Abstract：
:Acute gene inactivation using short hairpin RNA (shRNA, knockdown) in developing brain is a powerful technique to study genetic function; however, discrepancies between knockdown and knockout murine phenotypes have left unanswered questions. For example, doublecortin (Dcx) knockdown but not knockout shows a neocortical neuronal migration phenotype. Here we report that in utero electroporation of shRNA, but not siRNA or miRNA, to Dcx demonstrates a migration phenotype in Dcx knockouts akin to the effect in wild-type mice, suggesting shRNA-mediated off-target toxicity. This effect was not limited to Dcx, as it was observed in Dclk1 knockouts, as well as with a fraction of scrambled shRNAs, suggesting a sequence-dependent but not sequence-specific effect. Profiling RNAs from electroporated cells showed a defect in endogenous let7 miRNA levels, and disruption of let7 or Dicer recapitulated the migration defect. The results suggest that shRNA-mediated knockdown can produce untoward migration effects by altering endogenous miRNA pathways.
journal_name
Neuronjournal_title
Neuronauthors
Baek ST,Kerjan G,Bielas SL,Lee JE,Fenstermaker AG,Novarino G,Gleeson JGdoi
10.1016/j.neuron.2014.04.036subject
Has Abstractpub_date
2014-06-18 00:00:00pages
1255-1262issue
6eissn
0896-6273issn
1097-4199journal_volume
82pub_type
杂志文章相关文献
NEURON文献大全abstract：:Changes in intracellular calcium (Ca) concentration following synaptic and suprathreshold activity are mediated by a wide range of sources and contribute to the regulation of myriad neuronal functions. The development of Ca imaging techniques has dramatically increased our understanding of the complex interactions bet...
journal_title：Neuron
pub_type： 杂志文章,评审
doi：10.1016/j.neuron.2008.08.020
更新日期：2008-09-25 00:00:00
abstract：:The synaptic vesicle protein synaptotagmin I binds Ca2+ and is required for efficient neurotransmitter release. Here, we measure the response time of the C2 domains of synaptotagmin to determine whether synaptotagmin is fast enough to function as a Ca2+ sensor for rapid exocytosis. We report that synaptotagmin is "tun...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/s0896-6273(00)80850-8
更新日期：1999-10-01 00:00:00
abstract：:Pathological aggregation of the microtubule-associated protein tau is a common feature of many neurodegenerative diseases. Although tau aggregation is associated with abnormal tau phosphorylation, the role of phosphorylation in the initiation of neurodegeneration has been unclear. Now, several animal models and data f...
journal_title：Neuron
pub_type： 杂志文章,评审
doi：10.1016/s0896-6273(03)00681-0
更新日期：2003-10-30 00:00:00
abstract：:Olfactory receptor neurons (ORNs) transform scant chemical inputs into significant neural signals. This transformation requires signal amplification. In this issue of Neuron, Ng et al. (2019) identified a mechanism by which the signals evoked by pheromones are amplified in the ORNs that selectively promote courtship b...
journal_title：Neuron
pub_type： 评论,杂志文章
doi：10.1016/j.neuron.2019.11.021
更新日期：2019-12-04 00:00:00
abstract：:Tau is abundant in the axon, whereas MAP2 is found in the cell body and dendrites. To understand their differential localization, we performed transfection studies on primary cultured neurons using tagged tau, MAP2, MAP2C, and their chimeric/deletion mutants. We found that MAP2 was prevented from entering the axon by ...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/0896-6273(95)90298-8
更新日期：1995-02-01 00:00:00
abstract：:Neuronal development is characterized by a period of exuberant synaptic growth that is well studied. However, the mechanisms that restrict this process are less clear. Here we demonstrate that glycosylphosphatidylinositol-anchored cell-surface receptors of the Nogo Receptor family (NgR1, NgR2, and NgR3) restrict excit...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/j.neuron.2011.11.029
更新日期：2012-02-09 00:00:00
abstract：:Effective mental functioning requires that cognition be protected from emotional conflict due to interference by task-irrelevant emotionally salient stimuli. The neural mechanisms by which the brain detects and resolves emotional conflict are still largely unknown, however. Drawing on the classic Stroop conflict task,...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/j.neuron.2006.07.029
更新日期：2006-09-21 00:00:00
abstract：:This study explores the nature of population coding in sensory cortex by applying information theoretic analyses to neuron pairs recorded simultaneously from rat barrel cortex. We quantified the roles of individual spikes and spike patterns in encoding whisker stimulus location. 82%-85% of the total information was co...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/s0896-6273(01)00481-0
更新日期：2001-11-08 00:00:00
abstract：:Advances in defining mechanisms of cortical development have been paralleled in recent years by an intense interest in translating these findings into greater insight of both childhood- and adult-onset cognitive and mental health disorders of developmental etiology. Successful integration of basic and clinical finding...
journal_title：Neuron
pub_type： 杂志文章,评审
doi：10.1016/j.neuron.2005.04.015
更新日期：2005-05-05 00:00:00
abstract：:Both the sec6/8 complex and septin filaments have been implicated in directing vesicles and proteins to sites of active membrane addition in yeast. The rat brain sec6/8 complex coimmunoprecipitates with a filament composed of four mammalian septins, suggesting an interaction between these complexes. One of the septins...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/s0896-6273(00)80493-6
更新日期：1998-06-01 00:00:00
abstract：:Immortalized retinal neurons have been established in tissue culture from retinal tumors arising in transgenic mice. The mice carry the SV40 T-antigen under the control of 5' flanking sequences from the human phenylethanolamine N-methyltransferase (PNMT) gene in order to target oncogene expression to adrenergic cell t...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/0896-6273(90)90204-s
更新日期：1990-05-01 00:00:00
abstract：:Our understanding of the mechanisms whereby BACE1, the aspartyl protease required for the initial cleavage of APP to generate amyloid-beta (Abeta), is regulated in Alzheimer's disease (AD) remains incomplete. In this issue of Neuron, O'Connor and coworkers show how energy deprivation, a potential risk factor in AD, tr...
journal_title：Neuron
pub_type： 评论,杂志文章
doi：10.1016/j.neuron.2008.12.010
更新日期：2008-12-26 00:00:00
abstract：:We describe a reaggregated cell culture system in which retinal neuroepithelial cells from embryonic rats proliferate extensively and give rise to rod photoreceptors on the same schedule in vitro as they do in vivo. Both the proliferative potential of the embryonic neuroepithelial cells and the timing of their differe...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/0896-6273(90)90058-n
更新日期：1990-03-01 00:00:00
abstract：:Experience is a powerful sculptor of developing neural connections. In the primary visual cortex (V1), cortical connections are particularly susceptible to the effects of sensory manipulation during a postnatal critical period. At the molecular level, this activity-dependent plasticity requires the transformation of s...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/s0896-6273(02)00966-2
更新日期：2002-10-24 00:00:00
abstract：:Seizure causes a rapid and protracted increase in transcription factor AP-1 levels in the brain. The composition of AP-1 nucleoprotein complexes changes with time after seizure as a result of the sequential appearance and disappearance of Fos and several Fos-related proteins. Although these changes occur over an 8 hr ...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/0896-6273(89)90260-2
更新日期：1989-09-01 00:00:00
abstract：:Protein scaffolds are essential for specific and efficient downstream signaling at synapses. Though nicotinic receptors are widely expressed in the nervous system and influence numerous cellular events due in part to their calcium permeability, no scaffolds have yet been identified for the receptors in neurons. Here w...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/s0896-6273(03)00324-6
更新日期：2003-06-05 00:00:00
abstract：:Brain nicotinic acetylcholine receptors (nAChRs) are made up of protein subunits that differ from those constituting muscle nAChRs. To characterize the physiological properties of one class of avian brain nicotinic receptor, we injected the nuclei of Xenopus oocytes with full-length cDNAs for the ligand binding (alpha...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/0896-6273(88)90132-8
更新日期：1988-11-01 00:00:00
abstract：:Visual experience before eye-opening is not usually thought to have any developmental significance. Here we show that naturalistic visual stimuli presented through unopened eyelids robustly activate neurons in the ferret dorsal lateral geniculate nucleus. Further, dark-rearing prior to natural eye-opening has striking...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/s0896-6273(02)01010-3
更新日期：2002-12-05 00:00:00
abstract：:We have examined the influence of retinal innervation on the development of target neurons in the first optic ganglion, the lamina, of D. melanogaster. Mitotically active lamina precursor cells (LPCs), which normally produce lamina neurons, are absent in mutants that lack retinal innervation, while other proliferative...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/0896-6273(91)90124-i
更新日期：1991-01-01 00:00:00
abstract：:Neurons adapt their synaptic responses to the activity of the underlying network. In this issue of Neuron, Thiagarajan and colleagues report on specific subcellular mechanisms of homeostasis after prolonged neuronal inactivity. The results have important implications not only for neuronal homeostasis but also for furt...
journal_title：Neuron
pub_type： 评论,杂志文章
doi：10.1016/j.neuron.2005.08.018
更新日期：2005-09-01 00:00:00
abstract：:The combination of patch-clamp and molecular biology techniques has made it possible to characterize the pharmacological and biophysical properties of ion channels in single neurons and to screen for expression of specific mRNAs in the same cell. Following whole-cell recording, the cytoplasm of the cell is harvested, ...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/0896-6273(95)90257-0
更新日期：1995-06-01 00:00:00
abstract：:Application of Clostridium difficile toxin B, an inhibitor of the Rho family of GTPases, at the Aplysia sensory to motor neuron synapse blocks long-term facilitation and the associated growth of new sensory neuron varicosities induced by repeated pulses of serotonin (5-HT). We have isolated cDNAs encoding Aplysia Rho,...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/j.neuron.2005.01.044
更新日期：2005-03-24 00:00:00
abstract：:During the development of forebrain connectivity, ascending thalamocortical and descending corticofugal axons first intermingle at the pallial-subpallial boundary to form the internal capsule (IC). However, the identity of molecular cues that guide these axons remains largely unknown. Here, we show that the transmembr...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/j.neuron.2014.05.020
更新日期：2014-07-02 00:00:00
abstract：:Neurotropic viruses that conditionally infect or replicate in molecularly defined neuronal subpopulations, and then spread transsynaptically, are powerful tools for mapping neural pathways. Genetically targetable retrograde transsynaptic tracer viruses are available to map the inputs to specific neuronal subpopulation...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/j.neuron.2011.12.002
更新日期：2011-12-22 00:00:00
abstract：:Inhibitory neurons, which play a critical role in decision-making models, are often simplified as a single pool of non-selective neurons lacking connection specificity. This assumption is supported by observations in the primary visual cortex: inhibitory neurons are broadly tuned in vivo and show non-specific connecti...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/j.neuron.2019.09.045
更新日期：2020-01-08 00:00:00
abstract：:The link between dysregulated serotonergic activity and depression and anxiety disorders is well established, yet the molecular mechanisms underlying these psychopathologies are not fully understood. Here, we explore the role of microRNAs in regulating serotonergic (5HT) neuron activity. To this end, we determined the...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/j.neuron.2014.05.042
更新日期：2014-07-16 00:00:00
abstract：:Neurotrophins and tyrosine receptor kinase (Trk) receptors are expressed in skeletal muscle, but it is unclear what functional role Trk-mediated signaling plays during postnatal life. Full-length TrkB (trkB.FL) as well as truncated TrkB (trkB.t1) were found to be localized primarily to the postsynaptic acetylcholine r...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/s0896-6273(00)81113-7
更新日期：1999-11-01 00:00:00
abstract：:The trigeminal loop in the brainstem comprises the innermost level of sensorimotor feedback in the rat vibrissa system. Anatomy suggests that this loop relays tactile information from the vibrissae to the motoneurons that control vibrissa movement. We demonstrate, using in vitro and in vivo recordings, that the trigem...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/j.neuron.2004.12.042
更新日期：2005-02-03 00:00:00
abstract：:Cell proliferation during successive stages of oligodendrocyte development was delineated in the rat brain and optic nerve. Surface antigens, A2B5, O4, and galactocerebroside (GalC) identified three cell populations emerging in sequence; the incorporation of bromodeoxyuridine into newly synthesized DNA identified the ...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/0896-6273(90)90216-3
更新日期：1990-11-01 00:00:00
abstract：:Spine morphology is regulated by intracellular signals, like PKC, that affect cytoskeletal and membrane dynamics. We investigated the role of MARCKS (myristoylated, alanine-rich C-kinase substrate) in dendrites of 3-week-old hippocampal cultures. MARCKS associates with membranes via the combined action of myristoylati...
journal_title：Neuron
pub_type： 杂志文章
doi：10.1016/j.neuron.2005.08.027
更新日期：2005-10-06 00:00:00
