Abstract:
:Epithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy. On the basis of its histopathology and molecular-genomic changes, ovarian cancer has been divided into subtypes, each with distinct biology and outcome. The aim of this study was to develop a panel of patient-derived EOC xenografts that recapitulate the molecular and biologic heterogeneity of human ovarian cancer. Thirty-four EOC xenografts were successfully established, either subcutaneously or intraperitoneally, in nude mice. The xenografts were histologically similar to the corresponding patient tumor and comprised all the major ovarian cancer subtypes. After orthotopic transplantation in the bursa of the mouse ovary, they disseminate into the organs of the peritoneal cavity and produce ascites, typical of ovarian cancer. Gene expression analysis and mutation status indicated a high degree of similarity with the original patient and discriminate different subsets of xenografts. They were very responsive, responsive, and resistant to cisplatin, resembling the clinical situation in ovarian cancer. This panel of patient-derived EOC xenografts that recapitulate the recently type I and type II classification serves to study the biology of ovarian cancer, identify tumor-specific molecular markers, and develop novel treatment modalities.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Ricci F,Bizzaro F,Cesca M,Guffanti F,Ganzinelli M,Decio A,Ghilardi C,Perego P,Fruscio R,Buda A,Milani R,Ostano P,Chiorino G,Bani MR,Damia G,Giavazzi Rdoi
10.1158/0008-5472.CAN-14-0274subject
Has Abstractpub_date
2014-12-01 00:00:00pages
6980-90issue
23eissn
0008-5472issn
1538-7445pii
0008-5472.CAN-14-0274journal_volume
74pub_type
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