DNA assembly for plant biology: techniques and tools.

Abstract:

:As the speed and accuracy of genome sequencing improves, there are ever-increasing resources available for the design and construction of synthetic DNA parts. These can be used to engineer plant genomes to produce new functions or to elucidate the function of endogenous sequences. Until recently the assembly of amplified or cloned sequences into large and complex designs was a limiting step in plant synthetic biology and biotechnology. A number of new methods for assembling DNA molecules have been developed in the last few years, several of which have been applied to the production of molecules used to modify plant genomes.

journal_name

Curr Opin Plant Biol

authors

Patron NJ

doi

10.1016/j.pbi.2014.02.004

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

14-9

eissn

1369-5266

issn

1879-0356

pii

S1369-5266(14)00020-X

journal_volume

19

pub_type

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