Abstract:
:1. We investigated the glycine-induced response in ventromedial hypothalamic (VMH) neurons freshly dissociated from 8- to 12-day-old rats using the nystatin and gramicidin perforated patch recording modes. The nystatin-formed pores in the plasma membrane are permeable for both monovalent cations and anions, whereas those formed by gramicidin are permeable only to monovalent cations. Therefore, when the patch-pipette contains 150 mM Cl- and gramicidin, the physiological intracellular Cl- concentration ([Cl-]i) is undisturbed in the cell-attached condition of the pipette. 2. At holding potentials of -40 to -60 mV, glycine induced inward currents and outward currents in the nystatin and gramicidin perforated patch recording modes, respectively. The values of the half-maximum effective concentration (EC50) and the Hill coefficient in the concentration-response relationships of the glycine responses were 2.9 x 10(-5) M, 1.1, and 4.2 x 10(-5) M, 1.4, respectively. These values were quite similar in both recording modes. 3. The reversal potentials of the glycine responses (EGly) were -1.5 mV in the nystatin perforated patch recording and -75.0 to -24.8 mV in the gramicidin perforated patch recording. 4. Strychnine (3 x 10(-8) M) inhibited the glycine-induced outward currents in a competitive manner and the half-inhibition concentration (IC50) of strychnine on the 10(-4) M glycine-induced response was 1.9 x 10(-8) M. 5. The physiological [Cl-]i in the VMH neurons calculated from the EGly obtained by the gramicidin perforated patch mode ranged from 6.0 to 43.8 mM (n = 28).
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Abe Y,Furukawa K,Itoyama Y,Akaike Ndoi
10.1152/jn.1994.72.4.1530subject
Has Abstractpub_date
1994-10-01 00:00:00pages
1530-7issue
4eissn
0022-3077issn
1522-1598journal_volume
72pub_type
杂志文章abstract::1. The horizontal eye movements induced by acceleration along the interaural axis were recorded from five monkeys (Macaca mulatta) by the use of the electromagnetic search-coil technique. Animals sat on a sled that was moved briefly in darkness along a linear track (bell-shaped acceleration profile: peak, 400 mm/s2; d...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1991.66.3.851
更新日期:1991-09-01 00:00:00
abstract::Recent work has implicated low-frequency (<20 Hz) neuronal phase information as important for both auditory (<10 Hz) and speech [theta (∼4-8 Hz)] perception. Activity on the timescale of theta corresponds linguistically to the average length of a syllable, suggesting that information within this range has consequences...
journal_title:Journal of neurophysiology
pub_type: 杂志文章,随机对照试验
doi:10.1152/jn.00075.2011
更新日期:2011-08-01 00:00:00
abstract::It is well established that orderly patterns of motor neuron activity, muscle recruitment, and limb movement are generated in chicks during motility by embryonic day (E)9, the midpoint in embryonic development. However, our recent work suggests that some attributes of motility, such as the rhythm of repetitive limb mo...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00393.2003
更新日期:2003-09-01 00:00:00
abstract::Single midbrain neurons were examined for effects of ovarian hormone administration on responsiveness to an array of lordosis-controlling types of somatosensory stimuli in anesthetized, ovariectomized Syrian hamsters. Neuronal responses were recorded under four different hormonal treatment conditions: 1) estradiol ben...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1984.51.5.1040
更新日期:1984-05-01 00:00:00
abstract::As a vital skill in an evolving world, interception of moving objects relies on accurate prediction of target motion. In natural circumstances, active gaze shifts often accompany hand movements when exploring targets of interest, but how eye and hand movements are coordinated during manual interception and their depen...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00476.2017
更新日期:2018-01-01 00:00:00
abstract::1. Spatiotemporal receptive fields (RFs) for left and right eyes were studied for simple cells in the cat's striate cortex to examine the idea that stereoscopic depth information is encoded via structural differences of RFs between the two eyes. Traditional models are based on neurons that possess matched RF profiles ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.75.5.1779
更新日期:1996-05-01 00:00:00
abstract::1. Monkeys were trained to react to an arm perturbation according to an instruction delivered prior to the perturbation. There were two possible instructions (push or pull), and monkeys learned to respond accordingly regardless of the direction (push or pull) of the triggering perturbation. 2. Pyramidal tract neurons ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1976.39.5.1069
更新日期:1976-09-01 00:00:00
abstract::Precise motion control is critical to human survival on Earth and in space. Motion sensation is inherently imprecise, and the functional implications of this imprecision are not well understood. We studied a "vestibular" manual control task in which subjects attempted to keep themselves upright with a rotational hand ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00565.2018
更新日期:2018-12-01 00:00:00
abstract::1. High-voltage-activated Ca2+ currents [ICa(HVA)] were studied in immunolabeled mouse retinal ganglion neurons (RGNs) to elucidate channel-specific components and their developmental changes in vitro. 2. Neurons were dissociated at postnatal day 5. RGNs were selected for electrophysiological measurements by vital lab...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1994.72.5.2542
更新日期:1994-11-01 00:00:00
abstract::The discharge patterns of single units in the ventral cochlear nucleus (VCN) of anesthetized guinea pigs were examined in response to iterated rippled noise (IRN) as a function of the IRN delay (which determines the IRN pitch) and the IRN sound level. Delays were varied over five octaves in half-octave steps, and soun...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2001.85.3.1206
更新日期:2001-03-01 00:00:00
abstract::Recent work in the anterior piriform cortex (aPCX) has demonstrated that cortical odor receptive fields are highly dynamic, showing rapid changes of both firing rate and temporal patterning within relatively few inhalations of an odor, despite relatively maintained, patterned input from olfactory bulb mitral/tufted ce...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2000.84.6.3036
更新日期:2000-12-01 00:00:00
abstract::Mechanosensory neurons exhibit a wide range of dynamic changes in response, including rapid and slow adaptation. In addition to mechanical factors, electrical processes may also contribute to sensory adaptation. We have investigated adaptation of afferent neurons in the stick insect femoral chordotonal organ (fCO). Th...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00339.2002
更新日期:2002-11-01 00:00:00
abstract::Sindbis viruses have been widely used in neurobiology to express a variety of genes in cultured neurons, in cultured slices, and in vivo. They provide fast onset and high levels of expression of foreign genes, but the expression is limited to a short time window due to a shut-off of host protein synthesis. We have use...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00464.2003
更新日期:2003-10-01 00:00:00
abstract::We performed a whole-body mapping study of the effect of unilateral muscle vibration, eliciting spindle Ia firing, on the control of standing and walking in humans. During quiet stance, vibration applied to various muscles of the trunk-neck system and of the lower limb elicited a significant tilt in whole body postura...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00764.2006
更新日期:2007-01-01 00:00:00
abstract::1. To obtain a comprehensive map of the ascending projection of the midbrain periaqueductal gray, small amounts of [3H]leucine were injected into discrete regions of the periaqueductal gray (PAG) of the monkey. 2. Despite the fact that different regions of the PAG were injected in separate animals, the majority of the...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1983.49.3.567
更新日期:1983-03-01 00:00:00
abstract::Spontaneous and stimulus-evoked excitatory postsynaptic currents (EPSCs) were recorded in calyx nerve terminals from the turtle vestibular lagena to quantify key attributes of quantal transmission at this synapse. On average, EPSC events had a magnitude of ∼ 42 pA, a rise time constant of τ(0) ∼ 229 μs, decayed to bas...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00055.2015
更新日期:2015-06-01 00:00:00
abstract::Targeting the melanin-concentrating hormone (MCH) system has been suggested as a potential treatment for obesity, anxiety disorders, as well as addiction. Despite the therapeutic potential of MCH agonists and antagonists, the endogenous factors regulating MCH activity, in particular those implicated in anxiety and rew...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00791.2010
更新日期:2011-03-01 00:00:00
abstract::The suppressive action of a moving textured background on responses to moving bars was investigated in 118 striate neurons, 19 dorsal lateral geniculate neurons, and 5 perigeniculate neurons in paralyzed and anesthetized cats. In standard conditions the background was a two-dimensional (2D) noise pattern, the bar move...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1987.57.6.1767
更新日期:1987-06-01 00:00:00
abstract::The function of the ventral nucleus of the lateral lemniscus (VNLL), a secondary processing site within the auditory brain stem, is unclear. It is known to be a major source of inhibition to the inferior colliculus (IC). It is also thought to play a role in coding the temporal aspects of sound, such as onsets and the ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00167.2005
更新日期:2005-08-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00955.2014
更新日期:2015-04-01 00:00:00
abstract::Ifenprodil is a selective blocker of NMDA receptors that are heterodimers composed of GluN1/GluN2B subunits. This pharmacological profile has been extensively used to test the role of GluN2B-containing NMDA receptors in learning and memory formation. However, ifenprodil has also been reported to have actions at a numb...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01066.2011
更新日期:2012-03-01 00:00:00
abstract::The continuous perception of motion-through-depth is critical for both navigation and interacting with objects in a dynamic three-dimensional (3D) world. Here we used 3D tracking to simultaneously assess the perception of motion in all directions, facilitating comparisons of responses to motion-through-depth to fronto...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00831.2016
更新日期:2017-09-01 00:00:00
abstract::Contrast thresholds for detection of stationary and flickering gratings were measured behaviorally for each eye of cats raised with induced convergent strabismus. The performance of the deviating eye was inferior to that of the nondeviating eye when test patterns were stationary. Flicker served to reduce the performan...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1983.50.1.287
更新日期:1983-07-01 00:00:00
abstract::1. We studied the effect of temporarily inhibiting neurons in the caudal fastigial nucleus in two rhesus macaques trained to make saccades to jumping targets. We placed injections of the gamma-aminobutyric acid (GABA) agonist muscimol unilaterally or bilaterally at sites in the caudal fastigial nucleus where we had re...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1993.70.5.1741
更新日期:1993-11-01 00:00:00
abstract::In addition to a primary somatosensory cortex (SI), the cerebral cortex of all mammals contains a second somatosensory area (SII); however, the functions of SII are largely unknown. Our aim was to explore the functions of SII by comparing response properties of whisker-related neurons in this area with their counterpa...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00262.2004
更新日期:2004-10-01 00:00:00
abstract::Memory for past events may be based on retrieval accompanied by specific contextual details (recollection) or on the feeling that an item is old (familiarity) or new (novelty) in the absence of contextual details. There are indications that recollection, familiarity, and novelty involve different medial temporal lobe ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01029.2005
更新日期:2006-10-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.91350.2008
更新日期:2009-12-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章,随机对照试验
doi:10.1152/jn.00728.2009
更新日期:2010-10-01 00:00:00
abstract::Low-intensity alternating electric fields applied to the scalp are capable of modulating cortical activity and brain functions, but the underlying mechanisms remain largely unknown. Here, we report two distinct components of voltage-sensitive dye signals induced by low-intensity, alternating electric fields in rodent ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00740.2013
更新日期:2014-11-15 00:00:00
abstract::We have used electrophysiological techniques to document segmental differences in the pathways between the giant, escape command axons, lateral giants (LG) and medial giants (MG), and the nongiant, fast flexor (FF) motoneurons. We found no difference in the input from LG and MG axons to FF motoneurons in the posterior...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1985.53.1.252
更新日期:1985-01-01 00:00:00