In vivo microscopy of the cerebral microcirculation using neonatal allografts in hamsters.


:Studies were performed to characterize the morphology and vascular reactivity of the allografted cerebral microcirculation. Cerebral cortical tissue was allografted into the cheek pouch of the hamster so that cerebral parenchymal vessels could be studied. The vascular morphology was characterized by a large number of looping vessels. The ultrastructural examination indicated viable cerebral tissue containing typical vessels, that is, "tight" junctions, not like those of the cheek pouch. Also, the microvasculature was impermeable to 150, 70, and 20 kDa fluorescein isothiocyanate dextrans. Angiotensin II and norepinephrine caused constriction of the cerebral vessels whereas adenosine caused dilation. Isoproterenol did not affect cerebral arterioles; however, it dilated cheek pouch arterioles. Thus, this preparation provides a satisfactory model for studying the living cerebral microcirculation.


Circ Res


Circulation research


Joyner WL,Young R,Blank D,Eccleston-Joyner CA,Gilmore JP




Has Abstract


1988-10-01 00:00:00












  • Measurement by fluorescence of interstitial adenosine levels in normoxic, hypoxic, and ischemic perfused rat hearts.

    abstract::An improved assay was used to investigate the effects of hypoxia or ischemia on interstitial fluid and coronary venous effluent levels of adenosine in isolated perfused nonworking rat hearts. The adenosine in 5- to 10-microliter samples of left ventricular epicardial surface transudates and coronary effluents was reac...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Fenton RA,Dobson JG Jr

    更新日期:1987-02-01 00:00:00

  • Comparison of the effects of different inotropic interventions on force, velocity, and power in rabbit myocardium.

    abstract::To gain some insight into inotropic mechanisms, we compared the effects of several classes of inotropic interventions on the isometric twitch and force-velocity properties of isolated rabbit myocardium. Postextrasystolic potentiation was chosen as one of the interventions in the belief that its onset is so rapid that ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Chiu YC,Walley KR,Ford LE

    更新日期:1989-11-01 00:00:00

  • Ca(2+) transients and Ca(2+) waves in purkinje cells : role in action potential initiation.

    abstract::Purkinje cells contain sarcoplasmic reticulum (SR) directly under the surface membrane, are devoid of t-tubuli, and are packed with myofibrils surrounded by central SR. Several studies have reported that electrical excitation induces a biphasic Ca(2+) transient in Purkinje fiber bundles. We determined the nature of th...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Boyden PA,Pu J,Pinto J,Keurs HE

    更新日期:2000-03-03 00:00:00

  • Three distinct types of Ca(2+) waves in Langendorff-perfused rat heart revealed by real-time confocal microscopy.

    abstract::Although Ca(2+) waves in cardiac myocytes are regarded as arrhythmogenic substrates, their properties in the heart in situ are poorly understood. On the hypothesis that Ca(2+) waves in the heart behave diversely and some of them influence the cardiac function, we analyzed their incidence, propagation velocity, and int...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Kaneko T,Tanaka H,Oyamada M,Kawata S,Takamatsu T

    更新日期:2000-05-26 00:00:00

  • Glucocorticoid induction of Kv1.5 K+ channel gene expression in ventricle of rat heart.

    abstract::Multiple voltage-gated K+ channels contribute to the repolarization phases of the cardiac action potential and are targets of several antiarrhythmic drugs. The Kv1.5 K+ channel gene is expressed in the heart, and heterologous expression of this gene generates a slowly inactivating K+ current. Previously, we found that...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Takimoto K,Levitan ES

    更新日期:1994-12-01 00:00:00

  • Heparin increases exercise-induced collateral blood flow in rats with femoral artery ligation.

    abstract::The potential for heparin to enhance the training-induced increase in collateral-dependent blood flow to the distal hind-limb muscles was evaluated after bilateral femoral artery ligation in adult male rats (approximately 350 g). Rats received either saline (n = 34) or heparin (n = 36) injections and were kept sedenta...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Yang HT,Ogilvie RW,Terjung RL

    更新日期:1995-03-01 00:00:00

  • 14,15-Epoxyeicosa-5(Z)-enoic acid: a selective epoxyeicosatrienoic acid antagonist that inhibits endothelium-dependent hyperpolarization and relaxation in coronary arteries.

    abstract::Endothelium-dependent hyperpolarization and relaxation of vascular smooth muscle are mediated by endothelium-derived hyperpolarizing factors (EDHFs). EDHF candidates include cytochrome P-450 metabolites of arachidonic acid, K(+), hydrogen peroxide, or electrical coupling through gap junctions. In bovine coronary arter...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Gauthier KM,Deeter C,Krishna UM,Reddy YK,Bondlela M,Falck JR,Campbell WB

    更新日期:2002-05-17 00:00:00

  • Beta-adrenergic receptor-stimulated apoptosis in cardiac myocytes is mediated by reactive oxygen species/c-Jun NH2-terminal kinase-dependent activation of the mitochondrial pathway.

    abstract::Stimulation of beta-adrenergic receptors (betaARs) causes apoptosis in adult rat ventricular myocytes (ARVMs). The role of reactive oxygen species (ROS) in mediating betaAR-stimulated apoptosis is not known. Stimulation of betaARs with norepinephrine (10 micromol/L) in the presence of prazosin (100 nmol/L) for 24 hour...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Remondino A,Kwon SH,Communal C,Pimentel DR,Sawyer DB,Singh K,Colucci WS

    更新日期:2003-02-07 00:00:00

  • Sarco/endoplasmic reticulum Ca2+-ATPase gene transfer reduces vascular smooth muscle cell proliferation and neointima formation in the rat.

    abstract::Proliferation of vascular smooth muscle cells (VSMC) is a primary cause of vascular disorders and is associated with major alterations in Ca2+ handling supported by loss of the sarco/endoplasmic reticulum calcium ATPase, SERCA2a. To determine the importance of SERCA2a in neointima formation, we have prevented loss of ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Lipskaia L,del Monte F,Capiod T,Yacoubi S,Hadri L,Hours M,Hajjar RJ,Lompré AM

    更新日期:2005-09-02 00:00:00

  • Mechanical interactions between four heart chambers with and without the pericardium in canine hearts.

    abstract::By using excised postmortem hearts obtained from 15 mongrel dogs with the pericardium intact, we investigated mechanical interactions between the four heart chambers from the standpoint of ventricular pressure-volume relationships. The interactions investigated were those between (1) the atrium and the ventricle, (2) ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Maruyama Y,Ashikawa K,Isoyama S,Kanatsuka H,Ino-Oka E,Takishima T

    更新日期:1982-01-01 00:00:00

  • The intersection between aging and cardiovascular disease.

    abstract::The average lifespan of humans is increasing, and with it the percentage of people entering the 65 and older age group is growing rapidly and will continue to do so in the next 20 years. Within this age group, cardiovascular disease will remain the leading cause of death, and the cost associated with treatment will co...

    journal_title:Circulation research

    pub_type: 杂志文章,评审


    authors: North BJ,Sinclair DA

    更新日期:2012-04-13 00:00:00

  • Phosphoinositide-dependent kinase 1 and p21-activated protein kinase mediate reactive oxygen species-dependent regulation of platelet-derived growth factor-induced smooth muscle cell migration.

    abstract::Smooth muscle cell migration in response to platelet-derived growth factor (PDGF) is a key event in several vascular pathologies, including atherosclerosis and restenosis. PDGF increases intracellular levels of reactive oxygen species (ROS) in vascular smooth muscle cells (VSMCs), but the ROS sensitivity of migration ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Weber DS,Taniyama Y,Rocic P,Seshiah PN,Dechert MA,Gerthoffer WT,Griendling KK

    更新日期:2004-05-14 00:00:00

  • Sphingosylphosphorylcholine induces a hypertrophic growth response through the mitogen-activated protein kinase signaling cascade in rat neonatal cardiac myocytes.

    abstract::The sphingolipid metabolites, sphingosine (SPH), SPH 1-phosphate (S1P), and sphingosylphosphorylcholine (SPC), can act as intracellular as well as extracellular signaling molecules. These compounds have been implicated in the regulation of cell growth, differentiation, and programmed cell death in nonmyocytes, but the...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Sekiguchi K,Yokoyama T,Kurabayashi M,Okajima F,Nagai R

    更新日期:1999-11-26 00:00:00

  • Angiogenesis and pericytes in the initiation of ectopic calcification.

    abstract::Ectopic calcification of blood vessels, heart valves, and skeletal muscle is a major clinical problem. There is now good evidence that angiogenesis is associated with ectopic calcification in these tissues and that it is necessary, but not sufficient, for calcification to occur. Angiogenesis may regulate ectopic calci...

    journal_title:Circulation research

    pub_type: 杂志文章,评审


    authors: Collett GD,Canfield AE

    更新日期:2005-05-13 00:00:00

  • Notch signaling regulates platelet-derived growth factor receptor-beta expression in vascular smooth muscle cells.

    abstract::Notch signaling is critically important for proper architecture of the vascular system, and mutations in NOTCH3 are associated with CADASIL, a stroke and dementia syndrome with vascular smooth muscle cell (VSMC) dysfunction. In this report, we link Notch signaling to platelet-derived growth factor (PDGF) signaling, a ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Jin S,Hansson EM,Tikka S,Lanner F,Sahlgren C,Farnebo F,Baumann M,Kalimo H,Lendahl U

    更新日期:2008-06-20 00:00:00

  • Regulation of calcium current by intracellular calcium in smooth muscle cells of rabbit portal vein.

    abstract::Effects of concentrations of intracellular calcium, [Ca2+]i, on the voltage-dependent Ca2+ current (ICa) recorded from dispersed single smooth muscle cells of the rabbit portal vein were studied, using a whole cell voltage clamp method combined with an intracellular perfusion technique. Outward currents were minimized...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Ohya Y,Kitamura K,Kuriyama H

    更新日期:1988-02-01 00:00:00

  • An oscillatory current in sheep cardiac Purkinje fibers.

    abstract::The inward current ("oscillatory current") which may be present after the end of a depolarizing clamp was studied in sheep cardiac Purkinje fibers by means of a voltage-clamp method. The following results were obtained. In order to appear, the oscillatory current (Ios) requires a previous depolarization to approximate...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Vassalle M,Mugelli A

    更新日期:1981-05-01 00:00:00

  • Functional and morphological organization of the rabbit sinus node.

    abstract::In isolated right atria of the rabbit heart, we studied the activation pattern within the sinus node, using the microelectrode technique. After the electrophysiological experiments, the preparations were subjected to a correlative morphological investigation, using light or electron microscopy. Different criteria for ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Bleeker WK,Mackaay AJ,Masson-Pévet M,Bouman LN,Becker AE

    更新日期:1980-01-01 00:00:00

  • Mechanisms of TGF-beta1-induced intimal growth: plasminogen-independent activities of plasminogen activator inhibitor-1 and heterogeneous origin of intimal cells.

    abstract::Transforming growth factor (TGF)-beta(1) is a potent stimulator of intimal growth. We showed previously that TGF-beta(1) stimulates intimal growth through early upregulation of plasminogen activator inhibitor-1 (PAI-1) and, subsequently, PAI-1-dependent increases in cell migration and matrix accumulation. We also show...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Otsuka G,Stempien-Otero A,Frutkin AD,Dichek DA

    更新日期:2007-05-11 00:00:00

  • Cytokine-Like 1 Is a Novel Proangiogenic Factor Secreted by and Mediating Functions of Endothelial Progenitor Cells.

    abstract:RATIONALE:Endothelial colony forming cells (ECFCs) or late blood outgrowth endothelial cells can be isolated from human cord or peripheral blood, display properties of endothelial progenitors, home into ischemic tissues and support neovascularization in ischemic disease models. OBJECTIVE:To assess the functions of CYT...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Schneller D,Hofer-Warbinek R,Sturtzel C,Lipnik K,Gencelli B,Seltenhammer M,Wen M,Testori J,Bilban M,Borowski A,Windwarder M,Kapel SS,Besemfelder E,Cejka P,Habertheuer A,Schlechta B,Majdic O,Altmann F,Kocher A,August

    更新日期:2019-01-18 00:00:00

  • Hypertension, transmural pressure, and vascular smooth muscle response in rats.

    abstract::The effect of transmural pressure on the responsiveness of vascular smooth muscle was studied using rats with chronic occlusion of one external iliac artery. The arterial pressure in the occluded leg was reduced to approximately half of that in the contralateral unoccluded leg. Helical strips from the low- and high-pr...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Hansen TR,Bohr DF

    更新日期:1975-05-01 00:00:00

  • Atrial natriuretic peptide in chronic heart failure in the rat: a correlation with ventricular dysfunction.

    abstract::To assess the relation between atrial natriuretic peptide and ventricular dysfunction, we simultaneously measured both atrial and plasma immunoreactive atrial natriuretic peptide concentrations in rats 4 weeks after myocardial infarction induced by left coronary artery ligation. When compared to controls (n = 39), rat...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Tsunoda K,Hodsman GP,Sumithran E,Johnston CI

    更新日期:1986-09-01 00:00:00

  • Electrophysiology and ultrastructure of canine subendocardial Purkinje cells isolated from control and 24-hour infarcted hearts.

    abstract::Ventricular arrhythmias that accompany myocardial infarction in dogs may be secondary to the altered electrophysiological properties of the subendocardial Purkinje fibers that survive 24 hours after the coronary occlusion. To better understand the ionic mechanisms that underlie the altered electrical activity of these...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Boyden PA,Albala A,Dresdner KP Jr

    更新日期:1989-10-01 00:00:00

  • Thin filament mutations: developing an integrative approach to a complex disorder.

    abstract::Sixteen years ago, mutations in cardiac troponin (Tn)T and α-tropomyosin were linked to familial hypertrophic cardiomyopathy, thus transforming the disorder from a disease of the β-myosin heavy chain to a disease of the cardiac sarcomere. From the outset, studies suggested that mutations in the regulatory thin filamen...

    journal_title:Circulation research

    pub_type: 杂志文章,评审


    authors: Tardiff JC

    更新日期:2011-03-18 00:00:00

  • Role of nitric oxide and cyclic GMP as mediators of endothelium-independent neurogenic relaxation in bovine mesenteric artery.

    abstract::Electrical field stimulation (EFS) of phenylephrine-contracted bovine mesenteric arteries pretreated with guanethidine elicited a relaxation that amounted to roughly 40%. This relaxation was sensitive to tetrodotoxin pretreatment, suggesting a neurogenic origin. The EFS-induced relaxation was correlated to an increase...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Ahlner J,Ljusegren ME,Grundström N,Axelsson KL

    更新日期:1991-03-01 00:00:00

  • Hypoxic induction of the hypoxia-inducible factor is mediated via the adaptor protein Shc in endothelial cells.

    abstract::Tyrosine kinase cascades may play a role in the hypoxic regulation of hypoxia-inducible factor (HIF)-1. We investigated the role of tyrosine kinase phosphorylation and of the Shc/Ras cascade on hypoxic HIF-1 stabilization. Exposure of human umbilical vein endothelial cells to hypoxia results in HIF protein stabilizati...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Jung F,Haendeler J,Hoffmann J,Reissner A,Dernbach E,Zeiher AM,Dimmeler S

    更新日期:2002-07-12 00:00:00

  • AMPKα2 Protects Against the Development of Heart Failure by Enhancing Mitophagy via PINK1 Phosphorylation.

    abstract:RATIONALE:Mitochondrial dysfunction plays an important role in heart failure (HF). However, the molecular mechanisms regulating mitochondrial functions via selective mitochondrial autophagy (mitophagy) are poorly understood. OBJECTIVE:We sought to determine the role of AMPK (AMP-activated protein kinase) in selective ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Wang B,Nie J,Wu L,Hu Y,Wen Z,Dong L,Zou MH,Chen C,Wang DW

    更新日期:2018-03-02 00:00:00

  • Mechanism of adenosine-induced inhibition of calcium current in guinea pig ventricular cells.

    abstract::The mechanism of adenosine-induced inhibition of Ca2+ currents was studied by recording single-channel Ca2+ currents from cell-attached patches on isolated guinea pig ventricular cells with pipettes containing 50 or 100 mM Ba2+. Numerous 100-msec depolarization steps were applied repetitively at 2 Hz from the resting ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Kato M,Yamaguchi H,Ochi R

    更新日期:1990-11-01 00:00:00

  • Increased sarcoplasmic reticulum calcium leak but unaltered contractility by acute CaMKII overexpression in isolated rabbit cardiac myocytes.

    abstract::The predominant cardiac Ca2+/calmodulin-dependent protein kinase (CaMK) is CaMKIIdelta. Here we acutely overexpress CaMKIIdeltaC using adenovirus-mediated gene transfer in adult rabbit ventricular myocytes. This circumvents confounding adaptive effects in CaMKIIdeltaC transgenic mice. CaMKIIdeltaC protein expression a...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Kohlhaas M,Zhang T,Seidler T,Zibrova D,Dybkova N,Steen A,Wagner S,Chen L,Brown JH,Bers DM,Maier LS

    更新日期:2006-02-03 00:00:00

  • Role of dynamin-related protein 1 (Drp1)-mediated mitochondrial fission in oxygen sensing and constriction of the ductus arteriosus.

    abstract:RATIONALE:Closure of the ductus arteriosus (DA) is essential for the transition from fetal to neonatal patterns of circulation. Initial PO2-dependent vasoconstriction causes functional DA closure within minutes. Within days a fibrogenic, proliferative mechanism causes anatomic closure. Though modulated by endothelial-d...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Hong Z,Kutty S,Toth PT,Marsboom G,Hammel JM,Chamberlain C,Ryan JJ,Zhang HJ,Sharp WW,Morrow E,Trivedi K,Weir EK,Archer SL

    更新日期:2013-03-01 00:00:00