Esterase electrophoresis: a molecular tool for studying the epidemiology of Branhamella catarrhalis nosocomial infection.

Abstract:

:A new epidemiologic typing method based on electrophoresis of esterases had been developed for differentiating between clinical isolates of Branhamella catarrhalis. Twenty-two epidemiologically significant strains obtained from three Chest Units, a Paediatric Intensive Care Unit and a Paediatric Unit were compared with 54 randomly selected strains and 4 reference strains, including the species type strain, ATCC 25238. Thirty-four distinct zymotypes were characterized by polyacrylamide-agarose gel electrophoresis of the 80 strains. One infrequent zymotype was found in 2 neonates and another in 2 adults with nosocomial bronchopulmonary infections, suggesting the nosocomial spread of 2 outbreak strains of B. catarrhalis. A more frequent zymotype was isolated from 3 neonates with nosocomial bronchopulmonary infection and from 2 children with nosocomial rhinopharyngitis. The remaining 12 epidemiologically significant strains were of varied zymotypes. This work demonstrates that esterase electrophoresis is a suitable, readily reproducible, stable typing system applicable to the wide range of strains found in B. catarrhalis nosocomial infections.

journal_name

Epidemiol Infect

authors

Picard B,Goullet P,Denamur E,Suermondt G

doi

10.1017/s0950268800030946

subject

Has Abstract

pub_date

1989-12-01 00:00:00

pages

547-54

issue

3

eissn

0950-2688

issn

1469-4409

journal_volume

103

pub_type

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