Abstract：
:Sustained chronic inflammation induces activation of genes involved in cellular proliferation and apoptosis, thereby causing skeletal muscle degeneration. To investigate in vitro effects of isolated pentacyclic triterpenes from Eugenia punicifolia (Ep-CM) upon signaling pathways involved in the regulation of skeletal muscle cell line proliferation, and in vivo muscular tissue remodeling. C2C12 cells were seeded on eight-well plates and [(3)H]-thymidine incorporation, TUNEL assays, mitochondria viability, zymography for matrix metalloproteases (MMPs), Western blot analysis for MAPKinase signaling pathway, NFκB activation and HMGB1 production subsequently determined under basal conditions and after Ep-CM treatment. A polymer containing Ep-CM was implanted on the volar surface of gastrocnemius muscles subjected to acute injury induced by bupivacaine for local slow and gradual release of bioactive compounds, and mice killed 4 days after surgery. Ep-CM inhibited proliferation of C2C12 myoblast cell line in a dose-dependent manner, confirmed by reduction of [(3)H]-thymidine uptake without affecting cell viability or inducing apoptosis. The cytostatic effect of Ep-CM occurred mainly via inhibition of phosphorylated extracellular signal-regulated kinase (pERK) activation and DNA synthesis, possibly inhibiting the G1 phase of the cell cycle, since Ep-CM increased pAkt and p27(kip1) but reduced Cyclin D1. Ep-CM in vitro treatment increased MMP-9 and MMP-2 activities of C2C12 myoblast cells, but reduced in vivo MMP-9 activity and acute muscular inflammation. Besides cytostatic and anti-inflammatory effects, Ep-CM pentacyclic triterpenes also contributed to degradation of basement membrane components by activating mechanisms of skeletal muscle remodeling in response to local injury.
journal_name
Arch Immunol Ther Exp (Warsz)journal_title
Archivum immunologiae et therapiae experimentalisauthors
Leite PE,Lima-Araújo KG,França GR,Lagrota-Candido J,Santos WC,Quirico-Santos Tdoi
10.1007/s00005-014-0291-0subject
Has Abstractpub_date
2014-12-01 00:00:00pages
483-91issue
6eissn
0004-069Xissn
1661-4917journal_volume
62pub_type
杂志文章abstract：:In 55 children in the acute phase of viral hepatitis with HB Ag antigenemia, and in 24 without antigenemia, total urinary protein excretion and frequency of excretion of the following serum proteins in urine were studied: alpha1-glycoprotein, haptoglobin, transferrin, A, G and M immunoglobulins, light immunoglobulin c...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:Identification of cells producing ATL was carried out on suspensions of cells enriched with lymphocytes T, B and macrophages. The cells were isolated from the spleen of syphilitic rabbits and from their testes when the rabbits were infected intratesticularly. The isolation of lymphocytes and macrophages was performed ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Nicotinic acetylcholine receptors (nAChRs) are membrane ligand-gated cation channels whose activation is triggered by the binding of the endogenous neurotransmitter acetylcholine or other biologic compounds including nicotine. Their roles in synaptic transmission in the central and peripheral nervous system as well as...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：10.1007/s00005-013-0266-6
更新日期：2014-04-01 00:00:00
abstract：:Recently, the role of small heat shock proteins (HSPs) has been widely recognized in cancer research. Small HSPs are tumorprotective via numerous, independent mechanisms such as: oxidative stress, protection preventing protein denaturation, anti-apoptotic activity, and likely direct suppression of the immune system. H...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：10.1007/s00005-006-0013-3
更新日期：2006-03-01 00:00:00
abstract：:Distribution of cytochrome P450 2C19 enzyme gene (CYP2C19) variants affecting metabolism of clopidogrel was determined in 526 Czech patients after percutaneous coronary intervention using MassARRAY genotyping and compared to distribution in other populations of European descent. Fifty-three (10%) patients underwent pa...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：10.1007/s00005-016-0440-8
更新日期：2016-12-01 00:00:00
abstract：:alpha-Carboline derivatives substituted with methyl groups at C-2, C-4, C-2,4, C-6 and C-8 position were subjected to N-1 methylation performed by Kitasatosporia setae. The yield of formed corresponding alpha-iso-carbolines varied, depending on the position of methyl substituent. It has been observed that the methyl g...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC) are the main autoimmune cholestatic liver diseases. IgG4-associated sclerosing cholangitis is another distinct immune-mediated cholestatic disorder of unknown aetiology that is frequently associated with autoimmune pancreatitis or other IgG4-re...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：10.1007/s00005-019-00550-9
更新日期：2019-08-01 00:00:00
abstract：:The bases for an efficient anti-tumor immune response begin to be better defined. Nonetheless, neoplastic cells develop various strategies to escape immune surveillance, which are discussed here in order to better design the therapeutic possibilities of immune manipulation. The absence of specific tumor antigen as wel...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：1999-01-01 00:00:00
abstract：:/We have studied the effect of interferon alpha (IFN-alpha) on MHC class II expression by human buccal epithelial cells (BEC), and mRNA expression by BEC and mucosal-associated mononuclear cells (MAMC). In 6 experiments, freshly collected BEC were suspended at a concentration of 1.0 x 10(5)/ml in RPMI 1640 and incubat...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:The aim of the study was to evaluate the sensitivity and specificity of allergen-induced basophil CD164 upregulation in patients with seasonal allergic rhinitis caused by allergy to grass pollens. This study was performed in 24 patients with allergy to grass pollens, and in 25 healthy controls. The protocol for allerg...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：10.1007/s00005-010-0104-z
更新日期：2010-12-01 00:00:00
abstract：:The production of sheep Pseudomonas immune serum is described. The animals were immunized with Pseudomonas vaccine prepared from strains of Pseudomonas aeruginosa belonging to seven immunotypes of Fisher's schema. Immune sera obtained from hyperimmunized sheep are characterized by a high level of stabel antibodies wit...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:A simple classification of cytokines is presented. It is based on voluminous information available from published papers of many outstanding researchers of the cytokines and their receptors and/or summarized by experts in this field. The cytokines are divided into 7 families according to their receptor code. Such arra...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：1997-01-01 00:00:00
abstract：:Clinical trials have shown that the type I interferon (IFN)-alpha/beta have some beneficial effects on organ-specific autoimmune diseases, such as Behcet's diseases and multiple sclerosis, although the precise mechanisms remain largely unresolved. T helper cells 1 (Th1)-mediated autoimmune responses are involved in th...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：2002-01-01 00:00:00
abstract：:When activated by a variety of stimuli, human neutrophils become capable of lysing tumour cells. The main physiologic cytotoxic trigger in neutrophils is represented by the surface receptor for the Fc domain of IgG (FcR). This receptor confers specificity to the target recognition by neutrophils and cooperates with ad...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：1992-01-01 00:00:00
abstract：:When BALB/c mice were first infected with Rauscher murine leukemia virus (MuLV-R) and then superinfected with Herpes simplex virus type 2 (HSV-2) the inhibition of the evolution of splenomegaly was observed. The effect did not depend on the route of HSV-2 administration. Experiments in which potent anti-interferon ser...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
abstract：:In this work the biocompatibility of porous bioceramic implanted to the rabbit femoral bone was studied. The animals were killed 3, 6, 9, 14, 18 and 30 days after implantation and the callus with surrounding periosteum from the site of implant was taken for the studies. Morphological investigations of the callus were ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:RNA was labeled with iodine in the reaction catalyzed with lactoperoxidase (LP). Labeling of RNA requires additional purification of LP on Sephadex G-100 and application of RNase inhibitors. Immobilized LP shows lower activity than a soluble enzyme but still it is sufficient to label RNA to 7% IC. Immobilized enzyme i...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:Preclinical pharmacological studies of two acridine derivatives, dihydrochloride N10-oxide 1-nitro-9-/3-dimethylaminopropylamino/-acridine (C-666) and dihydrochloride 1-nitro-9-[(2-dimethylamino)-1-methylethylamino]-acridine (C-829) are reported. Both compounds are characterized by biological activity, poor absorption...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:Mast cells (MCs) are found widely distributed in tissues and contribute to regulation of inflammatory responses and ongoing modulation of the tissues. Although MCs are important in a variety of processes, including innate immunity, their role in allergic disease has received increasing attention in the past decade. MC...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：2003-01-01 00:00:00
abstract：:The influence of antibiotics and purine and pyrimidine analogues on cells forming rosettes EA and EAC was evaluated in vitro. At the same time pH of solutions of examined immunosuppressors was estimated and a series of experiments with a buffer of appropriate pH was carried out. It was estimated that the effect inhibi...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
abstract：:During interferon induction in mice in vivo with various strains of influenza A virus differences in titer of produced interferon were observed. Correlation between interferon titer and neuraminidase activity of a given influenza virus strain was noted. Similar correlation was demonstrated with virus fraction purified...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:A mouse monoclonal IgA antibody (A008), anti-blood group A antigen, was shown to dissociate into heavy (H) and light (L) polypeptide chains under non-reducing conditions. The dissociation occurred in the presence of 0.2% sodium dodecyl sulphate (2 h at room temperature) or at elevated temperature (1 h at 70 degrees C)...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Pharmacological activity of 6 newly synthetized water-soluble derivatives of barbiturylo propanosulfonic acids, was evaluated. The compounds were obtained under the conditions of the Ritter reaction. The experiments were performed with the aim to determine the anti-inflammatory and immunotropic activities as well as t...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:Spotted souslik's interferon beta (So IFN beta), bovine interferon beta (Bov IFN beta) and mouse IFN beta/a were purified on Controlled Pore Glass (CPG) using original CPG, not modified, and CPG with boron enriched surface. The surface boron atoms enrichment of CPG lead to higher selectivity of interferon binding and ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Cytomegalovirus (CMV) is a persistent DNA virus that has evolved with humans to establish a finely balanced host-virus relationship. This balance is maintained by host immune surveillance since deficiencies in these processes can result in life-threatening disease, as observed in immunologically immature neonates and ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：10.1007/s00005-007-0037-3
更新日期：2007-09-01 00:00:00
abstract：:The effect of mice tissue homogenates on the inhibition of granulocyte-macrophage colony formation in vitro was studied. The potent inhibitory activity was displayed by homogenate from liver. The lowest values were found in the kidney and heart. An interrelationship between the rate of inhibition and protein concentra...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:By employing the rosette technique the peripheral T and B lymphocytes were determined in 24 patients with terminal renal failure treated by diet and 17 subjects treated by program of repeated hemodialysis. The decreased percentage of B-cells was found in both groups of patients in comparison with the control group. Th...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:Studies performed on chimpanzees and humans have revealed that strong, multispecific and sustained CD4(+) and CD8(+) T cell immune responses is a major determinant of hepatitis C virus (HCV) clearance. However, spontaneous elimination of the virus occurs in minority of infected individuals and cellular response direct...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：10.1007/s00005-015-0364-8
更新日期：2016-04-01 00:00:00
abstract：:Juvenile idiopathic arthritis (JIA) is the most common cause of chronic arthritis in childhood. Up to 50% of patients are resistant to standard therapy, which includes non-steroid anti-inflammatory drugs, corticosteroids, disease-modifying anti-rheumatic drugs and biologic therapies. Intra-articular injection of mesen...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：10.1007/s00005-017-0493-3
更新日期：2018-08-01 00:00:00
abstract：:The method of immunoadsorptional purification of Bean Yellow Mosaic Virus has been worked out. Immunosorbents were obtained by coupling the antibody (IgG) fraction isolated from anti-BYMV and anti-pea leaf protein antisera with CNBr-activated 1% agarose beads. Conditions for preparation of immunosorbents, for BYMV ads...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
