Abstract:
:The neurotoxicity of levodopa (L-DOPA) on neural stem cells (NSCs) and treatment strategies to protect NSCs from this neurotoxicity remain to be elucidated. Recently, an active form of vitamin D3 has been reported to display neuroprotective properties. Therefore, we investigated the protective effect of 1,25-dyhydroxyvitamin D3 (calcitriol) on L-DOPA-induced NSC injury. We measured cell viability via the cell counting kit-8 (CCK-8) and lactate dehydrogenase (LDH) assays and Annexin V/PI staining followed by flow cytometry, cell proliferation using the BrdU and colony-forming unit (CFU) assays, cell differentiation via immunocytochemistry, the levels of free radicals via 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) staining, apoptosis via DAPI and TUNEL staining, and intracellular signaling protein expression via Western blot. Antibody microarrays were also employed to detect changes in the expression of prosurvival- and death-related proteins. Treatment of NSCs with L-DOPA reduced their viability and proliferation. This treatment also increased the levels of free radicals and decreased the expression levels of intracellular signaling proteins that are associated with cell survival. However, simultaneous exposure to calcitriol significantly reduced these effects. The calcitriol-mediated protection against L-DOPA toxicity was blocked by the phosphoinositide 3-kinase (PI3K) inhibitor LY294004. L-DOPA also inhibited the expression of Nestin and Ki-67, and co-treatment with calcitriol alleviated these effects. The expression levels of GFAP, DCX, and Tuj1 were not significantly affected by treatment with L-DOPA or calcitriol. Calcitriol protects against L-DOPA-induced NSC injury by promoting prosurvival signaling, including activation of the PI3K pathway, and reducing oxidative stress.
journal_name
Mol Neurobioljournal_title
Molecular neurobiologyauthors
Jang W,Park HH,Lee KY,Lee YJ,Kim HT,Koh SHdoi
10.1007/s12035-014-8835-1subject
Has Abstractpub_date
2015-04-01 00:00:00pages
558-70issue
2eissn
0893-7648issn
1559-1182journal_volume
51pub_type
杂志文章abstract::The effects and mechanism of action of oligodeoxyribonucleotides containing CpG motif (CpG-ODNs) on neuron cells are largely unexamined. Here, we found that CpG-A ODNs but not other types of CpG-ODNs induced neurite retraction and cell apoptosis of rat embryonic neurons in a TLR9-independent manner. These effects of C...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-012-8275-8
更新日期:2012-06-01 00:00:00
abstract::Neurogenesis is a developmental process that involves fine-tuned coordination between self-renewal, proliferation, and differentiation of neural stem cells (NSCs) into neurons. However, early-life assault with environmental toxicants interferes with the regular function of genes, proteins, and other molecules that bui...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-020-02108-9
更新日期:2021-01-01 00:00:00
abstract::Neurodegenerative foldopathies are characterized by aberrant folding of diseased modified proteins, which are major constituents of the intracellular and extracellular lesions. These lesions correlate with the cognitive and/or motor impairment seen in these diseases. The majority of the disease modified proteins in ne...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-013-8440-8
更新日期:2013-12-01 00:00:00
abstract::Ketamine is used as a general anesthetic, and recent data suggest that anesthetics can cause neuronal damage when exposure occurs during development. The precise mechanisms are not completely understood. To evaluate the degree of ketamine-induced neuronal toxicity, neural stem cells were isolated from gestational day ...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-015-9248-5
更新日期:2015-10-01 00:00:00
abstract::Cyclin-dependent kinase 5 regulatory subunit 1 (CDK5R1) encodes p35, the main activatory subunit of cyclin-dependent kinase 5 (CDK5). The p35/CDK5 active complex plays a fundamental role in brain development and functioning, but its deregulated activity has also been implicated in various neurodegenerative disorders, ...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-016-0002-4
更新日期:2017-08-01 00:00:00
abstract::Epigallocatechin-3-gallate (EGCG), a catechin found in green tea, has been previously investigated for its neuroprotective effects in vitro and in vivo. In the present study, we aimed to evaluate its possible beneficial effects in a well-established preclinical mixed model of familial Alzheimer's disease (AD) and type...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-019-01849-6
更新日期:2020-04-01 00:00:00
abstract::Voltage-gated calcium channels (VGCCs) play a critical role in neuroinflammatory diseases, such as multiple sclerosis (MS). CTK 01512-2 is a recombinant version of the peptide Phα1β derived from the spider Phoneutria nigriventer, which inhibits N-type VGCC/TRPA1-mediated calcium influx. We investigated the effects of ...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-018-1049-1
更新日期:2018-12-01 00:00:00
abstract::Polyglutamine (polyQ) disorders share many similarities, such as a common mutation type in unrelated human causative genes, neurological character, and certain aspects of pathogenesis, including morphological and physiological neuronal alterations. The similarities in pathogenesis have been confirmed by findings that ...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-012-8315-4
更新日期:2012-10-01 00:00:00
abstract::For more than a century dendritic spines have been a source of fascination and speculation. The long-held belief that these anatomical structures are involved in learning and memory are addressed. Specifically, two lines of evidence that support this claim are reviewed. In the first, we review evidence that experiment...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1385/MN:29:2:117
更新日期:2004-04-01 00:00:00
abstract::Stromal cell-derived factor-1 alpha (SDF-1a) or CXCL12 is an important cytokine with multiple functions in the brain during development and in adulthood. The inflammatory response initiated by spinal cord injury (SCI) involves the processing of interleukin-1beta (IL-1ß) and IL-18 mediated by caspase-1 which is under t...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-015-9203-5
更新日期:2016-07-01 00:00:00
abstract::Klotho protein, encoded by the Klotho gene (KL) at locus 13q12, is an antiaging hormone-like protein playing a pivotal role in cell metabolism homeostasis and associated to longevity and age-related diseases. In particular, altered cell metabolism in central nervous system may influence the behavior of serotoninergic ...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-016-9711-y
更新日期:2017-03-01 00:00:00
abstract::Radiation necrosis (RN) in brain tumor patients is often symptomatic, persistent without immediate resolution, and confused with tumor recurrence. Cerebral vascular pericytes are essential for endothelial function, vascular integrity, and angiogenesis. In this study, we showed that the loss of pericytes is involved in...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-017-0695-z
更新日期:2018-06-01 00:00:00
abstract::Chronic hyperammonemia impairs spatial memory by altering membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus. Intracerebral administration of extracellular cGMP to hyperammonemic rats restores spatial memory and membrane expression of AMPA receptors. The underlying molecular mechanisms re...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-018-1387-z
更新日期:2019-06-01 00:00:00
abstract::Tonic GABAA receptors are a subpopulation of receptors that generate long-lasting inhibition and thereby control network excitability. In recent years, these receptors have been implicated in various neurological and psychiatric disorders, including Parkinson's disease, schizophrenia, and epilepsy. Their distinct subu...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-015-9423-8
更新日期:2016-10-01 00:00:00
abstract::L-DOPA is the main pharmacological therapy for Parkinson's disease. However, long-term exposure to L-DOPA induces involuntary movements termed dyskinesia. Clinical trials show that dyskinesia is attenuated by metabotropic glutamate receptor type 5 (mGluR5) antagonists. Further, the onset of dyskinesia is delayed by ni...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-018-1356-6
更新日期:2019-06-01 00:00:00
abstract::Most survivors of ischemic stroke remain physically disabled and require prolonged rehabilitation. However, some stroke victims achieve a full neurological recovery suggesting that the human brain can defend itself against ischemic injury, but the protective mechanisms are unknown. This study used selective pharmacolo...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-016-9796-3
更新日期:2017-04-01 00:00:00
abstract::Depression is the leading cause of disability worldwide with global distribution of 322 million people suffering from the disease. While much is understood about depression, the underlying pathophysiology is yet to be fully characterized. Recently, the unfolded protein response (UPR) has been shown to be involved in r...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-019-01687-6
更新日期:2019-12-01 00:00:00
abstract::Genetic disruptions of spindle/centrosome-associated WD40-repeat protein 62 (WDR62) are causative for autosomal recessive primary microcephaly (MCPH) and a broader range of cortical malformations. Since the identification of WDR62 as encoded by the MCPH2 locus in 2010, recent studies that have deleted/depleted WDR62 i...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-017-0778-x
更新日期:2018-07-01 00:00:00
abstract::Recently, deregulated microRNA (miRNA) expression contributes to the development and progression of human glioblastoma. The aim of the present study was to evaluate the level of miR-154 and Wnt5a in glioblastoma tissues and cells. We further investigated the molecular mechanisms of miR-154 and Wnt5a in glioblastoma ce...
journal_title:Molecular neurobiology
pub_type: 杂志文章,收录出版
doi:10.1007/s12035-016-9867-5
更新日期:2017-05-01 00:00:00
abstract::It is important to understand the molecular mechanisms of barrier disruption in the central nervous system (CNS) of patients with multiple sclerosis (MS). The purpose of the present study was to clarify whether claudin-11 is involved in the disruption of two endothelial barriers (blood-brain barrier (BBB) and blood-sp...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-018-1207-5
更新日期:2019-03-01 00:00:00
abstract::The striatum is divided into two compartments, the striosomes and extrastriosomal matrix, which differ in several cytochemical markers, input-output connections, and time of neurogenesis. Since it is thought that limbic, reward-related information and executive aspects of behavioral information may be differentially p...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-008-8023-2
更新日期:2008-04-01 00:00:00
abstract::This study examined the effects of pinealectomy in Wistar rats and melatonin replacement therapy on the daily mRNA expression of melatonin (Tph1, Aanat, Asmt, Mt1, Mt2, and Rorα), and steroidogenic (Star, 17βhsd3, and Lhr) related genes as well as clock genes (Rev-erbα, Bmal1, Per1, Per2, Cry1, and Cry2) in testes. Th...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-019-1626-y
更新日期:2019-11-01 00:00:00
abstract::Early brain injury (EBI), highlighted with inflammation and apoptosis, occurring within 72 h after subarachnoid hemorrhage (SAH), is associated with the prognosis of SAH. Recent studies have revealed that hydrogen-rich saline (HS) exerted multiple neuroprotective properties in many neurological diseases including SAH,...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-015-9242-y
更新日期:2016-07-01 00:00:00
abstract::Classical galactosemia is an inborn error of carbohydrate metabolism in which patients accumulate high concentration of galactose in the brain. The most common treatment is a galactose-restricted diet. However, even treated patients develop several complications. One of the most common symptoms is motor coordination i...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-016-9981-4
更新日期:2017-08-01 00:00:00
abstract::Docosahexaenoic acid (DHA) is enriched in membrane phospholipids of the central nervous system (CNS) and has a role in aging and neuropsychiatric disorders. DHA is metabolized by the enzyme Alox15 to 17S-hydroxy-DHA, which is then converted to 7S-hydroperoxy,17S-hydroxy-DHA by a 5-lipoxygenase, and thence via epoxy in...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-017-0413-x
更新日期:2018-02-01 00:00:00
abstract::Inhibition of potassium channels is possibly the first step in the sequence of biochemical events leading to memory formation. These channels appear to be regulated directly or indirectly by GTP-binding proteins (G proteins), which may themselves be affected by phosphorylation and dephosphorylation in response to elev...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/BF02935554
更新日期:1991-01-01 00:00:00
abstract::Amyloid-β oligomers (AβOs) toxicity causes mitochondrial dysfunction, leading to synaptic failure in Alzheimer's disease (AD). Considering presynaptic high energy demand and tight Ca2+ regulation, impairment of mitochondrial function can lead to deteriorated neural activity and cell death. In this study, an AD mouse m...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-020-02064-4
更新日期:2020-11-01 00:00:00
abstract::A recent genome-wide association study conducted in Caucasians has identified glutaminyl-peptide cyclotransferase (QPCT) gene as a susceptibility gene for schizophrenia, as its common single nucleotide polymorphism (SNP) rs2373000 was significantly associated with the risk of this disease. To date, this finding has no...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-015-9541-3
更新日期:2016-11-01 00:00:00
abstract::Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) and the BK channel are enriched at the presynaptic nerve terminal, where CaMKII associates with synaptic vesicles whereas the BK channel colocalizes with voltage-sensitive Ca(2+) channels in the plasma membrane. Mounting evidence suggests that these two proteins p...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-008-8039-7
更新日期:2008-10-01 00:00:00
abstract::The NMDA receptor, which is heavily involved in several human brain diseases, is a heteromeric ligand-gated ion channel that interacts with multiple intracellular proteins through the C-termini of different subunits. GluN2A and GluN2B are the two primary types of GluN2 subunits in the forebrain. During the development...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-016-9715-7
更新日期:2017-03-01 00:00:00