Abstract:
:The relationships between bilayer lipid, diffusional and conformational activities of oxidoreduction components, and electron transfer activity in the mitochondrial inner membrane are considered. Using a new, low pH method to fuse liposome phospholipid (asolectin) with the isolated mitochondrial inner membrane, the membrane bilayer is enriched up to 700% with exogenous phospholipid. During such enrichment, ultrastructural analysis reveals that integral proteins diffuse freely and randomly into the expanding bilayer. Kinetic analysis reveals that a diffusion limited step occurs between succinate- and NADH dehydrogenase and cytochromes bc1, and that the dehydrogenases, ubiquinone, and cytochromes bc1 are free to diffuse independently of one another in the membrane plane. Whether cytochromes bc1 and cytochrome c oxidase codiffuse in the membrane plane, or diffuse independently of one another remains unclear. The specific activities of succinate- and NADH-dehydrogenase as well as cytochrome c oxidase are affected by bilayer enrichment. This most likely occurs through the direct modulation by the newly incorporated phospholipid on conformational activity required in the oxidoreductases for electron transfer.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Hackenbrock CR,Schneider H,Lemasters JJ,Höchli Mdoi
10.1007/978-1-4757-1419-7_26subject
Has Abstractpub_date
1980-01-01 00:00:00pages
245-63eissn
0065-2598issn
2214-8019journal_volume
132pub_type
杂志文章abstract::The influence of Ca2+ dissociation rate from TnC and decreased cross-bridge detachment rate on the time course of relaxation induced by flash photolysis of diazo-2 in rabbit skinned psoas fibers was investigated at 15 degrees C. A TnC mutant (M82Q TnC) that exhibited increased Ca2+ sensitivity caused by a decreased Ca...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4419-9029-7_51
更新日期:2003-01-01 00:00:00
abstract::Cultured human fibroblasts accumulated methotrexate polyglutamates to levels far in excess of the dihydrofolate reductase binding capacity. After four days in methotrexate-free medium the intracellular drug level dropped by 70% but nearly 80% of the remaining methotrexate was in the form of polyglutamates. Reduced fol...
journal_title:Advances in experimental medicine and biology
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doi:10.1007/978-1-4757-5241-0_21
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-2946-0_21
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-4709-9_79
更新日期:1999-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
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更新日期:2010-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-1371-1_12
更新日期:2001-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-1875-4_113
更新日期:1994-01-01 00:00:00
abstract::Taurine plays a protective role against free radicals and toxins in various cells and tissues. However, the effect of taurine on hepatic injury and fibrosis developed by activated hepatic stellate cells (HSC) and myofibroblast-like cells is not fully understood. We investigated the effects of taurine on the hepatic fi...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-0-387-75681-3_30
更新日期:2009-01-01 00:00:00
abstract::Cardiovascular disease remains the leading cause of morbidity and mortality for both women and men. Emerging evidence supports that ischemic heart disease (IHD) may manifest differently in women and men, in ways ranging from the clinical presentation, diagnosis, and management of disease to the basic biology and biome...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-319-77932-4_17
更新日期:2018-01-01 00:00:00
abstract::TRPCs have been demonstrated to be widely expressed in different cancers. In recent years, a number of studies closely investigated the roles of TRPCs in cancer cells. Most of the results show that both mRNA and protein levels of TRPCs significantly increase in cancer tissues compared with healthy controls. TRPCs regu...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
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更新日期:2017-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-319-55231-6_1
更新日期:2017-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4419-1050-9_1
更新日期:2010-01-01 00:00:00
abstract::This contribution on the biology and management of bone metastases from prostatic cancer is divided into three parts. The first details a study conducted at Stanford University on the prevention of bone metastases in the lumbar spine, in patients in whom the lumbar spine has been irradiated coincidental to the radiati...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4615-3398-6_28
更新日期:1992-01-01 00:00:00
abstract::Methods to develop an immuno-optical biosensor for the detection and monitoring of Protein C (PC) concentrations are described. A tapered quartz fiber is enclosed in a glass tube (capacity approximately 300 microliters) and monoclonal antibody against PC (anti-PC) is immobilized on the surface of this fiber. PC within...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-4863-8_81
更新日期:1998-01-01 00:00:00
abstract::Pulmonary hypertension (PH) is a deadly and increasingly prevalent vascular disease characterized by excessive pulmonary vascular remodeling and right ventricular dysfunction which leads to right heart failure, multiorgan dysfunction, and premature death. The cause of the vascular remodeling in PH remains elusive, and...
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pub_type: 杂志文章,评审
doi:10.1007/978-3-319-63245-2_24
更新日期:2017-01-01 00:00:00
abstract::Genetic transformation is a critical component to the fundamental genetic analysis of insect species and holds great promise for establishing strains that improve population control and behavior for practical application. This is especially so for insects that are disease vectors, many of which are currently subject t...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
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更新日期:2008-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
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更新日期:2018-01-01 00:00:00
abstract::In this communication I have attempted to present an overview of some contributions to the understanding of the oviduct-egg interaction in amphibians. According to data from other authors, the vitelline envelope of the newly ovulated egg constitutes a barrier for the passage of spermatozoa. Our results demonstrated th...
journal_title:Advances in experimental medicine and biology
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更新日期:1986-01-01 00:00:00
abstract::There are two alternative approaches to ultrasound-assisted drug delivery. First, the drug can be entrapped into or attached onto the ultrasound-responsive particles and administered in the vasculature, to achieve ultrasound-triggered drug release from the particles and localized tissue deposition in response to ultra...
journal_title:Advances in experimental medicine and biology
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更新日期:2016-01-01 00:00:00
abstract::Wound healing is a complex process with the considerable burden on healthcare system. There are several cellular therapy methods that have been introduced to treat different types of wounds. Despite the advantages of cellular therapy, it is needed to overcome different limitations of this method such as; tumorigenicit...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
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更新日期:2018-01-01 00:00:00
abstract::Nitric oxide (NO) regulates normal vasomotor tone and modulates important homeostatic functions such as thrombosis, cellular proliferation, and adhesion molecule expression. Recent data implicate a critical function for hemoglobin and the erythrocyte in regulating the bioavailability of NO in the vascular compartment....
journal_title:Advances in experimental medicine and biology
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更新日期:2006-01-01 00:00:00
abstract::The kisspeptin system is considered to be essential for successful mammalian reproduction. In addition to the Kiss1 peptide, Kiss2, the product of kiss2 (the kiss1 paralogue), has also been shown to activate kisspeptin receptor signaling pathways in nonmammalian species. Furthermore, in nonmammalian species, there are...
journal_title:Advances in experimental medicine and biology
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更新日期:2013-01-01 00:00:00
abstract::Techniques of rare event dynamics were reviewed including the string methods, which will be implemented with the biochemical simulation packages. The existing methods were applied to study biological systems with relevance to drug design and drug metabolism. The rare event dynamics simulations were performed to unders...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-94-017-9245-5_6
更新日期:2015-01-01 00:00:00
abstract::Schizophrenia alters basic brain processes of perception, emotion, and judgment to cause hallucinations, delusions, thought disorder, and cognitive deficits. Unlike neurodegeneration diseases that have irreversible neuronal degeneration and death, schizophrenia lacks agreeable pathological hallmarks, which makes it on...
journal_title:Advances in experimental medicine and biology
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更新日期:2012-01-01 00:00:00
abstract::This chapter represents a novel view of modeling in hematopoiesis, synthesizing both deterministic and stochastic approaches. Whereas the stochastic models work in situations where chance dominates, for example when the number of cells is small, or under random mutations, the deterministic models are more important fo...
journal_title:Advances in experimental medicine and biology
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更新日期:2014-01-01 00:00:00
abstract::It is well known that patients who undergo surgical operations have a high risk of infection and sepsis. One explanation for this high risk may be a depression of neutrophil functions at the postoperative period. In the present study, the effects of surgical stresses on neutrophil functions were studied in ten patient...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-9543-4_34
更新日期:1989-01-01 00:00:00
abstract::Type 1 diabetes mellitus (T1DM) is classified as an autoimmune disease which progressively results in the depletion of insulin-secreting β-cells. Consequently, the insulin secretion stops leading to hyperglycemic situations within the body. Under severe conditions, it also causes multi-organ diabetes-associated dysfun...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/5584_2018_195
更新日期:2019-01-01 00:00:00