Abstract：
:Accurate optical reporting of electrical activity in genetically defined neuronal populations is a long-standing goal in neuroscience. We developed Accelerated Sensor of Action Potentials 1 (ASAP1), a voltage sensor design in which a circularly permuted green fluorescent protein is inserted in an extracellular loop of a voltage-sensing domain, rendering fluorescence responsive to membrane potential. ASAP1 demonstrated on and off kinetics of ∼ 2 ms, reliably detected single action potentials and subthreshold potential changes, and tracked trains of action potential waveforms up to 200 Hz in single trials. With a favorable combination of brightness, dynamic range and speed, ASAP1 enables continuous monitoring of membrane potential in neurons at kilohertz frame rates using standard epifluorescence microscopy.
journal_name
Nat Neuroscijournal_title
Nature neuroscienceauthors
St-Pierre F,Marshall JD,Yang Y,Gong Y,Schnitzer MJ,Lin MZdoi
10.1038/nn.3709subject
Has Abstractpub_date
2014-06-01 00:00:00pages
884-9issue
6eissn
1097-6256issn
1546-1726pii
nn.3709journal_volume
17pub_type
杂志文章abstract：:A cognitive map has long been the dominant metaphor for hippocampal function, embracing the idea that place cells encode a geometric representation of space. However, evidence for predictive coding, reward sensitivity and policy dependence in place cells suggests that the representation is not purely spatial. We appro...
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abstract：:Microcircuits composed of principal neuron and interneuron dendrites have an important role in shaping the representation of sensory information in the olfactory bulb. Here we establish the physiological features governing synaptic signaling in dendrodendritic microcircuits of olfactory bulb glomeruli. We show that de...
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更新日期：2015-05-01 00:00:00
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