8-azaguanosine-5'-monophosphate synthesis via nucleoside kinase in cultured Chinese hamster lung fibroblasts.

Abstract:

:Cultured chinese hamster lung fibroblasts, and a variant clone selected for resistance to 8-azaguanine, that lacks hypoxanthine-guanine phosphoribosyl transferase (EC 2.4.2.8), have been tested for the ability to convert 8-azaguanine into 8-azaguanosine-5'-monophosphate via purine nucleoside phosphorylase and nucleoside kinase. Purine nucleoside phosphorylase of both cell types is able to synthesize 8-azaguanosine from 8-azaguanine with the same efficiency. Wild type cells possess a nucleoside kinase activity acting on 8-azaguanosine, but this activity is considerably lower in the cells displaying resistance to the base analog. Our lines of evidence demonstrate that purine nucleoside phosphorylase and nucleoside kinase constitute a possible way of synthesis of the cytotoxic mononucleotide of 8-azaguanine, and, in fact, cells selected for resistance to the base analog show an impairement in the nucleoside kinase activity.

authors

Tozzi MG,Camici M,Falcone V,Turriani M,Turchi G,Ipata PL

doi

10.1016/0006-291x(89)90073-9

subject

Has Abstract

pub_date

1989-03-15 00:00:00

pages

854-61

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(89)90073-9

journal_volume

159

pub_type

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