Comparative study of autophagy inhibition by 3MA and CQ on Cytarabine‑induced death of leukaemia cells.

Abstract:

BACKGROUND:As the molecular mechanisms of Cytarabine,one of the most important drugs used in the leukaemia’s treatment, are only partially understood and the role of autophagy on leukaemia development and treatment is only recently being investigated, in this study, by using Chloroquine (CQ) and 3-methyladenine (3MA) as autophagy inhibitors, we aim to evaluate the contribution of an autophagic mechanism to Cytarabine (AraC)-induced death of HL60 leukaemia cells. METHODS:Trypan blue exclusion and AnnexinV/PI assays were used to evaluate HL60 cell death under AraC treatment in the presence or absence of 3MA and CQ. Western blotting and immunofluorescence experiments were performed to show the involvement of apoptosis and autophagy protein expressions. Phenotypic characterization of HL60-treated cells was performed by using immunophenotyping. Clonogenic assays were applied to analyse clonal function of HL60-treated cells. RESULTS:We observed that although autophagy inhibition by 3MA, but not CQ, increased the death of HL60 AraC cells after 24 h of treatment, no significant differences between AraC and AraC + 3MA-treated groups were observed by using clonogenic assay. In addition, increased number of immature (CD34(+)/CD38(−)Lin(−/low)) HL60 cells was found in AraC and AraC-3MA groups when compared with control untreated cells. CONCLUSIONS:Although AraC anti-leukaemia effects could be potentiated by 3MA autophagy inhibition after 24 h of exposure, leukaemia cell resistance, the main causes of treatment failure, is also promoted by autophagy initial stage impairment by 3MA, denoting the complex role of autophagy in leukaemia cells’ response to chemotherapy.

authors

Palmeira dos Santos C,Pereira GJ,Barbosa CM,Jurkiewicz A,Smaili SS,Bincoletto C

doi

10.1007/s00432-014-1640-4

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

909-20

issue

6

eissn

0171-5216

issn

1432-1335

journal_volume

140

pub_type

杂志文章
  • A multi-institutional study of clinicopathological features and molecular epidemiology of epidermal growth factor receptor mutations in lung cancer patients living with human immunodeficiency virus infection.

    abstract:PURPOSE:Lung cancer has become a crucial problem among individuals living with the human immunodeficiency virus (HIV) and causes high mortality in Western countries. Japan has an increasing number of newly infected HIV patients, and lung cancer is becoming a theme in this population. However, clinical factors of this p...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章,多中心研究

    doi:10.1007/s00432-015-1956-8

    authors: Okuma Y,Tanuma J,Kamiryo H,Kojima Y,Yotsumoto M,Ajisawa A,Uehira T,Nagai H,Takeda Y,Setoguchi Y,Okada S

    更新日期:2015-09-01 00:00:00

  • Phospho-mTOR and phospho-4EBP1 in endometrial adenocarcinoma: association with stage and grade in vivo and link with response to rapamycin treatment in vitro.

    abstract:PURPOSE:Endometrial adenocarcinoma, due to a frequent activation of PI3 K/AKT has been proposed as a candidate neoplasm for the treatment with mTOR inhibitors. Yet, data on the expression of mTOR cascade components in endometrial cancer are lacking. METHODS:To provide a basis for futher studies with mTOR inhibitors, w...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-008-0529-5

    authors: Darb-Esfahani S,Faggad A,Noske A,Weichert W,Buckendahl AC,Müller B,Budczies J,Röske A,Dietel M,Denkert C

    更新日期:2009-07-01 00:00:00

  • IL-6 signaling contributes to radioresistance of prostate cancer through key DNA repair-associated molecules ATM, ATR, and BRCA 1/2.

    abstract:PURPOSE:To study an association between IL-6 signaling and resistance to radiotherapy of prostate cancer (PCa) and explore the molecular mechanisms involved. METHODS:IL-6 expressing C4-2 and CWR22Rv1 (C4-2IL-6/CWRIL-6) and vector control (C4-2vec/CWRvec) cell lines were developed. Radiation-sensitivities of these cell...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-019-02917-z

    authors: Chen X,Chen F,Ren Y,Weng G,Xu L,Xue X,Keng PC,Lee SO,Chen Y

    更新日期:2019-06-01 00:00:00

  • Attitudes of members of the German Society for Palliative Medicine toward complementary and alternative medicine for cancer patients.

    abstract:BACKGROUND:A high proportion of cancer patients use complementary and alternative medicine (CAM). In oncology, risks of CAM are side effects and interactions. OBJECTIVE:Our aim was to conduct a survey on professionals in palliative care regarding attitudes toward CAM. DESIGN AND SUBJECTS:An internet-based survey with...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-014-1656-9

    authors: Conrad AC,Muenstedt K,Micke O,Prott FJ,Muecke R,Huebner J

    更新日期:2014-07-01 00:00:00

  • Down-regulation of a gastric transcription factor, Sox2, and ectopic expression of intestinal homeobox genes, Cdx1 and Cdx2: inverse correlation during progression from gastric/intestinal-mixed to complete intestinal metaplasia.

    abstract:PURPOSE:The molecular mechanisms underlying the development of intestinal metaplasia (IM) of the human stomach have yet to be clarified in detail. Besides ectopic expression of intestinal transcription factors, Cdx1 and Cdx2, little information is available regarding other regulatory factors. Hence, we here analyzed So...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-003-0519-6

    authors: Tsukamoto T,Inada K,Tanaka H,Mizoshita T,Mihara M,Ushijima T,Yamamura Y,Nakamura S,Tatematsu M

    更新日期:2004-03-01 00:00:00

  • Immunohistochemical and biochemical characterization of antisera raised to human tumor nucleoli.

    abstract::Antinucleolar antisera were raised in rabbits, goats and sheep to nucleoli isolated from three human tumor cell lines. The antisera were shown to cross-react by immunofluorescence with human tumor cell lines originating from different organs and with frozen sections from a wide variety of human malignant and non-malig...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/BF00400962

    authors: Greenfield RS,Lamon MO,Olech LJ,Grattan JA

    更新日期:1989-01-01 00:00:00

  • Molecular mechanisms of indirubin and its derivatives: novel anticancer molecules with their origin in traditional Chinese phytomedicine.

    abstract::Indirubin, a 3, 2' bisindole isomer of indigo, has originally been identified as the active principle of a traditional Chinese preparation and has been proven to exhibit antileukemic effectiveness in chronic myelocytic leukemia. Indirubin was detected to represent a novel lead structure with potent inhibitory potentia...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章,评审

    doi:10.1007/s00432-004-0579-2

    authors: Eisenbrand G,Hippe F,Jakobs S,Muehlbeyer S

    更新日期:2004-11-01 00:00:00

  • Interferon gamma and tumour necrosis factor alpha induce a synergistic antiproliferative response in human Ewing's sarcoma cells in vitro.

    abstract::Three human cell lines derived from Ewing's sarcoma (RM-82, VH-64, and WE-68) were investigated to establish the influence of recombinant human interferon gamma (rhIFN gamma) and tumour necrosis factor alpha (rhTNF alpha) on cell proliferation and survival and to characterize IFN gamma and TNF alpha receptor expressio...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/BF01372725

    authors: van Valen F,Winkelmann W,Burdach S,Göbel U,Jürgens H

    更新日期:1993-01-01 00:00:00

  • Variables associated with patient-reported outcomes in persons with chronic myeloid leukemia receiving tyrosine kinase-inhibitor therapy.

    abstract:PURPOSE:Explore patient-reported outcomes (PROs), including health-related quality of life (HRQoL), satisfaction with therapy, impact of the therapy on work and daily life, and concerns related to the therapy and identify variables associated with PROs in persons with chronic myeloid leukemia (CML) receiving tyrosine k...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-017-2353-2

    authors: Jiang Q,Wang HB,Yu L,Gale RP

    更新日期:2017-06-01 00:00:00

  • Induction of antigen-specific immune responses by dendritic cells transduced with a recombinant lentiviral vector encoding MAGE-A3 gene.

    abstract:PURPOSE:Melanoma antigen gene A3 (MAGE-A3) is aberrantly expressed in a number of cancer types. Because of its high specificity, MAGE-A3 has shown to be a promising candidate for cancer immunotherapy. Dendritic cells (DCs) have emerged as the natural agents for antigen delivery. DCs transduced with antigen may increase...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-013-1552-8

    authors: Lin L,Wei J,Chen Y,Huang A,Li KK,Zhang W

    更新日期:2014-02-01 00:00:00

  • Circulating tumor cells: advances in detection methods, biological issues, and clinical relevance.

    abstract:BACKGROUND:Circulating tumor cells (CTCs) have long been considered a reflection of tumor aggressiveness. Hematogenous spreading of CTCs from a primary tumor is a crucial step in the metastasis cascade, which leads ultimately to the formation of overt metastases. However, owing to the rarity of CTCs in peripheral blood...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章,评审

    doi:10.1007/s00432-011-0988-y

    authors: Sun YF,Yang XR,Zhou J,Qiu SJ,Fan J,Xu Y

    更新日期:2011-08-01 00:00:00

  • Transformation potential of bone marrow stromal cells into undifferentiated high-grade pleomorphic sarcoma.

    abstract:PURPOSE:Bone marrow adherent cells contain conventional bone marrow stromal cells and mesenchymal stem cells and these cells constitute the hematopoietic microenvironment. Mesenchymal stem cells have the capacity to give rise to multiple mesenchymal lineage cells and even ectodermal lineage cells. In the present study,...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-009-0723-0

    authors: Li Q,Hisha H,Takaki T,Adachi Y,Li M,Song C,Feng W,Okazaki S,Mizokami T,Kato J,Inaba M,Hosaka N,Maki M,Ikehara S

    更新日期:2010-06-01 00:00:00

  • The role of insulin-like growth factor I and its receptor in cell growth, transformation, apoptosis, and chemoresistance in solid tumors.

    abstract::Insulin-like growth factor I (IGF-I) exerts pleiotropic effects on mammalian cells via stimulation of its receptor (IGF-IR), a receptor tyrosine kinase. In vivo, IGF-I acts both as a local tissue growth factor and as a circulating hormone. In oncological research, IGF-I has received increased attention as the activate...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章,评审

    doi:10.1007/s004320050259

    authors: Grothey A,Voigt W,Schöber C,Müller T,Dempke W,Schmoll HJ

    更新日期:1999-01-01 00:00:00

  • Endometrial sampling devices for early diagnosis of endometrial lesions.

    abstract:PURPOSE:Endometrial carcinoma is the most common gynecologic malignancy in both developed and some developing countries. Unlike cervical cancer, for which there is routine screening, only patients symptomatic for endometrial carcinoma typically seek medical help for its diagnosis and treatment. Dilatation and curettage...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章,评审

    doi:10.1007/s00432-016-2215-3

    authors: Du J,Li Y,Lv S,Wang Q,Sun C,Dong X,He M,Ulain Q,Yuan Y,Tuo X,Batchu N,Song Q,Li Q

    更新日期:2016-12-01 00:00:00

  • Loss of allelic heterozygosity at the harvey ras locus in human oral carcinomas.

    abstract::The Harvey ras locus was examined for restriction fragment polymorphism and loss of allelic heterozygosity in 62 oral cancer patients. Southern blot analysis on BamHI digests of the tumour tissue DNA, revealed 23 patients with H-ras-1 heterozygosity. The probes used to study the polymorphism were the BamHI 6.6-kb frag...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/BF01612771

    authors: Saranath D,Bhoite LT,Mehta AR,Sanghavi V,Deo MG

    更新日期:1991-01-01 00:00:00

  • Positive expression of E-cadherin suppresses cell adhesion to fibronectin via reduction of alpha5beta1 integrin in human breast carcinoma cells.

    abstract::E-cadherin mainly mediated the epithelial cell-cell adhesion, and integrin signaling can modulate the signaling pathway of E-cadherin in the different levels. Up to now, however, it is still unclear that whether E-cadherin could interfere with cell-matrix interaction, a typical adhesion through integrins. In this stud...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-006-0128-2

    authors: Wu H,Liang YL,Li Z,Jin J,Zhang W,Duan L,Zha X

    更新日期:2006-12-01 00:00:00

  • H2-antagonists and carmustine.

    abstract::The highly metabolized nitrosourea carmustine (BCNU) is an anticancer agent which alkylates DNA and is metabolized to both active and inactive species by cytochrome P-450 enzymes. Other highly metabolized anticancer drugs have altered toxicities when some histamine H2 antagonists are coadministered. To test this hypot...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/BF00391598

    authors: Dorr RT,Soble MJ

    更新日期:1989-01-01 00:00:00

  • p53 Gene status in relation to ex vivo chemosensitivity of non-small cell lung cancer.

    abstract:PURPOSE:About 40% of non-small cell lung cancer (NSCLC) patients are candidates for systemic chemotherapy, despite the fact that at diagnosis most NSCLC are usually chemoresistant both in vivo and ex vivo. It is important to develop sufficient methods of prediction of the response to chemotherapy and to find molecular ...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-001-0305-2

    authors: Vogt U,Zaczek A,Klinke F,Granetzny A,Bielawski K,Falkiewicz B

    更新日期:2002-03-01 00:00:00

  • Estrogen receptor β potentiates the antiproliferative effect of raloxifene and affects the cell migration and invasion in HCT-116 colon cancer cells.

    abstract:BACKGROUND:Estrogen receptor β (ERβ) is the predominant ER in the colorectal epithelium, whose expression is greatly reduced in colorectal cancer compared with normal colon tissue. Recent in vitro studies suggested that ERβ may suppress tumor growth. No research was reported whether ERβ can be used as therapeutic agent...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-011-1145-3

    authors: Tu Z,Ma Y,Tian J,Li H,Akers W,Achilefu S,Gu Y

    更新日期:2012-07-01 00:00:00

  • Screening and detection of portal vein tumor thrombi-associated serum low molecular weight protein biomarkers in human hepatocellular carcinoma.

    abstract:PURPOSE:Serum low molecular weight protein biomarkers might be important in relation to portal vein tumor thrombi (PVTT) in hepatocellular carcinoma (HCC). This study aimed to screen and to detect these biomarkers. METHODS:We selected sera of 3 groups from 12 healthy volunteers, 12 HCC patients without PVTT and 12 HCC...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-007-0236-7

    authors: Qiu JG,Fan J,Liu YK,Zhou J,Dai Z,Huang C,Tang ZY

    更新日期:2008-03-01 00:00:00

  • Carcinogenicity of vinylethylnitrosamine in Syrian golden hamsters.

    abstract::Syrian golden hamsters received weekly s.c. doses of 5.0, 2.5, 1.25 or 0.63 mg/kg b.w. vinylethylnitrosamine (VEN). As dose levels increased, average survival time decreased, tumor multiplicity increased as did the total tumor rate which was between 50% and 100%. The main target of VEN effect was the respiratory tract...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/BF00411342

    authors: Green U,Althoff J

    更新日期:1982-01-01 00:00:00

  • Reversal of multidrug resistance by novel cyclosporin A analogues and the cyclopeptolide SDZ 214-103 biosynthesized in vitro.

    abstract::It was shown that cyclopeptolide SDZ 214-103 (10 microM) is more active in rhodamine-123 accumulation in actinomycin-D-resistant human lymphoma cells CCRF/ACTD400 than cyclosporin A (10 microM), but equipotent in the doxorubicin-resistant Friend erythroleukemia cell line F4-6/ADR. In F4-6/ADR cells, the 3-(4,5-dimethy...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/BF01212947

    authors: Schwabe K,Steinheider G,Lawen A,Traber R,Hildebrandt A

    更新日期:1995-01-01 00:00:00

  • Relevance of cellular and serum carbonic anhydrase IX in primary breast cancer.

    abstract:BACKGROUND:Carbonic anhydrase IX (CAIX) is involved in pH homeostasis, growth and survival of tumor cells. Besides the membranous form of CAIX, a soluble form is detectable in serum (s-CAIX). Overexpression of CAIX in tumors offers the opportunity for therapeutic strategies such as CAIX targeting antibodies. The aim of...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-013-1378-4

    authors: Schütze D,Milde-Langosch K,Witzel I,Rody A,Karn T,Schmidt M,Choschzick M,Jänicke F,Müller V

    更新日期:2013-05-01 00:00:00

  • Multiple functions of HuR in urinary tumors.

    abstract:PURPOSE:Human embryonic lethal abnormal visual-like protein, HuR, belongs to a member of the Hu family of RNA-binding protein and plays a critical role in urinary tumors. The purpose of this review is to summarize the current literature to demonstrate the importance of HuR in urinary tract tumors' biology and explore t...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章,评审

    doi:10.1007/s00432-018-2778-2

    authors: Zhang F,Cai Z,Lv H,Li W,Liang M,Wei X,Zhou F

    更新日期:2019-01-01 00:00:00

  • Circulating miR-125b is a novel biomarker for screening non-small-cell lung cancer and predicts poor prognosis.

    abstract:PURPOSE:MicroRNAs are small, non-coding RNAs that are critical regulators of various diseases including cancer, and may represent a novel class of cancer biomarkers. Recent reports have highlighted the oncogenic aspects of miR-125b. However, the level and clinical relevance of circulating miR-125b transcripts in human ...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-012-1285-0

    authors: Yuxia M,Zhennan T,Wei Z

    更新日期:2012-12-01 00:00:00

  • The effect of antibody against vascular endothelial growth factor on tumor growth and metastasis.

    abstract::Vascular endothelial growth factor (VEGF), a very important in the process of tumor angiogenesis, was chosen as a target in a study to determine whether manipulation of angiogenesis with antibody against VEGF may interrupt tumor growth and metastasis. Anti-VEGF antibody was obtained from immunized rabbits, purified on...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s004320050223

    authors: Wang G,Dong Z,Xu G,Yang Z,Shou C,Wang N,Liu T

    更新日期:1998-01-01 00:00:00

  • Growth-promoting effect of muscarinic acetylcholine receptors in colon cancer cells.

    abstract:PURPOSE:G-protein-coupled receptors are known to mediate cell growth via divergent signaling pathways. It has been reported that colon cancer cells express muscarinic acetylcholine receptor (mAChR) although their functional role is largely unknown. The aim of this study is to elucidate possible mechanisms responsible f...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-003-0433-y

    authors: Ukegawa JI,Takeuchi Y,Kusayanagi S,Mitamura K

    更新日期:2003-05-01 00:00:00

  • Overexpression of Cks1 is associated with poor survival by inhibiting apoptosis in breast cancer.

    abstract:PURPOSE:The present study was undertaken to examine the amplification and expression status of Cks1 in breast cancer and its significance. METHODS:The amplification and expression status of Cks1 gene was examined by FISH, real-time PCR and immunohistochemistry, respectively. RNA interference was used to detect the eff...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章,收录出版

    doi:10.1007/s00432-009-0582-8

    authors: Wang XC,Tian LL,Tian J,Wu HL,Meng AM

    更新日期:2009-10-01 00:00:00

  • Induced pluripotent cancer cells: progress and application.

    abstract::Induced pluripotent stem (iPS) cells are a group of pluripotent stem cells artificially derived from non-pluripotent cells typically by a forced expression of specific transcription factors. Generation of cancer-specific iPS cells, also called induced pluripotent cancer (iPC) cells, provides valuable experimental plat...

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章,评审

    doi:10.1007/s00432-010-0955-z

    authors: Sun C,Liu YK

    更新日期:2011-01-01 00:00:00

  • Expression of the Wnt antagonist DKK3 is frequently suppressed in sporadic epithelial ovarian cancer.

    abstract:PURPOSE:The Wnt pathway plays an important role in embryonic development, and defects in this pathway have been implicated in the tumorigenesis. The Dickkopf 3 (DKK3) is a putative Wnt signaling inhibitor that is frequently inactivated in human cancers. However, the expression of DKK3 in ovarian cancer remains unknown....

    journal_title:Journal of cancer research and clinical oncology

    pub_type: 杂志文章

    doi:10.1007/s00432-010-0916-6

    authors: You A,Fokas E,Wang LF,He H,Kleb B,Niederacher D,Engenhart-Cabillic R,An HX

    更新日期:2011-04-01 00:00:00