A human model of xenogeneic graft-versus-host disease in SCID mice engrafted with human peripheral blood lymphocytes.


:Despite previous attempts there is currently no suitable animal model available for xenogeneic graft versus host disease (XGVHD) mediated via human immunocompetent cells. Recently, we have developed an efficient protocol to engraft SCID mice with human peripheral blood lymphocytes (Hu-PBLs). The engraftment efficiency is extremely high, such that 100% of Hu-PBL-SCID mice die of XGVHD within 4 weeks after engraftment with Hu-PBLs (3-5 x 10(7) cells). A series of experiments was performed to investigate the mechanisms involved in the severe XGVHD. The results suggest that XGVHD was induced by human CD4+ T cells, antixenogeneic (antimouse) antibodies, and lymphokines. The SCID mouse model will be extremely valuable for the evaluation and development of immunosuppressive agents and transplantation protocols for human XGVHD.






Sandhu JS,Gorczynski R,Shpitz B,Gallinger S,Nguyen HP,Hozumi N


Has Abstract


1995-07-27 00:00:00












  • Possible role of extracellularly released phagocyte proteinases in coagulation disorder during liver transplantation.

    abstract::Orthotopic liver transplantation is frequently associated with a complex coagulation disorder, influencing the outcome of the procedure. In this respect, disseminated intravascular coagulation (DIC) had been suggested to be of causative importance for bleeding complications after reperfusion of the liver graft. In 10 ...


    pub_type: 杂志文章


    authors: Riess H,Jochum M,Machleidt W,Himmelreich G,Blumhardt G,Roissaint R,Neuhaus P

    更新日期:1991-09-01 00:00:00

  • Immunologic pathways in a quantitative model of immunosuppression based on rejection of an allogeneic or xenogeneic tumor graft.

    abstract:BACKGROUND:A quantitative model of immunosuppression was previously developed based on the rejection of the allogeneic A/J murine tumor sarcoma 1 (Sa1) in immunocompetent mice. Here, the model is used to evaluate the immunologic mechanisms of graft rejection and to determine the potential of this model to detect synerg...


    pub_type: 杂志文章


    authors: Hammond-McKibben D,Saulnier M,Zhang J,Risher N,Lake P,Weetall M

    更新日期:2005-04-27 00:00:00

  • Plasmacytoid Dendritic Cell-driven Induction of Treg Is Strain Specific and Correlates With Spontaneous Acceptance of Kidney Allografts.

    abstract:BACKGROUND:DBA/2J kidney allografts, but not heart allografts, are spontaneously accepted indefinitely in C57BL/6 (B6) mice, through regulatory tolerance mechanism dependent on Foxp3 cells. In contrast, B6 kidneys are rejected within a week in DBA/2J recipients. We hypothesized that the tolerogenic difference of the ki...


    pub_type: 杂志文章


    authors: Oh NA,O'Shea T,Ndishabandi DK,Yuan Q,Hong S,Gans J,Ge J,Gibney S,Chase C,Yang C,Rosales I,Shinoda K,Drew B,Kojima L,Russell PS,Madsen JC,Colvin RB,Alessandrini A

    更新日期:2020-01-01 00:00:00

  • VEGF-A and -C but not -B mediate increased vascular permeability in preserved lung grafts.

    abstract:BACKGROUND:Vascular endothelial growth factor (VEGF) is a potent endothelial cell growth and permeability factor, expressed in the lung. Overexpression of VEGF is associated with increased vascular permeability in the early stage of acute lung injury in mice. The role of various forms of VEGF in transplantation-induced...


    pub_type: 杂志文章


    authors: Abraham D,Taghavi S,Riml P,Paulus P,Hofmann M,Baumann C,Kocher A,Klepetko W,Aharinejad S

    更新日期:2002-06-15 00:00:00

  • Natural Killer Cells: Critical Effectors During Antibody-mediated Rejection of Solid Organ Allografts.

    abstract::Antibody-mediated rejection (AMR) is an important cause of graft loss and continues to present a formidable obstacle to successful transplantation. Unresolved problems continue to be the absence of effective strategies to ablate the donor-specific antibody (DSA) response as well as to attenuate the antibody-mediated g...


    pub_type: 杂志文章


    authors: Miyairi S,Baldwin WM 3rd,Valujskikh A,Fairchild RL

    更新日期:2021-02-01 00:00:00

  • Rat xenograft survival in mice treated with donor-specific transfusion and anti-CD154 antibody is enhanced by elimination of host CD4+ cells.

    abstract:BACKGROUND:Treatment with a donor-specific transfusion (DST) and a brief course of anti-mouse CD154 (anti-CD40-ligand) monoclonal antibody (mAb) prolongs the survival of both allografts and rat xenografts in mice. The mechanism by which allograft survival is prolonged is incompletely understood, but depends in part on ...


    pub_type: 杂志文章


    authors: Gordon EJ,Woda BA,Shultz LD,Rossini AA,Greiner DL,Mordes JP

    更新日期:2001-01-27 00:00:00

  • Rapamycin: immunosuppression, hyporesponsiveness, and side effects in a porcine renal allograft model.

    abstract::Rapamycin prolongs allograft survival and induces donor-specific tolerance in some small animal transplant models. Large animal studies, however, are limited. We studied rapamycin in a porcine renal allograft model. Donor-recipient combinations were chosen based on high response in pretransplant MLCs. Allografts were ...


    pub_type: 杂志文章


    authors: Almond PS,Moss A,Nakhleh RE,Melin M,Chen S,Salazar A,Shirabe K,Matas AJ

    更新日期:1993-08-01 00:00:00

  • Use of renal allografts from donors positive for hepatitis B core antibody confers minimal risk for subsequent development of clinical hepatitis B virus disease.

    abstract:BACKGROUND:The risk associated with transplantation of renal allografts from hepatitis B virus core antibody-positive (HBcAb(+)), hepatitis B virus surface antigen-negative (HBsAg(-)) donors is not well defined. METHODS:Over 4 years, we performed 45 kidney transplants from IgG HBcAb(+), IgM HBcAb(-), HBsAg(-) donors i...


    pub_type: 杂志文章


    authors: Madayag RM,Johnson LB,Bartlett ST,Schweitzer EJ,Constantine NT,McCarter RJ Jr,Kuo PC,Keay S,Oldach DW

    更新日期:1997-12-27 00:00:00

  • Effects of bone marrow-derived natural suppressor activity on B cell responses to lipopolysaccharide.

    abstract::Natural suppressor (NS) activity is detected in tissues undergoing intense hematopoietic regeneration. This includes the spleens of mice recovering from total lymphoid irradiation or cyclophosphamide treatment, or after induction of chronic graft-versus-host disease against minor histocompatibility antigens. NS cells ...


    pub_type: 杂志文章


    authors: Schreiber KL,Forman J

    更新日期:1993-09-01 00:00:00

  • Discriminant quantitation of posttransplant hepatic reticuloendothelial function. The impact of ischemic preservation.

    abstract::This study focuses upon two discrete components of posttransplant hepatic reticuloendothelial system (RES) function-phagocytosis and killing of bacteria-under various conditions of ischemic preservation. We had previously reported that, following intravenous injection of rats with 51Cr and 125I double-labeled Escheric...


    pub_type: 杂志文章


    authors: Klein AS,Zhadkevich M,Wang D,Margolick JB,Winkelstein JA,Bulkley GB

    更新日期:1996-04-27 00:00:00

  • Spontaneous rupture of the liver upon revascularization during transplantation.

    abstract::Spontaneous rupture of the liver has been described in association with many benign and malignant conditions. We report, to our knowledge, the first case of spontaneous rupture of the liver upon revascularization, requiring total hepatectomy and portocaval shunt, followed by successful retransplantation. Routine patho...


    pub_type: 杂志文章,评审


    authors: Mistry BM,Solomon H,Garvin PJ,Durham RM,Turnage S,Bacon BR,Galvin N,Varma CR

    更新日期:2000-05-27 00:00:00

  • De novo sirolimus-based immunosuppression after liver transplantation for hepatocellular carcinoma: long-term outcomes and side effects.

    abstract:BACKGROUND:We report long-term outcomes and side effects after transplantation for hepatocellular carcinoma (HCC) using de novo, sirolimus-based immunosuppression (IS). METHODS:A total of 70 patients with HCC (mean age: 54.4+/-7 years, female/male: 12/58) were transplanted and included in the study. Immunosuppression ...


    pub_type: 杂志文章


    authors: Toso C,Meeberg GA,Bigam DL,Oberholzer J,Shapiro AM,Gutfreund K,Ma MM,Mason AL,Wong WW,Bain VG,Kneteman NM

    更新日期:2007-05-15 00:00:00

  • Immunobiology of natural killer lymphocytes in transplantation.

    abstract::Natural killer (NK) lymphocytes are powerful effector cells of the peripheral immune system. NK cell functions are controlled by the expression of a variety of cell surface receptors with either inhibitory or activating roles. The genetic and functional diversity of this repertoire of receptors and the role of human l...


    pub_type: 杂志文章,评审


    authors: Young NT

    更新日期:2004-07-15 00:00:00

  • The role of graft and host accommodation in a hamster-to-rat cardiac transplantation model.

    abstract:BACKGROUND:We evaluated the importance and mechanism of graft and host accommodation in hamster-to-rat cardiac xenotransplantation models. METHODS:To evaluate graft accommodation, accommodated hamster grafts (Group 2) were transplanted to naïve host rats treated with FK506, and compared with naïve hamster grafts (Grou...


    pub_type: 杂志文章


    authors: Komori K,Fuchimoto Y,Morikawa Y,Obara H,Kawachi S,Tanabe M,Hoshino K,Shimazu M,Matsuzaki Y,Kitajima M

    更新日期:2008-01-15 00:00:00

  • Long-term duct-occluded segmental pancreatic autografts: absence of microvascular diabetic complications.

    abstract:BACKGROUND:Current insulin therapies for control of glucose metabolism in patients with type I diabetes mellitus prevent major metabolic consequences of insulin deficiency, but none prevents or arrests long-term complications. In experimental models of canine diabetes, retinopathy, neuropathy, and nephropathy have been...


    pub_type: 杂志文章


    authors: Hawthorne WJ,Wilson TG,Williamson P,Stewart GJ,Allen RD,Little JM,Deane SA,Ekberg H

    更新日期:1997-10-15 00:00:00

  • Hepatitis C infection, time in renal-replacement therapy, and outcome after kidney transplantation.

    abstract:BACKGROUND:Hepatitis C virus (HCV) infection is common in kidney transplantation and is known to affect long-term patient and graft survival, as is time in renal-replacement therapy (RRT). The aim of this study was to investigate HCV in relation to time in RRT and its impact on outcome after transplantation. METHODS:A...


    pub_type: 杂志文章


    authors: Bruchfeld A,Wilczek H,Elinder CG

    更新日期:2004-09-15 00:00:00

  • Favorable nutritional outcome after isolated liver transplantation for liver failure in a child with short bowel syndrome.

    abstract::A patient with short gut syndrome presented with end-stage liver disease. She underwent an isolated liver transplantation at the age of 3.5 years. Parenteral nutrition was discontinued 1.5 years after surgery. At 7 years of age, the patient has maintained normal nutritional indices and growth while on a normal oral di...


    pub_type: 杂志文章


    authors: Gottrand F,Michaud L,Bonnevalle M,Dubar G,Pruvot FR,Turck D

    更新日期:1999-02-27 00:00:00

  • HLA antibody screening: comparison of a solid phase enzyme-linked immunoassay with antiglobulin-augmented lymphocytotoxicity.

    abstract:BACKGROUND:IgG antibodies to HLA class I antigens can cause hyperacute rejection of renal allografts. Screening of sera from such transplant candidates is laborious, time-consuming, and expensive when performed by sensitive antihuman globulin-augmented lymphocytotoxicity (AHG-CDC). METHODS:Because 60-70% of our transp...


    pub_type: 临床试验,杂志文章


    authors: Moore SB,Ploeger NA,DeGoey SR

    更新日期:1997-12-15 00:00:00

  • Islets in alginate macrobeads reverse diabetes despite minimal acute insulin secretory responses.

    abstract:BACKGROUND:Encapsulation of islets has been widely investigated as a treatment for diabetes. The characteristics and dynamics of insulin secretion by encapsulated islets in response to glucose and other secretagogues are not well understood. METHODS:In our study, macroencapsulated syngeneic islets at 3-4 wk after tran...


    pub_type: 杂志文章


    authors: Trivedi N,Keegan M,Steil GM,Hollister-Lock J,Hasenkamp WM,Colton CK,Bonner-Weir S,Weir GC

    更新日期:2001-01-27 00:00:00

  • Antisense deoxyoligonucleotides or antibodies to murine MD-1 inhibit rejection of allogeneic and xenogeneic skin grafts in C3H mice.

    abstract:BACKGROUND:Altered expression of murine MD-1, a molecule controlling expression of members of the interleukin (IL)-1 receptor family of signaling proteins, regulates antigen-presenting cell-induced alloreactions. We investigated the effect of treatment with antisense deoxyoligonucleotides or antibodies to MD-1 in vivo ...


    pub_type: 杂志文章


    authors: Hadidi S,Chen Z,Phillips J,Yu K,Gorczynski RM

    更新日期:2002-06-15 00:00:00

  • Fifty-percent partial liver transplantation in the rat.

    abstract::Reduced-size liver transplantation in the rat has been useful in the study of hepatic regeneration. We describe a modified technique for partial liver transplantation in the rat using a 50% reduced-size graft. Male Lewis rats (RT1(1)), weighing 250 to 280 g, were used as donors and recipients. The harvested donor live...


    pub_type: 杂志文章


    authors: Omura T,Ascher NL,Emond JC

    更新日期:1996-07-27 00:00:00

  • Outcomes After Declining Increased Infectious Risk Kidney Offers for Pediatric Candidates in the United States.

    abstract:BACKGROUND:Kidneys from infectious risk donors (IRD) confer substantial survival benefit in adults, yet the benefit of IRD kidneys to pediatric candidates remains unclear in the context of high waitlist prioritization. METHODS:Using 2010-2016 Scientific Registry of Transplant Recipients data, we studied 2417 pediatric...


    pub_type: 杂志文章,多中心研究


    authors: Bowring MG,Jackson KR,Wasik H,Neu A,Garonzik-Wang J,Durand C,Desai N,Massie AB,Segev DL

    更新日期:2019-12-01 00:00:00

  • Low-dose mycophenolate mofetil is an effective and safe treatment to permit phased reduction in calcineurin inhibitors in chronic allograft nephropathy.

    abstract:BACKGROUND:Chronic allograft nephropathy (CAN) is the most common cause of long-term renal-allograft dysfunction and the second most common cause of transplant loss. There are concerns that prolonged patient exposure to calcineurin inhibitors (CNIs) exacerbates or promotes the process of CAN. METHODS:In our unit, we c...


    pub_type: 临床试验,杂志文章


    authors: Afzali B,Shah S,Chowdhury P,O'sullivan H,Taylor J,Goldsmith D

    更新日期:2005-02-15 00:00:00

  • Prolongation of renal allograft survival in the rat by pretreatment with donor antigen and cyclosporin A.

    abstract::Pretreatment of LEW rats with cyclosporin A (10 mg/kg/day orally for 14 days) plus 10(8) DA or (DA X LEW)F1 spleen lymphocytes on two occasions markedly prolonged the survival of subsequent DA renal allografts. The lymphocytotoxic antibody response was suppressed completely and renal biopsies showed a marked mononucle...


    pub_type: 杂志文章


    authors: Homan WP,Williams KA,Millard PR,Morris PJ

    更新日期:1981-06-01 00:00:00

  • Treatment of chronic renal allograft rejection in rats with a low-molecular-weight heparin (reviparin).

    abstract:BACKGROUND:Low-molecular-weight heparin (LMWH) has been shown to prolong survival of rat cardiac allografts independently from immunosuppressive treatment. Furthermore, long-term treatment reduces the development of chronic graft vascular disease after experimental heart transplantation. The aim of the present study wa...


    pub_type: 杂志文章


    authors: Braun C,Schultz M,Fang L,Schaub M,Back WE,Herr D,Laux V,Rohmeiss P,Schnuelle P,van der Woude FJ

    更新日期:2001-07-27 00:00:00

  • Renal transplantation from elderly living donors.

    abstract::A worldwide shortage of cadaveric donors has led to the increased utilization of elderly living donors, with controversial results. In an attempt to assess the effect of donor age on graft survival and subsequent renal function, we analyzed our clinical results in 276 consecutive recipients of living related renal tra...


    pub_type: 杂志文章


    authors: Sumrani N,Delaney V,Ding ZK,Davis R,Daskalakis P,Tejani A,Butt K,Hong J

    更新日期:1991-02-01 00:00:00

  • A Feasible Method for Quantifying Living Pancreatic Human Islets in Murine Livers Posttransplantation by Confocal Laser Scanning Microscopy.

    abstract:BACKGROUND:Current histological methods cannot accurately determine the survival rate of human pancreatic islets following portal vein infusion. This is due, in part, to the low number of infused islets relative to the whole liver. In this study, we assessed the ability of confocal laser scanning microscopy (CLSM) to t...


    pub_type: 杂志文章


    authors: Sui F,Tang W,Karunananthan J,Qi C,Li J,He N,Kandeel F,Li J

    更新日期:2020-06-01 00:00:00

  • Normalization of circadian blood pressure profiles after renal transplantation.

    abstract::Most patients with secondary hypertension due to renal disease or on maintenance hemodialysis have lost the physiologic fall of blood pressure during sleep. To test the notion that kidney transplantation normalizes the blood pressure profile, we monitored ambulatory blood pressure over 24 hr in 45 patients (29 males a...


    pub_type: 杂志文章


    authors: Gatzka CD,Schobel HP,Klingbeil AU,Neumayer HH,Schmieder RE

    更新日期:1995-05-15 00:00:00

  • Stimulation of Dopamine D3 Receptor Attenuates Renal Ischemia-Reperfusion Injury via Increased Linkage With Gα12.

    abstract:BACKGROUND:Renal ischemia-reperfusion (I/R) injury causes renal tubular necrosis, apoptosis, and inflammation leading to acute renal dysfunction. Recent studies have revealed that deletion of Gα12 mitigates the renal damage due to I/R injury. Our previous study showed that activation of dopamine D3 receptor (D3R) incre...


    pub_type: 杂志文章


    authors: Wang Z,Guan W,Han Y,Ren H,Tang X,Zhang H,Liu Y,Fu J,He D,Asico LD,Jose PA,Zhou L,Chen L,Zeng C

    更新日期:2015-11-01 00:00:00

  • Donation after circulatory death: current practices, ongoing challenges, and potential improvements.

    abstract::Organ donation after circulatory death (DCD) has been endorsed by the World Health Organization and is practiced worldwide. This overview examines current DCD practices, identifies problems and challenges, and suggests clinical strategies for possible improvement. Although there is uniform agreement on DCD donor candi...


    pub_type: 杂志文章,评审


    authors: Morrissey PE,Monaco AP

    更新日期:2014-02-15 00:00:00