Effects of penicillin, ceftriaxone, and doxycycline on morphology of Borrelia burgdorferi.


:Antibiotic therapy with penicillin, doxycycline, and ceftriaxone has proven to be effective for the treatment of Lyme borreliosis. In some patients, however, it was noticed that borreliae can survival in the tissues in spite of seemingly adequate therapy. For a better understanding of this phenomenon, we investigated the different modes of degeneration of Borrelia burgdorferi suspensions during a 96-h exposure to various antibiotics. By dark-field microscopy and ultrastructural investigations, increasing blebbing and the gradual formation of granular and cystic structures could be followed during the exposure time. Although antibiotic concentrations at the MIC at which 90% of organisms are inhibited after 72 h were 80% or even greater, motile organisms were still present after incubation with penicillin and doxycycline but not after incubation with ceftriaxone. By transmission electron microscopy, intact spirochetal parts, mostly situated in cysts, were seen up to 96 h after exposure with all three antibiotics tested. According to experiences from studies with other spirochetes it is suggested that encysted borreliae, granules, and the remaining blebs might be responsible for the ongoing antigenic stimulus leading to complaints of chronic Lyme borreliosis.


Kersten A,Poitschek C,Rauch S,Aberer E




Has Abstract


1995-05-01 00:00:00












  • Antibiotic susceptibility of enterohemorrhagic Escherichia coli O157:H7 isolated from an outbreak in Japan in 1996.

    abstract::The antibiotic susceptibilities of 43 strains of Escherichia coli O157:H7 identified in the summer of 1996 in Japan were investigated. Growth of 90% of O157 strains was inhibited at a concentration of < or = 0.5 micro/ml by several agents including fosfomycin with glucose-6-phosphate. ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Tsuboi I,Ida H,Yoshikawa E,Hiyoshi S,Yamaji E,Nakayama I,Nonomiya T,Shigenobu F,Shimizu M,O'Hara K,Sawai T,Mizuoka K

    更新日期:1998-02-01 00:00:00

  • Antibacterial activity and mechanism of action of a novel anilinouracil-fluoroquinolone hybrid compound.

    abstract::The anilinouracils (AUs) such as 6-(3-ethyl-4-methylanilino)uracil (EMAU) are a novel class of gram-positive, selective, bactericidal antibacterials which inhibit pol IIIC, the gram-positive-specific replicative DNA polymerase. We have linked various fluoroquinolones (FQs) to the N-3 position of EMAU to generate a var...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Butler MM,Lamarr WA,Foster KA,Barnes MH,Skow DJ,Lyden PT,Kustigian LM,Zhi C,Brown NC,Wright GE,Bowlin TL

    更新日期:2007-01-01 00:00:00

  • Bioavailability of oral trimetrexate in patients with acquired immunodeficiency syndrome.

    abstract::The combination of the lipophilic antifolate trimetrexate and the rescue agent leucovorin has shown promise in the treatment of Pneumocystis carinii pneumonia in patients with acquired immunodeficiency syndrome. The pharmacokinetic behavior of trimetrexate administered either by intravenous bolus or orally was studied...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Rogers P,Allegra CJ,Murphy RF,Drake JC,Masur H,Poplack DG,Chabner BA,Parrillo JE,Lane HC,Balis FM

    更新日期:1988-03-01 00:00:00

  • CNI-H0294, a nuclear importation inhibitor of the human immunodeficiency virus type 1 genome, abrogates virus replication in infected activated peripheral blood mononuclear cells.

    abstract::Active nuclear importation of the human immunodeficiency virus (HIV) type 1 (HIV-1) preintegration complex (PIC) is required for the productive infection of nondividing cells, but it is believed to be dispensable for the infection of proliferating cells, such as activated T lymphocytes. To investigate this question, w...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Haffar OK,Smithgall MD,Popov S,Ulrich P,Bruce AG,Nadler SG,Cerami A,Bukrinsky MI

    更新日期:1998-05-01 00:00:00

  • Comparison of antiviral activities of cloned and native human interferons against herpes simplex virus types 1 and 2 and human cytomegalovirus.

    abstract::Five recombinant alpha interferons and two recombinant beta interferons have been tested for their ability to inhibit yields of herpes simplex virus types 1 and 2 and human cytomegalovirus in human embryonic lung cells. All of the alpha species and both of the beta forms (cysteine and serine) were active against the h...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Rasmussen LE,Chen PT,Merigan TC

    更新日期:1984-10-01 00:00:00

  • In vitro studies of the combined effect of ampicillin and sulfonamides on Nocardia asteroides and results of therapy in four patients.

    abstract::The minimal inhibiting concentrations (MIC) of ampicillin, trisulfapyrimidines, and various combinations of these drugs were determined for four clinical isolates of Nocardia asteroides on Mueller-Hinton agar. The combination of ampicillin and sulfonamides was found to be synergistic for three of the four isolates whe...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Orfanakis MG,Wilcox HG,Smith CB

    更新日期:1972-03-01 00:00:00

  • Azlocillin pharmacokinetics in patients with cystic fibrosis.

    abstract::The pharmacokinetics of azlocillin were studied in 10 cystic fibrosis patients, ranging in age from 11 to 28 years. The patients received a 9- to 23-day course of 350 mg of azlocillin per kg in four or six divided daily doses in combination with am aminoglycoside. Blood and urine samples were collected at specified ti...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Bosso JA,Saxon BA,Herbst JJ,Matsen JM

    更新日期:1984-05-01 00:00:00

  • Updated functional classification of beta-lactamases.

    abstract::Two classification schemes for beta-lactamases are currently in use. The molecular classification is based on the amino acid sequence and divides beta-lactamases into class A, C, and D enzymes which utilize serine for beta-lactam hydrolysis and class B metalloenzymes which require divalent zinc ions for substrate hydr...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章,评审


    authors: Bush K,Jacoby GA

    更新日期:2010-03-01 00:00:00

  • In vitro antifungal susceptibilities of Sporothrix schenckii in two growth phases.

    abstract::We have determined the antifungal susceptibilities of 34 clinical isolates of the dimorphic fungus Sporothrix schenckii to 11 drugs using a microdilution method. In general, the type of growth phase (mycelial or yeast) and the temperature of incubation (30 or 35 degrees C) exerted a significant influence on the MICs. ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Trilles L,Fernández-Torres B,Dos Santos Lazéra M,Wanke B,de Oliveira Schubach A,de Almeida Paes R,Inza I,Guarro J

    更新日期:2005-09-01 00:00:00

  • Regulation and Anaerobic Function of the Clostridioides difficile β-Lactamase.

    abstract::Clostridioides difficile causes severe antibiotic-associated diarrhea and colitis. C. difficile is an anaerobic, Gram-positive sporeformer that is highly resistant to β-lactams, the most commonly prescribed antibiotics. The resistance of C. difficile to β-lactam antibiotics allows the pathogen to replicate and cause d...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Sandhu BK,Edwards AN,Anderson SE,Woods EC,McBride SM

    更新日期:2019-12-20 00:00:00

  • Antibiofilm Activity and Synergistic Inhibition of Staphylococcus aureus Biofilms by Bactericidal Protein P128 in Combination with Antibiotics.

    abstract::P128 is an antistaphylococcal protein, comprising a cell wall-degrading enzymatic region and a Staphylococcus-specific binding region, which possesses specific and potent bactericidal activity against sensitive and drug-resistant strains of Staphylococcus aureus To explore P128's ability to kill S. aureus in a range o...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Nair S,Desai S,Poonacha N,Vipra A,Sharma U

    更新日期:2016-11-21 00:00:00

  • Penetration of oral fluconazole into gynecological tissues.

    abstract::Penetration of fluconazole into female genital tissues was examined. Fluconazole was administered orally at a dose of 150 mg to patients undergoing total abdominal hysterectomy 1 to 151 h prior to surgery. During surgery, blood, uterus, ovary, and oviduct were sampled. Fluconazole concentrations in each tissue were de...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 临床试验,杂志文章


    authors: Mikamo H,Kawazoe K,Sato Y,Izumi K,Ito T,Ito K,Tamaya T

    更新日期:1999-01-01 00:00:00

  • Standardization of disk diffusion test and its clinical significance for susceptibility testing of metronidazole against Helicobacter pylori.

    abstract::Susceptibilities of 121 clinical Helicobacter pylori strains to metronidazole were determined by both a 5-micrograms metronidazole disk diffusion test and a plate dilution method in duplicate and after different periods of incubation. The distribution of MICs of metronidazole against H. pylori among the strains was fo...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 临床试验,杂志文章


    authors: Xia H,Keane CT,Beattie S,O'Morain CA

    更新日期:1994-10-01 00:00:00

  • Surveillance of community-based reservoirs reveals the presence of CTX-M, imported AmpC, and OXA-30 beta-lactamases in urine isolates of Klebsiella pneumoniae and Escherichia coli in a U.S. community.

    abstract::beta-Lactamases produced by urine isolates from patients in long-term care facilities (LTCFs), outpatient, clinics, and one hospital in a U.S. community were characterized. A total of 1.3% of all Escherichia coli and Klebsiella pneumoniae isolates collected from patients in 30 LTCFs and various outpatient clinics prod...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Hanson ND,Moland ES,Hong SG,Propst K,Novak DJ,Cavalieri SJ

    更新日期:2008-10-01 00:00:00

  • High-level tetracycline resistance resulting from TetM in strains of Neisseria spp., Kingella denitrificans, and Eikenella corrodens.

    abstract::Similar to Neisseria gonorrhoeae, tetracycline-resistant isolates of N. meningitidis, Kingella denitrificans, and Eikenella corrodens contained 25.2-megadalton plasmids carrying the TetM determinant. In contrast, tetracycline-resistant N. subflava biovar perflava-N. sicca and N. mucosa isolates carried the TetM determ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Knapp JS,Johnson SR,Zenilman JM,Roberts MC,Morse SA

    更新日期:1988-05-01 00:00:00

  • Susceptibility of Mycobacterium kansasii to ofloxacin, sparfloxacin, clarithromycin, azithromycin, and fusidic acid.

    abstract::The MICs of ofloxacin, sparfloxacin, clarithromycin, azithromycin, and fusidic acid for clinical isolates of Mycobacterium kansasii were determined by the radiometric (BACTEC) method. All drugs except azithromycin elicited MICs for 90% of the strains tested that were lower than previously reported achievable maximum c...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Witzig RS,Franzblau SG

    更新日期:1993-09-01 00:00:00

  • Antifungal activities of natural and synthetic iron chelators alone and in combination with azole and polyene antibiotics against Aspergillus fumigatus.

    abstract::Antifungal effects of iron chelators (lactoferrin, deferoxamine, deferiprone, and ciclopirox) were tested alone and in combination with antifungal drugs against Aspergillus fumigatus B5233 conidia. Lactoferrin, ciclopirox, and deferiprone inhibited whereas deferoxamine enhanced fungal growth. Antifungal synergy agains...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Zarember KA,Cruz AR,Huang CY,Gallin JI

    更新日期:2009-06-01 00:00:00

  • In Vitro Activity of Ceftaroline against Staphylococcus aureus Isolates Collected in 2012 from Latin American Countries as Part of the AWARE Surveillance Program.

    abstract::The in vitro activities of ceftaroline and comparators, using broth microdilution, were determined against 1,066 Staphylococcus aureus isolates from hospitalized patients. Seventeen medical centers from Latin American countries contributed isolates. Methicillin-resistant S. aureus (MRSA) percentages ranged from 46% (B...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章,多中心研究


    authors: Biedenbach DJ,Hoban DJ,Reiszner E,Lahiri SD,Alm RA,Sahm DF,Bouchillon SK,Ambler JE

    更新日期:2015-12-01 00:00:00

  • Frequency of voriconazole resistance in vitro among Spanish clinical isolates of Candida spp. According to breakpoints established by the Antifungal Subcommittee of the European Committee on Antimicrobial Susceptibility Testing.

    abstract::A total of 4,226 Spanish clinical isolates of Candida spp. were analyzed to assess resistance to voriconazole according to breakpoints established by the European Committee for Antimicrobial Susceptibility Testing (where susceptibility [S] to voriconazole corresponds to a MIC of ≤ 0.12 mg/liter). Resistance was uncomm...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Cuenca-Estrella M,Gomez-Lopez A,Cuesta I,Zaragoza O,Mellado E,Rodriguez-Tudela JL,Antifungal Subcommittee of European Committee on Antimicrobial Susceptibility Testing.

    更新日期:2011-04-01 00:00:00

  • The complete nucleotide sequence of the carbapenem resistance-conferring conjugative plasmid pLD209 from a Pseudomonas putida clinical strain reveals a chimeric design formed by modules derived from both environmental and clinical bacteria.

    abstract::The complete sequence of the carbapenem-resistance-conferring conjugative plasmid pLD209 from a Pseudomonas putida clinical strain is presented. pLD209 is formed by 3 well-defined regions: an adaptability module encompassing a Tn402-like class 1 integron of clinical origin containing blaVIM-2 and aacA4 gene cassettes,...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Marchiaro PM,Brambilla L,Morán-Barrio J,Revale S,Pasteran F,Vila AJ,Viale AM,Limansky AS

    更新日期:2014-01-01 00:00:00

  • Efficacies of vancomycin, arbekacin, and gentamicin alone or in combination against methicillin-resistant Staphylococcus aureus in an in vitro infective endocarditis model.

    abstract::We adopted an in vitro infective endocarditis model (IVIEM) to compare the efficacy of vancomycin (VAN), arbekacin (ABK), and gentamicin (GEN) alone or in combination. Using two strains of clinically isolated methicillin-resistant Staphylococcus aureus, one GEN susceptible (GS171) and one GEN resistant (GR153), fibrin...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Lee DG,Chun HS,Yim DS,Choi SM,Choi JH,Yoo JH,Shin WS,Kang MW

    更新日期:2003-12-01 00:00:00

  • Clinical trial of piperacillin with acquisition of resistance by Pseudomonas and clinical relapse.

    abstract::A total of 20 serious infections were treated with piperacillin. These infections included bacteremias (5), pneumonias (5), urinary tract infections (5), soft tissue infections (3), septic arthritis (1), and osteomyelitis (1). The most common bacterial pathogen was Pseudomonas aeruginosa, accounting for eight infectio...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 临床试验,杂志文章


    authors: Simon GL,Snydman DR,Tally FP,Gorbach SL

    更新日期:1980-07-01 00:00:00

  • Interspecies dissemination of the bla gene encoding PER-1 extended-spectrum β-lactamase.

    abstract::PER-1 extended-spectrum β-lactamase-producing Gram-negative bacilli are resistant to oxyimino-cephalosporins. However, the bla(PER-1) gene has never been reported in Klebsiella pneumoniae. Here, we studied interspecies dissemination of the bla(PER-1) gene by horizontal transfer of Tn1213 among Acinetobacter baumannii,...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Bae IK,Jang SJ,Kim J,Jeong SH,Cho B,Lee K

    更新日期:2011-03-01 00:00:00

  • Low Tenofovir Plasma Exposure in HIV Oral Pre-exposure Prophylaxis Recipients with Gastrointestinal Disorders.

    abstract::Four pre-exposure prophylaxis (PrEP) users with gastro-intestinal disorders (sleeve gastrectomy, terminal ileitis, celiac disease or chronic diarrhea) and receiving oral tenofovir disoproxil fumarate/emtricitabine (TDF/FTC) were included. Despite a self-reported high adherence, trough plasma tenofovir concentrations (...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Calcagno A,Dal Conte I,Cattaneo D,Testi R,Mistrangelo M,Gervasoni C,de Nicolò A,Bonora S,D'Avolio A,Di Perri G

    更新日期:2020-10-26 00:00:00

  • In vitro comparison of ampicillin-chloramphenicol and ampicillin-cefotaxime against 284 Haemophilus isolates.

    abstract::Since November 1982 at the Sainte-Justine Hospital in Montreal, ampicillin and cefotaxime were used in association as initial treatment (greater than or equal to 48 h) for childhood bacterial meningitis. In this report is described the in vitro interaction of the new regimen in comparison with that of the previous amp...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Lapointe JR,Lavallée C,Michaud A,Chicoine L,Joncas JH

    更新日期:1986-04-01 00:00:00

  • Inhibitors of sterol biosynthesis and amphotericin B reduce the viability of pneumocystis carinii f. sp. carinii.

    abstract::Pneumocystis carinii synthesizes sterols with a double bond at C-7 of the sterol nucleus and an alkyl group with one or two carbons at C-24 of the side chain. Also, some human-derived Pneumocystis carinii f. sp. hominis strains contain lanosterol derivatives with an alkyl group at C-24. These unique sterols have not b...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Kaneshiro ES,Collins MS,Cushion MT

    更新日期:2000-06-01 00:00:00

  • Prevention of experimental endotoxin shock by a monocyte activator.

    abstract::In patients with polytrauma or major surgery, severe bacterial infections leading to septic shock and multiorgan failure are still a major cause of death. Prevention of septic shock in patients at risk would be an alternative to treatment of patients with overt septic shock. We therefore conducted a trial with the mon...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Passlick B,Labeta MO,Izbicki JR,Ostertag P,Löffler T,Siebeck M,Pichlmeier U,Schweiberer L,Ziegler-Heitbrock HW

    更新日期:1995-11-01 00:00:00

  • RFI-641, a potent respiratory syncytial virus inhibitor.

    abstract::Human respiratory syncytial virus (RSV), a paramyxovirus, is a major cause of acute upper and lower respiratory tract infections in infants, young children, and adults. RFI-641 is a novel anti-RSV agent with potent in vitro and in vivo activity. RFI-641 is active against both RSV type A and B strains. The viral specif...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Huntley CC,Weiss WJ,Gazumyan A,Buklan A,Feld B,Hu W,Jones TR,Murphy T,Nikitenko AA,O'Hara B,Prince G,Quartuccio S,Raifeld YE,Wyde P,O'Connell JF

    更新日期:2002-03-01 00:00:00

  • Isolating specific auxotrophic mutants of Mycobacterium smegmatis by using vancomycin.

    abstract::After inducing mutagenesis with nitrosoguanidine, we used vancomycin enrichment in isolating auxotrophic mutants of Mycobacterium smegmatis. Compared with cycloserine and penicillin G, which are also cell wall inhibitors, vancomycin, with 24 h of exposure, produced less cell lysis and a greater reduction in viable cel...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Woodley CL,Baldwin JN,Greenberg J

    更新日期:1981-04-01 00:00:00

  • Synergistic activities of fortimicin A and beta-lactam antibiotics against Pseudomonas aeruginosa.

    abstract::The inhibitory and bactericidal activities of fortimicin A (FTM-A) alone against Pseudomonas aeruginosa were compared with those of FTM-A in combination with beta-lactam antibiotics. As tested by the checkerboard method, most beta-lactam antibiotics tested had synergistic effects on the inhibitory activity of FTM-A ag...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Yamashita K,Kawabe H,Mitsuhashi S

    更新日期:1981-07-01 00:00:00