Abstract:
:Mapping the genes responsible for natural variation and divergence is a challenging task. Many studies have mapped genes to genomic regions or generated lists of candidates, but few studies have implicated specific genes with a high standard of evidence. I propose that combining recent advances in genomic engineering with a modified version of the quantitative complementation test will help turn candidate genes into causal genes. By creating loss-of-function mutations in natural strains, and using these mutations to quantitatively fail-to-complement natural alleles, fine mapping should be greatly facilitated. As an example, I propose that the CRISPR/Cas9 system could be combined with the FLP/FRT system to fine-map genes in the numerous systems where inversions have frustrated these efforts.
journal_name
Mol Ecoljournal_title
Molecular ecologyauthors
Turner TLdoi
10.1111/mec.12719subject
Has Abstractpub_date
2014-05-01 00:00:00pages
2377-82issue
10eissn
0962-1083issn
1365-294Xjournal_volume
23pub_type
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