Abstract：
BACKGROUND:Currently, behavioral research of aggressiveness is often conducted with intraspecific intermale aggression tests. Intraspecific aggression is not detectable in early stages of psychiatric disorders or in female animals, except during the nursing period. NEW METHOD:We developed a semi-automated apparatus (ARM: Aggression Response Meter) for measurement of aggressive biting behavior (ABB) in mice. The apparatus is loaded with computer-controlled sticks that stimulate the mouse through touch, inducing irritation and anger. When the mouse bites the sticks in anger, a load sensor attached to the sticks detects ABB dynamically. Changes in ABB were assessed with isolation-reared/re-socialized mice using the ARM, and additional isolation-reared mice were tested using both the ARM and the resident-intruder test, and then buspirone, a serotonin 1A receptor agonist, was administered. RESULTS:ABB significantly increased during isolation rearing, and then significantly decreased throughout the re-socialization period; both changes were time-dependent. The ARM also detected ABB of female mice after 3 weeks of isolation rearing. Buspirone significantly inhibited aggressive behavior in both tests in a similar manner. COMPARISON WITH EXISTING METHOD:The ARM detects aggressiveness in psychiatric disorders at an earlier stage and in both male and female mice. CONCLUSIONS:ABB toward inanimate objects is a reliable paradigm that makes it possible to detect aggressiveness in the early stage of psychiatric disorders. The ARM is useful for the quantification of aggressiveness using the same individual repeatedly, and for objective evaluation of the effects of drugs on aggressiveness. The ARM can be used with both male and female mice.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Kuchiiwa S,Kuchiiwa Tdoi
10.1016/j.jneumeth.2014.02.017subject
Has Abstractpub_date
2014-05-15 00:00:00pages
27-34eissn
0165-0270issn
1872-678Xpii
S0165-0270(14)00078-8journal_volume
228pub_type
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