mTHPC Polymer Conjugates: The In Vivo Photodynamic Activity of Four Candidate Compounds.


:The in vivo photodynamic activities of four poly(ethylene glycol) (PEG) conjugates of the photosensitiser 5,10,15,20-tetrakis-(m-hydroxyphenyl)chlorin (mTHPC, temoporfin, Foscan(®)) were compared with that of mTHPC over a range of drug-light intervals using acute tumour necrosis and skeletal muscles swelling in a mouse model in order to ascertain the influence of linking group stability and PEG chain length on the photodynamic activity. The four compounds examined contained either PEG 2000 or PEG 5000 attached by carbonate or triazine linkages at the phenol hydroxyl groups of the mTHPC.All compounds tested caused tumour necrosis at drug-light intervals of between one and four days. mTHPC produced tumour necrosis of over 5 mm at drug-light intervals of 1 and 2 days with limited muscle damage at early drug-light intervals. The relatively labile carbonate-linked conjugates gave tumour necrosis similar to mTHPC but produced severe muscle and systemic phototoxicity on irradiation at 4-24 h after injection. The more stable triazine-linked conjugates produced no significant muscle damage at any of the drug-light intervals tested, but gave only limited tumour necrosis under the conditions tested. PEG chain length had relatively little effect on the patterns of bioactivity.It is concluded that both classes of mTHPC PEG conjugates may be suitable for photodynamic therapy if the problems of stability and early photosensitivity in the case of the carbonates and reduced potency in the case of the triazines can be overcome through improved formulations and PDT treatment regimens.


Lasers Med Sci


Grahn MF,Giger A,McGuinness A,de Jode ML,Stewart JC,Ris HB,Altermatt HJ,Williams NS




Has Abstract


1999-03-01 00:00:00












  • High-frequency low-level diode laser irradiation promotes proliferation and migration of primary cultured human gingival epithelial cells.

    abstract::In periodontal therapy, the use of low-level diode lasers has recently been considered to improve wound healing of the gingival tissue. However, its effects on human gingival epithelial cells (HGECs) remain unknown. The aim of the present study was to examine whether high-frequency low-level diode laser irradiation st...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Ejiri K,Aoki A,Yamaguchi Y,Ohshima M,Izumi Y

    更新日期:2014-07-01 00:00:00

  • Atomic force microscopy investigation of the interaction of low-level laser irradiation of collagen thin films in correlation with fibroblast response.

    abstract::Low-level red laser (LLRL)-tissue interactions have a wide range of medical applications and are garnering increased attention. Although the positive effects of low-level laser therapy (LLLT) have frequently been reported and enhanced collagen accumulation has been identified as one of the most important mechanisms in...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Stylianou A,Yova D

    更新日期:2015-12-01 00:00:00

  • Effect of light polarization on the efficiency of photodynamic therapy of basal cell carcinomas: an in vitro cellular study.

    abstract::In an in vitro study, the effect of light polarization on the efficiency of 5-aminolaevulinic acid (ALA) photodynamic therapy (PDT) of basal cell carcinoma (BCC) was investigated. Three states of light polarization (non-polarized, linearly polarized, and circularly polarized) were considered. Cells were exposed to gre...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: JalalKamali M,Nematollahi-Mahani SN,Shojaei M,Shamsoddini A,Arabpour N

    更新日期:2018-02-01 00:00:00

  • Antiinflammatory effect of low-level laser therapy on Staphylococcus epidermidis endophthalmitis in rabbits.

    abstract::A rabbit model of endophthalmitis was established to evaluate the antiinflammatory effect of low-level laser therapy (LLLT) as an adjunct to treatment for Staphylococcus epidermidis endophthalmitis. Rabbits were randomly divided into three groups to receive intravitreal injections into their left eye: group A received...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Ma WJ,Li XR,Li YX,Xue ZX,Yin HJ,Ma H

    更新日期:2012-05-01 00:00:00

  • Effect of laser preparation on bond strength of a self-adhesive flowable resin.

    abstract::The aim of this in vitro study was to evaluate the effect of laser treatment on shear bond strength of a self-adhesive flowable resin composite to human dentin. Eighty extracted sound human molar teeth were used for the study. The teeth were sectioned mesiodistally and embedded in acrylic blocks. The dentin surfaces w...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Yazici AR,Agarwal I,Campillo-Funollet M,Munoz-Viveros C,Antonson SA,Antonson DE,Mang T

    更新日期:2013-01-01 00:00:00

  • Effect of Er:YAG laser application on the shear bond strength and microleakage between resin cements and Y-TZP ceramics.

    abstract::The objective of this study was to investigate the effect of Er:YAG laser irradiation on shear bond strength and microleakage between resin cements and yttrium-stabilized tetragonal zirconia (Y-TZP) ceramics. Eighty disc specimens of Y-TZP ceramics (6 mm × 4 mm) were prepared. The specimens were divided into two group...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Akin H,Tugut F,Akin GE,Guney U,Mutaf B

    更新日期:2012-03-01 00:00:00

  • Efficacy of high and low level laser therapy in the treatment of Bell's palsy: a randomized double blind placebo-controlled trial.

    abstract::The aim of the present study was to investigate and compare the effects of high intensity laser therapy (HILT) and low level laser therapy (LLLT) on the treatment of patients with Bell's palsy. Forty-eight patients participated in and completed this study. The mean age was 43 ± 9.8 years. They were randomly assigned i...

    journal_title:Lasers in medical science

    pub_type: 杂志文章,随机对照试验


    authors: Alayat MS,Elsodany AM,El Fiky AA

    更新日期:2014-01-01 00:00:00

  • Prolonged post-stimulation response induced by 980-nm infrared neural stimulation in the rat primary motor cortex.

    abstract::The post-stimulation response of neural activities plays an important role to evaluate the effectiveness and safety of neural modulation techniques. Previous studies have established the capability of infrared neural modulation (INM) on neural firing regulation in the central nervous system (CNS); however, the dynamic...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Wang M,Xia Q,Peng F,Jiang B,Chen L,Wu X,Zheng X,Wang X,Tian T,Hou W

    更新日期:2020-03-01 00:00:00

  • Efficacy and outcome of holmium laser enucleation of prostate in patients with urinary retention due to advanced prostate cancer.

    abstract::To evaluate the efficacy and safety of holmium laser enucleation of prostate (HoLEP) for treating urinary retention in patients with advanced prostate cancer. Thirty-eight cases of advanced prostate cancer with urinary retention were enrolled in this retrospective study. All the 38 patients were treated with CAB as a ...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Tang M,Wang C,Li P,Zhang Q,Qian J,Meng X

    更新日期:2020-08-01 00:00:00

  • Photobiomodulation invigorating collagen deposition, proliferating cell nuclear antigen and Ki67 expression during dermal wound repair in mice.

    abstract::The present investigation focuses on understanding the role of photobiomodulation in enhancing tissue proliferation. Circular excision wounds of diameter 1.5 cm were created on Swiss albino mice and treated immediately with 2 J/cm2 and 10 J/cm2 single exposures of the Helium-Neon laser along with sham-irradiated contr...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Prabhu V,Rao BSS,Rao ACK,Prasad K,Mahato KK

    更新日期:2020-11-27 00:00:00

  • Influence of different laser-assisted retrograde cavity preparation techniques on bond strength of bioceramic-based material to root dentine.

    abstract::The purposes of the study were to evaluate the bond strength of bioceramic TotalFill root repair material (RRM) in retrograde cavities prepared using Er:YAG and Er,Cr:YSGG laser and steel bur, and to analyze failure modes. The root canals of 30 single-rooted teeth were endodontically treated, their root-ends were rese...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Kadić S,Baraba A,Miletić I,Ionescu AC,Brambilla E,Ivanišević Malčić A,Gabrić D

    更新日期:2020-02-01 00:00:00

  • Comparison of microleakages of photo-cured composites using three different light sources: halogen lamp, LED and argon laser: an in vitro study.

    abstract::In this study, we compared the microleakage of composite fillings cured with halogen bulb, LED and argon ion laser (488 nm). Twenty-four extracted human molars were divided randomly in three groups. Six cavities were prepared on the coronal part of each tooth. Standard cavities (1.7 x 2 mm) were prepared. Cavities wer...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Tielemans M,Compere P,Geerts SO,Lamy M,Limme M,De Moor RJ,Delmé KI,Bertrand MF,Rompen E,Nammour S

    更新日期:2009-01-01 00:00:00

  • Low-level laser irradiation, cyclooxygenase-2 (COX-2) expression and necrosis of random skin flaps in rats.

    abstract::Skin flaps are still a matter of concern among surgeons, as failures can occur leading to flap necrosis. However, low-level laser irradiation has been reported as an effective tool to improve the viability of ischemic flaps, yet its mechanisms of action remain unclear. We investigated the effect of low-level laser irr...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Esteves Junior I,Masson IB,Oshima CT,Paiotti AP,Liebano RE,Plapler H

    更新日期:2012-05-01 00:00:00

  • Relevance of laser irradiance threshold in the induction of alkaline phosphatase in human osteoblast cultures.

    abstract::Induction of matrix synthesis by low-level laser has been demonstrated extensively. However, the question of dose- or power intensity-dependency is under-investigated. To address this issue we chose human osteoblast cell cultures and measured their alkaline phosphatase (ALP) activity after laser irradiation. The cell ...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Haxsen V,Schikora D,Sommer U,Remppis A,Greten J,Kasperk C

    更新日期:2008-10-01 00:00:00

  • Effects of pre-irradiation of low-level laser therapy with different doses and wavelengths in skeletal muscle performance, fatigue, and skeletal muscle damage induced by tetanic contractions in rats.

    abstract::This study aimed to evaluate the effects of low-level laser therapy (LLLT) immediately before tetanic contractions in skeletal muscle fatigue development and possible tissue damage. Male Wistar rats were divided into two control groups and nine active LLLT groups receiving one of three different laser doses (1, 3, and...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Santos LA,Marcos RL,Tomazoni SS,Vanin AA,Antonialli FC,Grandinetti Vdos S,Albuquerque-Pontes GM,de Paiva PR,Lopes-Martins RÁ,de Carvalho Pde T,Bjordal JM,Leal-Junior EC

    更新日期:2014-09-01 00:00:00

  • Low-level 809 nm GaAlAs laser irradiation increases the proliferation rate of human laryngeal carcinoma cells in vitro.

    abstract::The aim of the study was to investigate the effect of low-level 809 nm laser irradiation on the proliferation rate of human larynx carcinoma cells in vitro. Epithelial tumor cells were obtained from a laryngeal carcinoma and cultured under standard conditions. For laser treatment the cells were spread on 96-well tissu...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Kreisler M,Christoffers AB,Willershausen B,d'Hoedt B

    更新日期:2003-01-01 00:00:00

  • Effect of diode laser application as an adjunct to nonsurgical periodontal therapy on the reduction of red complex microorganisms in type 2 diabetics with chronic periodontitis.

    abstract::Bactericidal and detoxification effects of diode laser (DL) have been reported in periodontal treatment. The objective of this study was investigating the additional effect of DL with nonsurgical periodontal treatment on the red complex bacteria in type 2 diabetes mellitus (DM) patients with chronic periodontitis (CP)...

    journal_title:Lasers in medical science

    pub_type: 杂志文章,随机对照试验


    authors: Kocak E,Sağlam M,Arslan U,Kayis SA,Kebapcilar L,Loos BG,Hakki SS

    更新日期:2020-08-01 00:00:00

  • Construction of a novel port wine stain phantom and measurement of colour by digital imaging and reflectance spectrophotometry.

    abstract::A novel skin phantom is described that is constructed with quantifiable amounts of skin pigments, as well as a light scattering medium in the form of barium sulphate suspension. The two predominant skin pigments (melanin and haemoglobin) are varied in controlled amounts within the phantoms to simulate skin colour in d...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Widdowson DC,Shakespeare PG,Moore JC,Wright PA

    更新日期:2008-10-01 00:00:00

  • LLLT improves tendon healing through increase of MMP activity and collagen synthesis.

    abstract::The Achilles tendon has a high incidence of rupture, and the healing process leads to a disorganized extracellular matrix (ECM) with a high rate of injury recurrence. To evaluate the effects of different conditions of low-level laser (LLL) application on partially tenotomized tendons, adult male rats were divided into...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Guerra Fda R,Vieira CP,Almeida MS,Oliveira LP,de Aro AA,Pimentel ER

    更新日期:2013-09-01 00:00:00

  • Quantitative evaluation of skin shrinkage associated with non-invasive skin tightening: a simple method for reproducible linear measurement using microtattoos.

    abstract::Non-invasive skin-tightening devices can induce thermal denaturation and skin shrinkage via externally applied radiofrequency emissions or high-frequency ultrasound. Therefore, the purpose of this study is to develop and test a method for measurement of skin reduction associated with application of such energy devices...

    journal_title:Lasers in medical science

    pub_type: 杂志文章,随机对照试验


    authors: Alam M,Pongprutthipan M,Nanda S,Kim NA,Swary JH,Roongpisuthipong W,Kauvar AN,Weil A,Iyengar S,Chen BR,Vasic J,Maisel A,West DP,Nodzenksi M,Veledar E,Poon E

    更新日期:2019-06-01 00:00:00

  • Laser Nd:YAG patterning enhance human osteoblast behavior on zirconia implants.

    abstract::Zirconia has been regarded as a promising material for dental implants, and Nd:YAG laser treatment has been proposed as a potential strategy to improve its bioactivity. The main aim of the present study was to evaluate the in vitro behavior of human fetal osteoblasts in contact with laser-textured zirconia implant sur...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Fernandes BF,da Cruz MB,Marques JF,Madeira S,Carvalho Ó,Silva FS,da Mata ADSP,Caramês JMM

    更新日期:2020-12-01 00:00:00

  • Lasers for nevi: a review.

    abstract::A nevus is described as a benign, often pigmented, skin growth that can be congenital or acquired. Different types of nevi are located at different depths within the skin. Traditional treatments for nevi include topical treatments, dermabrasion, and excision, which are generally ineffective, invasive, and often result...

    journal_title:Lasers in medical science

    pub_type: 杂志文章,评审


    authors: Arora H,Falto-Aizpurua L,Chacon A,Griffith RD,Nouri K

    更新日期:2015-09-01 00:00:00

  • Temperature rise during photo-activated disinfection of root canals.

    abstract::The aim of this study was to determine whether it is safe to use photo-activated disinfection (PAD) during root canal treatment without heating the periodontal tissues. Root canals of 30 freshly extracted single-rooted teeth were prepared using ProFiles up to size ISO 40 and then filled with photo-sensitiser: tolonium...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Dickers B,Lamard L,Peremans A,Geerts S,Lamy M,Limme M,Rompen E,De Moor RJ,Mahler P,Rocca JP,Nammour S

    更新日期:2009-01-01 00:00:00

  • Phototherapy and resistance training prevent sarcopenia in ovariectomized rats.

    abstract::The aim of this study was to histologically and biochemically analyze the effects of light-emitting diode therapy (LEDT) associated with resistance training to prevent sarcopenia in ovariectomized rats. Forty female Wistar rats (12 months old, 295-330 g) were bilaterally ovariectomized and divided into four groups (n ...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Corazza AV,Paolillo FR,Groppo FC,Bagnato VS,Caria PH

    更新日期:2013-11-01 00:00:00

  • Characterization of dental caries by LIF spectroscopy with 404-nm excitation.

    abstract::The potential of laser-induced fluorescence (LIF) spectroscopy for the characterization of different stages of dental caries using 404-nm diode laser excitation was investigated. In vitro spectra from 16 sound, 10 noncavitated carious and 10 cavitated carious molar teeth were recorded on a miniature fibre-optic spectr...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Thomas SS,Jayanthi JL,Subhash N,Thomas J,Mallia RJ,Aparna G

    更新日期:2011-05-01 00:00:00

  • Effects of photobiomodulation therapy, pharmacological therapy, and physical exercise as single and/or combined treatment on the inflammatory response induced by experimental osteoarthritis.

    abstract::Osteoarthritis (OA) triggers increased levels of inflammatory markers, including prostaglandin (PG) E2 and proinflammatory cytokines. The elevation of cytokine levels is closely associated with increased articular tissue degeneration. Thus, the use of combination therapies may presumably be able to enhance the effects...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Tomazoni SS,Leal-Junior EC,Pallotta RC,Teixeira S,de Almeida P,Lopes-Martins RÁ

    更新日期:2017-01-01 00:00:00

  • Efficacy of low-level laser therapy in the management of orthodontic pain: a systematic review and meta-analysis.

    abstract::This review aimed to identify the efficacy of low-level laser therapy (LLLT) in the management of orthodontic pain. This systematic review and meta-analysis was carried out in accordance with Cochrane Handbook and the PRISMA statement. An extensive literature search for RCTs, quasi-RCTs, and CCTs was performed through...

    journal_title:Lasers in medical science

    pub_type: 杂志文章,meta分析,评审


    authors: He WL,Li CJ,Liu ZP,Sun JF,Hu ZA,Yin X,Zou SJ

    更新日期:2013-11-01 00:00:00

  • A review of monochromatic light devices for the treatment of alopecia areata.

    abstract::There are many laser technologies that are being tested that claim to support hair regrowth for patients with alopecia areata (AA). In this paper, we will determine whether the body of evidence supports the use of devices using monochromatic light sources to treat AA. Articles were gathered from PubMed, Embase, and th...

    journal_title:Lasers in medical science

    pub_type: 杂志文章,meta分析,评审


    authors: Darwin E,Arora H,Hirt PA,Wikramanayake TC,Jimenez JJ

    更新日期:2018-02-01 00:00:00

  • Different CO2 laser vaporization protocols for the therapy of oral precancerous lesions and precancerous conditions: a 10-year follow-up.

    abstract::Use of the CO(2) laser (λ = 10.6 μm, continuous wave, defocused) is an established procedure for the treatment of premalignant lesions. Through employment of the sp-mode as well as scanners, thermal laser effects can be reduced but, on the other hand, a lesser degree of destruction of dysplastic cells could lead to an...

    journal_title:Lasers in medical science

    pub_type: 临床试验,杂志文章


    authors: Deppe H,Mücke T,Hohlweg-Majert B,Hauck W,Wagenpfeil S,Hölzle F

    更新日期:2012-01-01 00:00:00

  • Low-level visible light (LLVL) irradiation promotes proliferation of mesenchymal stem cells.

    abstract::Low-level visible light irradiation was found to stimulate proliferation potential of various types of cells in vitro. Stem cells in general are of significance for implantation in regenerative medicine. The aim of the present study was to investigate the effect of low-level light irradiation on the proliferation of m...

    journal_title:Lasers in medical science

    pub_type: 杂志文章


    authors: Lipovsky A,Oron U,Gedanken A,Lubart R

    更新日期:2013-07-01 00:00:00