Anticoagulant and antithrombotic actions of a semisynthetic beta-1,3-glucan sulfate.


:Sulfation of the natural polysaccharide curdlan results in anticoagulantly active beta-1,3-glucan sulfates whose activity depends on various structural parameters. In this study the anticoagulant and antithrombotic effects of one of these beta-1,3-glucan sulfates (GS) were compared with those of a porcine mucosal heparin. GS produced a concentration dependent anticoagulant effect in all the global coagulation assays with the exception of the anti-Xa assay. The best activity was found in the APTT and the thrombin time assays indicating that protease generation and the direct inhibition of thrombin may be sites of actions of this agent. Whereas the anticoagulant activity of GS was approximately 5 fold lower compared to heparin, a 32 fold higher concentration (ED50 = 550 micrograms/kg) was needed for an antithrombotic effect similar to heparin (ED50 = 17.2 micrograms/kg) in a rabbit model of stasis thrombosis. In contrast to this, when a rat model of clamping induced jugular vein occlusion was used to produce vascular obstruction, GS produced similar antithrombotic actions to heparin. At a 250 micrograms/kg dosage, both agents doubled the number of clampings required for complete vascular obstruction. Since the mechanical injury to the blood vessel is the primary determinant of the thrombogenic response, GS may inhibit some of the pathophysiologic mechanisms responsible for the occlusion of the blood vessel. The current study also points to the fact that the global anticoagulant effects may not reflect the antithrombotic potential of newer sulfated carbohydrate derived drugs.


Thromb Res


Thrombosis research


Alban S,Jeske W,Welzel D,Franz G,Fareed J




Has Abstract


1995-05-01 00:00:00














  • Effect of thromboxane synthase inhibition on the thrombolytic action of tissue-type plasminogen activator in a rabbit model of peripheral arterial thrombosis.

    abstract::The thrombolytic efficacy of recombinant tissue-type plasminogen activator (tPA) in the presence and absence of a thromboxane synthase inhibitor was studied in a model of femoral artery thrombosis in the anesthetized rabbit. The thrombus was formed by injection of thrombin and whole blood into an isolated segment of t...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Shebuski RJ,Storer BL,Fujita T

    更新日期:1988-12-01 00:00:00

  • PROPLEX vs. PROPLEX SX: a controlled double blind study of the effectiveness in treating acute hemarthroses in hemophilia A patients with inhibitors to factor VIII.

    abstract::A blinded randomized multicenter trial of two non-activated prothrombin complex concentrates was carried out to determine the clinical effectivity in the treatment of acute hemarthrosis in hemophiliac patients with inhibitors. The one product was prepared via DEAE Sephadex chromatography, while the second was fraction...

    journal_title:Thrombosis research

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Gomperts ED,Fannon RB,Lee ML,Kingdon HS,Kantrowitz JL,Addiego JE Jr,Carney J,Corrigan JJ,Hathaway W,Hilgartner MW

    更新日期:1986-06-15 00:00:00

  • Correlation of coagulation markers and 4F-PCC-mediated reversal of rivaroxaban in a rabbit model of acute bleeding.

    abstract:INTRODUCTION:Rivaroxaban is an oral, selective direct factor Xa inhibitor approved for several indications in patients at risk of thrombotic events. One limitation of its clinical use is the lack of data pertaining to its reversal in situations where urgent response is critical (e.g. acute bleeding events or emergency ...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Herzog E,Kaspereit F,Krege W,Mueller-Cohrs J,Doerr B,Niebl P,Dickneite G

    更新日期:2015-03-01 00:00:00

  • Normal range of mean platelet volume in healthy subjects: Insight from a large epidemiologic study.

    abstract:AIM:Mean platelet volume (MPV) in the healthy population has not been studied before. Therefore, the aim of the study was to measure MPV in normal subjects in a large cohort of Turkish adults. METHODS:A total of 2298 subjects with a mean age of 50 (age range 18 to 92) were interviewed. Subjects who had smoking habit, ...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Demirin H,Ozhan H,Ucgun T,Celer A,Bulur S,Cil H,Gunes C,Yildirim HA

    更新日期:2011-10-01 00:00:00

  • The anti-thrombotic effect of hydrogen sulfide is partly mediated by an upregulation of nitric oxide synthases.

    abstract:INTRODUCTION:Hydrogen sulfide (H2S) known as a gasotransmitter is increasingly recognized for its anti-adhesive, anti-inflammatory and vasoactive properties. Due to these properties, we analysed anti-thrombotic effects of H2S and the participation of the nitric oxide synthase (NOS)-pathway. MATERIALS AND METHODS:In in...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Kram L,Grambow E,Mueller-Graf F,Sorg H,Vollmar B

    更新日期:2013-08-01 00:00:00

  • Thrombin enhances tumor cell adhesive and metastatic properties via increased alpha IIb beta 3 expression on the cell surface.

    abstract::The association between blood coagulation and cancer growth and metastatic dissemination is not yet completely understood. In this study we demonstrate that thrombin is capable of enhancing tumor cell adhesive properties and thereby increases tumor cell metastatic potential. Following exposure to alpha-thrombin, Walke...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Wojtukiewicz MZ,Tang DG,Nelson KK,Walz DA,Diglio CA,Honn KV

    更新日期:1992-11-01 00:00:00

  • A genome-wide search for genes affecting circulating fibrinogen levels in the Framingham Heart Study.

    abstract:INTRODUCTION:Circulating levels of fibrinogen are associated with atherosclerosis and predict future coronary heart disease and stroke. Levels of fibrinogen are correlated among family members, suggesting a heritable component. Variants of the beta-fibrinogen gene subunit on 4q28 are associated with fibrinogen levels b...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Yang Q,Tofler GH,Cupples LA,Larson MG,Feng D,Lindpaintner K,Levy D,D'Agostino RB,O'Donnell CJ

    更新日期:2003-04-15 00:00:00

  • Specific binding of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor) to the intact canine platelet.

    abstract::Binding of 3H-labeled 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor; PAF) to the intact, washed canine platelet has been defined and characterized as being specific and receptor-mediated. Under the conditions described, specific binding to 2 X 10(7) canine platelets reached saturation with...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Janero DR,Burghardt B,Burghardt C

    更新日期:1988-06-15 00:00:00

  • Effectiveness and safety of different duration of thromboprophylaxis in 16,865 hip replacement patients--a real-word, prospective observational study.

    abstract:INTRODUCTION:Clinical trials have provided evidence about efficacy and safety of extended thromboprophylaxis among total hip replacement (THR) patients. There is a lack of evidence on effectiveness and safety of extended treatment in unselected patients from routine clinical practice. We examined the effectiveness and ...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Pedersen AB,Sorensen HT,Mehnert F,Johnsen SP,Overgaard S

    更新日期:2015-02-01 00:00:00

  • MiR-188-3p upregulation results in the inhibition of macrophage proinflammatory activities and atherosclerosis in ApoE-deficient mice.

    abstract:BACKGROUND:Atherosclerosis occurs as a result of a chronic inflammatory response in the arterial wall associated with an increased uptake of low-density lipoprotein by macrophages and the subsequent transformation of this lipoprotein into foam cells. It has been found that miR-188-3p can suppress autophagy and myocardi...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Zhang XF,Yang Y,Yang XY,Tong Q

    更新日期:2018-11-01 00:00:00

  • G alpha q cDNA sequence from human platelets.

    abstract::G-proteins play a major role in cell signaling and specific abnormalities in these proteins have been implicated in some diseases. Although, two human G alpha q coding sequences are published, they differ at functionally important regions. We have sequenced the human G alpha q cDNA synthesized from normal platelet mRN...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Gabbeta J,Dhanasekaran N,Rao AK

    更新日期:1998-07-01 00:00:00

  • Protein C and protein S changes in GH-deficient adults on r-HGH replacement therapy: correlations with PAI-1 and t-PA plasma levels.

    abstract:BACKGROUND:In the rat liver, growth hormone (GH) affects the synthesis of vitamin-K-dependent factors, including Protein C (prot.C) and protein S (prot.S), two natural anticoagulants that prevent hypercoagulable states. Adults with GH deficiency (GHD) are at risk of thrombotic events. High circulating levels of PAI-1 a...

    journal_title:Thrombosis research

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Di Minno MN,Pezzullo S,Palmieri V,Di Somma C,Lupoli R,Valle D,Lombardi G,Di Minno G

    更新日期:2010-12-01 00:00:00

  • Correlation between cardiac biomarkers and right ventricular enlargement on chest CT in non massive pulmonary embolism.

    abstract:BACKGROUND:Troponin I (cTnI), myoglobin, heart-type fatty acid binding protein (H-FABP), and natriuretic peptides (BNP, NTproBNP) were all reported to be elevated in patients with pulmonary embolism (PE). METHODS:To assess the correlation between the aforementioned markers and helical computed tomography (hCT) right v...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Vuilleumier N,Righini M,Perrier A,Rosset A,Turck N,Sanchez JC,Bounameaux H,Le Gal G,Mensi N,Hochstrasser D

    更新日期:2008-01-01 00:00:00

  • Management of heparin-induced thrombocytopenia: a critical comparison of lepirudin and argatroban.

    abstract::Heparin-induced thrombocytopenia (HIT) is a transient hypercoagulability state initiated, paradoxically, by the anticoagulant, heparin. It is characterized by antibody-induced activation of platelets, leading to thrombin generation. Many patients with HIT develop thrombosis; even when heparin is stopped because of "is...

    journal_title:Thrombosis research

    pub_type: 杂志文章,评审


    authors: Warkentin TE

    更新日期:2003-05-01 00:00:00

  • Thromboembolic complications several days after a single-dose administration of aspirin.

    abstract::The antithrombotic properties of acetyl salicylic acid (ASA) used at current doses are largely demonstrated. However, our previous study showed unexpected thrombotic potencies associated with the use of this drug. In this study we investigate the effect of aspirin on an experimental thrombosis induced by laser beams, ...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Aguejouf O,Belougne-Malfatti E,Doutremepuich F,Belon P,Doutremepuich C

    更新日期:1998-02-01 00:00:00

  • A novel approach to thrombin inhibition.

    abstract::The inability of the heparin-antithrombin complex to inhibit fibrin-bound thrombin limits the utility of heparin for treatment of arterial thrombosis. In contrast with heparin, melagatran, a direct thrombin inhibitor (DTI), is equally effective at inhibiting fluid-phase thrombin and thrombin bound to fibrin. This refl...

    journal_title:Thrombosis research

    pub_type: 杂志文章,评审


    authors: Weitz JI

    更新日期:2003-07-15 00:00:00

  • Assessing the clinical and cost impact of on-demand immunoassay testing for the diagnosis of heparin induced thrombocytopenia.

    abstract:BACKGROUND:The diagnostic work-up for heparin induced thrombocytopenia (HIT) can take several days. Consequently patients may be speculatively switched onto replacement anticoagulant therapy before a diagnosis is confirmed. On-demand immunoassay diagnostic testing enables timely treatment decisions, based on test resul...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Caton S,O'Brien E,Pannelay AJ,Cook RG

    更新日期:2016-04-01 00:00:00

  • Outpatient management of pulmonary embolism.

    abstract::Despite clear potential benefits of outpatient care, most patients suffering from pulmonary embolism (PE) are currently hospitalized due to the fear of possible adverse events. Nevertheless, some teams have increased or envisage to increase outpatient treatment or early discharge. We performed a narrative systematic r...

    journal_title:Thrombosis research

    pub_type: 杂志文章,评审


    authors: Roy PM,Moumneh T,Penaloza A,Sanchez O

    更新日期:2017-07-01 00:00:00

  • The action of thrombin on peptide p-nitroanilide substrates: hydrolysis of Tos-Gly-Pro-Arg-pNA and D-Phe-Pip-Arg-pNA by human alpha and gamma and bovine alpha and beta-thrombins.

    abstract::Human and bovine alpha-thrombins (greater than 90% alpha form) with high fibrinogen clotting activities (approximately 3,000 U.S. units/mg protein) exhibit similar Michaelis menten kinetics with the p-nitroanilide tripeptide substrates Tos-Gly-Pro-arg-pNA (Chromozym-TH) and D-Phe-Pip-Arg-pNA (S-2238). The kinetic para...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Lottenberg R,Hall JA,Fenton JW 2nd,Jackson CM

    更新日期:1982-11-01 00:00:00

  • Efficacy and safety of the use of heparin as thromboprophylaxis in patients with liver cirrhosis: a systematic review and meta-analysis.

    abstract:INTRODUCTION:Venous thromboembolism is a common cause of morbidity and mortality. Although cirrhosis has classically been considered as an acquired bleeding diathesis, there is increasing evidence that rejects the traditional belief that these patients are naturally protected against venous thromboembolism. However, an...

    journal_title:Thrombosis research

    pub_type: 杂志文章,meta分析,评审


    authors: Gómez Cuervo C,Bisbal Pardo O,Pérez-Jacoiste Asín MA

    更新日期:2013-10-01 00:00:00

  • Noninvasive transcutaneous ultrasound augments thrombolysis in the left circumflex coronary artery--an in vivo canine study.

    abstract:BACKGROUND:Ultrasound has the potential to augment chemical thrombolysis. METHODS AND RESULTS:Thrombotic occlusions in the left circumflex artery (LCx) were induced in 27 dogs. Sixty minutes later, tissue-type plasminogen activator (t-PA) was given intravenously over 90 min. Thrombotic occlusions (n = 20) were treated...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Jeon DS,Luo H,Fishbein MC,Miyamoto T,Horzewski M,Iwami T,Mirocha JM,Ikeno F,Honda Y,Siegel RJ

    更新日期:2003-05-01 00:00:00

  • Application of an enzyme-linked immunosorbent assay (ELISA) to von Willebrand factor (vWF) and its derivatives.

    abstract::Sandwich ELISA systems were developed to measure human von Willebrand factor (vWF). In one system the microtitre plates were coated with goat F(ab')2 to vWF. The F(ab')2 different molecular forms in the soluble phase. VWF antigen bound by the F(ab')2 antibody was subsequently determined by using horse-radish peroxidas...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Silveira AM,Yamamoto T,Adamson L,Hessel B,Blombäck B

    更新日期:1986-07-01 00:00:00

  • Aprotinin inhibits urokinase but not tissue-type plasminogen activator.

    abstract::The protease inhibitor, aprotinin, has been examined for its ability to inhibit urokinase and tissue-type plasminogen activators at pH 7.4 in assays utilizing pyroGlu-Gly-Arg-p-nitroanilide and H-D-Ile-Pro-Arg-p-nitroanilide as substrates, respectively. Aprotinin inhibited both two-chain low molecular weight urokinase...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Lottenberg R,Sjak-Shie N,Fazleabas AT,Roberts RM

    更新日期:1988-03-15 00:00:00

  • Effects of exercise test on plasma markers of an activation of coagulation and/or fibrinolysis in patients with symptomatic or silent myocardial ischemia.

    abstract::It has been suggested that unstable angina at rest, like acute myocardial infarction, might be associated with a thrombotic process. In order to study the hypothesis that myocardial ischemia during exercise could also be associated with an activation of blood coagulation and/or fibrinolysis, we investigated the presen...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Bounameaux H,Righetti A,de Moerloose P,Bongard O,Reber G

    更新日期:1992-01-01 00:00:00

  • Effects of heparin and a semi-synthetic heparin analogue on platelet aggregation, lipoprotein lipase and other laboratory tests in surgical patients.

    abstract::Platelet aggregation, lipoprotein lipase activity, coagulation parameters and routine blood chemistry were measured in a randomised study of 21 surgical patients before, immediately after and 3 months after operation. Sodium heparin 5000 IU was given subcutaneously to 11 patients every 12 hours for 7 days, the first i...

    journal_title:Thrombosis research

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Törngren S,Norén I,Forsskåhl B,Sipilä H

    更新日期:1983-06-01 00:00:00

  • Symptomatic and asymptomatic deep vein thrombosis after total hip replacement. Differences in phlebographic pattern, described by a scoring of the thrombotic burden.

    abstract::The aim was to describe the phlebographic pattern of asymptomatic and symptomatic deep vein thrombosis (DVT) after total hip replacement by the use of a scoring system in 102 consecutive patients (54 asymptomatic, 48 symptomatic). The DVTs were scored from 1 to 3, and registered in a scoring system dividing the deep v...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Björgell O,Nilsson PE,Benoni G,Bergqvist D

    更新日期:2000-09-01 00:00:00

  • Fresh frozen plasma in the pediatric age group and in congenital coagulation factor deficiency.

    abstract::Generally, the rules of good practice in transfusion medicine apply also to the pediatric age group. However, the frequency of specific diseases that might necessitate the administration of fresh frozen plasma (FFP) differs from that in adults. Physiologic differences to the later age exist in the neonatal period and ...

    journal_title:Thrombosis research

    pub_type: 杂志文章,评审


    authors: Muntean W

    更新日期:2002-10-31 00:00:00

  • Thrombin generation in patients undergoing carotid endarterectomy: implications in acute vessel wall closure and antithrombotic therapy.

    abstract::We determined the extent and duration of activation of coagulation during the first 24 hours after carotid endarterectomy. Serial blood samples were collected before, during and after surgery from 10 patients with severe stenosis (> 70%) undergoing carotid endarterectomy with heparinization. Platelet poor plasmas prep...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Wells J,Nicosia S,Wale C,Smith LJ,Buchanan MR

    更新日期:1994-08-15 00:00:00

  • Platelet and neutrophil function and eicosanoid release in a subject with abetalipoproteinaemia.

    abstract::It has been suggested that plasma low density lipoprotein (LDL) may affect platelet and leukocyte activity and the release of biologically active eicosanoids which may contribute to the pathogenesis of coronary heart disease. To evaluate the possible biological effects of LDL further we have studied platelet aggregati...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Croft KD,Beilin LJ

    更新日期:1993-02-15 00:00:00

  • Changes of the fibrinolytic system of animals induced by injection of tissue plasminogen activator.

    abstract::The changes in the fibrinolytic system of rats induced by repeated injections of tissue plasminogen activator (TPA) have been studied. The euglobulin fraction fibrinolytic activity increases 10 min after the first injection of TPA. Repeated injections of TPA for one or more days do not stimulate the fibrinolytic activ...

    journal_title:Thrombosis research

    pub_type: 杂志文章


    authors: Andreenko GV,Lyutova LV,Shimonaeva EE

    更新日期:1982-08-01 00:00:00