Abstract:
:Chromatin organization was examined in confluent cultures of embryonic lung fibroblasts in the middle and at the end of their replicative lifespan. Age-related modifications resulted in an unusually wide spacing of the chromatin threads induced by a mild loosening treatment and an increased sensitivity of the nucleosomes to the Joy-containing hypotonic medium used in Miller's spreads. The profound changes in chromatin response to various hypotonic solutions were not associated with the disappearance of RNA synthesis in old type nuclei as demonstrated by high-resolution autoradiographical studies.
journal_name
Life Scijournal_title
Life sciencesauthors
Puvion-Dutilleul F,Puvion E,Macieira-Coelho Adoi
10.1016/0024-3205(83)90396-xsubject
Has Abstractpub_date
1983-06-13 00:00:00pages
2755-61issue
24eissn
0024-3205issn
1879-0631pii
0024-3205(83)90396-Xjournal_volume
32pub_type
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