Abstract：
:The O specific polysaccharide (OSP) of the lipopolysaccharide (LPS) of Salmonella enterica serovar Paratyphi A is a protective antigen and the target for vaccine development. LPS is the major constituent of the outer membrane of S. Paratyphi A with the OSP exposed on the surface, in addition to the cell associated LPS a large amount of free LPS was present in the fermentation broth. A purification method was developed to take advantage of both sources of LPS and to maximize recovery of OSP. After fermentation the bacterial cells were concentrated and washed, the permeate containing the free LPS was processed separately from the cells. The free LPS was concentrated and washed on a 100kD ultrafiltration membrane to remove low molecular weight impurities. The LPS was then detoxified by separation of the lipid A from the OSP using acid hydrolysis at 100°C, the precipitated lipid A was removed by 0.2μm membrane filtration. Contaminants were then removed by acid precipitation in the presence of sodium deoxycholate. The OSP was concentrated and washed with 1M NaCl then water using a 10kD ultrafiltration membrane then sterile filtered through a 0.2μm membrane filter. The cells were treated by acid hydrolysis at 100°C, the remaining cells, cell debris and precipitate was removed by centrifugation. The filtrate was then treated in the same way as described above for the free LPS. The combined yield of purified OSP from free LPS plus the cells was greater than 880mg/L of culture broth. The method developed yields large amounts of OSP, is scalable and compatible with cGMP so would be readily transferrable to developing country vaccine manufacturers for low cost production of vaccine against S. Paratyphi A.
journal_name
Vaccinejournal_title
Vaccineauthors
Kothari S,Kim JA,Kothari N,Jones C,Choe WS,Carbis Rdoi
10.1016/j.vaccine.2014.02.090subject
Has Abstractpub_date
2014-05-01 00:00:00pages
2457-62issue
21eissn
0264-410Xissn
1873-2518pii
S0264-410X(14)00317-Xjournal_volume
32pub_type
杂志文章相关文献
VACCINE文献大全abstract：:Vaccines that could protect humans against the highly lethal Marburg and Ebola viruses have eluded scientists for decades. Classical approaches have been generally unsuccessful for Marburg and Ebola viruses and pose enormous safety concerns as well. Modern approaches, in particular those using vector-based approaches ...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2006.11.037
更新日期：2007-03-01 00:00:00
abstract：:The distribution of alpha-casein, bovine serum albumin (BSA), myoglobin and recombinant protective antigen (rPA) in mono-valent and combination vaccines containing aluminum hydroxide adjuvant was studied by fluorescence microscopy and flow cytometry. Green and red fluorescent probes were conjugated to the antigens. Ad...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2003.10.040
更新日期：2004-05-07 00:00:00
abstract：:Although few diseases have had a greater impact on human history, currently there is no vaccine available for protection against plague that is licensed by the Food and Drug Administration (FDA). DynPort Vaccine Company LLC, a CSC company (DVC), is managing the advanced development of a recombinant plague vaccine (rF1...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.01.071
更新日期：2007-04-20 00:00:00
abstract：BACKGROUND:Although measles is a highly infectious disease, the live measles vaccine provides protection for over 20 years, and immunity may be lifelong. This study assessed measles seroprevalence in schoolchildren in the Cochabamba region of Bolivia. METHODS:A seroepidemiological survey of measles immunity in 5-16-ye...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2012.12.037
更新日期：2013-04-12 00:00:00
abstract：:Passive immunization of mice with 131 micrograms of the non-neutralizing monoclonal antibody (mAb) 18A2B2, directed against the A subgroup epitope of the G glycoprotein of respiratory syncytial virus Long strain (RSV), confers protection against viral i.n. challenge. The role of the Fc fragment of this antibody as wel...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(95)00222-m
更新日期：1996-04-01 00:00:00
abstract：:Vaccination is an effective strategy to prevent pneumococcal diseases. Currently, licensed vaccines include the pneumococcal polysaccharide vaccine (PPSV) and the pneumococcal conjugate vaccine (PCV), which target some of the most common of the 94 serotypes of S. pneumoniae based on their capsular composition. However...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2018.11.015
更新日期：2019-01-03 00:00:00
abstract：:Groups of calves (20 per group) were vaccinated subcutaneously with a single dose of BCG Pasteur (6 x 10(4) or 6 x 10(6) colony forming units) and two months later, 15 calves from each group were challenged intratracheally with virulent Mycobacterium bovis. Vaccination with either dose of BCG induced significant prote...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(94)00055-r
更新日期：1995-08-01 00:00:00
abstract：:In anticipation of future combination vaccines, a recombinant class 3 porin (rPorB) of group B meningococci was evaluated as an alternative carrier protein for a Haemophilus influenzae type b (Hib) polyribosylribotol phosphate (PRP) conjugate vaccine. The use of rPorB may avoid undesirable immunologic interactions amo...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(98)00174-1
更新日期：1998-11-01 00:00:00
abstract：:We previously demonstrated that mucosal immunization with SHIV virus-like particles (VLPs) was able to induce strong humoral and cellular immune responses against HIV envelope protein (Env). To understand the mechanism for such enhanced immune responses, we studied the interaction between VLPs and dendritic cells (DCs...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2004.05.036
更新日期：2004-11-25 00:00:00
abstract：BACKGROUND:Sci-B-Vac® is a tri-antigenic recombinant Hepatitis B vaccine (TAV) containing the small (s), medium (pre-S2) and large (pre-S1) hepatitis B surface (HBs) antigens. To comply with vaccine licensure, a new reference standard batch was qualified by characterizing the seroprotection rate (SPR) for anti-HBs tite...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2020.12.050
更新日期：2021-01-13 00:00:00
abstract：:The efficacy of a 10 or 20 micrograms antigen load of HB recombinant vaccines is still being debated. A comparison of anti-HBs titres in two groups of healthy subjects vaccinated by the same schedule (0, 1 and 6 months) employing recombinant HB vaccines with different antigen loads, 20 micrograms (group A, 251 subject...
journal_title：Vaccine
pub_type： 临床试验,杂志文章
doi：10.1016/0264-410x(95)00148-t
更新日期：1996-02-01 00:00:00
abstract：:To demonstrate the potential of pH-sensitive carbonate apatite (CO₃Ap) nanoparticles as DNA vaccine carriers to enhance vaccination efficacy, we examined the humoral and cellular immune responses of C57BL/6 mice immunized with the plasmid expression vector pCI-neo encoding the full-length soluble ovalbumin (OVA) (pCI-...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2014.09.032
更新日期：2014-10-29 00:00:00
abstract：:Flagellin of Pseudomonas aeruginosa is an important vaccine candidate. N-terminal domains are highly conserved in both type a and type b flagellins. The efficacy of gold nanoparticles (AuNPs) conjugated to N-terminal domains of P. aeruginosa flagellin (flagellin(1-161)), as an immunogen in mice, has been assessed. The...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2016.01.041
更新日期：2016-03-14 00:00:00
abstract：:We investigated whether pre-existing adenovirus-specific immunity influenced the development of immunity to a foreign antigen expressed by recombinant adenovirus. Active adenovirus-specific immunity was induced in cotton rats by i.n. administration of wild type human adenovirus type 5 (HAd5) two weeks before immunisat...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(98)00279-5
更新日期：1999-02-26 00:00:00
abstract：:This randomized, double-blind trial conducted in the US and Canada was conducted to compare the safety and immunogenicity of two dosages of live attenuated oral monovalent G1 human rotavirus (HRV) vaccine administered orally at a 2-month interval concomitantly with routine childhood vaccines to healthy infants 5-15 we...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2005.07.030
更新日期：2006-05-01 00:00:00
abstract：:Through the specific and tight interaction between streptavidin and biotin, a novel platform was developed to allow for rapid (less than 2h), efficient and durable display of streptavidin-tagged bioactive GM-CSF on the surface of biotinylated B16.F10 tumor cells. This technology involved biotinylation of the cell memb...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2006.04.031
更新日期：2006-06-19 00:00:00
abstract：:Human papillomaviruses (HPV), particularly HPV16, is considered a necessary cause of cervical and oral cancer. Thus, the development of a therapeutic vaccine against HPV is important for the control of cervical cancer. However, therapeutic vaccination has been limited by inadequate antigen-specific immune responses. H...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.12.034
更新日期：2008-03-04 00:00:00
abstract：:Bacterial infectious diseases remain the single most important threat to health worldwide. Although conventional vaccinology approaches were successful in conferring protection against several diseases, they failed to provide efficacious solutions against many others. The advent of whole-genome sequencing changed the ...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2009.01.072
更新日期：2009-05-26 00:00:00
abstract：:The global health community is beginning to gain an understanding of the global burden of norovirus-associated disease, which appears to have significant burden in both developed- and developing-country populations. Of particular importance is the growing recognition of norovirus as a leading cause of gastroenteritis ...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2016.03.077
更新日期：2016-06-03 00:00:00
abstract：:Systemic (spleen cell (SPLC), serum antibodies) and intestinal mucosal (Peyer's patch cells (PPC), lamina propria lymphocytes (LPLs), coproantibodies) immune responses were compared in mice immunized with varying doses (144, 72, 36, 18 ELISA units [EU]) of HAVRIX, an alum-adsorbed killed hepatitis A virus (HAV) vaccin...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(02)00699-0
更新日期：2003-03-28 00:00:00
abstract：:Endospores of the Gram-positive bacterium, Bacillus subtilis, have been used successfully for delivery of antigens where the immunogen is expressed on the spore surface. In this work the spore has been engineered to deliver antigens to the cytoplasm of macrophages by expressing listeriolysin O (LLO) or a derivative, L...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2008.09.024
更新日期：2008-11-11 00:00:00
abstract：:Chlamydia trachomatis infections can occur early in life and may result in long-term sequelae. To assess the feasibility of implementing a vaccine in newborns, groups of 2-day-old BALB/c mice were immunized intranasally (i.n.) with 1x10(4) inclusion forming units (IFU) of C. trachomatis mouse pneumonitis (MoPn). As a ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2005.06.026
更新日期：2005-11-16 00:00:00
abstract：:Nitric oxide (NO) mediated mechanisms have been implicated in killing of some life-stages of Brugia malayi/Wuchereria bancrofti and protect the host through type 1 responses and IFN-γ stimulated toxic mediators' release. However, the identity of NO stimulating molecules of the parasites is not known. Three predominant...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2014.11.041
更新日期：2015-01-15 00:00:00
abstract：:By using a baculovirus expression system, we have successfully produced simian immunodeficiency virus (SIV)-like particles (VLPs) with high levels of biologically active SIV envelope (Env) incorporated on their surfaces. To study whether SIV VLPs represent effective mucosal immunogens, we immunized groups of mice with...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(02)00160-3
更新日期：2002-06-07 00:00:00
abstract：:Japanese encephalitis (JE) vaccine has been used in childhood immunization programmes in Asia for many years. Also travellers from other parts of the world have been vaccinated before travelling in Asian countries. Recommendations for the use of JE vaccine are still debated because severe cases of adverse reactions ha...
journal_title：Vaccine
pub_type： 临床试验,杂志文章
doi：10.1016/s0264-410x(97)00020-0
更新日期：1997-08-01 00:00:00
abstract：BACKGROUND:To contribute to the global demand for oral cholera vaccine (OCV), the production of Euvichol® was scaled up with elimination of thimerosal. To demonstrate the equivalence of the variations, a study was carried out in the Philippines. METHODS:Healthy male and female adults and children in Manila were random...
journal_title：Vaccine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.vaccine.2018.05.102
更新日期：2018-07-05 00:00:00
abstract：:ErbB-2 is associated with several solid tumours of which breast cancer is the commonest cancer in women worldwide. Though anti-ErbB-2 antibody appears to play a significant role in prevention and therapy, naturally occurring anti-ErbB-2 antibody associated with the cleaved ectodomain of overexpressed ErbB-2 self antig...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2013.10.077
更新日期：2014-02-12 00:00:00
abstract：:Four different preparations of hepatitis B antigen (HBsAg) were tested in parallel with respect to their ability to elicit anti-HBs in guinea-pigs. We compared the effect of low doses (5 micrograms single dose and 30 micrograms total amount) of human plasma derived and recombinant HBsAg, in purified forms or prepared ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(90)90014-d
更新日期：1990-12-01 00:00:00
abstract：:For the production of a chemically inactivated Parapoxvirus ovis (PPVO), an adherent bovine kidney cell line was cultivated on Cytodex-3 microcarriers in suspension culture. The inactivated and purified virus particles have shown immune modulatory activity in several animal models. PPVO was produced by a biphasic batc...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2008.01.032
更新日期：2008-03-17 00:00:00
abstract：BACKGROUND:IXIARO® is a Vero cell-derived, inactivated Japanese encephalitis (JE) vaccine licensed mainly in western countries for children and adults traveling to JE endemic areas. Limited immunogenicity and safety data in elderly travelers have been available. OBJECTIVES:To evaluate safety and immunogenicity of IXIA...
journal_title：Vaccine
pub_type： 杂志文章,多中心研究
doi：10.1016/j.vaccine.2016.07.029
更新日期：2016-08-31 00:00:00
