Abstract:
:Because a potential treatment for brain injuries could be elevating magnesium ions (Mg(2+)) intracerebrally, we characterized the effects of elevating external Mg(2+) in cultures of neonatal murine brain-derived neural stem/progenitor cells (NSCs). Using a crystal violet assay, which avoids interference of Mg(2+) in the assay, it was determined that substrate influenced Mg(2+) effects on cell numbers. On uncoated plastic, elevating Mg(2+) levels to between 2.5 and 10mM above basal increased NSC numbers, and at higher concentrations numbers decreased to control or lower levels. Similar biphasic curves were observed with different plating densities, treatment durations and length of time in culture. When cells were plated on laminin-coated plastic, NSC numbers were higher even in basal medium and no further effects were observed with Mg(2+). NSC differentiation into neurons was not altered by either substrate or Mg(2+) supplementation. Some parameters of neurite outgrowth were increased by elevated Mg(2+) when NSCs differentiated into neurons on uncoated plastic. Differentiation on laminin resulted in increased neurites even in basal medium and no further effects were seen when Mg(2+) was elevated. This system can now be used to study the multiple mechanisms by which Mg(2+) influences neuronal biology.
journal_name
Neurosci Resjournal_title
Neuroscience researchauthors
Vennemeyer JJ,Hopkins T,Kuhlmann J,Heineman WR,Pixley SKdoi
10.1016/j.neures.2014.05.001subject
Has Abstractpub_date
2014-07-01 00:00:00pages
72-8eissn
0168-0102issn
1872-8111pii
S0168-0102(14)00074-1journal_volume
84pub_type
杂志文章abstract::This study was designed to evaluate the anticonvulsant effects of imperatorin (a furanocoumarin isolated from fruits of Angelica archangelica) in the mouse maximal electroshock seizure threshold model. The threshold for electroconvulsions in mice was determined at several times: 15, 30, 60 and 120 min after i.p. admin...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2007.05.004
更新日期:2007-09-01 00:00:00
abstract::Environmental factors during perinatal period have various effects on behavior. The present study examined the effects of prenatal stress and neonatal handling on anxiety and spatial learning of offspring. Prenatal stress increased anxiety-related behavior of adult offspring, whereas neonatal handling had no effect. I...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2015.07.002
更新日期:2015-12-01 00:00:00
abstract::Spinal cord injury (SCI) is a serious condition often affecting young and healthy individuals around the world. Electro-acupuncture (EA) has been proven to contribute towards neurologic and functional recoveries in SCI, but the underlying mechanism remains largely unknown especially regarding neural specific proteins ...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2010.04.012
更新日期:2010-08-01 00:00:00
abstract::The responses of slice-cultured Purkinje cells to trans-DL-1-amino-1,3-cyclopentanedicarboxylic acid (t-ACPD) were examined by intracellular recording techniques and fura-2 microfluorometry. Bath-application of t-ACPD (100 microM, 30 s), a selective agonist of metabotropic glutamate receptors (mGluRs), to Purkinje cel...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/0168-0102(93)90125-a
更新日期:1993-03-01 00:00:00
abstract::Recent technologies and developments in neuroscience have contributed to remarkable scientific discoveries, and have also raised many new philosophical, ethical, legal, and social issues. Research in "neuroethics" has identified various ethical issues, which will be difficult for current biomedical ethics to resolve f...
journal_title:Neuroscience research
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.neures.2006.09.004
更新日期:2007-01-01 00:00:00
abstract::In the rat striatum numerous secretagogin (SCGN) positive neurons were scattered. They were heterogeneous in their morphological and chemical properties. We examined the colocalization of SCGN with known four interneuron markers, parvalbumin (PV), calretinin (CR), nitric oxide synthase (NOS) and choline acetyl transfe...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2017.01.004
更新日期:2017-06-01 00:00:00
abstract::TDP-43 pathology in motor neurons is a hallmark of ALS. In addition, the reduced expression of an RNA editing enzyme, adenosine deaminase acting on RNA 2 (ADAR2), increases the expression of GluA2 with an unedited Q/R site in the motor neurons of patients with sporadic ALS. As the occurrence of these two disease-speci...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2012.02.015
更新日期:2012-06-01 00:00:00
abstract::Acute exposure to kainic acid (KA) induces neurochemical changes in dopaminergic systems in the brain and the aim of the present study was to investigate the acute toxicity of KA upon dopamine D2 receptors. Adult rats were injected intraperitoneally with either saline or 16 mg/kg KA. Brains were removed after 4 h. Mem...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/s0168-0102(97)00130-2
更新日期:1998-03-01 00:00:00
abstract::Developmental refinement of neuronal connectivity is crucial for proper brain function. In the early phase of development, input fibers arrive at their target areas guided by specific molecular cues and form abundant immature synapses. Then, functionally important synapses are preserved and strengthened by neural acti...
journal_title:Neuroscience research
pub_type: 杂志文章,评审
doi:10.1016/j.neures.2017.06.004
更新日期:2018-04-01 00:00:00
abstract::The N-methyl-D-aspartate (NMDA) receptor 2B gene (GRIN2B) was studied as a candidate gene of alcoholism. This study aimed to investigate the association between each of the three GRIN2B polymorphisms (rs1806201, rs1805247, and rs1805502) and alcoholism. This study included 206 alcoholic patients and 189 unrelated cont...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2006.06.013
更新日期:2006-10-01 00:00:00
abstract::Previous pharmacological experiments provide conflicting findings that describe both facilitatory and inhibitory effects of neuronal histamine on learning and memory. Here, we examined learning and memory and synaptic plasticity in mice with a null mutation of gene coding histamine H1 or H2 receptor in order to clarif...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2006.10.020
更新日期:2007-02-01 00:00:00
abstract::Recent studies have compared default-mode network (DMN) connectivity in different arousal levels to investigate the relationship between consciousness and DMN. The comparison between the DMN in rapid eye movement (REM) sleep with that in non-REM (NREM) sleep is useful for revealing the relationship between arousal lev...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2010.12.018
更新日期:2011-04-01 00:00:00
abstract::To study the differences between peripheral nerve (PN) and embryonic homologous CNS structures as a conduit for CNS axons, spinal cord segments in neonatal rats were removed at the mid-thoracic level and PN or embryonic spinal cord (ESC) segments were grafted into the vacancy. Neural connections across the graft were ...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/s0168-0102(98)00042-x
更新日期:1998-07-01 00:00:00
abstract::Neural activity on cellular, regional, and behavioral levels shows context-dependence. Here we suggest the processing of input-output relationships in terms divisive normalization (DN), including (i) summing/averaging inputs and (ii) normalizing output against input stages, as a computational mechanism to underlie con...
journal_title:Neuroscience research
pub_type: 杂志文章,评审
doi:10.1016/j.neures.2019.10.002
更新日期:2020-07-01 00:00:00
abstract::We have previously shown that endothelin-B receptor stimulation increases neural progenitor proliferation, partly in G(i) and extracellular matrix molecule-dependent manner. In the present study, we investigated whether G(q/11) is also involved in this response and how G(i) and G(q/11) might regulate the extracellular...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2007.06.1478
更新日期:2007-10-01 00:00:00
abstract::It is well established that cholinergic modulation of functioning of neuronal networks is common in the central nervous system at all scales from neuronal columns to large nuclei. It is involved in various attentional, cognitive and behavioral performances. We have recently demonstrated that a frog retinotectal transm...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2012.07.002
更新日期:2012-09-01 00:00:00
abstract::Chemokines are a family of peptides originally identified as the factors regulating the migration of leukocytes in inflammatory and immune responses. Recently, they have been shown to be produced in the central and peripheral nervous systems under various pathological conditions and act on neuronal and glial cells. In...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2004.01.004
更新日期:2004-04-01 00:00:00
abstract::Swelling of brain cells is one of the physiological responses associated with neuronal activation. To investigate underlying mechanisms, we analyzed interactions between changes in cell volume and synaptic responses in the hippocampal slices from rodents. Swelling within the CA1 area was detected as increases in trans...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/s0168-0102(02)00153-0
更新日期:2002-11-01 00:00:00
abstract::Pituitary adenylate cyclase-activating polypeptide (PACAP) is a recently identified member of the secretin/vasoactive intestinal polypeptide (VIP) family. There are at least two types of receptor for PACAP: type I (PACAPR), which specifically binds PACAP; and type II (VIP/PACAPR), which binds both PACAP and VIP. The l...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/s0168-0102(97)00065-5
更新日期:1997-08-01 00:00:00
abstract::1-methyl-4-phenylpyridinium ion (MPP(+)), an inhibitor of mitochondrial complex I, has been widely used as a neurotoxin because it elicits a severe Parkinson's disease-like syndrome with elevation of intracellular reactive oxygen species (ROS) level and apoptotic death. Salvianic acid A (SA), isolated from the Chinese...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2004.10.001
更新日期:2005-02-01 00:00:00
abstract::Steroid hormones substantially influence brain development, reproduction sexual differentiation and emotion. These effects are mediated by steroid hormone receptors and cofactors, which directly regulate gene expression. Deciphering how and where these transcriptional activators occur in a cell provides the groundwork...
journal_title:Neuroscience research
pub_type: 杂志文章,评审
doi:10.1016/s0168-0102(01)00253-x
更新日期:2001-07-01 00:00:00
abstract::Maternal stress, viral infection, and obstetric complications, which trigger cytokine signaling, are hypothesized to be involved in schizophrenia and its related disorders. The etiologic contribution of individual cytokines to such psychiatric disorders, however, remains to be evaluated. To estimate the impact of peri...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2004.05.010
更新日期:2004-09-01 00:00:00
abstract::Interleukin-6 (IL-6, human recombinant) promoted the survival of catecholaminergic neurons from fetal and postnatal rat midbrains as assessed by an immunohistochemical staining for tyrosine hydroxylase (TH) in culture using a chemically defined medium. The maximal dose of IL-6 for the cell survival of postnatal P15 ra...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/0168-0102(92)90039-f
更新日期:1992-05-01 00:00:00
abstract::Development of neurofibrillary tangles (NFTs) is a pathological hallmark in various neurodegenerative disorders including Alzheimer's disease (AD). Recently, we identified a granular tau oligomer having a pre-filamentous structure. To determine the role of this oligomer in NFT formation, we quantified the amount of gr...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2005.11.009
更新日期:2006-03-01 00:00:00
abstract::This work was aimed to assess whether voluntary exercise rescued behavioral and hippocampal alterations in mice lacking the lysophosphatidic acid LPA1 receptor (LPA1-null mice), studying the potential relationship between the amount of exercise performed and its effects. Normal and LPA1-null mice underwent 23 days of ...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2013.09.004
更新日期:2013-11-01 00:00:00
abstract::This experiment tested the effect of haloperidol on the sympathetic and thermogenic effects induced by orexin A. The firing rates of the sympathetic nerves to interscapular brown adipose tissue (IBAT), along with IBAT and colonic temperatures and heart rate were monitored in urethane-anesthetized male Sprague-Dawley r...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/s0168-0102(02)00191-8
更新日期:2003-01-01 00:00:00
abstract::Tissue-type plasminogen activator (tPA) is a serine protease that converts the inactive precursor plasminogen to the active protease plasmin. In the central nervous system, tPA has been suggested to participate in plasticity, memory and the neuronal degeneration caused by excitotoxins, but its precise functions during...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/s0168-0102(98)00125-4
更新日期:1999-02-01 00:00:00
abstract::Transforming growth factor beta (TGF-beta) is an extracellular signaling molecule known to mediate programmed cell death (PCD) in the developing retina. In the present study, we investigated the expression profiles and activity levels of TGF-beta ligand and TGF-beta receptors (TbetaR) during the successive physiologic...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2006.07.002
更新日期:2006-10-01 00:00:00
abstract::Forebrain heat shock protein 70 (HSP70) immunohistochemical reactivity was investigated in rats subjected to gamma knife irradiation focusing on the right caudate putamen nucleus. The forebrain sections of all experimental animals were processed with anti-HSP70 antiserum and then by avidin-biotin peroxidase complex im...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/s0168-0102(00)00134-6
更新日期:2000-10-01 00:00:00
abstract::Short-interval intracortical inhibition (SICI) acting on the first dorsal interosseus was measured using paired-pulse transcranial magnetic stimulation (interstimulus interval=2ms) in samples of young and healthy older subjects and correlated with manual dexterity measured with the Purdue Pegboard test and two isometr...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2011.04.004
更新日期:2011-08-01 00:00:00