Abstract:
OBJECTIVE:Our study aim was to determine encapsulated islet graft viability in an omentum pouch and the effect of fibroblast growth factor 1 (FGF-1) released from our redesigned alginate microcapsules on the function of the graft. METHODS:Isolated rat islets were encapsulated in an inner core made with 1.5% low-viscosity-high-mannuronic-acid alginate followed by an external layer made with 1.25% low-viscosity high-guluronic acid alginate with or without FGF-1, in microcapsules measuring 300 to 400 µm in diameter. The 2 alginate layers were separated by a perm-selective membrane made with 0.1% poly-L-ornithine, and the inner low-viscosity-high-mannuronic-acid core was partially chelated using 55 mM sodium citrate for 2 minutes. RESULTS:A marginal mass of encapsulated islet allografts (∼2000 islets/kg) in streptozotocin-diabetic Lewis rats caused significant reduction in blood glucose levels similar to the effect observed with encapsulated islet isografts. Transplantation of alloislets coencapsulated with FGF-1 did not result in better glycemic control, but induced greater body weight maintenance in transplant recipients compared with those that received only alloislets. Histological examination of the retrieved tissue demonstrated morphologically and functionally intact islets in the microcapsules, with no signs of fibrosis. CONCLUSIONS:We conclude that the omentum is a viable site for encapsulated islet transplantation.
journal_name
Pancreasjournal_title
Pancreasauthors
Pareta R,McQuilling JP,Sittadjody S,Jenkins R,Bowden S,Orlando G,Farney AC,Brey EM,Opara ECdoi
10.1097/MPA.0000000000000107subject
Has Abstractpub_date
2014-05-01 00:00:00pages
605-13issue
4eissn
0885-3177issn
1536-4828journal_volume
43pub_type
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