Purification of O-specific polysaccharide from lipopolysaccharide produced by Salmonella enterica serovar Paratyphi A.


:The O specific polysaccharide (OSP) of the lipopolysaccharide (LPS) of Salmonella enterica serovar Paratyphi A is a protective antigen and the target for vaccine development. LPS is the major constituent of the outer membrane of S. Paratyphi A with the OSP exposed on the surface, in addition to the cell associated LPS a large amount of free LPS was present in the fermentation broth. A purification method was developed to take advantage of both sources of LPS and to maximize recovery of OSP. After fermentation the bacterial cells were concentrated and washed, the permeate containing the free LPS was processed separately from the cells. The free LPS was concentrated and washed on a 100kD ultrafiltration membrane to remove low molecular weight impurities. The LPS was then detoxified by separation of the lipid A from the OSP using acid hydrolysis at 100°C, the precipitated lipid A was removed by 0.2μm membrane filtration. Contaminants were then removed by acid precipitation in the presence of sodium deoxycholate. The OSP was concentrated and washed with 1M NaCl then water using a 10kD ultrafiltration membrane then sterile filtered through a 0.2μm membrane filter. The cells were treated by acid hydrolysis at 100°C, the remaining cells, cell debris and precipitate was removed by centrifugation. The filtrate was then treated in the same way as described above for the free LPS. The combined yield of purified OSP from free LPS plus the cells was greater than 880mg/L of culture broth. The method developed yields large amounts of OSP, is scalable and compatible with cGMP so would be readily transferrable to developing country vaccine manufacturers for low cost production of vaccine against S. Paratyphi A.






Kothari S,Kim JA,Kothari N,Jones C,Choe WS,Carbis R




Has Abstract


2014-05-01 00:00:00
















  • Development of an attenuated strain of chikungunya virus for use in vaccine production.

    abstract::An attenuated chikungunya (CHIK) virus clone was developed for production of a live vaccine for human use. CHIK strain 15561 was subjected to 18 plaque-to-plaque passages in MRC-5 cultures before CHIK 181/clone 25 was selected as vaccine seed based on homogeneous small plaque size, suckling mouse avirulence, reduced m...


    pub_type: 杂志文章


    authors: Levitt NH,Ramsburg HH,Hasty SE,Repik PM,Cole FE Jr,Lupton HW

    更新日期:1986-09-01 00:00:00

  • Vaccines in the era of genomics: the pneumococcal challenge.

    abstract::In this review we aim to provide the reader with an understanding of the capsular-based complexity of Streptococcus pneumoniae, one of the main limitations to current vaccine development. We then discuss the need for a new vaccine strategy based on proteic antigen candidates discovered in silico. Describing specifical...


    pub_type: 杂志文章,评审


    authors: Barocchi MA,Censini S,Rappuoli R

    更新日期:2007-04-20 00:00:00

  • Enhancement of antigen presentation of influenza virus hemagglutinin by the natural human anti-Gal antibody.

    abstract::Immunogenicity of inactivated virus or subviral vaccines may be enhanced by complexing with an IgG antibody. Such antibody would increase the uptake, processing and presentation of the vaccine's antigens by antigen presenting cells (APC), via the adhesion of the antibody-vaccine complex to Fc-receptors on macrophages ...


    pub_type: 杂志文章


    authors: Galili U,Repik PM,Anaraki F,Mozdzanowska K,Washko G,Gerhard W

    更新日期:1996-03-01 00:00:00

  • Mucosal immunization with liposome-nucleic acid adjuvants generates effective humoral and cellular immunity.

    abstract::Development of effective new mucosal vaccine adjuvants has become a priority with the increase in emerging viral and bacterial pathogens. We previously reported that cationic liposomes complexed with non-coding plasmid DNA (CLDC) were effective parenteral vaccine adjuvants. However, little is known regarding the abili...


    pub_type: 杂志文章


    authors: Henderson A,Propst K,Kedl R,Dow S

    更新日期:2011-07-18 00:00:00

  • Human papillomavirus (HPV) vaccination and subsequent sexual behaviour: evidence from a large survey of Nordic women.

    abstract:OBJECTIVE:To assess whether recipients and non-recipients of the human papillomavirus (HPV) vaccine subsequently differ in terms of sexual risk taking behaviour. DESIGN:Cross-sectional survey. Sequential analyses constructed from self-reported age at vaccination, age at first intercourse and age at response. SETTING:...


    pub_type: 杂志文章


    authors: Hansen BT,Kjær SK,Arnheim-Dahlström L,Liaw KL,Jensen KE,Thomsen LT,Munk C,Nygård M

    更新日期:2014-09-03 00:00:00

  • Sero-epidemiology of measles and mumps in Korea: impact of the catch-up campaign on measles immunity.

    abstract::A catch-up campaign targeting children aged 8-16 years using measles-rubella (MR) vaccine was conducted during 2001 in Korea. To evaluate the impact of the campaign and assess mumps immunity, human IgG antibodies were detected using ELISA for measles (5826 samples) and mumps (5890 samples) in a national sample of oppo...


    pub_type: 杂志文章,多中心研究


    authors: Kim SS,Han HW,Go U,Chung HW

    更新日期:2004-12-02 00:00:00

  • Role of lipid structure in the humoral immune response in mice to covalent lipid-peptides from the membrane proximal region of HIV-1 gp41.

    abstract::The membrane proximal region (MPR) of HIV-1 gp41 is a desirable target for development of a vaccine that elicits neutralizing antibodies since the patient-derived monoclonal antibodies, 2F5 and 4E10, bind to the MPR and neutralize primary HIV isolates. The 2F5 and 4E10 antibodies cross-react with lipids and structural...


    pub_type: 杂志文章


    authors: Watson DS,Szoka FC Jr

    更新日期:2009-07-23 00:00:00

  • Internationally adopted children: what vaccines should they receive?

    abstract::It is of paramount importance to know the vaccination status in internationally adopted children, so that they can be correctly immunized. This study ascertains the seroprotection rate for vaccine-preventable diseases and the validity of the immunization cards in 637 adopted children. The absence of the immunization c...


    pub_type: 杂志文章


    authors: Cilleruelo MJ,de Ory F,Ruiz-Contreras J,González-González R,Mellado MJ,García-Hortelano M,Villota J,García-Ascaso M,Piñeiro R,Martín-Fontelos P,Herruzo R

    更新日期:2008-10-29 00:00:00

  • European citizens' opinions on immunisation.

    abstract::As part of a larger study exploring how European citizens' balance issues of public and private interest and the extent to which they are prepared to accept State intervention on a range of public health issues, focus group participants were asked whether childhood immunisation should be a matter of parental choice or...


    pub_type: 杂志文章


    authors: Moran N,Shickle D,Richardson E

    更新日期:2008-01-17 00:00:00

  • Report of the sixth AREB meeting, Manila, The Philippines, 10-12 November 2009.

    abstract::During their sixth annual meeting held in Manila (Philippines), the Asian Rabies Expert Bureau (AREB) reviewed the implementation of programs for rabies prevention, control, and elimination in Asia. AREB members strongly support a "one health" approach for controlling rabies, combining increased public awareness, comm...




    authors: Dodet B,Asian Rabies Expert Bureau (AREB).

    更新日期:2010-04-26 00:00:00

  • Long-term effectiveness of zoster vaccine live for postherpetic neuralgia prevention.

    abstract:BACKGROUND:Postherpetic neuralgia (PHN) occurs in 5-30% of individuals with herpes zoster (HZ) and is characterized by long-lasting pain. Zoster vaccine live (ZVL) is licensed for people 50 years and older to prevent HZ and PHN. This study evaluated vaccine effectiveness (VE) of ZVL against PHN. METHODS:We conducted a...


    pub_type: 杂志文章


    authors: Klein NP,Bartlett J,Fireman B,Marks MA,Hansen J,Lewis E,Aukes L,Saddier P

    更新日期:2019-08-23 00:00:00

  • Newborn universal immunisation against hepatitis B: immunogenicity and reactogenicity of simultaneous administration of diphtheria/tetanus/pertussis (DTP) and oral polio vaccines with hepatitis B vaccine at 0, 2 and 6 months of age.

    abstract::The reactogenicity and immunogenicity of the administration of recombinant vaccine against hepatitis B simultaneously (but at separate sites) with diphtheria/tetanus/pertussis (DTP) and oral polio vaccines were examined. Six hundred and twenty-six children (group I) were given hepatitis B vaccine at 0, 2 and 6 months ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Aristegui J,Muñiz J,Pérez Legorburu A,Imaz M,Arrate JP,Suárez MD,Goiri MD

    更新日期:1995-08-01 00:00:00

  • A comparative study of PENTA vaccine booster doses given at 12, 15, or 18 months of age.

    abstract::For infants immunized with Haemophilus influenzae type b conjugate vaccines, booster immunization is usually recommended in the second year of life, typically between 12 and 18 months. This study assessed the effect of age at booster immunization on pre-immunization antibody trough levels and on subsequent responses, ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Scheifele DW,Guasparini R,Lavigne P

    更新日期:1999-02-12 00:00:00

  • The Global Meningococcal Initiative: recommendations for reducing the global burden of meningococcal disease.

    abstract::The Global Meningococcal Initiative (GMI) is composed of an international group of scientists, clinicians and public health officials with expertise in meningococcal immunology, epidemiology and prevention. The primary goal of the GMI is the promotion of the global prevention of invasive meningococcal disease through ...


    pub_type: 杂志文章


    authors: Harrison LH,Pelton SI,Wilder-Smith A,Holst J,Safadi MA,Vazquez JA,Taha MK,LaForce FM,von Gottberg A,Borrow R,Plotkin SA

    更新日期:2011-04-18 00:00:00

  • Development of a baited oral vaccine for use in reservoir-targeted strategies against Lyme disease.

    abstract::Lyme disease is a major human health problem which continues to increase in incidence and geographic distribution. As a vector-borne zoonotic disease, Lyme disease may be amenable to reservoir targeted strategies for control. We have previously reported that a vaccinia virus (VV) based vaccine expressing outer surface...


    pub_type: 杂志文章


    authors: Bhattacharya D,Bensaci M,Luker KE,Luker G,Wisdom S,Telford SR,Hu LT

    更新日期:2011-10-13 00:00:00

  • Efficacies of whole cell and acellular pertussis vaccines against Bordetella parapertussis in a mouse model.

    abstract::Pertussis vaccine development has mainly focused on Bordetella pertussis, and consequently these vaccines contain B. pertussis antigens only. However, the related species Bordetella parapertussis can also cause pertussis, although symptoms associated with the disease are generally considered to be milder. Recent field...


    pub_type: 杂志文章


    authors: David S,van Furth R,Mooi FR

    更新日期:2004-05-07 00:00:00

  • Protective immunity against tick infestation in cattle vaccinated with recombinant trypsin inhibitor of Rhipicephalus microplus.

    abstract::The cattle tick, Rhipicephalus microplus, is regarded as the most economically important ectoparasite of livestock globally. Control is achieved primarily through the use of acaricides. This approach is hampered by the development of resistance to commercial acaricides among cattle tick populations. Vaccination agains...


    pub_type: 杂志文章


    authors: Andreotti R,Cunha RC,Soares MA,Guerrero FD,Leite FP,de León AA

    更新日期:2012-10-19 00:00:00

  • Duration of Vi antibodies in participants vaccinated with Typhim Vi (Typhoid Vi polysaccharide vaccine) in an area not endemic for typhoid fever.

    abstract::After a single injection of Typhim Vi (typhoid Vi polysaccharide vaccine), serum antibody concentrations were monitored for 3 years in 37 adults who resided where typhoid fever was not endemic. Anti-Vi antibody concentrations declined progressively during the study, to levels that support the current US recommendation...


    pub_type: 杂志文章


    authors: Froeschle JE,Decker MD

    更新日期:2010-02-10 00:00:00

  • Humoral and cellular immunity induced by antigens adjuvanted with colloidal iron hydroxide.

    abstract::The immunopotentiating activities of colloidal iron hydroxide, a novel, experimental mineral adjuvant, and of aluminium hydroxide. the licensed adjuvant for human vaccines, were compared. Our studies revealed that colloidal iron hydroxide and aluminium hydroxide behaved comparably with respect to supporting induction ...


    pub_type: 杂志文章


    authors: Leibl H,Tomasits R,Brühl P,Kerschbaum A,Eibl MM,Mannhalter JW

    更新日期:1999-03-05 00:00:00

  • Adaptive acid tolerance response by Salmonella typhi and candidate live oral typhoid vaccine strains.

    abstract::The data presented here demonstrate that Salmonella typhi is capable of expressing an acid tolerance response (ATR) and that effective induction of this response (in nutrient-rich medium) occurs at pH 5.0 in anaerobic conditions. The candidate live oral S. typhi vaccine strains made by precise genetic methods and whic...


    pub_type: 杂志文章


    authors: Hone DM,Harris AM,Levine MM

    更新日期:1994-08-01 00:00:00

  • Pertussis antitoxin decay after vaccination with DTPa. Response to a first booster dose 3 1/2-6 1/2 years after the third vaccine dose.

    abstract::Longitudinal serum samples were collected from 542 children that had participated in a Swedish pertussis vaccine trial 1992-1995 [Gustafsson L, Hallander HO, Olin P, Reizenstein E, Storsaeter J. A controlled trial of a two-component acellular, a five-component acellular, and a whole-cell pertussis vaccine. N Engl J Me...


    pub_type: 杂志文章,随机对照试验


    authors: Hallander HO,Gustafsson L,Ljungman M,Storsaeter J

    更新日期:2005-11-16 00:00:00

  • Proteomic analysis of influenza haemagglutinin-specific antibodies following vaccination reveals convergent immunoglobulin variable region signatures.

    abstract::Analysis of the anti-haemagglutinin serum antibody proteome from six H1N1pdm09 influenza A vaccinated subjects demonstrated restricted IgG1 heavy chain species encoded by IGHV5-51 and IGHV3-7 gene families in 2 subjects and either IGHV5-51 or IGHV3-7 in 4 individuals. All subjects exhibited a dominant IGKV3-20 light c...


    pub_type: 杂志文章


    authors: Adamson PJ,Al Kindi MA,Wang JJ,Colella AD,Chataway TK,Petrovsky N,Gordon TP,Gordon DL

    更新日期:2017-10-09 00:00:00

  • Anti-HBs levels after hepatitis B immunisation depend on test reagents: routinely determined 10 and 100 IU/l seroprotection levels unreliable.

    abstract::In a large series of post-vaccination samples we compared the result of three commercially available anti-HBs assays (AxSYM, Architect and Access) on the quantitation of anti-HBs after immunisation with Engerix-B (HBsAg/ad) and GenHevacB (HBsAg/ay) vaccine. Two of the assays (AxSYM, Architect: Abbott Laboratories) gav...


    pub_type: 杂志文章


    authors: Heijtink RA,Schneeberger PM,Postma B,Crombach W

    更新日期:2002-07-26 00:00:00

  • Progress towards a vaccine against Ebola to meet emergency medical countermeasure needs.

    abstract::The Ebola virus epidemic in West Africa proved to be the largest in the history of filovirus outbreaks, causing the World Health Organization to declare a public health emergency of international concern in August of 2014. In collaboration with domestic and international partners, the Biomedical Advanced Research and ...


    pub_type: 杂志文章


    authors: Wolfe DN,Zarrabian AG,Disbrow GL,Espeland EM

    更新日期:2019-11-15 00:00:00

  • Familial clustering of hepatitis B virus infections and prevention of perinatal transmission by immunization with a reduced number of doses in an area of intermediate endemicity (Tunisia).

    abstract::Hepatitis B surface antigen (HBsAg) was detected in 3.3% of 7162 pregnant Tunisian women tested and HBeAg in 9.6% of the HBsAg-positive mothers. Family members of 46 of these HBsAg-positive mothers (33 husbands and 61 children aged 1-6 years) were investigated for the presence of HBV markers. HBsAg was detected in 21%...


    pub_type: 杂志文章


    authors: Coursaget P,Gharbi Y,Khrouf N,Depril N,Boukhris N,Fritzell B,Kastally R

    更新日期:1994-01-01 00:00:00

  • Global distribution of group A rotavirus strains in horses: a systematic review.

    abstract::Group A rotavirus (RVA) is a major cause of diarrhea and diarrhea-related mortality in foals in parts of the world. In addition to careful horse farm management, vaccination is the only known alternative to reduce the RVA associated disease burden on horse farms. The precise evaluation of vaccine effectiveness against...


    pub_type: 杂志文章,评审


    authors: Papp H,Matthijnssens J,Martella V,Ciarlet M,Bányai K

    更新日期:2013-11-19 00:00:00

  • Acceptability of an inactivated influenza vaccine delivered by microneedle patch: Results from a phase I clinical trial of safety, reactogenicity, and immunogenicity.

    abstract:OBJECTIVE:This study sought to evaluate the acceptability of inactivated influenza vaccine delivered by microneedle patch (MNP) in comparison to inactivated influenza vaccine (IIV) delivered by hypodermic needle. DESIGN, SETTING, AND PARTICIPANTS:From the general population of Atlanta, Georgia, we screened 112 and enr...


    pub_type: 杂志文章


    authors: Frew PM,Paine MB,Rouphael N,Schamel J,Chung Y,Mulligan MJ,Prausnitz MR

    更新日期:2020-10-21 00:00:00

  • N-fragment of edema factor as a candidate antigen for immunization against anthrax.

    abstract::The nontoxic N-terminal fragment of Bacillus anthracis edema factor (EF) was evaluated as a candidate antigen in an anthrax vaccine using a replication-incompetent adenoviral vector. An E1/E3 deleted adenovirus (Ad/EFn) encoding the N-terminal region 1-254 amino acids of the edema factor (EFn) was constructed using th...


    pub_type: 杂志文章


    authors: Zeng M,Xu Q,Hesek ED,Pichichero ME

    更新日期:2006-01-30 00:00:00

  • An enriched medical home intervention using community health workers improves adherence to immunization schedules.

    abstract:IMPORTANCE:Disparities in childhood vaccination rates persist. OBJECTIVE:To evaluate the impact of an enriched medical home intervention using community health workers on improving immunization adherence among young children. DESIGN:The intervention group received home visits from trained community health workers to ...


    pub_type: 杂志文章


    authors: Pati S,Ladowski KL,Wong AT,Huang J,Yang J

    更新日期:2015-11-17 00:00:00

  • Protective and disease-enhancing immune responses induced by recombinant modified vaccinia Ankara (MVA) expressing respiratory syncytial virus proteins.

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    pub_type: 杂志文章


    authors: Olszewska W,Suezer Y,Sutter G,Openshaw PJ

    更新日期:2004-11-25 00:00:00