclear. Our previous study...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/1097-4644(20010301)80:3<313::aid-jcb30>3.0
更新日期：2001-01-01 00:00:00
abstract：:Focal adhesion kinase (FAK) is a structurally unique nonreceptor protein-tyrosine kinase that localizes to focal adhesion plaques. Regulation of its activity has been implicated in diverse signaling pathways, including those mediated by extracellular matrix/integrin interactions, G-protein coupled receptors for mitoge...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240550113
更新日期：1994-05-01 00:00:00
abstract：:The BRCA1 tumor suppressor involved in breast and ovarian cancer is linked to several fundamental cell regulatory processes. Recently, it was reported that BRCA1 supports localization of XIST RNA to the inactive X chromosome (Xi) in women. The apparent cytological overlap between BRCA1 and XIST RNA across the Xi raise...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21188
更新日期：2007-03-01 00:00:00
abstract：:The efficiency of in vitro mesenchymal stem cell (MSC) differentiation into the myocardial lineage is generally poor. In order to improve cardiac commitment, bone marrow GFP+MSCs obtained from transgenic rats were cultured with adult wild type rat cardiomyocytes for 5 days in the presence of difluoromethylornithine (D...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21683
更新日期：2008-03-01 00:00:00
abstract：:Transcription factor Sp-1 is an important fibrogenic factor that is involved in the pathogenesis of diabetic nephropathy. In this study, we examined the effect of Sp1 decoy oligodeoxynucleotides (ODNs) on the extracellular matrix (ECM) gene expression in cultured rat mesangial cells (RMC) and streptozotocin (STZ)-indu...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21440
更新日期：2008-02-01 00:00:00
abstract：BACKGROUND:Long noncoding RNAs (lncRNAs) regulate gene expression by acting with microRNAs (miRNAs) and indirectly interact with messenger RNA (mRNAs). However, the roles of specific lncRNA and its related competing endogenous RNAs (ceRNA) network in alopecia areata (AA) are not fully understood. METHODS:The blood lnc...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28365
更新日期：2019-06-01 00:00:00
abstract：:Fibroblast growth factor 23 (FGF23) is a phosphaturic hormone produced by bone and exerts its function in the target organs by binding the FGF receptor (FGFR) and Klotho. Since recent studies suggested that extracellular inorganic phosphate (Pi) itself triggers signal transduction and regulates gene expression in some...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22842
更新日期：2010-12-01 00:00:00
abstract：:Plasminogen activator inhibitor type-1 (PAI-1), a major regulator of the plasmin-dependent pericellular proteolytic cascade, is prominently expressed during the tissue response to injury although the factors that impact PAI-1 induction and their role in the repair process are unclear. Kinetic modeling using establishe...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.24861
更新日期：2014-10-01 00:00:00
abstract：:Toxoplasma gondii excreted-secreted antigens (ESA) could lead to the fetal abortion especially in the early stage of pregnancy. Deficit in regulatory T cells is a critical event in the fetal abortion. Transcription factor forkhead box p3 (Foxp3) mediates differentiation and functional roles on regulatory T cells. Prev...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27358
更新日期：2018-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:In this study, we aimed to study the molecular mechanisms underlying the symptoms of hyperresponsiveness during intubation. METHOD:The value of circulating long noncoding RNA (lncRNA)-prognosis-associated gallbladder cancer (PAGBC) in the prediction of hyperresponsiveness upon intubation duri...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27513
更新日期：2018-09-23 00:00:00
abstract：:This study aims to explore how microRNA-133a (miR-133a) affects cell apoptosis and radio-sensitivity by targeting EGFR via regulating MEK/ERK pathway in esophageal cancer (EC). A total of 358 EC patients were selected and assigned into the resistant and sensitive groups. Human EC KYSE 150 cell line was assigned into t...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.25829
更新日期：2017-09-01 00:00:00
abstract：:The hypothesis that intercellular adhesion can be subdivided into two separable phenomena, an initial recognition event and a subsequent stabilization, is supported by the use of a new cell binding assay that provides a quantitative measure of intercellular binding strengths. Radioactive single cells are brought into ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.1982.240180408
更新日期：1982-01-01 00:00:00
abstract：:3-Acetyl-11-keto-β-boswellic acid (AKBA), a pentacyclic triterpenic acid present in gum resin of Boswellia serrata, has been found to possess antioxidant and neuroprotective properties. In this study, we aimed to examine protective properties of AKBA against glutamate-induced neuronal injury. To investigate the effect...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.29413
更新日期：2020-02-01 00:00:00
abstract：:The expression of matrix metalloproteinase-13 (MMP-13), involved in bone turnover, is elevated in stretched MC3T3-E1 osteoblast-like cells. Strain-mediated forces impact bone remodeling due in large part to the movement of fluid through the canalicular-lacunar network. The resulting fluid shear stress (FSS) over the s...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21349
更新日期：2007-12-01 00:00:00
abstract：:Lack of guidelines for personalized chemotherapy treatment after surgery has caused gastric cancer (GC) patients' unnecessary exposure to toxicity and the financial burden of chemotherapy treatments. In our study, we aimed to identify potential biomarkers to predict GC patients' susceptibility to platinum-based on Gen...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28621
更新日期：2019-08-01 00:00:00
abstract：:Recent ultrastructural, immunoelectron, and confocal microscopy observations done in our laboratory [Barboro et al. [2002] Exp. Cell. Res. 279:202-218] have confirmed that lamins and the nuclear mitotic apparatus protein (NuMA) are localized inside the interphase nucleus in a polymerized form. This provided evidence o...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.10378
更新日期：2003-01-01 00:00:00
abstract：:Common differentially expressed genes (DEGs) in acute lymphoblastic leukemia (ALL) and chronic lymphocytic leukemia (CLL) might play critical roles in the pathogenesis and process of leukemia. We collected RNA sequencing (RNA-seq) data of human CLL, ALL samples, and normal peripheral blood CD19+ B cells as well as thy...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28876
更新日期：2019-09-01 00:00:00
abstract：:Prostate cancer is a heterogeneous disease and thus, it is important to understand whether among the heterogeneous collection of cell types, androgen-deprivation insensitive cells exist prior to hormonal manipulation. We established several LNCaP subclones with distinct insensitivities to androgen deprivation from a p...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.24805
更新日期：2014-09-01 00:00:00
abstract：:Osteoclasts are highly differentiated terminal cells formed by fusion of hematopoietic stem cells. Previously, osteoprotegerin (OPG) inhibit osteoclast differentiation and bone resorption by blocking receptor activator of nuclear factor-κB ligand (RANKL) binding to RANK indirect mechanism. Furthermore, autophagy plays...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27468
更新日期：2018-09-06 00:00:00
abstract：:The study was conducted for comparing the effects of 12 DNA damage response gene mutations (CHEK1, CHEK2, RAD51, BRCA1, BRCA2, MLH1, MSH2, ATM, ATR, MDC1, PARP1, and FANCF) on the overall survival (OS) of breast cancer (BC) patients. We searched the Cancer Genome Atlas (TCGA) database from inception to September 2016....
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,meta分析
doi：10.1002/jcb.26140
更新日期：2017-12-01 00:00:00
abstract：:Cerebral ischemia/reperfusion (I/R) typically occurs after mechanical thrombectomy to treat ischemic stroke, generation of reactive oxygen species (ROS) after reperfusion may result in neuronal insult, ultimately leading to disability and death. Regulated in development and DNA damage responses 1 (REDD1) is a conserve...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28737
更新日期：2019-09-01 00:00:00
abstract：:Malignant gliomas, the most common subtype of primary brain tumors, are characterized by high proliferation, great invasion, and neurological destruction and considered to be the deadliest of human cancers. Analgesic-antitumor peptide (AGAP), one of scorpion toxic polypeptides, has been shown to have antitumor activit...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.23166
更新日期：2011-09-01 00:00:00
abstract：:This review point out several aspects regarding the mitogen-activated protein kinase (MAPK)/extracellular-regulated kinase (Erk) network, which are still pending issues in the understanding how this pathway integrate information to drive cell fates. Focusing on the role of Erk during cell cycle, it has to be underline...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.22477
更新日期：2010-04-01 00:00:00
abstract：:Adult human mesenchymal stem cells (MSCs) hold promise for an increasing list of therapeutic uses due to their ease of isolation, expansion, and multi-lineage differentiation potential. To maximize the clinical potential of MSCs, the underlying mechanisms by which MSC functionality is controlled must be understood. We...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22289
更新日期：2009-10-15 00:00:00
abstract：:Resident T lymphocytes have been found to exert helper and suppressor regulatory influences with regard to polyclonal activation of murine splenic B lymphocytes elicited by lipopolysaccharide. In the normal adult spleen, only T cell helper influences are exercised over polyclonal B cell activation. This activity is a ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.1982.240180402
更新日期：1982-01-01 00:00:00
abstract：:In addition to its roles in sugar metabolism, fructose-1,6-bisphosphate aldolase (aldolase) has been implicated in cellular functions independent from these roles, termed "moonlighting functions." These moonlighting functions likely involve the known aldolase-actin interaction, as many proteins with which aldolase int...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.24538
更新日期：2013-08-01 00:00:00
