Quantitative comparison of rapamycin and cyclosporine effects on cytokine gene expression studied by reverse transcriptase-competitive polymerase chain reaction.

Abstract:

:We developed and applied a quantitative competitive polymerase chain reaction method to study the expression of various cytokine genes in Con A-stimulated murine splenocytes. This method relies on the use of competitive templates that differ from the target cytokine cDNA templates only by the introduction of a unique restriction endonuclease site in the center of each competitive template. After same-tube amplification using a single pair of oligonucleotide primers for both the target and competitive templates, restriction with the unique endonuclease yields 2 species that are readily resolved on agarose gel electrophoresis: full-length target and half-length competitor fragments. Using this method, we studied the time course and effects of CsA and rapamycin on IL-2, IFN-gamma, IL-4, and IL-10 gene expression following Con A stimulation. IL-2, IFN-gamma, and IL-10 share a common pattern of gene expression peaking at approximately 6 hr, while IL-4 gene expression peaks later, at approximately 20 hr. CsA very effectively inhibits expression of IL-2, IFN-gamma, and IL-4, but it inhibits IL-10 expression only 65%. Rapamycin inhibits IL-10 gene expression 100% and is less effective inhibiting the other cytokines. This pattern of inhibition is consistent with a calcium and protein kinase C independent pathway for IL-10 gene regulation and supports the notion that CsA and rapamycin may be used together to advantage.

journal_name

Transplantation

journal_title

Transplantation

authors

Zheng XX,Strom TB,Steele AW

subject

Has Abstract

pub_date

1994-07-15 00:00:00

pages

87-92

issue

1

eissn

0041-1337

issn

1534-6080

journal_volume

58

pub_type

杂志文章
  • Clinical and economic impact of flow cytometry crossmatching in primary cadaveric kidney and simultaneous pancreas-kidney transplant recipients.

    abstract::We retrospectively compared the clinical and financial impact of a final cross-match by T cell flow cytometry (FXM) versus conventional complement-dependent cytotoxicity (CXM) in consecutive primary cadaveric kidney (K) and primary simultaneous cadaveric pancreas-kidney (SPK) transplant recipients. Mean follow-up was ...

    journal_title:Transplantation

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    doi:10.1097/00007890-199706150-00018

    authors: Pelletier RP,Orosz CG,Adams PW,Bumgardner GL,Davies EA,Elkhammas EA,Henry ML,Ferguson RM

    更新日期:1997-06-15 00:00:00

  • Reasons for long-term use of steroid in primary adult liver transplantation under tacrolimus.

    abstract:BACKGROUND:Tacrolimus is a potent immunosuppressive agent that provides higher freedom from acute and chronic rejection than cyclosporine after liver transplantation (LTx). Initially, a steroid-free state was observed in about 70% of patients at 1 year; this did not change over the next 5 years. The present study ident...

    journal_title:Transplantation

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    doi:10.1097/00007890-200104270-00016

    authors: Jain A,Kashyap R,Marsh W,Rohal S,Khanna A,Fung JJ

    更新日期:2001-04-27 00:00:00

  • Evaluation and clinical significance of circulating immune complexes after renal transplantation.

    abstract::Using C1q binding, Kg binding, and Raji cell radioimmunoassays, the relationship between renal allograft rejection and the circulating immune complexes was examined in 90 patients with renal transplants. The data showed that there was a significant quantitative correlation between C1q-binding and Raji-cell-binding act...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-198308000-00009

    authors: Fukuda M

    更新日期:1983-08-01 00:00:00

  • What clinical alternatives to whole liver transplantation? Current status of artificial devices and hepatocyte transplantation.

    abstract::Shortage of organ donors limits the number of possible liver transplantations. Alternative therapies for treatment of liver failure are currently being developed: (i) extracorporeal artificial liver devices; (ii) bioartificial liver devices using hepatocytes; and (iii) hepatocyte transplantation. The objective of thes...

    journal_title:Transplantation

    pub_type: 社论,评审

    doi:10.1097/TP.0b013e3181963ad3

    authors: Sgroi A,Serre-Beinier V,Morel P,Bühler L

    更新日期:2009-02-27 00:00:00

  • The presence of HLA-directed antibodies after heart transplantation is associated with poor allograft outcome.

    abstract:BACKGROUND:The clinical significance of HLA-directed antibodies newly detected after transplantation (HT) is controversial. METHODS:Seventy-one HT recipients consented to enroll. Mean follow-up time was 28 months (range 6-48). Panel reactive antibody (PRA) analysis was performed on posttransplant sera (2 weeks, 1, 2, ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.tp.0000180564.14050.49

    authors: Tambur AR,Pamboukian SV,Costanzo MR,Herrera ND,Dunlap S,Montpetit M,Heroux A

    更新日期:2005-10-27 00:00:00

  • TNF-alpha signaling is not required for in vivo transcriptional reactivation of latent murine cytomegalovirus.

    abstract:BACKGROUND:Reactivation of cytomegalovirus (CMV) is frequently observed in recipients of solid organs and bone marrow transplants and is associated with increased risk of acute and chronic allograft rejection, opportunistic infection, graft failure, and patient mortality. The molecular mechanisms by which reactivation ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e3181b242a7

    authors: Zhang Z,Li Z,Yan S,Wang X,Abecassis M

    更新日期:2009-09-15 00:00:00

  • Financial Feasibility Analysis of a Culturally and Linguistically Competent Hispanic Kidney Transplant Program.

    abstract:BACKGROUND:In 2006, Northwestern Medicine implemented a culturally targeted and linguistically congruent Hispanic Kidney Transplant Program (HKTP). The HKTP has been associated with a reduction in Hispanic/Latino disparities in live donor kidney transplantation. This paper assessed the financial feasibility of implemen...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0000000000003269

    authors: Wang A,Caicedo JC,McNatt G,Abecassis M,Gordon EJ

    更新日期:2020-04-09 00:00:00

  • Characterization of immunoreactive trypsin as a means of differentiating graft pancreatitis and allograft rejection after porcine pancreatic transplantation.

    abstract::Graft pancreatitis and allograft rejection were both accompanied by increased serum levels of immunoreactive anionic trypsin (irAT) in a porcine pancreatic allograft transplantation model. Characterization of this immunoreactivity by gel filtration revealed different elution profiles in these conditions that can be he...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199201000-00004

    authors: Källén R,Borgström A

    更新日期:1992-01-01 00:00:00

  • Heart rate after cardiac transplantation-lessons from the tortoise and the shrew.

    abstract::There is a striking consistency in the total number of heart beats accrued over a lifetime across a range of animal species despite vast differences in size. Moreover, an inverse relationship is observed between heart rate and lifespan, leading to speculation that elevated heart rate could significantly affect longevi...

    journal_title:Transplantation

    pub_type: 社论,评审

    doi:10.1097/TP.0b013e31826bc42a

    authors: Critchley WR,Yonan N,Shaw SM,Fildes JE

    更新日期:2013-01-27 00:00:00

  • Extrinsic sympathetic reinnervation after intestinal transplantation in rats.

    abstract::Although extrinsic denervation is inevitable after intestinal transplantation and leads to poor intestinal function, little is known about the occurrence of extrinsic reinnervation. In this study, extrinsic sympathetic reinnervation was investigated morphologically following syngeneic intestinal transplantation perfor...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:

    authors: Kiyochi H,Ono A,Yamamoto N,Ohnishi K,Shimahara Y,Kobayashi N

    更新日期:1995-02-15 00:00:00

  • Kidney transplantation and the intensity of poverty in the contiguous United States.

    abstract:BACKGROUND:Geographic variation in kidney transplantation rates in the United States has been described previously but remains unexplained by age, race, sex, or socioeconomic status differences. Geographic variations in the concentration of poverty appear to impact end-stage renal disease care and potentially access to...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0000000000000125

    authors: Mohan S,Mutell R,Patzer RE,Holt J,Cohen D,McClellan W

    更新日期:2014-09-27 00:00:00

  • Alloantibodies against donor epidermis and early kidney transplant rejection.

    abstract::We have previously observed that transplant recipient sera with endothelial antibodies are bound to epidermal cells, as shown by immunofluorescence on sections of skin. It was also reported that early kidney failures that occurred despite negative T cell crossmatches were associated with, and could have been predicted...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199102000-00019

    authors: Moraes JR,Moraes ME,Luo YM,Stastny P

    更新日期:1991-02-01 00:00:00

  • Induction of delayed hypersensitivity to alloantigens coinjected with Langerhans cells into the anterior chamber of the eye. Abrogation of anterior chamber-associated immune deviation.

    abstract::Inoculation of P815 tumor cells (DBA/2 origin) into the anterior chamber of eyes of BALB/c mice normally produces anterior chamber-associated immune deviation (ACAID) whereby delayed hypersensitivity (DH) responses to the minor H alloantigens of the tumor cells are suppressed. Based on our previous work showing an ass...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-198903000-00024

    authors: Williamson JS,Streilein JW

    更新日期:1989-03-01 00:00:00

  • Use of alemtuzumab and tacrolimus monotherapy for cadaveric liver transplantation: with particular reference to hepatitis C virus.

    abstract::We have proposed that the mechanisms of alloengraftment and variable acquired tolerance can be facilitated by minimum posttransplant immunosuppression. It was further suggested that the efficacy of minimalistic treatment could be enhanced by preoperative recipient conditioning with an antilymphoid antibody preparation...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.tp.0000142674.78268.01

    authors: Marcos A,Eghtesad B,Fung JJ,Fontes P,Patel K,Devera M,Marsh W,Gayowski T,Demetris AJ,Gray EA,Flynn B,Zeevi A,Murase N,Starzl TE

    更新日期:2004-10-15 00:00:00

  • Donor-specific skin transplants activate allodestructive T cells in mice resistant to neonatal H-2 tolerance induction.

    abstract::Mice of the B10 background that are class II I-E nonexpressing demonstrate a relative resistance to neonatal induction of tolerance of class I alloantigens from I-E-expressing B10 strains. The majority of these injected mice delete donor-responsive and "I-E-reactive" (V beta 11+) cells in the immediate postinoculation...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199312000-00035

    authors: Nassiri M,Viciana A,Streilein JW,Ruiz P

    更新日期:1993-12-01 00:00:00

  • Blood transfusions in liver recipients: a conundrum or a clear benefit in the cyclosporine/tacrolimus era?

    abstract::Blood transfusions are common in patients with end-stage liver disease (ESLD), and their effects on sensitization, rejection, and liver graft survival are not well known. These effects were examined in 121 recipients of primary liver grafts, surviving > or = 30 days. Ninety-six (79%) patients received transfusions bef...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199706150-00008

    authors: Koneru B,Harrison D,Rizwan M,Holland BK,Ippolito T,Holman MJ,Leevy CB

    更新日期:1997-06-15 00:00:00

  • Disseminated varicella infection in adult renal allograft recipients: four cases and a review of the literature.

    abstract::Disseminated varicella-zoster (VZV) infection is a rare complication after renal allotransplantation in adults. We report four patients, among them one with combined VZV and cytomegalovirus infection. The main complications were hepatitis, pneumonitis, and disseminated intravascular coagulation. A review of the litera...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200202270-00023

    authors: Fehr T,Bossart W,Wahl C,Binswanger U

    更新日期:2002-02-27 00:00:00

  • Enzyme release from injured, preserved, and ex vivo reperfused liver does not indicate malfunction.

    abstract:OBJECTIVE:We compared the enzyme release from preserved and ex vivo reperfused livers after acute injury or inflammatory stimulus with organ function. METHODS:Acute injury was induced by carbon tetrachloride and inflammation was induced by turpentine oil treatments. Livers were exsanguinated and preserved for 8 or 24 ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200210270-00004

    authors: Gioli-Pereira L,Coradin K,Nagaoka MR,Borges DR,Kouyoumdjian M

    更新日期:2002-10-27 00:00:00

  • A comparison of gene expression in murine cardiac allografts and isografts by means DNA microarray analysis.

    abstract:BACKGROUND:Acute rejection of allografts remains a significant problem in clinical transplantation, and the fundamental mechanism underlying this rejection are as yet only poorly elucidated. Recently, DNA microarrays have come into use for the study of gene expression profiles, and we have taken advantage of this new t...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200107270-00027

    authors: Saiura A,Mataki C,Murakami T,Umetani M,Wada Y,Kohro T,Aburatani H,Harihara Y,Hamakubo T,Yamaguchi T,Hasegawa G,Naito M,Makuuchi M,Kodama T

    更新日期:2001-07-27 00:00:00

  • Islet graft survival and function: concomitant culture and transplantation with vascular endothelial cells in diabetic rats.

    abstract:BACKGROUND:Human islet transplantation is a great potential therapy for type I diabetes. To investigate islet graft survival and function, we recently showed the improved effects after co-culture and co-transplantation with vascular endothelial cells (ECs) in diabetic rats. METHODS:ECs were isolated, and the viability...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e3182356ca7

    authors: Pan X,Xue W,Li Y,Feng X,Tian X,Ding C

    更新日期:2011-12-15 00:00:00

  • Novel antibodies to the donor stem cell population CD34+/VEGFR-2+ are associated with rejection after hematopoietic stem cell transplantation.

    abstract:BACKGROUND:Reconstitution of hematopoiesis after hematopoietic stem cell transplantation (HSCT) occurs through a reservoir of donor CD34+ HSC. CD34+/VEGFR-2+ is a primitive, quiescent subpopulation of HSC known to generate hematopoietic or endothelial progeny in vitro and in vivo. We hypothesized that donor-specific an...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e3181820333

    authors: Nordlander A,Mattsson J,Sundberg B,Sumitran-Holgersson S

    更新日期:2008-09-15 00:00:00

  • Modulation of accelerated rejection of cardiac allografts in sensitized rats by anti-interleukin 2 receptor monoclonal antibody and cyclosporine therapy.

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    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199102000-00004

    authors: Kupiec-Weglinski JW,Sablinski T,Hancock WW,Di Stefano R,Mariani G,Mix CT,Tilney NL

    更新日期:1991-02-01 00:00:00

  • Impact of disparity of minor histocompatibility antigens HA-1, CD31, and CD49b in hematopoietic stem cell transplantation of patients with chronic myeloid leukemia with sibling and unrelated donors.

    abstract::Despite human leukocyte antigen (HLA) identity between donor and recipient, several patients develop acute graft-versus-host disease (aGVHD) after hematopoetic stem cell transplantation (HSCT) because of minor histocompatibility antigen (mHag) incompatibilities. The impact of multiple mHag disparities on the clinical ...

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    pub_type: 杂志文章

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    authors: Heinemann FM,Ferencik S,Ottinger HD,Beelen DW,Grosse-Wilde H

    更新日期:2004-04-15 00:00:00

  • Conversion from cyclosporine to azathioprine in renal allograft recipients.

    abstract::Fifty seven recipients of renal allografts initially treated with CsA and low-dose prednisone were switched to azathioprine and low-dose steroids. Ten had prolonged (greater than 28 days) allograft nonfunction after transplantation (group 1), 8 had ongoing, poorly controlled rejection (group 2), and 39 had stable func...

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    pub_type: 杂志文章

    doi:10.1097/00007890-198412000-00024

    authors: Rocher LL,Milford EL,Kirkman RL,Carpenter CB,Strom TB,Tilney NL

    更新日期:1984-12-01 00:00:00

  • Anti-CD20 monoclonal antibody (rituximab) therapy for acute cardiac humoral rejection: a case report.

    abstract::Humoral or antibody-mediated rejection in cardiac transplant recipients is mediated by donor-specific cytotoxic antibodies and is histologically defined by linear deposits of immunoglobulin and complement in the myocardial capillaries. Antibody-mediated rejection often is accompanied by hemodynamic compromise and is a...

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    pub_type: 杂志文章

    doi:10.1097/00007890-200203270-00013

    authors: Aranda JM Jr,Scornik JC,Normann SJ,Lottenberg R,Schofield RS,Pauly DF,Miles M,Hill JA,Sleasman JW,Skoda-Smith S

    更新日期:2002-03-27 00:00:00

  • Attenuating Ischemia-Reperfusion Injury in Kidney Transplantation by Perfusing Donor Organs With siRNA Cocktail Solution.

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    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0000000000000960

    authors: Zheng X,Zang G,Jiang J,He W,Johnston NJ,Ling H,Chen R,Zhang X,Liu Y,Haig A,Luke P,Jevnikar AM,Min WP

    更新日期:2016-04-01 00:00:00

  • Acute tubular necrosis after renal transplantation.

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    doi:10.1097/00007890-198003000-00016

    authors: Brophy D,Najarian JS,Kjellstrand CM

    更新日期:1980-03-01 00:00:00

  • The effect of mycophenolate mofetil and azathioprine dose on renal allograft outcome in the United kingdom.

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    pub_type: 杂志文章

    doi:10.1097/TP.0b013e318186dc81

    authors: Shah S,Collett D,Johnson R,Raftery M,Rudge C,Yaqoob MM

    更新日期:2008-10-27 00:00:00

  • Branch patch reconstruction in living donor liver transplantation: arterialization of grafts with replaced type arteries.

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    pub_type: 杂志文章

    doi:10.1097/01.tp.0000236102.36326.a6

    authors: Aramaki O,Sugawara Y,Kokudo N,Takayama T,Makuuchi M

    更新日期:2006-12-15 00:00:00

  • Overall and cause-specific mortality in transplant recipients with a pretransplantation cancer history.

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    authors: Brattström C,Granath F,Edgren G,Smedby KE,Wilczek HE

    更新日期:2013-08-15 00:00:00