Abstract：
INTRODUCTION:Identification of yeasts is based on morphological, biochemical and nutritional characteristics, and using molecular methods. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, a new method for the identification of microorganisms, has demonstrated to be very useful. The aim of this study is to evaluate this new method in the identification of yeasts. METHODS:A total of 600 strains of yeasts isolated from clinical specimens belonging to 9 genera and 43 species were tested. Identification was made by sequencing of the ITS regions of ribosomal DNA, assimilation of carbon compounds (ID 32C), and mass spectrometry on a Microflex spectrometer (Bruker Daltonics GmbH, Germany). RESULTS:A total of 569 strains (94.8%) were identified to species level by ID 32C, and 580 (96.7%) by MALDI-TOF. Concordance between both methods was observed for 553 strains (92.2%), with 100% in clinically relevant species: C. albicans, C. glabrata, C. parapsilosis, C. tropicalis, and almost 100% in C. krusei. MALDI-TOF identified species requiring molecular methods: Candida dubliniensis, C. nivariensis, C. metapsilosis and C. orthopsilosis. Some irregularities were observed in the identification of arthroconidia yeast and basidiomycetes. CONCLUSION:MALDI-TOF is a rapid, effective and economic method, which enables the identification of most clinically important yeasts and the differentiation of closely related species. It would be desirable to include more species in its database to expand its performance.
journal_name
Enferm Infecc Microbiol Clinjournal_title
Enfermedades infecciosas y microbiologia clinicaauthors
Galán F,García-Agudo L,Guerrero I,Marín P,García-Tapia A,García-Martos P,Rodríguez-Iglesias Mdoi
10.1016/j.eimc.2014.10.003subject
Has Abstractpub_date
2015-06-01 00:00:00pages
372-8issue
6eissn
0213-005Xissn
1578-1852pii
S0213-005X(14)00340-1journal_volume
33pub_type
杂志文章abstract：:Although influenza is usually a self-limited disease, patients who develop complications are at increased risk of hospitalization, intensive care unit admission and death. Since preventive and early therapeutic measures should be prioritized in higher risk patients, identification of the risk factors for severe infect...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/S0213-005X(12)70102-7
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abstract：INTRODUCTION:The aim of this study was to describe the clinical and epidemiologic features of Q fever in the southern area of the island of Gran Canaria (Spain). METHODS:We conducted a retrospective analysis of the clinical and epidemiological data of the cases of Q fever diagnosed by the Microbiology Laboratory of th...
journal_title：Enfermedades infecciosas y microbiologia clinica
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更新日期：2003-01-01 00:00:00
abstract：INTRODUCTION:To describe clinical features, complications, serotypes and antibiotic resistance in pneumococcal pneumonia in our environment after the generalization of the heptavalent conjugate vaccine (PCV-7) in paediatrics. MATERIAL AND METHODS:Prospective study of episodes of pneumococcal pneumonia, with positive c...
journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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abstract：:The HIV replication cycle passes through a stage of integrating proviral DNA into the cell's DNA. In this process, the viral enzyme, integrase, catalyses two reactions. The first reaction, which seems to occur in the cytoplasm, involves 3'-end processing, in which two nucleotides are removed from the 3' ends of the vi...
journal_title：Enfermedades infecciosas y microbiologia clinica
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abstract：BACKGROUND:Visceral leishmaniasis (VL) is an endemic parasitosis in Spain with a frequency which is increasing, specially among those patients with HIV infection. METHODS:The clinical characteristics of 36 episodes of VL in 20 patients with HIV infection diagnosed in the authors' center from January 1988 to October 19...
journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：
更新日期：1995-02-01 00:00:00
abstract：INTRODUCTION:Factors predicting short-term amputation during hospital treatment of patients admitted for acute diabetic foot infections are of interest for clinicians managing the acute episode. METHODS:A retrospective clinical records analysis of 78 consecutive patients hospitalized for acute diabetic foot infections...
journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 共识发展会议,杂志文章,实务指引
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journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：
更新日期：1989-01-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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更新日期：2017-01-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：
更新日期：1990-03-01 00:00:00
abstract：BACKGROUND:HIV infection and parenteral drug abuse (PDA) are frequently associated conditions. Both are at increased risk of developing specific types of Candida infections. Localized CNS infection is an exceptional finding. METHODS:We report two cases of meningitis in PDA due to Candida albicans--one of them HIV posi...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：
更新日期：1993-05-01 00:00:00
abstract：INTRODUCTION:the duration adequacy of antibiotic regimens is one of the key points of Antimicrobial Stewardship Programs (ASP) given the relationship between the risk of resistance and days of exposure to antimicrobials. METHODS:monitoring activities of intravenous antibiotics longer than 7 days at Hospital Infantil V...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2020.02.015
更新日期：2020-03-27 00:00:00
abstract：AIMS:Francisella tularensis is a facultative intracellular gramnegative aerobic coccobacilli which is difficult to isolate. The appearance of an epidemic outbreak of tularemia in Castilla y Leon (Spain) during the first months of 1998 led bur microbiology laboratory to develop the diagnostic methods of this disease whi...
journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：
更新日期：1999-11-01 00:00:00
abstract：BACKGROUND:Plasma HIV p24 is considered a significant predictor of CD4+ T cell decline and progression to AIDS in HIV-infected patients. We evaluated the p24 levels in patients on triple therapy and after switching to ritonavir-boosted protease inhibitor monotherapy (mtPI/rtv), as well as the relationships with virolog...
journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：
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journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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更新日期：2011-03-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
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更新日期：2008-11-01 00:00:00
abstract：BACKGROUND:Streptococcus agalactiae or streptococcus group B (SGB) is the main etiologic agent of early neonatal sepsis. A multicenter study was performed with the aim of determining the incidence and characteristics of this disease in our medium and contribute the design of an adequate prevention protocol. METHODS:Te...
journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：
更新日期：1998-08-01 00:00:00
abstract：OBJECTIVE:To determine the prevalence of chronic colonization with methicillin-resistant Staphylococcus aureus (MRSA) in patients with cystic fibrosis, describe antibiotic sensitivity of the strains, and compare the patients' clinical characteristics with those of patients infected with methicillin-sensitive S. aureus ...
journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2015.04.004
更新日期：2016-03-01 00:00:00
abstract：:Intraabdominal infections are severe with a high morbidity and mortality which may be produced by multiple causes: perforation of the empty viscera, intestinal inflammatory processes, vascular pictures, abdominal traumatisms as a consequence of surgery. From a microbiologic point of view, different types of gram posit...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:Mycobacterial culture has a high sensitivity and is the test of choice for the microbiological diagnosis of tuberculosis and nontuberculous mycobacterial infections. However, the results of this culture require at least 2-3 weeks to obtain positivity. Staining is rapid and can be used as a complementary study, althoug...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2017.11.009
更新日期：2018-02-01 00:00:00
abstract：BACKGROUND:Staphylococcus aureus is the main pathogen causing nosocomial infections. Health professionals, including medical students, could be a source of transmission. The aims of the study were to determine the rate of nasal carriage of S.aureus susceptible and resistant to methicillin (MRSA) and evaluate the knowle...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2012.12.005
更新日期：2013-10-01 00:00:00
