Abstract:
:Previous reports suggest that 33 degrees C is the optimum temperature for the long-term culture of murine bone marrow in vitro. We found that horse serum was an important variable determining our ability to culture marrow from immature (less than 10 weeks) mice at 37 degrees C. While some batches supported growth others did not. The ability of deficient horse serum to support growth could be corrected if the culture was fed at mid-week with additional medium. Marrow from aged mice (96 weeks and older) could invariably be cultured at 37 degrees C irrespective of the horse serum batch used for culture. Adult mice (20-24 weeks) gave intermediate results between immature and aged mice. All three groups of mice studied could be cultured at 33 degrees C irrespective of the batch of horse serum used or the age of the donor. These results indicate that horse serum provides a factor(s) which varies in concentration from batch to batch and is essential for normal marrow growth in vitro. The requirement for this factor is greater at higher temperature and in younger animals. Marrow from adult and aged mice was then simultaneously cultured at 33 degrees C and 37 degrees C. For adult mice the cell recovery at 33 degrees C was initially significantly higher than at 37 degrees C but at later times became less. As a result cumulative recovery at both temperatures was equal. Cumulative cell recovery and culture survival time was significantly less in the aged than the young mice cultures.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Mech Ageing Devjournal_title
Mechanisms of ageing and developmentauthors
Lipschitz DA,Udupa KBdoi
10.1016/0047-6374(84)90179-9subject
Has Abstractpub_date
1984-01-01 00:00:00pages
119-27issue
1eissn
0047-6374issn
1872-6216pii
0047-6374(84)90179-9journal_volume
24pub_type
杂志文章abstract::Age-specific mortality rates from stomach cancer (SC) in Japan from 1950 through 1993 were subjected to longitudinal Gompertzian analysis. Age-specific SC mortality rate distributions between ages 45 and 75 years were highly Gompertzian for each- and every year for both men and women. Between 1950 and 1993, age-adjust...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(95)01669-4
更新日期:1995-11-24 00:00:00
abstract::Although myocardial actomyosin ATPase specific activity determined at Ca2+ levels of 1 x 10(-3) to 1 x 10(-2) M is consistently higher in two-month old male Fischer rats than in twelve-month old individuals, this difference can be eliminated if the enzyme is assayed at lower calcium concentrations. This suggests that ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(78)90040-4
更新日期:1978-12-01 00:00:00
abstract::Glycosaminoglycan (GAG) distribution has been analyzed in the adhesion sites, left substratum-bound after EGTA-mediated detachment, of various human skin fibroblast populations grown in vitro in the presence (asc+) or absence (asc-) of ascorbate. Examination of these skin fibroblasts during the EGTA treatment by scann...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(85)90015-6
更新日期:1985-02-01 00:00:00
abstract::T cells in vivo have been shown to accumulate DNA damage with age. To investigate the effects of DNA damage on T cell biology we have utilised an in vitro human CD4+ T cell clone model. Levels and types of DNA damage were determined in 11 independent T cell clones as a function of their in vitro lifespan. Increased le...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(01)00254-8
更新日期:2001-08-01 00:00:00
abstract::The circulating antibody titers to bovine serum albumin (BSA) were determined for two groups of aged Fischer 344 rats obtained form the National Institute on Aging. One group received a single immunization of BSA in complete Freund's adjuvant, and the second group was immunized three times at two-week intervals with t...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(81)90032-4
更新日期:1981-05-01 00:00:00
abstract::The various phases of homo- and heteroclonal encounters between stolons of Podocoryne carnea M. Sars have been studied with the light and electron microscope. The first clone-unspecific actions and reactions to an imminent encounter between the outgrowing tip of stolon and the subterminal part of an opposed homologous...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(83)90045-3
更新日期:1983-03-01 00:00:00
abstract::The hypothesis that aging is associated with the accumulation of oxidative damage was tested in the adult male housefly by monitoring the loss of membrane protein -SH groups, and activities of glucose-6-phosphate dehydrogenase and alcohol dehydrogenase, the cytosolic enzymes that are particularly susceptible to oxidat...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(94)90024-8
更新日期:1994-07-01 00:00:00
abstract::Scavenger receptor B1 (SR-B1) is a trans-membrane protein, involved in tissue reverse cholesterol transport. Several studies have demonstrated that SR-B1 is also implicated in other physiological processes, such as bacteria and apoptotic cells recognition and regulation of intracellular tocopherol and carotenoids leve...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2017.11.006
更新日期:2018-06-01 00:00:00
abstract::The tumor suppressors p16(INK4a) and p53 have been implicated as contributors to age-associated stem cell decline. Key functions of p53 are the induction of cell cycle arrest, senescence, or apoptosis in response to DNA damage. Here, we examine senescence, apoptosis, and DNA damage responses in a mouse accelerated agi...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2009.01.001
更新日期:2009-04-01 00:00:00
abstract::Normal diploid Syrian hamster dermal mesenchymal cell strains, regardless of the age of the tissue of origin, exhibit in vitro cellular senescence. The frequency of spontaneous escape from senescence and conversion to a permanent cell line is less than 5% among replicate flasks. The overall pattern of senescence of ce...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(86)90032-1
更新日期:1986-04-01 00:00:00
abstract::Aging causes changes in body composition and energy balance. Estrogen plays an important role in body's metabolism. The aim of this study was to determine whether estrogen has beneficial effects on leptin responsiveness in aged mice. Young 4 months and aged 19-21 female mice fed High Fat Diet (HFD) or Standard Diet (S...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2019.111198
更新日期:2020-03-01 00:00:00
abstract::In the present study, a number of physiological and behavioral measures that are related to metabolism were continuously monitored in 19-month-old male Fischer 344 rats that were fed ad libitum or fed a caloric restricted diet. Caloric restricted rats ate fewer meals but consumed more food during each meal and spent m...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/0047-6374(89)90044-4
更新日期:1989-05-01 00:00:00
abstract::We tested the hypothesis that Ca(2+)-activated myosin ATPase activity is lower in muscles of aged rats relative to muscles of young rats, independent of changes in myosin isoform expression. Myofibrils were prepared from permeabilized fibers of soleus, plantaris, and semimembranosus muscles of young (8-12 months) and ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2004.07.002
更新日期:2004-09-01 00:00:00
abstract::Highly purified DNA has been prepared from human muscle tissue and treated with three different single-strand specific desoxyribonucleases. The molecular weight of treated individual DNA molecules was determined by length measurements. Average DNA molecular weights and their standard deviations were correlated to the ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(83)90089-1
更新日期:1983-07-01 00:00:00
abstract::Antioxidants and telomere length are potential biomarkers for individuals' exposure and ability to cope with environmental stressors. However, intraspecific variations in antioxidant alterations due to natural, life cycle related stress, have been rarely estimated. We investigated those changes in wild-derived house m...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2017.10.002
更新日期:2018-01-01 00:00:00
abstract::Gut microbiota is among the factors that may be involved in healthy aging. Broader and geographically spread studies on gut microbiota of centenarians can help in identifying a common signature of longevity. We identified an endogamous Indian population with high centenarian prevalence. Here, we compared the gut micro...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2019.02.001
更新日期:2019-04-01 00:00:00
abstract::Menopause is characterized by the depletion of estrogen that has been proposed to cause oxidative stress. Circadian rhythm is an internal biological clock that controls physiological processes. It was analyzed the gene expression in peripheral blood mononuclear cells and the lipids and glucose levels in plasma of a su...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.mad.2017.04.002
更新日期:2017-06-01 00:00:00
abstract::An increase in natural (innate) immunity with age (through 65 weeks) of NBR rats to a syngeneic methylcholanthrene-induced fibrosarcoma (MCA) is described. This increase is characterized by the appearance of spontaneous tumor regressor animals and an increase in the incidence of tumor non-takes by rats 45 weeks of age...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(81)90129-9
更新日期:1981-09-01 00:00:00
abstract::Chronic oxidative stress is generally believed to be a major etiologic factor in the aging process. In addition to modulation of signaling processes and oxidation of cellular proteins and lipids, reactive oxygen species (ROS) induce multiple damages in both nuclear and mitochondrial genomes, most of which are repaired...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2004.07.005
更新日期:2004-10-01 00:00:00
abstract::The antigen-specific antibody secretion in vitro after immunisation with the primary T-cell dependent antigen Helix pomatia Haemocyanin (HPH) was investigated in both young and elderly individuals, who all met the health admission criteria for immunogerontological studies as detailed in the SENIEUR protocol. In additi...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(92)90069-p
更新日期:1992-01-01 00:00:00
abstract::The healing of normal incisional wounds and ischemic flap wounds was investigated in young (10 weeks) and old (102-104 weeks) rats, together with the effect of treatment with hyperbaric oxygen on day 0-3 of healing. After 10 days of healing all biomechanical strength parameters of normal wounds were decreased by 30-40...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(96)01782-4
更新日期:1996-10-25 00:00:00
abstract::Researchers and clinicians interested in human diseases of DNA repair deficiency and premature aging gathered at the National Conference Center in Lansdowne, Virginia on 5-8 September 2006 to attend a workshop co-organized by Vilhelm Bohr (National Institute of Aging) and Kenneth Kraemer (National Cancer Institute). A...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2006.11.028
更新日期:2007-02-01 00:00:00
abstract::In this paper, we approach the problem of attempting to understand senescence by understanding the complexity and hierarchical structure of mammalian organisms. To do this, we make use of some concepts from abstract relational biology; in particular, graph theory. We subsequently utilize these concepts to develop the ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(84)90056-3
更新日期:1984-10-31 00:00:00
abstract:BACKGROUND:It seems to be clear that hepatic age-related HMG-CoA reductase total activation is connected to a rise of reactive oxygen species (ROS). However, the mechanism by which ROS achieve this effect is unknown. Thus, in this work, we have performed a study of HMG-CoAR by analyzing the enzymes involved in its shor...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2007.10.001
更新日期:2007-11-01 00:00:00
abstract::Expression of dystrophin and the dystrophin-related protein utrophin has been studied in the human fetal brain both in vivo and in vitro. Results showed that both these proteins were developmentally regulated, even if their expression followed a different pattern. Utrophin was found since very early stages of developm...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(01)00360-8
更新日期:2002-03-15 00:00:00
abstract::In this article we review the abnormalities in immune function which have been described in relation to ageing. It is suggested that thymic involution may not be the only underlying cause. Many similar changes can be induced by the endotoxins from the cell walls of Gram-negative bacteria. The colon is a large reposito...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/0047-6374(84)90091-5
更新日期:1984-08-01 00:00:00
abstract::The decline in phytohemagglutinin (PHA) responsiveness of spleen cells from aged mice was studied in an effort to determine the nature of the depressed PHA reactivity. The reduced response of DNA synthesis (thymidine incorporation) to PHA stimulation could not be attributed to a decreased viability of spleen lymphocyt...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(75)90005-6
更新日期:1975-01-01 00:00:00
abstract::The effects of interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-alpha), and lipopolysaccharide (LPS) on the productions of nitric oxide (NO) and cGMP by cultured articular chondrocyte (AC) monolayers from 1-, 8- and 18-month old male Wistar rats were studied. It was found that basal NO and cGMP productions decre...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(97)00150-4
更新日期:1998-03-16 00:00:00
abstract::The peripheral-type benzodiazepine receptor (PBR) is a protein predominantly located in the mitochondrial outer membrane that plays an important role in the regulation of cell survival and proliferation. Previous studies have shown an enhanced expression of PBR in the regenerating sciatic nerve, suggesting that this p...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2005.06.001
更新日期:2005-11-01 00:00:00
abstract::The risk for developing colorectal cancer increases exponentially with age. We demonstrate that spontaneous loss of folate in the colon results in DNA damage accumulation and aberrant DNA damage responses that may contribute to the increased genomic instability and cancer risk in colon. We find greater than 2-fold cha...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2012.01.003
更新日期:2012-02-01 00:00:00