Abstract:
:Young adult and old mice were immunized by footpad injection of dinitrophenyl-conjugated bovine gamma-globulin (DNP-BGG) in complete Freund's adjuvant. A comparison of lymph node weight and total number of nucleated cells per lymph node as a function of time after antigen injection demonstrated a significantly greater absolute increase in lymph node weight and peak number of nucleated cells per lymph node in young-adult than in old animals. However, as judged by this increase in total nucleated cells, other than being delayed in old mice, the magnitude of these in situ proliferative responses appeared comparable for young-adult and old mice. That is, the antigen-stimulated to non-stimulated cell ratio did not differ significantly between young-adult and old animals. This was because lymph nodes from old animals prior to antigen injection always weighed less and had fewer numbers of nucleated cells compared with young-adult animals. Therefore, the in vitro cellular proliferative response of three T-cell-enriched lymphocyte subpopulations from young-adult and old mice was further characterized. This was done by measuring [3H]thymidine incorporation following antigen- (DNP-BGG)- or mitogen-[phytohemagglutinin (PHA) or Concanavalin A (Con A)]-induced proliferation and assessing their quantitative and/or qualitative requirements for macrophages. In contrast to the markedly reduced proliferation of the two T-cell subpopulations from popliteal lymph nodes which respond to PHA and Con A in old animals primed 21-days earlier with DNP-BGG, antigen-induced in vitro cellular proliferation of the small T-cell subset in old mice specifically responsive to the immunizing antigen DNP-BGG always responded as well as, if not better than, cells from young-adult mice.
journal_name
Mech Ageing Devjournal_title
Mechanisms of ageing and developmentauthors
Perkins EH,Glover PLdoi
10.1016/0047-6374(83)90072-6subject
Has Abstractpub_date
1983-02-01 00:00:00pages
169-82issue
2eissn
0047-6374issn
1872-6216pii
0047-6374(83)90072-6journal_volume
21pub_type
杂志文章abstract::Morphological studies on spontaneous systemic amyloidosis were conducted on 222 senescence-accelerated mice (SAM) (P) and on 150 mice in the senescence-resistant series (R). Among the pathologic findings, amyloidosis showed the highest incidence in both SAM (79.7%) and R (32.7%). Although an extensive deposition of am...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(82)90070-7
更新日期:1982-09-01 00:00:00
abstract::The kidney glomeruli of 9-month-old intact, 23-month-old intact, and 23-month-old hypophysectomized female rats were examined by scanning and transmission electron microscopy. With increasing age, the glomeruli increased in diameter and more podocyte microvilli were found. Hypophysectomy (4 months before sacrifice) re...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(80)90130-x
更新日期:1980-05-01 00:00:00
abstract::The progression of many neurodegenerative diseases is assumed to be caused by misfolding of specific characteristic diseases related proteins, resulting in aggregation and fibril formation of these proteins. Protein misfolding associated age related diseases, although different in disease manifestations, share strikin...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/j.mad.2016.03.001
更新日期:2016-06-01 00:00:00
abstract::The human premature aging protein Werner (WRN), deficient in Werner syndrome (WS), is localized mainly to the nucleolus in many cell types. DNA damage or replication arrest causes WRN to redistribute from the nucleolus to the nucleoplasm into discrete foci. In this study, we have investigated DNA damage specific cellu...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2005.06.004
更新日期:2005-11-01 00:00:00
abstract::The purpose of this study was to determine the feasibility of using a recently developed procedure, dual-energy x-ray absorptiometry, to determine body composition in rats as a function of aging. Results were obtained that were consistent with previous findings for male rats. ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(93)90043-q
更新日期:1993-10-01 00:00:00
abstract::Human diploid fibroblasts, TIG-1, were serially subcultivated at low inoculation densities thus maintaining the cultures in log phase throughout their in vitro lifespan. Their lifespan was shortened in calendar time without decreasing the cumulative number of population doublings when compared with the lifespan of cel...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(80)90034-2
更新日期:1980-07-01 00:00:00
abstract::There is currently of a great interest investigating the role of nutrition in the prevention of age-associated disorders. The present study aimed to evaluate, on a particular strain of mice, the efficacy of alternate-day fasting on the mitochondrial production of free radical species and on the incidence of a specific...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2005.06.007
更新日期:2005-11-01 00:00:00
abstract::Hepatocyte morphology of the three zones of the liver lobule was evaluated by stereological analysis in 3-month-old and 35-month-old virgin female WAG/Rij rats. In the old rats, the volume of the nucleus and cytoplasm, and volume densities of smooth endoplasmic reticulum and lysosomes were increased in all areas of th...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(80)90054-8
更新日期:1980-06-01 00:00:00
abstract::The hypothesis that aging is associated with the accumulation of oxidative damage was tested in the adult male housefly by monitoring the loss of membrane protein -SH groups, and activities of glucose-6-phosphate dehydrogenase and alcohol dehydrogenase, the cytosolic enzymes that are particularly susceptible to oxidat...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(94)90024-8
更新日期:1994-07-01 00:00:00
abstract::Detailed structural, mutational, and biochemical analyses of human FEN1/DNA complexes have revealed the mechanism for recognition of 5' flaps formed during lagging strand replication and DNA repair. FEN1 processes 5' flaps through a previously unknown, but structurally elegant double-stranded (ds) recognition/single s...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/j.mad.2011.11.009
更新日期:2012-04-01 00:00:00
abstract::The B cell responsiveness mediated by T cells has been evaluated in 50 aged donors by using a Pokeweed mitogen (PWM)-driven differentiation system. Peripheral blood mononuclear cells (PBMC) from aged donors exhibited a lower plaque-forming cell (PFC) generation in comparison with young donors. Similar results were obt...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(87)90056-x
更新日期:1987-11-01 00:00:00
abstract::We have isolated a new type of Paramecium tetraurelia mutant, named rie-2, that has a long immaturity period until autogamy. We previously isolated such an autogamy mutant, designated rie-1. These two mutants had some additional common features such as dependence of the occurrence of autogamy on the temperature, invol...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2005.01.010
更新日期:2005-06-01 00:00:00
abstract::Quantitative measurements of glycogen in aging bruchids showed there was a considerable decrease (> 50%) with age except on the fifth day when an increase was observed, followed by a decrease on the sixth day. The variations in glycogen content with age suggests that glycogen serves as one of the energy sources respon...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(80)90082-2
更新日期:1980-01-01 00:00:00
abstract::Ascorbic acid content decreased with age in livers of male rats but not in those of female rats. The influence of aging on the regeneration of ascorbic acid from dehydroascorbic acid was investigated as a possible cause of declining ascorbate concentrations. The results demonstrate that hepatic NADPH-dehydroascorbic a...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(96)01784-8
更新日期:1996-11-13 00:00:00
abstract::The influence of age on liver gene expression was investigated in two strains of H-2 congenic mice. In B10.RIII mice (H-2r), basal P1- and P3-450 RNA levels progressively decreased 65 and 95%, respectively, between 4 and 28 months of age (P less than or equal to 0.05). Polyaromatic hydrocarbon (PAH) induced P1- and P3...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(90)90038-h
更新日期:1990-03-31 00:00:00
abstract::Functional defects that accumulate in the T cell compartment are thought to be responsible for the pronounced immunodeficiency that develops during ageing, and reduced production of T cells by the thymus as it undergoes involution has been suggested to contribute to this phenomenon. Understanding the mechanisms respon...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/s0047-6374(96)01818-0
更新日期:1997-02-01 00:00:00
abstract::Nitric oxide synthase (NOS) activity was enhanced in human umbilical vein endothelial cells (HUVECs) by the combined stimulation with IFN-gamma plus IL-1beta, TNF-alpha and LPS which was accompanied by cell death. DNA analysis of the NOS induced dead HUVECs showed that internucleosomal DNA fragmentation had occurred, ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(94)01579-b
更新日期:1995-06-30 00:00:00
abstract::We determined the number of fibroblastoid progenitors (fibroblastoid colony-forming units, CFU-F) in femurs and spleens derived from (CBA X C57BL)F1 mice of different ages. The femoral CFU-F population size increased from 350 at 1 week of age and plateaued at approximately 1900 CFU-F at 8 weeks of age. The mean incide...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(83)90003-9
更新日期:1983-05-01 00:00:00
abstract::Relationships between nutrition and longevity are of growing interest. Here we analysed the influences of dietary restriction on the survival of Drosophila exposed to atmospheric oxygen or to chronic hypoxia. Dietary restriction was achieved by food dilution, by sucrose restriction or by yeast restriction. Sucrose and...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2007.05.008
更新日期:2007-05-01 00:00:00
abstract::In order to gain a better understanding of tissue plasticity with aging, we investigated the adaptive responses of young and adult animals to both 7 and 28 days of hypobaric hypoxia. Senescence is associated with a decreased tolerance to hypoxia that may be related to an age-associated decline in glucose transporter s...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(01)00216-0
更新日期:2001-05-15 00:00:00
abstract::Following a 3 hour in vivo labelling of cytoplasmic RNA in rat liver with orotic acid-6-14C under conditions where ribosomal RNA synthesis was suppressed, the proportion of labelled messenger-like RNA released to the cytoplasm which contained polyadenylate (poly(A)) tracts was about 3.0 times higher in the livers of j...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(77)90026-4
更新日期:1977-07-01 00:00:00
abstract::Keratinocytes and fibroblasts were obtained from upper arm biopsies of young (22-27 years) and old (60-82 years) adult donors. Keratinocytes were grown in serum-free medium containing variable quantities of either epidermal growth factor (EGF) or a bovine hypothalamic extract known to contain keratinocyte growth facto...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(86)90009-6
更新日期:1986-07-01 00:00:00
abstract::The polyphenol quercetin has recently been found to extend lifespan and increase stress resistance in the nematode Caenorhabditis elegans. The forkhead transcription factor DAF-16 has previously been linked to these effects. However, by using a daf-16(mgDf50) mutant strain, we show that quercetin exposure leads to inc...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2008.07.001
更新日期:2008-10-01 00:00:00
abstract::The present study was designed to assess whether treatment with the monoamine oxidase-B (MAO-B) inhibitor L-deprenyl, which has been documented to increase both mean and maximum survival in aged rats as well as sexual performance and cognitive function, has any effect on the age-related microanatomical changes occurri...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(94)90060-4
更新日期:1994-02-01 00:00:00
abstract::Filopodia in log and stationary phase populations of human fetal lung fibroblasts (WI-38) at low and high population doubling levels (PDLs) and of SV40 transformed WI-38 cells (VA13A), were observed and counted under different conditions of in vitro growth by scanning electron microscopy. Cells from old non-vigorously...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(86)90048-5
更新日期:1986-02-01 00:00:00
abstract::Age-specific incidence rates for cancers of the female breast, ovary, corpus uteri, testis, prostate and male and female cancers of the stomach, colon, rectum, lung, and male and female Hodgkin's disease in 51 worldwide populations in the 1990 era were obtained from a published source. For each cancer in each populati...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(02)00113-6
更新日期:2003-03-01 00:00:00
abstract::Overproduction of oxidants and free radicals in ageing tissues induces nitro-oxidative stress, which has recently been implicated in the pathogenesis of cardiovascular dysfunction associated with ageing. Peroxynitrite, a strong cytotoxic oxidant damages proteins and DNA and activates several pathways causing tissue in...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2006.09.005
更新日期:2007-02-01 00:00:00
abstract::Age-adjusted mortality rates for breast cancer (BC) in the United States from 1962 to 1987 were subjected to longitudinal Gompertzian analysis. Age-adjusted BC mortality rate distributions for women display two distinct Gompertzian slopes. Between age 15 and 40 years, age-adjusted BC mortality rate distributions inter...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(91)90125-j
更新日期:1991-10-01 00:00:00
abstract::The bone marrow of old adult mice ( approximately 2 years old) has reduced B lymphopoiesis; however, whether the B1 pathway in adult bone marrow is also compromised in senescence is not known. Herein, we show that phenotypic (IgM(-)Lin(-)CD93(+)[AA4.1(+)] CD19(+)B220(low/-)) B1 progenitors are retained in old bone mar...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2009.04.001
更新日期:2009-06-01 00:00:00
abstract::Hepatocytes isolated from 6-, 12-, 18-, and 30-month-old female Fischer F344 rats were examined by scanning electron microscopy. No significant change in cell size with age was observed. However, the surface morphology of the cells isolated from the older animals exhibited a significant increase in surface folds. This...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(81)90023-3
更新日期:1981-01-01 00:00:00