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Isolation and characterization of monoclonal antibodies against ribonuclease inhibitor.

Abstract：

:Mouse monoclonal antibodies were generated against human ribonuclease inhibitor, an intracellular regulatory protein. A total of four antibodies were isolated, all of which were of the immunoglobulin G1 subtype. Western blot analysis of the antibodies suggested monospecificity. Based on immunoradiometric competition assays two of the antibodies were determined to be directed against the same antigenic epitope, while the other two were against a second and possibly third epitope. None of the antibodies appeared to be directed against the ribonuclease binding site of the antigen. Data is presented suggesting that ribonuclease inhibitor is present in normal human serum. The potential significance of these findings is discussed.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Feldman M,Kohtz DS,Kleinberg DL

doi

10.1016/s0006-291x(88)80045-7

subject

Has Abstract

pub_date

1988-11-30 00:00:00

pages

286-94

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(88)80045-7

journal_volume

157

pub_type

杂志文章

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