Abstract：
:Homogenates of rat ventral and dorsal spinal cords were subjected to differential and gradient centrifugation on linear sucrose gradients following lysis of a synaptosomal fraction. The distribution of calcitonin gene-related peptide (CGRP) and substance P (SP) in dorsal spinal cord as well as the distribution of serotonin (5-HT) and SP in ventral spinal cord was determined, using radioimmunoassay (RIA) for CGRP and SP and high-performance liquid chromatography for 5-HT. In dorsal spinal cord. CGRP and SP had an almost identical distribution, with one peak in an intermediate density fraction which according to electron microscopy contained i.a. large dense-cored vesicles, and a second peak in a heavy fraction enriched in synaptosome-like structures. In ventral spinal cord, 5-HT and SP had different distribution patterns; 5-HT peaked in a light fraction containing many small synaptic vesicles, whereas SP peaked in an intermediate density fraction similar to the one in dorsal spinal cord. Both 5-HT and SP had second peaks in high density fractions containing synaptosome-like structures. The study demonstrates that the peptides CGRP and SP, partly coexisting in dorsal spinal cord, both seem to be stored in large vesicles inside synaptosomes, whereas the amine 5-HT and the peptide SP, coexisting in ventral spinal cord, appear to have a differential storage with 5-HT mainly in small vesicles and SP in large vesicles, both inside synaptosomes.
journal_name
Brain Resjournal_title
Brain researchauthors
Fried G,Franck J,Brodin E,Born W,Fischer JA,Hiort W,Hökfelt Tdoi
10.1016/0006-8993(89)90780-4subject
Has Abstractpub_date
1989-10-16 00:00:00pages
315-24issue
2eissn
0006-8993issn
1872-6240pii
0006-8993(89)90780-4journal_volume
499pub_type
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