Comparison of morphology of bulbar conjunctival cells assessed by impression cytology versus scrape and smear methods.


PURPOSE:To assess the morphology of superficial bulbar conjunctival cells collected by impression cytology or by scraping. METHODS:Freshly procured sheep eyes from a slaughterhouse were washed and then conjunctival impression cytology (CIC) samples taken from the nasal aspect of the bulbar conjunctiva on a Biopore Millicell filter unit, or cells scraped from the same location and smeared onto glass slides. After alcohol fixation, all samples were stained with Giemsa. Cell images were obtained by light microscopy at 200× magnification, and subjected to morphometry. RESULTS:Cells obtained by scrape/smear processing appeared larger and less densely staining than cells procured by CIC. This appearance was confirmed by morphometry with measures of cell longest dimension LONG being 27.7 ± 1.3 μm and 21.7 ± 1.5 μm, respectively, while cell AREAC measures averaged 543 ± 42 μm(2) and 333 ± 51 μm(2); these differences were significant (p < 0.001). Similarly, cell nucleus length (NUCLONG) and nucleus area (AREANUC) were also larger in smeared cells (p < 0.001). Various calculations of nucleo-cytoplasmic ratios as based on dimensions or area were only slightly different when comparing cells obtained by the two methods, indicating that the glass slide-smeared cells simply spread out more than cells on the CIC filters. CONCLUSIONS:Differences in cell and nucleus size can result from use of different processing methods. Therefore, in making comparisons between sets of conjunctival cells, it is important that cell collection and processing methods be considered.


Curr Eye Res


Current eye research


Doughty MJ




Has Abstract


2014-10-01 00:00:00












  • Murine endotoxin-induced uveitis, but not immune complex-induced uveitis, is dependent on the IL-8 receptor homolog.

    abstract:PURPOSE:To determine the roles of the murine interleukin-8 receptor homolog (mIL-8Rh, neutrophil chemokine CXC receptor 2) and macrophage inflammatory protein-1alpha (MIP-1alpha, a CC chemokine) in two eye inflammation models: endotoxin-induced uveitis (EIU) and immune complex-induced uveitis (reverse passive Arthus re...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Brito BE,O'Rourke LM,Pan Y,Huang X,Park JM,Zamora D,Cook DN,Planck SR,Rosenbaum JT

    更新日期:1999-07-01 00:00:00

  • Zinc causes an apparent increase in rhodopsin phosphorylation.

    abstract:PURPOSE:Rhodopsin is a zinc-binding protein. We investigated the effect of low concentrations of zinc on the initial phosphorylation of rhodopsin. METHODS:Dark-adapted bovine rod outer segments (ROS) were incubated with (gamma 32P) ATP and 5 mM magnesium in the presence and absence of micromolar amounts of zinc. The R...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Shuster TA,Martin F,Nagy AK

    更新日期:1996-10-01 00:00:00

  • Nephropathic cystinosis: immunohistochemical and histopathologic studies of cornea, conjunctiva and iris.

    abstract::Specimens of a cornea, conjunctiva and iris were removed at the time of penetrating keratoplasty in a case of nephropathic cystinosis. Immunohistochemical studies revealed the presence of class II antigens on the stromal keratocytes of the cornea and the vascular endothelial cells, pericytes and fibroblasts of the con...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Kaiser-Kupfer MI,Chan CC,Rodrigues M,Datiles MB,Gahl WA

    更新日期:1987-04-01 00:00:00

  • A platelet-activating factor antagonist reduces corneal allograft inflammation and neovascularization.

    abstract::We assessed the role of platelet-activating factor (PAF) in corneal allograft rejection and evaluated the effects of a PAF antagonist on corneal inflammation, cellular infiltration, vascularization, and edema. Rabbits with vascularized corneas served as recipients of allogeneic cornea grafts. Rabbits with normal corne...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Cohen RA,Gebhardt BM,Bazan NG

    更新日期:1994-02-01 00:00:00

  • Multiple, spatially distinct T cell epitopes within a pathogenic 123 residue cyanogen bromide peptide of bovine retinal s-antigen.

    abstract::We have examined the T cell specificity of a Lewis rat T cell line (R208) specific for a pathogenic, 123 residue cyanogen bromide produced peptide of bovine S-antigen by using two independent sets of overlapping synthetic peptides representing the entire length of the 123 residue fragment. S-antigen, a 48 kDa immunopa...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Fling SP,Gregerson DS,Obritsch WF,Boyce-Jacino M,Merryman CF,Donoso LA

    更新日期:1990-01-01 00:00:00

  • Isotachophoresis and immunoelectrophoresis of water-soluble and -insoluble crystallins of the ageing bovine lens.

    abstract::Water-soluble (WS) and water-insoluble (WI) crystallins were prepared from calf and bovine lenses. The WS-crystallins were analyzed by capillary isotachophoresis and by immunoelectrophoresis with polyvalent and alpha-, beta-, gamma-crystallin specific antisera. The WI-crystallins were first solubilized in formamide an...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Bours J

    更新日期:1984-05-01 00:00:00

  • A Novel Approach to the Differentiation of Intrabulbar Tumors in Color Doppler Imaging.

    abstract:AIM OF THE STUDY:The similarity of many benign intrabulbar lesions to a malignant tumor requires detailed differential diagnostics. However, none of the known methods can be used as the only one to determine the type of lesions. The aim of this study was to determine color Doppler imaging (CDI) markers characteristic o...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Modrzejewska M,Wiącek MP

    更新日期:2017-11-01 00:00:00

  • Retinal oximetry using intravitreal illumination.

    abstract:PURPOSE:To demonstrate spectroscopic retinal oximetry measurements on arteries and veins in swine using intravitreal illumination. Retinal arterial and venous saturations are measured for a range of inspired O2 levels after pars plana vitrectomy. METHODS:Pars plana vitrectomy and intravitreal manipulations were perfor...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Salyer DA,Beaudry N,Basavanthappa S,Twietmeyer K,Eskandari M,Denninghoff KR,Chipman RA,Park RI

    更新日期:2006-07-01 00:00:00

  • Polarization and Distribution of Tumor-Associated Macrophages and COX-2 Expression in Basal Cell Carcinoma of the Ocular Adnexae.

    abstract:PURPOSE:Basal cell carcinoma (BCC) is a locally invasive skin tumor which can be subdivided into a circumscribed nodular and an invasive fibrosing subtype. There is increasing evidence that macrophages play an important role in interacting between tumor cells and their microenvironment, thereby affecting not only the i...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Kaiser U,Loeffler KU,Nadal J,Holz FG,Herwig-Carl MC

    更新日期:2018-09-01 00:00:00

  • PKCγ, role in lens differentiation and gap junction coupling.

    abstract:PURPOSE:To determine the role of PKCγ in the regulation of gap junction coupling in the normal lens, we have compared the properties of coupling in lenses from wild type (WT) and PKC-γ knockout (KO) mice. METHODS:Western blotting, confocal immunofluorescence microscopy, immunoprecipitation, RT-PCR and quantitative rea...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Das S,Wang H,Molina SA,Martinez-Wittinghan FJ,Jena S,Bossmann LK,Miller KA,Mathias RT,Takemoto DJ

    更新日期:2011-07-01 00:00:00

  • An in vitro model of leukocyte mediated injury to the corneal epithelium.

    abstract::To enhance efforts directed at unraveling the role and mechanisms of leukocytes in mediating injury to corneal epithelium, an isolated bovine corneal cup was developed and evaluated. Bovine peripheral leukocytes and lysates were added to the corneal epithelial surface of isolated cornea for various periods after which...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Elgebaly SA,Gillies C,Forouhar F,Hashem M,Baddour M,O'Rourke J,Kreutzer DL

    更新日期:1985-01-01 00:00:00

  • Beneficial Effects of Hypercapnic Acidosis on the Inhibition of Transforming Growth Factor β-1-induced Corneal Fibrosis in Vitro.

    abstract:PURPOSE:Corneal scarring is a common poor outcome of corneal trauma. Transforming growth factor β-1 plays a vital role in corneal fibrosis, inducing keratocyte transformation to myofibroblasts. Other than corneal transplantation, no other curative treatment methods for corneal scarring are currently available. Hypercap...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Chang YM,Cian AA,Weng TH,Liang CM,Pao SI,Chen YJ

    更新日期:2020-09-11 00:00:00

  • Liquid chromatographic determination of uric acid and ascorbic acid in rat retinae after ophthalmic artery and optic nerve ligation.

    abstract::Change of urate and ascorbate levels in retinae of rat left eyes after ophthalmic artery and optic nerve ligation was studied by reversed-phase high-performance liquid chromatography with electrochemical detection. The right eyes were used for sham operation. We found that uric acid levels in the retinae of the left e...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Iriyama K,Yoshiura M,Iwamoto T,Kamada Y,Nozawa H,Mizuno A,Matsuzaki H

    更新日期:1986-07-01 00:00:00

  • 24-hour intraocular pressure and blood pressure levels with latanoprost/timolol fixed combination versus timolol.

    abstract:PURPOSE:To evaluate 24-hr intraocular pressure (IOP) and blood pressure (BP) with timolol or latanoprost/timolol fixed combination (LTFC). METHODS:Patients with primary open-angle glaucoma or ocular hypertension with normal blood pressure were randomized to LTFC, dosed each evening, or timolol dosed twice daily in a c...

    journal_title:Current eye research

    pub_type: 杂志文章,随机对照试验


    authors: Konstas AG,Pikilidou MI,Tsironi S,Mikropoulos D,Kozobolis VP,Sarafidis PA,Lasaridis AN,Nelson LA,Stewart WC

    更新日期:2009-05-01 00:00:00

  • Neutralizing antibody to TGFbeta modulates stromal fibrosis but not regression of photoablative effect following PRK.

    abstract:PURPOSE:Previous studies have suggested that corneal fibrosis controlled by a TGFbeta-mediated cytocrine pathway underlies the development of clinical corneal haze and associated regression of photoablative effect following excimer laser PRK. Using a unique blocking antibody, we evaluated the role of TGFbeta in post-PR...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Møller-Pedersen T,Cavanagh HD,Petroll WM,Jester JV

    更新日期:1998-07-01 00:00:00

  • Effect of intraocular irrigating solutions on the viability of cultured retinal vascular endothelial cells.

    abstract:PURPOSE:To compare the abilities of balanced salt solution, BSS Plus and Hartmann's lactated Ringer's (HLR) solution to maintain the viability of retinal vascular endothelial cells (RVEC) in vitro. METHODS:Cultured retinal vascular endothelial cells were suspended in each irrigating solution for four hours. Viability ...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Mourad KM,Edelhauser HF,Capone A Jr,Lynn MJ,Geroski DH

    更新日期:1997-03-01 00:00:00

  • Dry Eye-Related Visual Blurring and Irritative Symptoms and Their Association with Depression and Anxiety in Eye Clinic Patients.

    abstract:PURPOSE:Dry eye is a common condition known to have many systemic co-morbidities. We aim to report the frequency of depression and anxiety suspects in eye clinic patients and explore the association between dry eye signs and symptoms with depression and anxiety. MATERIALS AND METHODS:Adult outpatients were recruited f...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Liyue H,Chiang PP,Sung SC,Tong L

    更新日期:2016-05-01 00:00:00

  • Intracorneal lesions produced with focused ultrasound.

    abstract::Focused ultrasound concentrates a large amount of ultrasonic energy into a small focal zone. We have used new techniques to develop a transducer that creates intracorneal, thermally induced lesions in rabbit eyes in vitro. Histologic study shows that these lesions are confined to the stroma and that the procedure caus...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Mark DB,Beuerman R

    更新日期:1982-01-01 00:00:00

  • Open-angle Glaucoma and Sensorineural Hearing Impairment in the Korean Population.

    abstract:PURPOSE:This study identifies the prevalence and risk factors of sensorineural hearing loss and primary open-angle glaucoma (POAG). MATERIALS AND METHODS:Patients aged 19 years or older who had undergone both ophthalmologic examination and audiometry as part of the Korea National Health and Nutrition Examination Surve...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Kim JM,Lee MY,Kim JW,Lee J,Kim HJ,Cha SC,Kim NR

    更新日期:2020-08-01 00:00:00

  • Effect of repeated applanation tonometry on the accuracy of intraocular pressure measurements.

    abstract:PURPOSE:To evaluate the stability of successive applanation tonometry measurements in glaucomatous eyes. METHODS:A prospective, comparative, randomized, and evaluator-masked study was conducted in a tertiary medical center. Sixty-seven patients with glaucoma attending our glaucoma clinic underwent four successive intr...

    journal_title:Current eye research

    pub_type: 杂志文章,随机对照试验


    authors: Gaton DD,Ehrenberg M,Lusky M,Wussuki-Lior O,Dotan G,Weinberger D,Snir M

    更新日期:2010-06-01 00:00:00

  • Phosphorylation of chick lens proteins.

    abstract::Phosphorylated proteins of the chick lens were identified following incubation of lenses in a medium containing 32P and subsequent analysis by gel electrophoresis. The acidic variant of the vimentin and both subunits of fodrin were phosphorylated, as were the 95 Kd and 49 Kd proteins associated with the beaded-chain f...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Ireland M,Maisel H

    更新日期:1984-07-01 00:00:00

  • Controlled modulation of the phase separation and opacification temperature of purified bovine gamma IV-crystallin.

    abstract::In the bovine lens the gamma IV-crystallin fraction is a principal determinant of the phase separation and opacification temperature, Tc (Siezen et al, Proc. Natl. Acad. Sci. USA 82, 1985, 1701). We have now measured the effect on Tc of purified gamma IV-crystallin solutions produced by a variety of reagents which aff...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Siezen RJ,Benedek GB

    更新日期:1985-10-01 00:00:00

  • Safety and efficacy of loteprednol etabonate for treatment of papillae in contact lens-associated giant papillary conjunctivitis.

    abstract::Loteprednol etabonate (LE) is a new corticosteroid based on the "soft drug" concept. Contact lens-associated giant papillary conjunctivitis (GPC) was studied as a model for the anti-inflammatory effect of LE. Patients with bilateral GPC were enrolled in a multicenter, randomized, double-masked, placebo-controlled, par...

    journal_title:Current eye research

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Bartlett JD,Howes JF,Ghormley NR,Amos JF,Laibovitz R,Horwitz B

    更新日期:1993-04-01 00:00:00

  • Interleukin-6 and Diabetic Retinopathy: A Systematic Review and Meta-Analysis.

    abstract:BACKGROUND:Interleukin-6 (IL-6) is produced by a variety of cells involved in inflammation and acts as local intensification signals in pathological processes associated with chronic eye inflammation. This meta-analysis was performed to provide a better understanding of the relationship between IL-6 and diabetic retino...

    journal_title:Current eye research

    pub_type: 杂志文章,meta分析


    authors: Yao Y,Li R,Du J,Long L,Li X,Luo N

    更新日期:2019-05-01 00:00:00

  • The effect of short-term hypobaric hypoxic exposure on intraocular pressure.

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    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Karadag R,Sen A,Golemez H,Basmak H,Yildirim N,Karadurmus N,Koseoglu E,Akin A

    更新日期:2008-10-01 00:00:00

  • Comparison of Short-Term Choroidal Thickness and Retinal Morphological Changes after Intravitreal Anti-VEGF Therapy with Ranibizumab or Aflibercept in Treatment-Naive Eyes.

    abstract:PURPOSE:To evaluate choroidal thickness (CT) and retinal morphological changes in eyes with neovascular age-related macular degeneration (nAMD) following ranibizumab or aflibercept intravitreal treatment. MATERIALS AND METHODS:This was a prospective, observational, comparative study where 76 eyes of 76 consecutive pat...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Gharbiya M,Giustolisi R,Marchiori J,Bruscolini A,Mallone F,Fameli V,Nebbioso M,Abdolrahimzadeh S

    更新日期:2018-03-01 00:00:00

  • Relationship between retinal fractal dimensions and retinal circulation in patients with type 2 diabetes mellitus.

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    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Nagaoka T,Yoshida A

    更新日期:2013-11-01 00:00:00

  • Rose bengal induced corneal swelling: relation to inciting wavelength.

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    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Hull DS,Csukas S,Green K

    更新日期:1981-01-01 00:00:00

  • Effect of leukopenia on experimental post-traumatic retinal detachment.

    abstract::Macrophages are invariably present in the intraocular membranes of patients with traumatic proliferative vitreoretinopathy (PVR). There are two sources from which these macrophages could be recruited: adjacent tissues and the systemic circulation. In the study described herein, the role of circulating white blood cell...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Gabrielian K,Wang HM,Lee M,Ogden TE,Ryan SJ

    更新日期:1994-01-01 00:00:00

  • Growth curve for Propionibacterium acnes.

    abstract::We established growth curves for Propionibacterium acnes isolates recovered from eyes with chronic postoperative endophthalmitis. The growth curve plotted the average of the duplicate bacterial concentration against time. The generation time for P. acnes calculated from the growth curves was approximately 5.1 hours. T...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Hall GS,Pratt-Rippin K,Meisler DM,Washington JA,Roussel TJ,Miller D

    更新日期:1994-06-01 00:00:00