Purification and characterization of pepsin-solubilized collagen from skin of sea cucumber Holothuria parva.

abstract：:Vacuolar processing enzymes (VPEs) have received considerable attention recently, as they exhibit caspase-1-like cleavage activity and regulate the process of PCD. However, knowledge about their detailed characteristics and structures is relatively limited. In this study, a gamma vacuolar processing enzyme gene, MhVPE...

journal_title：Applied biochemistry and biotechnology

authors： Ran K,Yang H,Sun X,Li Q,Jiang Q,Zhang W,Shen W

更新日期：2014-05-01 00:00:00

abstract：:A comparative analysis of the structural and functional aspects along with equilibrium unfolding of two homologous cutinases, Cut1 and Cut2, from Thermobifida fusca was carried out. The CD and fluorescence profile at different pH in the range of 6 to 9 showed no structural variations for both cutinases, indicating the...

journal_title：Applied biochemistry and biotechnology

authors： Hegde K,Dasu VV

更新日期：2014-09-01 00:00:00

abstract：:The role of selenium (Se) supplementation on glutathione, a potent intracellular redox buffer, and trehalose, a well-known stress protectant molecule, was studied. The amount of glutathione decreased significantly while that of trehalose showed a minor decrease in the cells grown in Se-supplemented medium. After heat ...

journal_title：Applied biochemistry and biotechnology

authors： Sharma SC,Anand MS

更新日期：2006-04-01 00:00:00

abstract：:The present study deals with the characterization of halotolerant protease produced by Bacillus aquimaris VITP4 strain isolated from Kumta coast, Karnataka, India. The studies were performed at 40 °C and pH 8 in Tris buffer. Metal ions such as Mn(2+) and Ca(2+) increased the proteolytic activity of the enzyme by 34 an...

journal_title：Applied biochemistry and biotechnology

authors： Thaz CJ,Jayaraman G

更新日期：2014-01-01 00:00:00

abstract：:Hydrogels are attractive biomaterials for three-dimensional cell culture and tissue engineering applications. The preparation of hydrogels using alginate and gelatin provides cross-linked hydrophilic polymers that can swell but do not dissolve in water. In this work, we first reinforced pure alginate by using polyoxye...

journal_title：Applied biochemistry and biotechnology

authors： Aroguz AZ,Baysal K,Adiguzel Z,Baysal BM

更新日期：2014-05-01 00:00:00

abstract：:The two six-coordinate Pt(IV) complexes, containing bidentate nitrogen donor/methyl ligands with general formula [Pt(X)2Me2((t)bu2bpy)], where (t)bu2bpy = 4,4'-ditert-butyl-2,2'-bipyridine and X = Cl (C1) or Br (C2), serving as the leaving groups were synthesized for evaluation of their anticancer activities and DNA b...

journal_title：Applied biochemistry and biotechnology

authors： Pouryasin Z,Yousefi R,Nabavizadeh SM,Rashidi M,Hamidizadeh P,Alavianmehr MM,Moosavi-Movahedi AA

更新日期：2014-03-01 00:00:00

abstract：:Biological control of soil-borne plant pathogens is a potential alternative to the use of chemical pesticides, which have already been proved to be harmful to the environment. Several strains of the fungus Trichoderma have been isolated and found to be effective biocontrol agents of various soil-borne plant pathogenic...

journal_title：Applied biochemistry and biotechnology

authors： Chet I,Inbar J

更新日期：1994-07-01 00:00:00

abstract：:Sugarcane bagasse is the major by-product of the sugar industry. It has a great potential for the production of biofuels and chemicals due to its considerable amount of cellulose and hemicellulose. In this study, we investigated a simple and economic pretreatment process using dilute ammonia for the storage of sugarca...

journal_title：Applied biochemistry and biotechnology

authors： Kim M,Aita G,Day DF

更新日期：2010-05-01 00:00:00

abstract：:The metabolism of residual glycerol from biodiesel synthesis by Klebsiella pneumoniae BLh-1 was investigated in this study. Batch and fed-batch cultivations were performed in bioreactors under anaerobic and oxygen limitation conditions. Results of batch cultivations showed that the main product was 1,3-propanediol (1,...

journal_title：Applied biochemistry and biotechnology

authors： Rossi DM,de Souza EA,Ayub MA

更新日期：2013-03-01 00:00:00

abstract：:Observation of the ultrastructural and intracellular chemical changes of CaCl2-tolerant strains is important both for understanding their adaptation mechanism under high salt stress and for providing theoretical basis of their application in treating high-CaCl2 wastewaters. A novel strain V 430 isolated by us has been...

journal_title：Applied biochemistry and biotechnology

authors： Xin X,Wang Y

更新日期：2007-09-01 00:00:00

abstract：:An anaerobic sequencing batch reactor containing immobilized biomass (AnSBBR) was used to produce biomethane by treating the effluent from another AnSBBR used to produce biohydrogen from glucose- (AR-EPHG) and sucrose-based (AR-EPHS) wastewater. In addition, biomethane was also produced from sucrose-based synthetic wa...

journal_title：Applied biochemistry and biotechnology

authors： Lullio TG,Souza LP,Ratusznei SM,Rodrigues JA,Zaiat M

更新日期：2014-11-01 00:00:00

abstract：:An extracellular polygalacturonase (PG) produced from Paecilomyces variotii was purified to homogeneity through two chromatography steps using DEAE-Fractogel and Sephadex G-100. The molecular weight of P. variotii PG was 77,300 Da by gel filtration and SDS-PAGE. PG had isoelectric point of 4.37 and optimum pH 4.0. PG ...

journal_title：Applied biochemistry and biotechnology

authors： de Lima Damásio AR,da Silva TM,Maller A,Jorge JA,Terenzi HF,Polizeli Mde L

更新日期：2010-03-01 00:00:00

abstract：:A soluble glucoside 3-dehydrogenase (G3DH) was purified from a newly isolated Sphingobacterium faecium ZJF-D6 CCTCC M 2013251. The enzyme was purified to 35.71-fold with a yield of 41.91 % and was estimated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis with a molecular mass of 62 kDa. The sequences of ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang JF,Yang B,Chen W,Chen J

更新日期：2014-04-01 00:00:00

abstract：:Proteases, lipases, amylases, and cellulases are enzymes used in detergent formulation to improve the detergency. The amylases are specifically supplemented to the detergent to digest starchy stains. Most of the solid and liquid detergents that are currently manufactured contain alkaline enzymes. The advantages of usi...

journal_title：Applied biochemistry and biotechnology

authors： Niyonzima FN,More SS

更新日期：2014-10-01 00:00:00

abstract：:The effect of organic matter and fill time on anaerobic sequencing batch reactor (5 L, 30 °C, 8-h cycles, 50 rpm) efficiency has been analyzed. Organic matter was increased by the influent concentration. Fill times investigated were in the batch mode and fed-batch followed by batch. In the batch mode organic matter re...

journal_title：Applied biochemistry and biotechnology

authors： Selma VC,Cotrim LH,Rodrigues JA,Ratusznei SM,Zaiat M,Foresti E

更新日期：2010-12-01 00:00:00

abstract：:L-Asparaginase (ASNase) has proved its use in medical and food industries. Sequence-based screening showed the thermophilic Streptomyces strain Streptomyces thermoluteus subsp. fuscus NBRC 14270 (14270 ASNase) to positive against predicted ASNase primary sequences. The 14270 ASNase gene and four L-asparaginase genes f...

journal_title：Applied biochemistry and biotechnology

authors： Hatanaka T,Usuki H,Arima J,Uesugi Y,Yamamoto Y,Kumagai Y,Yamasato A,Mukaihara T

更新日期：2011-04-01 00:00:00

abstract：:Chemical composition of Larrea tridentata leaves was determined and elevated content of lignin (35.96 % w/w) was found. The present study was proposed in order to evaluate the extraction of bioactive compounds, particularly phenolic compounds, by solid-state fermentation (SSF) of L. tridentata leaves. The basidiomycet...

journal_title：Applied biochemistry and biotechnology

authors： Martins S,Teixeira JA,Mussatto SI

更新日期：2013-11-01 00:00:00

abstract：:Alpha-lactalbumin hydrolysate is of significant interest, due to its potential application as a source of bioactive peptides in nutraceutical and pharmaceutical domains. This study was focused on the cholecystokinin (CCK) family compounds which are small peptides involved in the satiety control. The action of chymotry...

journal_title：Applied biochemistry and biotechnology

authors： Catiau L,Delval-Dubois V,Guillochon D,Nedjar-Arroume N

更新日期：2011-11-01 00:00:00

abstract：:In biotechnological processes, a great number of factors can influence the income productivity and conversion. Normally, it is not evident which of these factors are the most important and how they interact. In this work, multivariate analysis techniques are used as experimental design coupled to a detailed determinis...

journal_title：Applied biochemistry and biotechnology

authors： Lunelli BH,Rivera EC,Vasco de Toledo EC,Wolf Maciel MR,Maciel Filho R

更新日期：2008-03-01 00:00:00

abstract：:This study proposes an innovative strategy of lignocellulose biodegradation by Inonotus obliquus under solid-state fermentation in extracting Eucommia ulmoides trans-1,4-polyisoprene (EUG) and producing reducing sugars efficiently. EUG and sugars were obtained through the white rot fungal pretreatment of E. ulmoides l...

journal_title：Applied biochemistry and biotechnology

authors： Qian S,Zhang C,Zhu Z,Huang P,Xu X

更新日期：2020-11-01 00:00:00

abstract：:Trehalose synthase (TreS) from Meiothermus ruber was co-aggregated with polyethyleneimine (PEI) and precipitated with polyethylene glycol (PEG), followed by cross-linking with glutaraldehyde to obtain TreS-polyethyleneimine cross-linked enzyme aggregates (termed as CLEAs-PEI-PEG). The TreS solution at 0.5 mg mL(-1) pr...

journal_title：Applied biochemistry and biotechnology

authors： Zheng J,Chen Y,Yang L,Li M,Zhang J

更新日期：2014-11-01 00:00:00

abstract：:Further characterization of an aspartyl protease from Mucor bacilliformis with milk-clotting activity was performed. An extinction coefficient, epsilon 278 cm = 1.61 mL/mg/cm, a molecular mass of 35,400 Da and a pI of 5.2 were determined. Proteolytic activity and kinetic parameters were evaluated by using the hexapept...

journal_title：Applied biochemistry and biotechnology

authors： Venera GD,Machalinski C,Zumárraga H,Biscoglio de Jiménez Bonino MJ

更新日期：1997-12-01 00:00:00

abstract：:Cofermentation of glucose, xylose, and arabinose is critical for complete bioconversion of lignocellulosic biomass, such as agricultural residues and herbaceous energy crops, to ethanol. We have previously developed a plasmid-bearing strain of Zymomonas mobilis (206C[pZB301]) capable of cofermenting glucose, xylose, a...

journal_title：Applied biochemistry and biotechnology

authors： Mohagheghi A,Evans K,Chou YC,Zhang M

更新日期：2002-04-01 00:00:00

abstract：:Degradation of known odor precursors in sludge during anaerobic digestion was systematically studied and simulated using the Anaerobic Digestion Model Number 1 (ADM1). The degradation of various protein fractions (particulate, soluble, and bound), volatile fatty acids (VFAs), lipids, and amino acids of primary sludge ...

journal_title：Applied biochemistry and biotechnology

authors： Aldin S,Tu F,Nakhla G,Ray MB

更新日期：2011-08-01 00:00:00

abstract：:Oil palm empty fruit bunch (EFB) is abundantly available in Malaysia and it is a potential source of xylose for the production of high-value added products. This study aimed to optimize the hydrolysis of EFB using dilute sulfuric acid (H2SO4) and phosphoric acid (H3PO4) via response surface methodology for maximum xyl...

journal_title：Applied biochemistry and biotechnology

authors： Tan HT,Dykes GA,Wu TY,Siow LF

更新日期：2013-08-01 00:00:00

abstract：:The ability of poly(ethylene glycol) (PEG) to protect enzymatic peroxidase activity was determined for horseradish peroxidase (HRP), versatile peroxidase (VP), commercial Coprinus peroxidase (BP), and chloroperoxidase (CPO). The operational stability measured as the total turnover number was determined for the four pe...

journal_title：Applied biochemistry and biotechnology

authors： Juarez-Moreno K,Ayala M,Vazquez-Duhalt R

更新日期：2015-11-01 00:00:00

abstract：:Phycocyanin is a photosynthetic pigment found in photosynthetic cyanobacteria, cryptophytes, and red algae. In general, production of phycocyanin depends mainly on the light conditions during the cultivation period, and purification of phycocyanin requires expensive and complex procedures. In this study, we propose a ...

journal_title：Applied biochemistry and biotechnology

authors： Lee SH,Lee JE,Kim Y,Lee SY

更新日期：2016-01-01 00:00:00

abstract：:The factors affecting decolorization of anthraquinone dye represented by Reactive Blue 4 (RB4) and azo dye represented by Methyl Orange (MO) were studied in batch experiments under mesophilic (35 degrees C) and thermophilic (55 degrees C) anaerobic conditions. The results indicated differences in decolorization proper...

journal_title：Applied biochemistry and biotechnology

authors： Boonyakamol A,Imai T,Chairattanamanokorn P,Higuchi T,Sekine M

更新日期：2009-07-01 00:00:00

abstract：:The 1,3-dihydroxyacetone (DHA)-overproducing mutant of Gluconobacter oxydans was screened via UV mutagenesis to enhance the DHA production, and the DHA fermentation condition was optimized using the dissolved oxygen (DO) control strategy. The stable mutant G. oxydans ZJB11001 exhibits high DHA productivity and can tol...

journal_title：Applied biochemistry and biotechnology

authors： Hu ZC,Zheng YG

更新日期：2011-11-01 00:00:00

abstract：:Currently, fumaric acid is produced by catalytic isomerization of maleic acid in aqueous solutions at low pH. Being petroleum based, requiring catalyst, and producing vast amounts of by-products and wastewater, the production of fumaric acid from renewable resources by a "green" process is increasingly attractive. In ...

journal_title：Applied biochemistry and biotechnology

authors： Liu Y,Song J,Tan T,Liu L

更新日期：2015-03-01 00:00:00