Abstract：
:The present study depicts the development of a validated RP-HPLC method for the determination of the pamabrom in presence of degradation products or other pharmaceutical excipients. Stress study was performed on pamabrom and it was found that it degrade sufficiently in acidic, alkali and oxidative condition but less degradation was found in thermal and photolytic condition. The separation was carried out on Enable G 120 A(0) (250×4.6 mm, 5 μ) column having particle size 5 μ using methanol: water (75:25 v/v) with pH 4.0 adjusted with ortho phosphoric acid as mobile phase at flow rate of 1 ml/min. The wavelength of the detection was 280nm. A retention time (Rt) nearly 3.9 min was observed. The calibration curve for pamabrom was linear (r (2) = 0.9997) from range of 10-60 μg/ml with limit of detection and limit of quantification of 1.41 μg/ml and 4.28 μg/ml, respectively. Analytical validation parameter such as selectivity, specificity, linearity, accuracy and precision were evaluated and relative standard deviation value for all the key parameters were less than 2.0%. The recovery of the drug after standard addition was found to be 101.35%. Thus, the developed RP-HPLC method was found to be suitable for the determination of pamabrom in bulk as well as stability samples of tablets containing various excipients.
journal_name
Indian J Pharm Scijournal_title
Indian journal of pharmaceutical sciencesauthors
Shah U,Kavad M,Raval Msubject
Has Abstractpub_date
2014-05-01 00:00:00pages
198-202issue
3eissn
0250-474Xissn
1998-3743journal_volume
76pub_type
杂志文章abstract：:It is now known that vitamin D deficiency is a worldwide health problem. In our country, as food fortification is lacking, supplementation with pharmaceutical preparations is the only means of treatment of vitamin D deficiency. We aimed to study the composition and availability of various vitamin D preparations in the...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474x.180248
更新日期：2016-01-01 00:00:00
abstract：:Piper claussenianum inflorescences crude methanol extract was tested for hypoglycemic effect in streptozotocin-induced diabetic rats. The blood glucose levels of rats treated with methanol extract were reduced from 318.4±28.1 mg/dl before treatment to 174.2±38.3 mg/dl after 12 days of treatment (P<0.05). Phytochemical...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474x.156624
更新日期：2015-03-01 00:00:00
abstract：:The petroleum ether leaf extract of Ficus krishnae has been evaluated for the management of diabetes in alloxan induced diabetic rats. Phytochemical screening of the leaf extract for various chemical compounds has also been carried out. Leaf extract was administered continuously for 21 days orally at a dose of 200 mg/...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：:The objective of this work was to increase the amount of acyclovir in the basal epidermis, site of herpes virus simplex infection, using the solid lipid nanoparticles loaded gel cream as carriers. Solid lipid nanoparticles were prepared by high pressure homogenisation method and incorporated in a semisolid submicron g...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：:Various (4-substituted) phenyl-3-β-[(N-benzenesulphonyl/tosyl)-4-(un)substituted anilino]propionylamido-1,3-thiazolidine-4-ones (3a-x) and 1-β-[(N-benzenesulphonyl/tosyl)-4-(un)substituted anilino]-propionylamido-3-chloro-4-(4-substituted)phenyl-azetidin-2-ones (4a-x) have been synthesised by the cyclocondensation of ...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.95654
更新日期：2011-07-01 00:00:00
abstract：:Alpinia officinarum is a rhizome belonging to the family zingeberaeceae. Hydro alcoholic extract by hot and cold maceration and methanol extract by percolation process Qualitative phytochemical analysis of extract of Alpinia officinarum rhizome showed a majority of the compound including tannins, alkaloids, flavonoids...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.62233
更新日期：2010-01-01 00:00:00
abstract：:Ethanol extract of Passiflora edulis Sims was analyzed for its antioxidant (1,1-diphenyl-2-picryl hydrazyl radical reducing power methods) and phytochemical analysis. The extract was found effective against the antioxidant test models exhibiting an IC(50) value of 875+/-87.83 mug/ml and showed strong potential antioxi...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.56038
更新日期：2009-05-01 00:00:00
abstract：:Crystal form can be crucial to the performance of a dosage form. This is especially true for compounds that have intrinsic barriers to drug delivery, such as low aqueous solubility, slow dissolution in gastrointestinal media, low permeability and first-pass metabolism. The nature of the physical form and formulation t...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.57283
更新日期：2009-07-01 00:00:00
abstract：:A novel, efficient and simple approach for soy phosphatidylcholine analysis according to its fatty acid composition was studied with reverse-phase high-performance liquid chromatography. The reverse-phase high-performance liquid chromatography analysis was performed isocratically using UV detector and simple mobile ph...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.117435
更新日期：2013-05-01 00:00:00
abstract：:A simple, precise, and accurate isocratic reversed-phase (RP) stability-indicating HPLC assay method was developed and validated for determination of Aripiprazole in bulk and solid pharmaceutical dosage form. A reversed-phase C8 (250×4.0 mm, 5 μm particle size) column for HPLC and C8 (50×2.1mm, 1.7 μm particle size) f...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.95638
更新日期：2011-07-01 00:00:00
abstract：:The effect of some physical and nutritional parameters were studied for the optimum production of extracellular enzyme hyaluronidase employing Streptococcus mitis MTCC*2695 by submerged fermentation. The effects of initial pH, incubation temperature and time, inoculum level and age of inoculum were studied. The maximu...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.45412
更新日期：2008-09-01 00:00:00
abstract：:This study aims at the isolation of filamentous fungi, extraction of metabolites, and evaluation of the cytotoxic properties on HeLa cells and normal human lymphocytes. We isolated fungi from the soil by serial dilution method. One of the isolates was chosen and identified as Aspergillus ochraceus Wilhelm (Trichocomac...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：
更新日期：2013-09-01 00:00:00
abstract：:A derivative UV spectrophotometric and a reversed phase high-performance liquid chromatographic method for the determination of ciprofibrate in tablets was developed. The first-order derivative UV spectrophotometric method was found to be accurate with 100.57±0.97 recovery and precise with a coefficient of variation o...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.103855
更新日期：2012-03-01 00:00:00
abstract：:Fexofenadine is a selective histamine H(1) receptor antagonist, used for relief of the symptoms of allergy. However its aqueous solubility is very poor. Solid inclusion complexes of fexofenadine and β-cyclodextrin were prepared at the molar ratios of 1:1 and 1:2 by kneading, and coprecipitation methods to improve its ...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.70477
更新日期：2010-05-01 00:00:00
abstract：:A stability-indicating reversed-phase liquid chromatographic method has been developed and validated for simultaneous determination of losartan potassium and ramipril. Separations were achieved using a C(18) column with mobile phase consisting of acetonitrile and (0.2% v/v, pH 2.5) aqueous trifluoroacetic acid (45:55,...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.106061
更新日期：2012-05-01 00:00:00
abstract：:Two UV Spectrophotometric and one reverse phase high performance liquid chromatography methods have been developed for the simultaneous estimation of amlodipine besilate and olmesartan medoxomil in tablet dosage form. First UV spectrophotometric method was a determination using the simultaneous equation method at 237....
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.58190
更新日期：2009-09-01 00:00:00
abstract：:Objective of the present study was to develop and evaluate vaginal films with essential in vitro studies. Films were developed using hydroxypropyl methylcellulose as a polymer and formulations were coded. The developed films were evaluated with Fourier transform infrared spectroscopy, drug content, viscosity, surface ...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：
更新日期：2013-09-01 00:00:00
abstract：:Several 4-arylidene-2-phenyl-1-(2,4,5-trichlorophenyl)-1H-imidazol-5(4H)-ones (4a-q), N-(4-benzylidene-5-oxo-2-phenyl-4,5-dihydroimidazol-1-yl)-4-chlorobenzamides (5a-o) and N-(4-benzylidene-5-oxo-2-phenyl-4,5-dihydroimidazol-1-yl)-2,4-dichlorobenzamides (6a-m) were prepared. All newly synthesized compounds have been ...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.51953
更新日期：2009-01-01 00:00:00
abstract：:Mitochondrial sodium calcium exchange inhibitors are novel agents in the treatment of type-II diabetes due to their glucose dependent efficacy. While the compounds of this class are expected to correct hyperglycemia, they do not lower basal blood glucose level, thus avoiding the serious consequences of hypoglycemia. T...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.41453
更新日期：2008-03-01 00:00:00
abstract：:The objective of this study was to prepare and characterise nevirapine nanosuspensions so as to improve the dissolution rate of nevirapine. Nevirapine is a nonnucleoside reverse transcriptase inhibitor of immunodeficiency virus type-1 and it is poorly water-soluble antiretroviral drug. The low solubility of nevirapine...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:The aim of the present study was the determination of formulation factors and the in vitro evaluation of an extended release dosage form of a freely soluble weakly basic drug (alfuzosin hydrochloride). Binary mixer of one hydrophilic polymer (hydroxypropylmethylcellulose) and one directly compressible Eudragit (RS PO)...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.56019
更新日期：2009-05-01 00:00:00
abstract：:Gastroretentive bimodal drug delivery systems of lamotrigine were developed using immediate release and extended release segments incorporated in a hydroxypropyl methylcellulose capsule and in vitro and in vivo evaluations were conducted. In vivo radiographic studies were carried out for the optimized formulation in h...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：
更新日期：2014-11-01 00:00:00
abstract：:A simple, rapid, reliable, robust and optimized reversed phase high performance liquid chromatographic method for simultaneous estimation of doxycycline hyclate and curcumin was successfully developed and validated as per International Conference on Harmonization guidelines. The objective was achieved in terms of well...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474x.174992
更新日期：2015-11-01 00:00:00
abstract：:A field experiment on the effect of time of harvesting on yield and quality of Melissa officinalis L. was conducted under the agroclimatic conditions of Doon valley, Uttarakhand in order to assess the performance of four harvesting times (H1-120 days, H2-140 days, H3-160 days and H4-180 days after planting). The fresh...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：:A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of atorvastatin calcium and fenofibrate in tablet formulation. The separation was achieved by Luna C18 column and methanol:acetate buffer pH 3.7 (82:18 v/v) as mobile phase, at a flow rate of 1.5 ml/min. Detect...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.41473
更新日期：2008-03-01 00:00:00
abstract：:A gradient reversed phase HPLC method was developed and validated for analysis of betamethasone dipropionate, its related substances and degradation products, using Altima C(18) column (250×4.6 mm, 5 μm) with a flow rate of 1.0 ml/min and detection wavelength of 240 nm. The mobile phase A is a mixture of water, tetrah...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.103840
更新日期：2012-03-01 00:00:00
abstract：:The present study is designed to evaluate the efficacy of heptoplus a polyherbal formulation as an oral supplementary agent for isoniazid and rifampicin induced hepatotoxicity in rats. 50 and 100 mg/kg of heptoplus supplement were fed orally to the rats along with isoniazid and rifampicin and compared to rats treated ...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474x.169028
更新日期：2015-09-01 00:00:00
abstract：:Bacteria have developed multidrug resistance against available antimicrobial agents. Infectious diseases caused by these multidrug-resistant bacteria are major causes of morbidity and mortality in human beings. Synthetic drugs are expensive and inadequate for the treatment of diseases, causing side effects and ineffec...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.174991
更新日期：2015-11-01 00:00:00
abstract：:Floating matrix tablets of domperidone were developed to prolong gastric residence time and thereby increased drug bioavailability. Domperidone was chosen as a model drug because it is poorly absorbed from the lower gastrointestinal tract. The tablets were prepared by wet granulation technique, using polymers such as ...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.51944
更新日期：2009-01-01 00:00:00
abstract：:Present study reports the development and validation of a simultaneous estimation of metformin and gliclazide in human plasma using supercritical fluid chromatography followed by tandem mass spectrometry. Acetonitrile:water (80:20) mixture was used as a mobile phase along with liquid CO(2) in supercritical fluid chrom...
journal_title：Indian journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.4103/0250-474X.62231
更新日期：2010-01-01 00:00:00
