Abstract：
:A gene encoding halohydrin dehalogenase (HHDH) from Agrobacterium tumefaciens CCTCC M 87071 was cloned and expressed in Escherichia coli. To increase activity and stability of HHDH, 14 amino acid residues around the active site and substrate-binding pocket based on the structural analysis and molecular docking were selected as targets for site-directed mutagenesis. The studies showed that the mutant HHDH (Mut-HHDH) enzyme had a more accessible substrate-binding pocket than the wild-type HHDH (Wt-HHDH). Molecular docking revealed that the distance between the substrate and active site was closer in mutant which improved the catalytic activity. The expressed Wt-HHDH and Mut-HHDH were purified and characterized using 1,3-dichloro-2-propanol (1,3-DCP) as substrates. The specific activity of the mutant was enhanced 26-fold and the value of k cat was 18.4-fold as compared to the Wt-HHDH, respectively. The Mut-HHDH showed threefold extension of half-life at 45 °C than that of Wt-HHDH. Therefore it is possible to add 1,3-DCP concentration up to 100 mM and epichlorohydrin (ECH) was produced at a relatively high conversion and yield (59.6 %) using Mut-HHDH as catalyst. This Mut-HHDH could be a potential candidate for the upscale production of ECH.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Liu ZQ,Gao AC,Wang YJ,Zheng YG,Shen YCdoi
10.1007/s10295-014-1443-2subject
Has Abstractpub_date
2014-07-01 00:00:00pages
1145-58issue
7eissn
1367-5435issn
1476-5535journal_volume
41pub_type
杂志文章abstract：:Huperzia serrata is a producer of huperzine A (HupA), a cholinesterase inhibitor (ChEI). Over 120 endophytic fungi were recovered from this plant and screened for Hup-A and nine were found. These nine represented seven different fungal genera with the most significant producer being Shiraia sp. A total of 127 endophyt...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-010-0905-4
更新日期：2011-09-01 00:00:00
abstract：:Natural product discovery in the microbial world has historically been biased toward aerobes. Recent in silico analysis demonstrates that genomes of anaerobes encode unexpected biosynthetic potential for natural products, however, chemical data on natural products from the anaerobic world are extremely limited. Here, ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s10295-018-2086-5
更新日期：2019-03-01 00:00:00
abstract：:Lactobacillus casei Zhang is a widely recognized probiotic bacterium, which is being commercially used in China. To study the gene expression dynamics of L. casei Zhang during fermentation in soymilk, a whole genome microarray was used to screen for differentially expressed genes when grown to the lag phase, the late ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-011-1015-7
更新日期：2012-01-01 00:00:00
abstract：:This paper summarizes research results and their industrial applications obtained by continuous culture in the former Czechoslovakia. Past achievements as well as recent trends and developments worldwide are presented. The term "Prague School of continuous culture" is put forward and its international activity is outl...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 历史文章,杂志文章
doi：10.1007/s10295-010-0881-8
更新日期：2010-12-01 00:00:00
abstract：:A novel lipolytic enzyme was isolated from a metagenomic library after demonstration of lipolytic activity on an LB agar plate containing 1% (w/v) tributyrin. A novel esterase gene (estIM1), encoding a lipolytic enzyme (EstIM1), was cloned using a shotgun method from a pFosEstIM1 clone of the metagenomic library, and ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-011-1080-y
更新日期：2012-05-01 00:00:00
abstract：:Providencia rettgeri strain YL was found to be efficient in heterotrophic nitrogen removal under aerobic conditions. Maximum removal of NH(4) (+)-N occurred under the conditions of pH 7 and supplemented with glucose as the carbon source. Inorganic ions such as Mg(2+), Mn(2+), and Zn(2+) largely influenced the growth a...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-010-0708-7
更新日期：2010-06-01 00:00:00
abstract：:A commercial process was developed for producing a recombinant vaccine against hydatidosis in farm animals. The vaccine antigen consisting of a surface protein of the oncospheres of the hydatid worm (Echinococcus granulosus), was produced as inclusion bodies in Escherichia coli. Fed-batch cultures of E. coli using Ter...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-005-0046-3
更新日期：2006-03-01 00:00:00
abstract：:Deficiency in petroleum resources and increasing environmental concerns have pushed a bio-based economy to be built, employing a highly reproducible, metal contaminant free, sustainable and green biomanufacturing method. Here, a chiral drug intermediate L-pipecolic acid has been synthesized from biomass-derived lysine...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-018-2044-2
更新日期：2018-06-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-019-02245-8
更新日期：2020-01-01 00:00:00
abstract：:Rapid fluorescence techniques were evaluated for the detection of bacterial contaminants in papermaking chemicals including starch and the resin-based sizes and starch slurries used in the paper industry. Viable and non-viable bacterial cells were visualised by fluorescent probes and detected by epifluorescence micros...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-003-0046-0
更新日期：2003-04-01 00:00:00
abstract：:Biological synthesis of plant secondary metabolites has attracted increasing attention due to their proven or assumed beneficial properties and health-promoting effects. Phenylpropanoids are the precursors to a range of important plant metabolites such as the secondary metabolites belonging to the flavonoid/stilbenoid...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-011-0954-3
更新日期：2011-10-01 00:00:00
abstract：:The thermoacidophilic archaeon Sulfolobus solfataricus MT4 encodes a maltooligosyltrehalose synthase (MTS), that catalyzes an intramolecular transglycosylation process converting the glycosidic linkages at the reducing end of dextrins from alpha-1,4 into alpha-1,1. In this research the gene encoding MTS was cloned and...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-008-0384-z
更新日期：2008-10-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-006-0202-4
更新日期：2007-04-01 00:00:00
abstract：:Lasiodiplodan, an exopolysaccharide of the (1→6)-β-D: -glucan type, is produced by Lasiodiplodia theobromae MMPI when grown under submerged culture on glucose. The objective of this study was to evaluate lasiodiplodan production by examining the effects of carbon (glucose, fructose, maltose, sucrose) and nitrogen sour...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-012-1112-2
更新日期：2012-08-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-008-0475-x
更新日期：2009-01-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj.jim.7000097
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj.jim.2900446
更新日期：1997-08-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj.jim.2900368
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2001-07-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2001-11-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2003-03-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2007-08-01 00:00:00
abstract：:Bacterial biofilms of Pseudomonas aeruginosa selectively labeled by introduction of 2-13C-glycerol was studied by solid-state and high-resolution nuclear magnetic resonance. The 13C nuclei were mainly integrated into mannuronate and guluronate, the two monomer units forming the bacterial alginate. The signal for the C...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
