Purification of O-specific polysaccharide from lipopolysaccharide produced by Salmonella enterica serovar Paratyphi A.

Abstract:

:The O specific polysaccharide (OSP) of the lipopolysaccharide (LPS) of Salmonella enterica serovar Paratyphi A is a protective antigen and the target for vaccine development. LPS is the major constituent of the outer membrane of S. Paratyphi A with the OSP exposed on the surface, in addition to the cell associated LPS a large amount of free LPS was present in the fermentation broth. A purification method was developed to take advantage of both sources of LPS and to maximize recovery of OSP. After fermentation the bacterial cells were concentrated and washed, the permeate containing the free LPS was processed separately from the cells. The free LPS was concentrated and washed on a 100kD ultrafiltration membrane to remove low molecular weight impurities. The LPS was then detoxified by separation of the lipid A from the OSP using acid hydrolysis at 100°C, the precipitated lipid A was removed by 0.2μm membrane filtration. Contaminants were then removed by acid precipitation in the presence of sodium deoxycholate. The OSP was concentrated and washed with 1M NaCl then water using a 10kD ultrafiltration membrane then sterile filtered through a 0.2μm membrane filter. The cells were treated by acid hydrolysis at 100°C, the remaining cells, cell debris and precipitate was removed by centrifugation. The filtrate was then treated in the same way as described above for the free LPS. The combined yield of purified OSP from free LPS plus the cells was greater than 880mg/L of culture broth. The method developed yields large amounts of OSP, is scalable and compatible with cGMP so would be readily transferrable to developing country vaccine manufacturers for low cost production of vaccine against S. Paratyphi A.

journal_name

Vaccine

journal_title

Vaccine

authors

Kothari S,Kim JA,Kothari N,Jones C,Choe WS,Carbis R

doi

10.1016/j.vaccine.2014.02.090

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

2457-62

issue

21

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(14)00317-X

journal_volume

32

pub_type

杂志文章

相关文献

VACCINE文献大全
  • Comparing vaccination hesitancy in Polish migrant parents who accept or refuse nasal flu vaccination for their children.

    abstract::This study investigates the background to low uptake of nasal influenza vaccination in Polish pupils in Edinburgh, Scotland. In autumn 2018, one week after their child's nasal flu vaccination sessions, 365 Polish parents were sent a questionnaire exploring influences on their vaccination choices. The questionnaire inc...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2020.02.028

    authors: Gorman DR,Bielecki K,Larson HJ,Willocks LJ,Craig J,Pollock KG

    更新日期:2020-03-17 00:00:00

  • Interventions to reduce the burden of vaccine-preventable diseases among migrants and refugees worldwide: A scoping review of published literature, 2006-2018.

    abstract:BACKGROUND:Disparities in vaccine-preventable disease (VPD) burden and immunisation coverage between migrants and refugees and their host populations have been described in numerous countries worldwide. Effective strategies are required to reduce the health disparities and immunisation inequities experienced by migrant...

    journal_title:Vaccine

    pub_type: 杂志文章,评审

    doi:10.1016/j.vaccine.2020.09.054

    authors: Charania NA,Gaze N,Kung JY,Brooks S

    更新日期:2020-10-27 00:00:00

  • Poly(propyleneimine) dendrimer and dendrosome mediated genetic immunization against hepatitis B.

    abstract::The purpose of the present research work is to explore the potential of dendrosomes in genetic immunization against hepatitis B. Plasmid DNA encoding pRc/CMV-HBs[S] (5.6 kb), encoding the small region of the hepatitis B surface antigen, was complexed with 5th generation poly(propyleneimine) dendrimer (PPI) in differen...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2008.04.058

    authors: Dutta T,Garg M,Jain NK

    更新日期:2008-06-25 00:00:00

  • Chapter 15: HPV vaccine use in the developing world.

    abstract::Human papillomavirus (HPV)-related morbidity and mortality from cervical cancer primarily occurs in the developing world, where, unfortunately, access to vaccines in general, and expensive newer vaccines in particular, is often more limited than in the industrialized world. In addition, secondary prevention methods su...

    journal_title:Vaccine

    pub_type: 杂志文章,评审

    doi:10.1016/j.vaccine.2006.05.128

    authors: Kane MA,Sherris J,Coursaget P,Aguado T,Cutts F

    更新日期:2006-08-31 00:00:00

  • The effect of pre-existing adenovirus-specific immunity on immune responses induced by recombinant adenovirus expressing glycoprotein D of bovine herpesvirus type 1.

    abstract::We investigated whether pre-existing adenovirus-specific immunity influenced the development of immunity to a foreign antigen expressed by recombinant adenovirus. Active adenovirus-specific immunity was induced in cotton rats by i.n. administration of wild type human adenovirus type 5 (HAd5) two weeks before immunisat...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/s0264-410x(98)00279-5

    authors: Papp Z,Babiuk LA,Baca-Estrada ME

    更新日期:1999-02-26 00:00:00

  • Immune response to bovine viral diarrhea virus (BVDV) vaccines detecting antibodies to BVDV subtypes 1a, 1b, 2a, and 2c.

    abstract::Bovine viral diarrhea virus (BVDV) represents a major cattle disease with multiple forms including fetal infections resulting in persistently infected (PI) cattle. The objectives of this study were to investigate the immune response to six vaccines, five modified live viral (MLV) and one killed vaccine containing BVDV...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2020.03.058

    authors: Fulton RW,Cook BJ,Payton ME,Burge LJ,Step DL

    更新日期:2020-05-19 00:00:00

  • A passive cold storage device economic model to evaluate selected immunization location scenarios.

    abstract:BACKGROUND:The challenge of keeping vaccines cold at health posts given the unreliability of power sources in many low- and middle-income countries and the expense and maintenance requirements of solar refrigerators has motivated the development of passive cold storage devices (PCDs), containers that keep vaccines cold...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2013.08.079

    authors: Norman BA,Nourollahi S,Chen SI,Brown ST,Claypool EG,Connor DL,Schmitz MM,Rajgopal J,Wateska AR,Lee BY

    更新日期:2013-10-25 00:00:00

  • Screening of pregnant women for hepatitis B.

    abstract::Neonatal infection with hepatitis B virus carries a very high risk of resulting in a persistent infection. Babies born to hepatitis B carrier mothers are at risk of infection through exposure to blood and body fluids during birth. These 'at risk' babies can only be identified through screening of all mothers during pr...

    journal_title:Vaccine

    pub_type: 杂志文章,评审

    doi:10.1016/s0264-410x(98)00289-8

    authors: Boxall E

    更新日期:1998-11-01 00:00:00

  • Interferon-gamma-inducing oral vaccination with Leishmania amazonensis antigens protects BALB/c and C57BL/6 mice against cutaneous leishmaniasis.

    abstract::The induction of oral tolerance against disease-inducing antigens has emerged as a feasible strategy to prevent immunopathologies. In this study, we investigated the effect of oral immunization with whole antigens of Leishmania amazonensis promastigotes (LaAg) on murine cutaneous leishmaniasis. We found that two oral ...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/s0264-410x(03)00427-4

    authors: Pinto EF,de Mello Cortezia M,Rossi-Bergmann B

    更新日期:2003-09-08 00:00:00

  • The changing landscape of respiratory syncytial virus.

    abstract::Recognition of the acute and chronic burden of respiratory syncytial virus (RSV) lower respiratory tract infections (LRTI) sparked a wave of initiatives to develop preventive and therapeutic products against the pathogen in recent years. RSV is a leading cause of hospitalization in infants in industrialized and develo...

    journal_title:Vaccine

    pub_type: 杂志文章,评审

    doi:10.1016/j.vaccine.2015.06.119

    authors: Polack FP

    更新日期:2015-11-25 00:00:00

  • HIV subtypes and recombination strains--strategies for induction of immune responses in man.

    abstract::Clinical and experimental studies of HIV-1 subcomponents were made in order to increase their immunogenicity. HIV subtype envelopes A, B and C have been compared and a detailed analysis made by peptides of the coreceptor-ligand interactions. We identified a direct interaction between HIV-1 envelope and a cellular rece...

    journal_title:Vaccine

    pub_type: 杂志文章,评审

    doi:10.1016/s0264-410x(02)00084-1

    authors: Wahren B,Ljungberg K,Rollman E,Levi M,Zuber B,Kjerrström Zuber A,Hinkula J,Leandersson AC,Calarota S,Hejdeman B,Bratt G,Sandström E

    更新日期:2002-05-06 00:00:00

  • Evaluation of a Salmonella Enteritidis vaccine and related ELISA for respective induction and assessment of acquired immunity to the vaccine and/or Echinacea purpurea in Awassi Ewes.

    abstract::The aim of this study was to evaluate an experimental Salmonella Enteritidis (SE) bacterin and an indirect ELISA system to assess quantitatively the acquired immunity in Awassi ewes to the vaccine and/or Echinacea purpurea (EP) dried roots. Four treatments of the ewes were included in the experimental design, with 6 e...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2015.03.035

    authors: Barbour EK,Assi CAA,Shaib H,Hamadeh S,Murtada M,Mahmoud G,Yaghmoor S,Iyer A,Harakeh S,Kumosani T

    更新日期:2015-05-05 00:00:00

  • Adjuvant Quil A improves protection in mice and enhances opsonic capacity of antisera induced by pneumococcal polysaccharide conjugate vaccines.

    abstract::The adjuvant effect of Quil A on the primary antibody response of mice to pneumococcal capsular polysaccharide conjugates was examined. Quil A increased the anti-capsular polysaccharide antibody titres, the protection against Streptococcus pneumoniae, and the opsonic capacity of the antibodies as measured in a newly d...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/0264-410x(94)90151-1

    authors: DeVelasco EA,Dekker HA,Antal P,Jalink KP,van Strijp JA,Verheul AF,Verhoef J,Snippe H

    更新日期:1994-11-01 00:00:00

  • Behavioral risk assessment in HIV Vaccine Trials Network (HVTN) clinical trials: a qualitative study exploring HVTN staff perspectives.

    abstract::In HIV vaccine trials, the collection and analysis of participant behavior data associated with risk of acquiring HIV-infection is important for a number of reasons. Although the rationale for behavioral risk assessment in HIV vaccine clinical trials is clear, consistent collection of behavioral data over time and acr...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2013.07.003

    authors: Andrasik MP,Karuna ST,Nebergall M,Koblin BA,Kublin JG,NIAID HIV Vaccine Trials Network.

    更新日期:2013-09-13 00:00:00

  • Double immunization strategy with a BoHV-4-vectorialized secreted chimeric peptide BVDV-E2/BoHV-1-gD.

    abstract::A bovine herpesvirus 4 was isolated from the milk cell fraction of a healthy cow and his full genome cloned as a bacterial artificial chromosome. So cloned viral genome was used as a vector platform to deliver in vitro and in vivo an optimized secreted chimeric peptide obtained by the fusion of the bovine viral diarrh...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2008.09.023

    authors: Donofrio G,Sartori C,Franceschi V,Capocefalo A,Cavirani S,Taddei S,Flammini CF

    更新日期:2008-11-11 00:00:00

  • Immunization of human hepatitis E viruses conferred protection against challenge by a camel hepatitis E virus.

    abstract::Dromedary camel hepatitis E virus is a novel HEV that belongs to the family Hepeviridae, and is classified as genotype 7 HEV (HEV-7). Since HEV-7 is transmitted from camels to humans and causes acute hepatitis E, this virus is a non-negligible pathogen for zoonosis, and a vaccine against HEV-7 infection is urgently ne...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2020.09.036

    authors: Guo Y,Yang F,Xu X,Feng M,Liao Y,He Z,Takeda N,Muramatsu M,Li Q,Li TC

    更新日期:2020-10-27 00:00:00

  • A meta-analysis of human papillomavirus type-distribution in women from South Asia: implications for vaccination.

    abstract:OBJECTIVE:To determine human papillomavirus (HPV) prevalence and type-distribution in women from South Asia, with and without cervical lesions, in order to estimate the impact of an HPV 16/18 prophylactic vaccine in this region and to assess additional types that should be incorporated in new vaccines. METHODS:A meta-...

    journal_title:Vaccine

    pub_type: 杂志文章,评审

    doi:10.1016/j.vaccine.2008.03.047

    authors: Bhatla N,Lal N,Bao YP,Ng T,Qiao YL

    更新日期:2008-06-02 00:00:00

  • Chapter 26: Innovative financing mechanisms to accelerate the introduction of HPV vaccines in developing countries.

    abstract::The costs of developing and producing new-generation vaccines have increased compared to many of the older, "traditional" vaccines because of new technologies and regulatory requirements. While the public sector often supports basic research costs, private manufacturers are usually responsible for the investments in p...

    journal_title:Vaccine

    pub_type: 杂志文章,评审

    doi:10.1016/j.vaccine.2006.05.042

    authors: Batson A,Meheus F,Brooke S

    更新日期:2006-08-31 00:00:00

  • Overview of rabies post-exposure prophylaxis access, procurement and distribution in selected countries in Asia and Africa, 2017-2018.

    abstract:BACKGROUND:Rabies is a neglected zoonotic disease with a global burden of approximately 59,000 human deaths a year. Once clinical symptoms appear, rabies is almost invariably fatal; however, with timely and appropriate post-exposure prophylaxis (PEP) consisting of wound washing, vaccine, and in some cases rabies immuno...

    journal_title:Vaccine

    pub_type: 杂志文章,评审

    doi:10.1016/j.vaccine.2019.04.024

    authors: Sreenivasan N,Li A,Shiferaw M,Tran CH,Wallace R,Blanton J,Knopf L,Abela-Ridder B,Hyde T,Working group on Rabies PEP logistics.

    更新日期:2019-10-03 00:00:00

  • Feasibility study of a combined diphtheria-tetanus-acellular pertussis-hepatitis B (DTPa-HBV) vaccine, and comparison of clinical reactions and immune responses with diphtheria-tetanus-acellular pertussis (DTPa) and hepatitis B vaccines applied as mixed o

    abstract::The feasibility of a combined diphtheria-tetanus-acellular pertussis-hepatitis B (DTPa-HBV) vaccine was assessed and a comparison made of immunogenicity and reactogenicity to DTPa and HBV vaccines mixed in one syringe and to concomitant but separate injections as a primary vaccination course in three groups of infants...

    journal_title:Vaccine

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1016/s0264-410x(97)00089-3

    authors: Usonis V,Bakasenas V,Willems P,Clemens R

    更新日期:1997-10-01 00:00:00

  • Progress towards hepatitis B prevention through vaccination in the Western Pacific, 1990-2014.

    abstract::Hepatitis B infections are responsible for more than 300 thousand deaths per year in the Western Pacific Region. Because of this high burden, the countries and areas of the Region established a goal of reducing hepatitis B chronic infection prevalence among children to less than 1% by 2017. This study was conducted to...

    journal_title:Vaccine

    pub_type: 杂志文章,评审

    doi:10.1016/j.vaccine.2016.03.060

    authors: Wiesen E,Diorditsa S,Li X

    更新日期:2016-05-27 00:00:00

  • A clinical trial to evaluate the safety and immunogenicity of the LEISH-F1+MPL-SE vaccine when used in combination with meglumine antimoniate for the treatment of cutaneous leishmaniasis.

    abstract::Forty-four adult patients with cutaneous leishmaniasis (CL) were enrolled in a randomized, double-blind, controlled, dose-escalating clinical trial and were randomly assigned to receive three injections of either the LEISH-F1+MPL-SE vaccine (consisting of 5, 10, or 20 μg recombinant Leishmania polyprotein LEISH-F1 ant...

    journal_title:Vaccine

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.vaccine.2010.07.063

    authors: Nascimento E,Fernandes DF,Vieira EP,Campos-Neto A,Ashman JA,Alves FP,Coler RN,Bogatzki LY,Kahn SJ,Beckmann AM,Pine SO,Cowgill KD,Reed SG,Piazza FM

    更新日期:2010-09-14 00:00:00

  • Experimental immunisation and protection of guinea pigs with Vibrio cholerae toxoid and mucinases, neuraminidase and proteinase.

    abstract::As measured by fluid accumulation in ileal loops, Vibrio cholerae mucinase complex, with or without toxoid, protected guinea pigs from challenges with V. cholerae live organisms and enterotoxin. The neuraminidase and proteinases of the complex were combined in modified oil emulsion or aluminum hydroxide adjuvants and ...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2003.12.004

    authors: Stewart-Tull DE,Lucas C,Bleakley CR

    更新日期:2004-06-02 00:00:00

  • The integration of barcode scanning technology into Canadian public health immunization settings.

    abstract:BACKGROUND:As part of a series of feasibility studies following the development of Canadian vaccine barcode standards, we compared barcode scanning with manual methods for entering vaccine data into electronic client immunization records in public health settings. METHODS:Two software vendors incorporated barcode scan...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2013.11.015

    authors: Pereira JA,Quach S,Hamid JS,Quan SD,Diniz AJ,Van Exan R,Malawski J,Finkelstein M,Samanani S,Kwong JC,Public Health Agency of Canada\/Canadian Institutes of Health Research Influenza Research Network (PCIRN) Program Delivery and Evalu

    更新日期:2014-05-13 00:00:00

  • Vaccinator device for delivering propellant-driven aerosols of Streptococcus suis bacterin into the respiratory tracts of swine.

    abstract::Metered-dose propellant-driven small particle aerosols of a killed whole bacterium, Streptococcus suis, were produced and characterized for their aerodynamic particle sizes and antigenicity as potential respiratory mucosal vaccines against S. suis infections in swine. To facilitate the efficient delivery of such vacci...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/s0264-410x(97)00297-1

    authors: Brown AR,George DW,Matteson DK

    更新日期:1997-08-01 00:00:00

  • Early studies on DNA-based immunizations for measles virus.

    abstract::DNA-mediated immunizations have been used to raise neutralizing antibodies for measles virus. Single inoculations of plasmids expressing measles hemagglutinin or fusion glycoproteins raised neutralizing antibody in BALB/c mice. Plasmids expressing the hemagglutinin glycoprotein (both normal and secreted) raised neutra...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/s0264-410x(96)00261-7

    authors: Yang K,Mustafa F,Valsamakis A,Santoro JC,Griffin DE,Robinson HL

    更新日期:1997-06-01 00:00:00

  • Maternal immune status influences HIV-specific immune responses in pups after DNA prime protein boost using mucosal adjuvant.

    abstract::This study was designed to determine the impact of maternal HIV-1 specific immunity on HIV-DNA immunization of 2-week-old pups during the breast-feeding period. Adult female mice received intranasal or intradermal HIV-DNA (gp160Env, p37Gag, Nef, Tat and Rev) prime and recombinant protein boost immunizations, which ind...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2008.08.060

    authors: Bråve A,Johansen K,Palma P,Benthin R,Hinkula J

    更新日期:2008-11-05 00:00:00

  • Breastfeeding linked to the reduction of both rotavirus shedding and IgA levels after Rotarix® immunization in Mexican infants.

    abstract::We examined potential risk factors on vaccine virus shedding and antibody seroresponse to human rotavirus vaccine (Rotarix) in Mexican infants. Two doses of Rotarix were administered to infants during the first two visits for their routine childhood immunization (∼8 and 15weeks of age) in Mexico City. Infant's charact...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2016.09.006

    authors: Bautista-Marquez A,Velasquez DE,Esparza-Aguilar M,Luna-Cruz M,Ruiz-Moran T,Sugata K,Jiang B,Parashar U,Patel M,Richardson V

    更新日期:2016-10-17 00:00:00

  • Effect of different adjuvant formulations on the immunogenicity and protective effect of a live Mycoplasma hyopneumoniae vaccine after intramuscular inoculation.

    abstract::Mycoplasma hyopneumoniae (M. hyopneumoniae) vaccine strain 168 is an intrapulmonically injected attenuated live vaccine that is available in the Chinese market. The aim of this study was to develop suitable adjuvants for this live vaccine to provide effective protection after intramuscular inoculation. Several adjuvan...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2014.03.071

    authors: Xiong Q,Wei Y,Xie H,Feng Z,Gan Y,Wang C,Liu M,Bai F,Xie F,Shao G

    更新日期:2014-06-05 00:00:00

  • A follow-up study of combined vaccination with plasma-derived and recombinant hepatitis B vaccines in infants.

    abstract::This study was aimed to evaluate the efficacy and immunogenicity of combined hepatitis B vaccination with plasma-derived vaccine (PDV) and recombinant vaccine (RV). A total of 329 infants was recruited, including 224 high-risk infants born to hepatitis B e antigen-positive mothers and 105 low-risk infants born to hepa...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/0264-410x(95)00108-d

    authors: Lee PI,Lee CY,Huang LM,Chen JM,Chang MH

    更新日期:1995-12-01 00:00:00