Fine-mapping natural alleles: quantitative complementation to the rescue.


:Mapping the genes responsible for natural variation and divergence is a challenging task. Many studies have mapped genes to genomic regions or generated lists of candidates, but few studies have implicated specific genes with a high standard of evidence. I propose that combining recent advances in genomic engineering with a modified version of the quantitative complementation test will help turn candidate genes into causal genes. By creating loss-of-function mutations in natural strains, and using these mutations to quantitatively fail-to-complement natural alleles, fine mapping should be greatly facilitated. As an example, I propose that the CRISPR/Cas9 system could be combined with the FLP/FRT system to fine-map genes in the numerous systems where inversions have frustrated these efforts.


Mol Ecol


Molecular ecology


Turner TL




Has Abstract


2014-05-01 00:00:00












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    authors: Blanco-Pastor JL,Fernández-Mazuecos M,Vargas P

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    pub_type: 杂志文章


    authors: Gamache I,Jaramillo-Correa JP,Payette S,Bousquet J

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    pub_type: 杂志文章


    authors: Baltazar-Soares M,Blanchet S,Cote J,Tarkan AS,Záhorská E,Gozlan RE,Eizaguirre C

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    pub_type: 评论,杂志文章


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    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Arioli M,Jakob C,Reyer HU

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    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Mosca E,Eckert AJ,Di Pierro EA,Rocchini D,La Porta N,Belletti P,Neale DB

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