Abstract:
BACKGROUND:Golgi phosphoprotein 3 (GOLPH3) has been reported to be involved in the development of several human cancers. The present study was conducted to investigate the expression of GOLPH3 and its prognostic significance in renal cell carcinoma (RCC). Meanwhile, the function of GOLPH3 in human RCC was further investigated in cell culture models. METHODS:Expression of GOLPH3 was examined in 43 fresh RCC tissues and paired adjacent normal renal tissues by real-time quantitative PCR and western blotting. Immunohistochemistry for GOLPH3 was performed on additional 218 RCC tissues. The clinical significance of GOLPH3 expression was analysed. Downregulation of GOLPH3 was performed using small-interfering RNA (siRNA) in Caki-1 and 786-O cells with high abundance of GOLPH3, and the effects of GOLPH3 silencing on cell proliferation, migration, invasion in vitro, and tumour growth in vivo were evaluated. RESULTS:Expression of GOLPH3 was upregulated in the majority of the RCC clinical tissue specimens at both mRNA and protein levels. Clinicopathological analysis showed that GOLPH3 expression was significantly correlated with T stage (P<0.001), lymph-node status (P=0.003), distant metastasis (P<0.001), tumour-node-metastasis (TNM) stage (P<0.001), and Fuhman grade (P=0.001). Expression of GOLPH3 was inversely correlated with both overall and recurrence-free survival of RCC patients. Multivariate analysis showed that GOLPH3 expression was an independent prognostic indicator for patient's survival. Knockdown of the GOLPH3 expression reduced cell proliferation, anchorage-independent growth, migration, invasion, and tumour growth in xenograft model mice. CONCLUSIONS:These results suggest that GOLPH3 expression is likely to have important roles in RCC development and progression, and that GOLPH3 is a prognostic biomarker and a promising therapeutic target for RCC.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Xue Y,Wu G,Liao Y,Xiao G,Ma X,Zou X,Zhang G,Xiao R,Wang X,Liu Q,Long D,Yang J,Xu H,Liu F,Liu M,Xie K,Huang Rdoi
10.1038/bjc.2014.124subject
Has Abstractpub_date
2014-04-29 00:00:00pages
2250-60issue
9eissn
0007-0920issn
1532-1827pii
bjc2014124journal_volume
110pub_type
杂志文章abstract:BACKGROUND:The 1997 international consensus conference on renal cell cancer (RCC) prognosis suggested erythrocyte sedimentation rate (ESR), alkaline phosphatase (ALP), and anaemia as prognostic biomarkers, but most studies reviewed were limited by small sample sizes. METHODS:The Cox proportional hazards model was used...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.565
更新日期:2013-02-05 00:00:00
abstract:BACKGROUND:Ovarian and endometrial high-grade serous carcinomas (HGSCs) have similar clinical and pathological characteristics; however, exhaustive protein expression profiling of these cancers has yet to be reported. METHODS:We performed protein expression profiling on 14 cases of HGSCs (7 ovarian and 7 endometrial) ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2016.27
更新日期:2016-03-01 00:00:00
abstract::Chemotherapy has been regarded as standard therapy for the majority of women with advanced epithelial ovarian cancer for several decades, with this role filled largely by the alkylating agents - used as monotherapy - until the mid-1980s. The activity of cisplatin in this disorder was established during the 1970s, and ...
journal_title:British journal of cancer
pub_type: 历史文章,杂志文章,评审
doi:10.1038/sj.bjc.6601494
更新日期:2003-12-01 00:00:00
abstract:BACKGROUND:Hypoxia can activate autophagy, a self-digest adaptive process that maintains cell turnover. Mammalian target of rapamycin (mTOR) inhibitors are used to treat cancer but also stimulate autophagy. METHODS:Human mammary cancer cells and derived xenografts were used to examine whether hypoxia could exacerbate ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.644
更新日期:2013-11-12 00:00:00
abstract::The aim of this study was to examine the effects of oral and transdermal oestrogen replacement therapy on the risk of colorectal cancer. Data from a nested case-control study, designed to investigate the effect of hormone replacement therapy (HRT) on colorectal cancer were analysed. New cases of colorectal cancer, dia...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601438
更新日期:2004-01-12 00:00:00
abstract::Neurofilaments (NFs) are specific intermediate filaments to neural cells. Mammalian NFs are protein triplets composed of three major subunits with respective molecular weights of approximately 70, 150 and 200 kD. Using an immunohistochemical method, 13 carcinoid tumours from different sites were examined for the prese...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1991.161
更新日期:1991-05-01 00:00:00
abstract::This cross-sectional survey aimed to evaluate the prevalence of burnout and estimated psychiatric disorders among haemato-oncology healthcare professionals in Italy. The aspects of work that respondents perceive as stressful and satisfying have also been examined. The assessments were made using the Maslach Burnout In...
journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1038/sj.bjc.6604270
更新日期:2008-03-25 00:00:00
abstract::Phyto-oestrogens are plant compounds structurally similar to oestradiol, which have been proposed to have protective effects against breast cancer. The main class of phyto-oestrogens in the Western diet is lignans. Literature reports on the effect of lignans in breast cancer risk have been conflicting. We performed th...
journal_title:British journal of cancer
pub_type: 杂志文章,meta分析
doi:10.1038/sj.bjc.6605003
更新日期:2009-05-05 00:00:00
abstract:BACKGROUND:LGR5 is an important marker of intestinal stem cells and performs its vital functions at the cell membrane. Despite the importance of LGR5 to both normal and cancer stem cell biology, it is not known how microenvironmental stress affects the expression and subcellular distribution of the protein. METHODS:Nu...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2015.4
更新日期:2015-02-17 00:00:00
abstract::Seventy three children with rhabdomyosarcoma were treated by members of the Children's Solid Tumour Group during the period, 1974-1981. The extent of disease at diagnosis was found to be the major influence affecting outcome. Children with tumours confined to the tissue of origin with no evidence of nodal or metastati...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1983.175
更新日期:1983-08-01 00:00:00
abstract::Spontaneous and radiation-induced genetic instability of peripheral blood mononuclear cells derived from unselected breast cancer (BC) patients (n=50) was examined using the single-cell gel electrophoresis (Comet) assay and a modified G2 micronucleus (MN) test. Cells from apparently healthy donors (n=16) and from canc...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603005
更新日期:2006-04-24 00:00:00
abstract::Even though the majority of patients with bladder malignancies initially present with low stage disease, the clinical epidemiology of these so-called superficial bladder tumours is not well known. In this paper, disease characteristics at initial presentation and during follow-up are described in 1,745 primary cases d...
journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1038/bjc.1993.147
更新日期:1993-04-01 00:00:00
abstract::Cell line studies demonstrate that the PI3K/Akt pathway is upregulated in hormone-refractory prostate cancer (HRPC) and can result in phosphorylation of the androgen receptor (AR). The current study therefore aims to establish if this has relevance to the development of clinical HRPC. Immunohistochemistry was employed...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604152
更新日期:2008-03-25 00:00:00
abstract:BACKGROUND:Childhood cancer survivors can experience learning problems resulting in lower-than-expected attained education as adults. It is unclear whether learning problems manifest already during adolescence. METHODS:We analysed nationwide Danish registries on school grades for Danish children during 2001-2014. Appl...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2016.438
更新日期:2017-01-01 00:00:00
abstract::Patients with advanced malignant disease show an apparent lesser degree of lymphocyte sensitization to cancer antigen when tested under standard conditions than do early cases. In the serum of cancer patients there is a lymphocyte response depressing factor whose titre rises as the neoplasm becomes more extensive. The...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1972.23
更新日期:1972-06-01 00:00:00
abstract::The mechanism of down-regulation of c-myc RNA associated with androgen-induced suppression of the transformed phenotype in the human prostate carcinoma cell line LNCaP was investigated. The synthetic androgen mibolerone (7 alpha-17 alpha-Dimethyl-19-nortestosterone) reversibly inhibits the proliferation of LNCaP cells...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1992.76
更新日期:1992-03-01 00:00:00
abstract::The photodynamic effects of sulphonated aluminium phthalocyanine (SALPC) have been compared on cultured AR4-2J cells of a pancreatic carcinoma cell line and on exocrine cells of the normal phenotype freshly isolated from the rat pancreas; a multi-channel perifusion system was used for this kinetic study in vitro. Wher...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1990.157
更新日期:1990-05-01 00:00:00
abstract::Meningiomas are common, mostly benign, tumours arising from leptomeningeal cells of the meninges, which frequently contain mutations in the neurofibromatosis type 2 (NF2) gene. In this study, we analysed a protein product of the neurofibromatosis type 1 (NF1) gene, neurofibromin, in human established leptomeningeal ce...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.456
更新日期:1997-01-01 00:00:00
abstract::Recent evidence has demonstrated that endothelial-to-mesenchymal transition (EndMT) may have a significant role in a number of diseases. Although EndMT has been previously studied as a critical process in heart development, it is now clear that EndMT can also occur postnatally in various pathologic settings, including...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/sj.bjc.6604662
更新日期:2008-11-04 00:00:00
abstract::Data from the Oxford Survey have shown that childhood cancers are causes of in utero deaths which affect leukaemias more than solid tumours and difficult deliveries more than easy ones. As a result of these biases singletons give the impression of being more leukaemia-sensitive than twins. This is a false impression w...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1973.59
更新日期:1973-06-01 00:00:00
abstract::Disseminating disease is a predictive and prognostic indicator of poor outcome in children with neuroblastoma. Its accurate and sensitive assessment can facilitate optimal treatment decisions. The International Neuroblastoma Risk Group (INRG) Task Force has defined standardised methods for the determination of minimal...
journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究,实务指引
doi:10.1038/sj.bjc.6605029
更新日期:2009-05-19 00:00:00
abstract::Detailed data and statistics per each morphological site of various digestive neoplasms were obtained for the period 1976-87 from the Vaud Cancer Registry datafile, a population-based cancer registration scheme covering about 530,000 inhabitants from the French-speaking part of Switzerland. Tabulations presented inclu...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1991.132
更新日期:1991-04-01 00:00:00
abstract::Apoptosis is a physiological form of cell death important in normal processes such as morphogenesis and the functioning of the immune system. In addition, defects in the apoptotic process play a major role in a number of important areas of disease, such as autoimmune diseases and cancer. DNA-damage-induced apoptosis p...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603257
更新日期:2006-08-21 00:00:00
abstract::Increases of individual beta tubulin isotypes in antimicrotubule drug resistant cell lines have been reported by several laboratories. We have previously described elevations in beta(III)and beta(IVa)isotypes in estramustine and paclitaxel resistant human prostate carcinoma cells. To investigate further the function o...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.2001.1956
更新日期:2001-09-01 00:00:00
abstract::The tumour suppressor gene CDKN2A, located on chromosome 9p21, encodes the cell cycle regulatory protein p16. Inactivation of the CDKN2A gene could lead to uncontrolled cell growth. In order to determine the role of CDKN2A in the development of sporadic ovarian cancer, loss of heterozygosity at 9p21-22, homozygous del...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690621
更新日期:1999-08-01 00:00:00
abstract::Using the breast carcinoma cell line MDA-MB 468 as immunogen, we have produced six new rat monoclonal antibodies (mAbs) against the human EGF receptor (EGFR) and are investigating their use for diagnostic and therapeutic applications in cancer patients whose tumours overexpress these receptors. The mAbs (three IgG2b a...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1993.48
更新日期:1993-02-01 00:00:00
abstract::The incidence of Kaposi's sarcoma (KS) in 1976-90 was assessed in Italy, taking advantage of a network of nine population-based cancer registries covering, at its maximum, approximately 5.6 million subjects. The first examined period (1976-84) substantially reflects the epidemiology of KS prior to the AIDS epidemic in...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1994.60
更新日期:1994-02-01 00:00:00
abstract::Bacteriochlorin a (BCA) is a second-generation photosensitizer that is effective in tumour destruction upon illumination with light of a wavelength of 760 nm. Tissue penetration by light at this wavelength is greater compared with wavelengths at which commonly used photosensitizers are illuminated, making it possible ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1998.353
更新日期:1998-06-01 00:00:00
abstract::The appearance of a liver DNA synthesis promoter (HP) in rat plasma after dimethylnitrosamine (DMNA) or thioacetamide injection was investigated. After 48 h, DMNA (30 mg kg-1 body weight) produced liver (centrilobular) necrosis and intense hepatic regeneration, as assessed by microscopic observations of liver slices, ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1987.122
更新日期:1987-06-01 00:00:00
abstract::Resistance to cytotoxic agents may be encountered during the treatment of acute myeloblastic leukaemia (AML). P-glycoprotein encoded by the MDR-1 gene has been implicated as a potential drug resistance mechanism in leukaemic cells. In recent years, many data have been accrued concerning the expression of P-glycoprotei...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/bjc.1994.70
更新日期:1994-02-01 00:00:00