GOLPH3 is a novel marker of poor prognosis and a potential therapeutic target in human renal cell carcinoma.


BACKGROUND:Golgi phosphoprotein 3 (GOLPH3) has been reported to be involved in the development of several human cancers. The present study was conducted to investigate the expression of GOLPH3 and its prognostic significance in renal cell carcinoma (RCC). Meanwhile, the function of GOLPH3 in human RCC was further investigated in cell culture models. METHODS:Expression of GOLPH3 was examined in 43 fresh RCC tissues and paired adjacent normal renal tissues by real-time quantitative PCR and western blotting. Immunohistochemistry for GOLPH3 was performed on additional 218 RCC tissues. The clinical significance of GOLPH3 expression was analysed. Downregulation of GOLPH3 was performed using small-interfering RNA (siRNA) in Caki-1 and 786-O cells with high abundance of GOLPH3, and the effects of GOLPH3 silencing on cell proliferation, migration, invasion in vitro, and tumour growth in vivo were evaluated. RESULTS:Expression of GOLPH3 was upregulated in the majority of the RCC clinical tissue specimens at both mRNA and protein levels. Clinicopathological analysis showed that GOLPH3 expression was significantly correlated with T stage (P<0.001), lymph-node status (P=0.003), distant metastasis (P<0.001), tumour-node-metastasis (TNM) stage (P<0.001), and Fuhman grade (P=0.001). Expression of GOLPH3 was inversely correlated with both overall and recurrence-free survival of RCC patients. Multivariate analysis showed that GOLPH3 expression was an independent prognostic indicator for patient's survival. Knockdown of the GOLPH3 expression reduced cell proliferation, anchorage-independent growth, migration, invasion, and tumour growth in xenograft model mice. CONCLUSIONS:These results suggest that GOLPH3 expression is likely to have important roles in RCC development and progression, and that GOLPH3 is a prognostic biomarker and a promising therapeutic target for RCC.


Br J Cancer


Xue Y,Wu G,Liao Y,Xiao G,Ma X,Zou X,Zhang G,Xiao R,Wang X,Liu Q,Long D,Yang J,Xu H,Liu F,Liu M,Xie K,Huang R




Has Abstract


2014-04-29 00:00:00














  • Prognostic and predictive value of plasma testosterone levels in patients receiving first-line chemotherapy for metastatic castrate-resistant prostate cancer.

    abstract:BACKGROUND:Biomarkers for metastatic castration-resistant prostatic cancer (mCRPC) are an unmet medical need. METHODS:The prognostic and predictive value for survival and response to salvage hormonal therapy (SHT) of baseline testosterone level (TL) was analysed in a cohort of 101 mCRPC patients participating in 9 non...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: de Liaño AG,Reig O,Mellado B,Martin C,Rull EU,Maroto JP

    更新日期:2014-04-29 00:00:00

  • Vascular aging in long-term survivors of testicular cancer more than 20 years after treatment with cisplatin-based chemotherapy.

    abstract:BACKGROUND:Late effects of cisplatin-based chemotherapy in testicular cancer survivors (TCS) include cardiovascular morbidity, but little data is available beyond 20 years. The objective was to assess vascular damage in very long-term TCS. METHODS:TCS (treated with chemotherapy or orchiectomy only) and age-matched hea...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Stelwagen J,Lubberts S,Steggink LC,Steursma G,Kruyt LM,Donkerbroek JW,van Roon AM,van Gessel AI,van de Zande SC,Meijer C,Gräfin Zu Eulenburg CH,Oosting SF,Nuver J,Walenkamp AME,Jan de Jong I,Lefrandt JD,Gietema JA

    更新日期:2020-11-01 00:00:00

  • Cancer of the testis and month of birth.

    abstract::It has recently been reported that a series of testis cancers shows a temporal cycle in birth dates with a peak in certain months. This observation has been tested by an examination of a larger series covering all testis cancers diagnosed in the years 1971-84 in England and Wales, in men born in 1940 or later. Limited...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Kinlen LJ,Willows AN

    更新日期:1987-05-01 00:00:00

  • One-step nucleic acid amplification assay is an accurate technique for sentinel lymph node biopsy of breast cancer patients: a meta-analysis.

    abstract:BACKGROUND:To estimate the accuracy of one-step nucleic acid amplification (OSNA) assay as an intra-operative sentinel lymph node biopsy (SLNB) for sentinel lymph node (SLN) metastasis in breast cancer. METHODS:PubMed, Cochrane Library and Web of Science databases were searched by two independent reviewers to retrieve...

    journal_title:British journal of cancer

    pub_type: 杂志文章,meta分析


    authors: Shi F,Zhang Q,Liang Z,Zhang M,Liu X

    更新日期:2017-10-10 00:00:00

  • Identification of two contiguous minimally deleted regions on chromosome 1p36.31-p36.32 in oligodendroglial tumours.

    abstract::Loss of the short arm of chromosome 1 is a hallmark of oligodendroglial tumours (OTs). Deletion mapping studies in OTs have revealed multiple commonly deleted regions on chromosome 1p, suggesting that there are more than one tumour suppressor gene. To map critical deletion regions on 1p, a series of 25 OTs were examin...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Dong Z,Pang JS,Ng MH,Poon WS,Zhou L,Ng HK

    更新日期:2004-09-13 00:00:00

  • Preliminary results of the use of intraperitoneal carbon-adsorbed mitomycin C in intra-abdominal malignancy.

    abstract::Eleven patients suffering from intra-abdominal malignancy were treated with various doses of intraperitoneal mitomycin C adsorbed onto activated carbon particles. Seven of the patients underwent resection of their primary gastric tumour and all developed potentially life-threatening severe complications that proved to...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Ubhi SS,McCulloch P,Veitch PS

    更新日期:1997-01-01 00:00:00

  • The effect of 1,25-dihydroxyvitamin D3 on lymphoma cell lines and expression of vitamin D receptor in lymphoma.

    abstract::1,25(OH)2D3 promotes differentiation and has an antiproliferative effect in a variety of cell lines derived from the immunohaematopoetic system. alpha-Calcidol which is metabolised to 1,25(OH)2D3 has been shown to produce tumour regression in follicular low grade non-Hodgkin's lymphoma (NHL) and the dose limiting toxi...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Hickish T,Cunningham D,Colston K,Millar BC,Sandle J,Mackay AG,Soukop M,Sloane J

    更新日期:1993-10-01 00:00:00

  • c-myc oncogene product expression and prognosis in operable breast cancer.

    abstract::The 62 kDa protein product of the c-myc oncogene (p62 c-myc) is thought to be involved in the control of normal cellular proliferation and differentiation. We have measured oncoprotein levels using a flow cytometric assay in 141 operable breast cancers and have correlated levels with prognostic variables, patient surv...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Locker AP,Dowle CS,Ellis IO,Elston CW,Blamey RW,Sikora K,Evan G,Robins RA

    更新日期:1989-11-01 00:00:00

  • Effect of misonidazole and hyperthermia on the radiosensitivity of a C3H mouse mammary carcinoma and its surrounding normal tissue.

    abstract::Both misonidazole (MISO) and hyperthermia are known to enhance the radiation response of hypoxic cells, and to be selectively cytotoxic against cells in a hypoxic and acidic environment. The ability of these conditions to modify the effect of irradiation and their individual relationship was studied in a C3H mammary c...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Overgaard J

    更新日期:1980-01-01 00:00:00

  • Role of MC1R variants in uveal melanoma.

    abstract::Variants of the melanocortin-1 receptor (MC1R) gene have been linked to sun-sensitive skin types and hair colour, and may independently play a role in susceptibility to cutaneous melanoma. To assess the role of MC1R variants in uveal melanoma, we have analysed a cohort of 350 patients for the changes within the major ...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Hearle N,Humphreys J,Damato BE,Wort R,Talaban R,Wixey J,Green H,Easton DF,Houlston RS

    更新日期:2003-11-17 00:00:00

  • Impact of mental health problems on case fatality in male cancer patients.

    abstract:BACKGROUND:Although mortality rates are elevated in psychiatric patients relative to their healthy counterparts, little is known about the impact of mental health on survival in people with cancer. METHODS AND RESULTS:Among 16 498 Swedish men with cancer, survival was worse in those with a history of psychiatric hospi...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Batty GD,Whitley E,Gale CR,Osborn D,Tynelius P,Rasmussen F

    更新日期:2012-05-22 00:00:00

  • Immunotherapy for acute myelogenous leukaemia.

    abstract::One hundred and seven untreated patients with acute myelogenous leukaemia (AML) were admitted to St Bartholomew's Hospital between 10 October 1970 and 31 January 1973. Before receiving drugs to induce remission they were allocated alternatively into 2 groups to decide their remission treatment-a group to receive chemo...

    journal_title:British journal of cancer

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Powles RL,Crowther D,Bateman CJ,Beard ME,McElwain TJ,Russell J,Lister TA,Whitehouse JM,Wrigley PF,Pike M,Alexander P,Fairley GH

    更新日期:1973-11-01 00:00:00

  • HER2 gene copy number status may influence clinical efficacy to anti-EGFR monoclonal antibodies in metastatic colorectal cancer patients.

    abstract:BACKGROUND:In metastatic colorectal cancer (mCRC), KRAS is the only validated biomarker used to select patients for administration of epidermal growth factor receptor (EGFR)-targeted therapies. To identify additional predictive markers, we investigated the importance of HER2, the primary EGFR dimerisation partner, in t...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Martin V,Landi L,Molinari F,Fountzilas G,Geva R,Riva A,Saletti P,De Dosso S,Spitale A,Tejpar S,Kalogeras KT,Mazzucchelli L,Frattini M,Cappuzzo F

    更新日期:2013-02-19 00:00:00

  • Cancer mortality by educational level in the city of Barcelona.

    abstract::The objective of this study was to examine the relationship between educational level and mortality from cancer in the city of Barcelona. The data were derived from a record linkage between the Barcelona Mortality Registry and the Municipal Census. The relative risks (RR) of death and 95% confidence intervals (CIs) ac...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Fernandez E,Borrell C

    更新日期:1999-02-01 00:00:00

  • No association between P53 codon 72 polymorphism and risk of squamous cell carcinoma of the head and neck.

    abstract::An initial report suggested that patients homozygous for the arginine allele at codon 72 of P53 were at increased risk for human papillomavirus (HPV)-related cervical cancer, but other groups have not confirmed this finding. Since approximately 18-36% of head and neck cancers are HPV-related, we examined the genotypic...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Hamel N,Black MJ,Ghadirian P,Foulkes WD

    更新日期:2000-02-01 00:00:00

  • Dacarbazine and interferon alpha with or without interleukin 2 in metastatic melanoma: a randomized phase III multicentre trial of the Dermatologic Cooperative Oncology Group (DeCOG).

    abstract::In several phase II-trials encouraging tumour responses rates in advanced metastatic melanoma (stage IV; AJCC-classification) have been reported for the application of biochemotherapy containing interleukin 2. This study was designed to compare the efficacy of therapy with dacarbazine (DTIC) and interferon alpha (IFN-...

    journal_title:British journal of cancer

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Hauschild A,Garbe C,Stolz W,Ellwanger U,Seiter S,Dummer R,Ugurel S,Sebastian G,Nashan D,Linse R,Achtelik W,Mohr P,Kaufmann R,Fey M,Ulrich J,Tilgen W

    更新日期:2001-04-20 00:00:00

  • Anti-tumour cytotoxin produced by human monocytes: studies on its mode of action.

    abstract::Human monocytes can be induced to synthesize a cytotoxin which affects certain tumour cell lines. The interaction of monocyte cytotoxin with a susceptible cell line (L929) has been studied to obtain clues to the mode of action of the cytotoxin. The cytotoxin acts directly on the cells rather than on the culture medium...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Matthews N

    更新日期:1983-09-01 00:00:00

  • Growth of human tumour cell colonies from biopsies using two soft-agar techniques.

    abstract::Two techniques for growing colonies of human tumour cells in soft agar have been applied to cell suspensions derived from fresh tumour tissue from 48 patients. Colonies were obtained in 31 cases, with plating efficiencies between 0.01 and 15%. In 11 cases the plating efficiencies were 1% or above. There was evidence t...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Courtenay VD,Selby PJ,Smith IE,Mills J,Peckham MJ

    更新日期:1978-07-01 00:00:00

  • Bcl-2 protein expression: association with p53 and prognosis in colorectal cancer.

    abstract::Bcl-2 expression in colorectal carcinomas was studied in a series of 224 patients and the relation to p53 expression, stage and survival assessed. Bcl-2 expression was down-regulated compared with normal mucosa in 67% (151) of the cases. In 144 cases staining was positive for p53 (MAB DO7), and 41 of these 144 p53-pos...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Kaklamanis L,Savage A,Whitehouse R,Doussis-Anagnostopoulou I,Biddolph S,Tsiotos P,Mortensen N,Gatter KC,Harris AL

    更新日期:1998-06-01 00:00:00

  • Prediction of ovarian cancer prognosis and response to chemotherapy by a serum-based multiparametric biomarker panel.

    abstract::Currently, there are no effective biomarkers for ovarian cancer prognosis or prediction of therapeutic response. The objective of this study was to examine a panel of 10 serum biochemical parameters for their ability to predict response to chemotherapy, progression and survival of ovarian cancer patients. Sera from ov...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Oikonomopoulou K,Li L,Zheng Y,Simon I,Wolfert RL,Valik D,Nekulova M,Simickova M,Frgala T,Diamandis EP

    更新日期:2008-10-07 00:00:00

  • Mutant, wild type, or overall p53 expression: freedom from clinical progression in tumours of astrocytic lineage.

    abstract::Abnormalities of the p53 tumor-suppressor gene are found in a significant proportion of astrocytic brain tumours. We studied tumour specimens from 74 patients evaluated over 20 years at the Massachusetts General Hospital, where clinical outcome could be determined and sufficient pathologic material was available for i...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Pardo FS,Hsu DW,Zeheb R,Efird JT,Okunieff PG,Malkin DM

    更新日期:2004-11-01 00:00:00

  • Indole-3-carbinol synergistically sensitises ovarian cancer cells to bortezomib treatment.

    abstract:BACKGROUND:Bortezomib is a proteasome inhibitor with minimal clinical activity as a monotherapy in solid tumours, but its combination with other targeted therapies is being actively investigated as a way to increase its anticarcinogenic properties. Here, we evaluate the therapeutic potential of co-treatment with bortez...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Taylor-Harding B,Agadjanian H,Nassanian H,Kwon S,Guo X,Miller C,Karlan BY,Orsulic S,Walsh CS

    更新日期:2012-01-17 00:00:00

  • Urinary N1, N12-diacetylspermine is a non-invasive marker for the diagnosis and prognosis of non-small-cell lung cancer.

    abstract:BACKGROUND:Early detection of non-small-cell lung cancer (NSCLC) and accurate prognostic risk assessment could improve patient outcome. We examined the significance of urinary N(1), N(12)-diacetylspermine (DiAcSpm) in the detection and prognostic stratification of NSCLC patients. METHODS:A DiAcSpm/cutoff ratio (DASr) ...

    journal_title:British journal of cancer

    pub_type: 杂志文章,多中心研究


    authors: Takahashi Y,Sakaguchi K,Horio H,Hiramatsu K,Moriya S,Takahashi K,Kawakita M

    更新日期:2015-11-17 00:00:00

  • The influence of tumour cell DNA content on survival in colorectal cancer: a detailed analysis.

    abstract::We have investigated the influence of tumour cell DNA content (ploidy) on survival of 416 patients undergoing excisional surgery for colorectal cancer. Two hundred and eleven (51%) tumours had an abnormal DNA content (aneuploid or tetraploid). There was no correlation between ploidy status, sex, age and pathological s...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Armitage NC,Ballantyne KC,Evans DF,Clarke P,Sheffield J,Hardcastle JD

    更新日期:1990-11-01 00:00:00

  • Intra-tumoural variation of oestrogen receptor status in endometrial cancer.

    abstract::Soluble and nuclear oestrogen receptor status was determined in both the central and peripheral portions of tumour for 37 cases of adenocarcinoma of the endometrium. Of these, 29 had functional receptor in the peripheral biopsy, but only 19 retained functional receptor in the centre. Six of the 10 patients whose tumou...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Castagnetta L,Lo Casto M,Mercadante T,Polito L,Cowan S,Leake RE

    更新日期:1983-02-01 00:00:00

  • Platelets enhance malignant behaviours of gastric cancer cells via direct contacts.

    abstract::In this study, we aimed to analyse human cancer cell-platelet interactions in functional cell analyses and explore the molecular mechanisms behind tumour progression. Various functional analyses of gastric cancer (GC) cells were performed after direct/indirect co-incubation with platelets derived from GC patients. Fur...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Saito R,Shoda K,Maruyama S,Yamamoto A,Takiguchi K,Furuya S,Hosomura N,Akaike H,Kawaguchi Y,Amemiya H,Kawaida H,Sudo M,Inoue S,Kono H,Suzuki-Inoue K,Ichikawa D

    更新日期:2020-10-28 00:00:00

  • Evaluation of Angiopoietin-2 as a biomarker in gastric cancer: results from the randomised phase III AVAGAST trial.

    abstract:BACKGROUND:In the phase III AVAGAST trial, the addition of bevacizumab to chemotherapy improved progression-free survival (PFS) but not overall survival (OS) in patients with advanced gastric cancer. We studied the role of Angiopoietin-2 (Ang-2), a key driver of tumour angiogenesis, metastasis and resistance to antiang...

    journal_title:British journal of cancer

    pub_type: 杂志文章,随机对照试验


    authors: Hacker UT,Escalona-Espinosa L,Consalvo N,Goede V,Schiffmann L,Scherer SJ,Hedge P,Van Cutsem E,Coutelle O,Büning H

    更新日期:2016-04-12 00:00:00

  • Longstanding survival without cancer progression in a patient affected by endometrial carcinoma treated primarily with leuprolide.

    abstract::We report here a case of a patient affected by endometrial cancer and treated primarily with leuprolide, the surgical approach being unfeasible due to her compromised conditions. The therapy was continued for more than 6 years, and no progression of the disease was observed. During this period, some histological and i...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Noci I,Borri P,Bonfirraro G,Chieffi O,Arcangeli A,Cherubini A,Dabizzi S,Buccoliero AM,Paglierani M,Taddei GL

    更新日期:2001-08-03 00:00:00

  • Thymidylate synthase polymorphisms, folate and B-vitamin intake, and risk of colorectal adenoma.

    abstract::The effects of polymorphisms in genes coding for key folate metabolism enzymes such as thymidylate synthetase (TS) on colorectal neoplasia risk are likely to be influenced by gene-gene and gene-nutrient interactions. We investigated the combined effects of three polymorphisms in the TS gene region, TSER, TS 3R G>C, an...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Hubner RA,Liu JF,Sellick GS,Logan RF,Houlston RS,Muir KR

    更新日期:2007-11-19 00:00:00

  • Serum concentrations of vascular endothelial growth factor and nitrite as an estimate of in vivo nitric oxide in patients with gastric cancer.

    abstract::The importance of tumour angiogenesis in the process of tumour growth and progression in solid tumours has been widely accepted. Among many angiogenic factors, vascular endothelial growth factor (VEGF) has been shown to play a major role in the development and dissemination of the malignant tumours. Nitric oxide (NO) ...

    journal_title:British journal of cancer

    pub_type: 杂志文章


    authors: Eroğlu A,Demirci S,Ayyildiz A,Kocaoğlu H,Akbulut H,Akgül H,Elhan HA

    更新日期:1999-07-01 00:00:00