Chromatin organization and transcriptional activity in human embryonic lung fibroblasts aged in vitro.

Abstract:

:Chromatin organization was examined in confluent cultures of embryonic lung fibroblasts in the middle and at the end of their replicative lifespan. Age-related modifications resulted in an unusually wide spacing of the chromatin threads induced by a mild loosening treatment and an increased sensitivity of the nucleosomes to the Joy-containing hypotonic medium used in Miller's spreads. The profound changes in chromatin response to various hypotonic solutions were not associated with the disappearance of RNA synthesis in old type nuclei as demonstrated by high-resolution autoradiographical studies.

journal_name

Life Sci

journal_title

Life sciences

authors

Puvion-Dutilleul F,Puvion E,Macieira-Coelho A

doi

10.1016/0024-3205(83)90396-x

subject

Has Abstract

pub_date

1983-06-13 00:00:00

pages

2755-61

issue

24

eissn

0024-3205

issn

1879-0631

pii

0024-3205(83)90396-X

journal_volume

32

pub_type

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