Differential effects of spermine on phosphatidylinositol 3-kinase and phosphatidylinositol phosphate 5-kinase.


:The metabolism of phosphoinositides plays an important role in the signal transduction pathways. We report here that naturally occurring polyamines affect the activities of phosphatidylinositol (PI) 3-kinase and PI 4-phosphate (PIP) 5-kinase differently. While polyamines inhibited the PI 3-kinase activity, they stimulated the activity of PIP 5-kinase in the order of spermine > spermidine > putrescine. Spermine inhibited the PI 3-kinase activity in a concentration-dependent manner with an IC50 of 100 microM. On the other hand, spermine (5 mM) stimulated the activity of PIP 5-kinase 2-3 fold. Kinetic studies of spermine-mediated inhibition of PI 3-kinase revealed that it was noncompetitive with respect to ATP. The effect of Mg2+ and PIP2 concentration on kinase activity was sigmoidal, with spermine inhibiting PI 3-kinase activity at all PIP2 concentrations. While 1 mM calcium stimulated PI 3-kinase activity at submaximal concentrations of Mg2+ (1.25 mM), inhibition was observed at optimal concentration of Mg2+ (2 mM). We propose that spermine may modulate the cellular signal by virtue of its differential effects on phosphoinositide kinases.


Life Sci


Life sciences


Singh SS,Chauhan A,Brockerhoff H,Chauhan VP




Has Abstract


1995-01-01 00:00:00














  • Telmisartan attenuates diabetic nephropathy progression by inhibiting the dimerization of angiotensin type-1 receptor and adiponectin receptor-1.

    abstract:AIMS:The heterodimerization of angiotensin II receptors (AT1R and AT2R) with adiponectin receptor AdipoR1 and AdipoR2 may instigate high glucose (HG)-induced renal tubulointerstitial injury. This study examined the effect of telmisartan on diabetic nephropathy (DN) and its underlying mechanism. MAIN METHODS:Diabetes w...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Zha D,Yao T,Bao L,Gao P,Wu X

    更新日期:2019-03-15 00:00:00

  • Effect of phenobarbital on metabolism of polyphosphoinositides of rat brain synaptosomes.

    abstract::Synthesis and degradation of polyphosphoinositides in a rat brain synaptosome preparation were depressed by phenobarbital. Phosphatidylinositol-4-phosphate kinase (PIP-kinase), the enzyme which synthesizes phosphatidylinositol-4,5-bisphosphate (PIP2) was most strongly affected (50% inhibition at 3 mM phenobarbital); p...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Deshmukh DS,Kuizon S,Brockerhoff H

    更新日期:1987-11-02 00:00:00

  • Long non-coding RNA TINCR as potential biomarker and therapeutic target for cancer.

    abstract::Despite the recent scientific advances made in cancer diagnostics and therapeutics, cancer still remains the second leading cause of death worldwide. Thus, there is a need to identify new potential biomarkers/molecular targets to improve the diagnosis and treatment of cancer patients. In this regard, long non-coding R...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Sharma U,Barwal TS,Malhotra A,Pant N,Vivek,Dey D,Gautam A,Tuli HS,Vasquez KM,Jain A

    更新日期:2020-09-15 00:00:00

  • Overexpression of GRIM-19 accelerates radiation-induced osteosarcoma cells apoptosis by p53 stabilization.

    abstract:AIMS:Osteosarcoma is one of the most aggressive types of primary bone cancer that responds poorly to radiotherapy frequently. The gene associated with retinoid-interferon mortality (GRIM-19) is a tumor suppressor that mediates cell apoptosis in multiple cancer types. However, the role of GRIM-19 in osteosarcoma and the...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Chen W,Liu Q,Fu B,Liu K,Jiang W

    更新日期:2018-09-01 00:00:00

  • Effects of metronidazole and its metabolites on histamine immunosuppression activity.

    abstract::We have previously reported that metronidazole treatment increases human lymphocyte proliferation showing individual differences. This drug and its metabolites are imidazole compounds like histamine and cimetidine. The first is an endogenous amine that inhibits T-helper lymphocyte proliferation, and the second is a hi...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Elizondo G,Ostrosky-Wegman P

    更新日期:1996-01-01 00:00:00

  • Role of p62/SQSTM1 in lipopolysaccharide (LPS)-induced mucus hypersecretion in bronchial epithelial cells.

    abstract:AIMS:The aims of this study were to evaluate the effects of p62/SQSTM1 expression levels on lipopolysaccharide (LPS)-induced mucus secretion in BEAS-2B bronchial epithelial cells by measuring expression levels of the MUC5AC gene and the Mucin-5AC (MUC5AC) protein. MATERIALS AND METHODS:Bronchial epithelial cells, BEAS...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Wu Y,Li Y,Wang B,He X,Li Y,Wu B,Yu G,Wang H,Xu B

    更新日期:2018-10-15 00:00:00

  • Effect of moxisylyte hydrochloride on isolated human penile corpus cavernosum tissue.

    abstract::The effect of moxisylyte hydrochloride on isolated human penile corpus cavernosum tissue was investigated and compared with other a-adrenergic antagonists. Moxisylyte produced a concentration-dependent relaxation of a norepinephrine-induced (1 x 10(-5) M) contraction of the corpus cavernosum tissue. Pretreatment with ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Imagawa A,Kimura K,Kawanishi Y,Tamura M

    更新日期:1989-01-01 00:00:00

  • Anti-curare effect of plasma from patients with thermal injury.

    abstract::Following severe thermal injury, patients are resistant to non-depolarizing muscle relaxants. Although this resistance has been well documented clinically, little is known about its etiology. We have tested the hypothesis that circulating factors contribute to the decreased potency of neuromuscular blockers following ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Storella RJ,Martyn JA,Bierkamper GG

    更新日期:1988-01-01 00:00:00

  • Silymarin-induced mitochondrial Ca2+ release.

    abstract::The effect of silymarin on different functions of mitochondria isolated from rat kidneys was studied. Addition of silymarin to mitochondria oxidizing succinate, induced stimulation of the respiratory State 4; while in mitochondria oxidizing NAD-dependent substrates, the drug produced inhibition of the oxygen consumpti...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Chávez E,Bravo C

    更新日期:1988-01-01 00:00:00

  • Optimal compressive force induces bone formation via increasing bone morphogenetic proteins production and decreasing their antagonists production by Saos-2 cells.

    abstract::Orthodontic tooth movement induced alveolar bone resorption and formation around the teeth applied mechanical force. Although mechanical force can promote bone formation, the molecular mechanism that underlies this phenomenon is not fully understood. The purposes of this study were to determine how mechanical stress a...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Mitsui N,Suzuki N,Maeno M,Yanagisawa M,Koyama Y,Otsuka K,Shimizu N

    更新日期:2006-05-01 00:00:00

  • Preparation of highly purified momordin II without ribonuclease activity.

    abstract::Momordin II, a ribosome-inactivating protein from Momordica charantia seeds, was purified by a procedure involving a series of chromatographies on S-Sepharose, Sephadex G-50, CM-Sepharose, and Red Sepharose columns. Highly purified momordin II inhibited cell-free protein synthesis, released adenine from rat liver ribo...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Valbonesi P,Barbieri L,Bolognesi A,Bonora E,Polito L,Stirpe F

    更新日期:1999-01-01 00:00:00

  • miR424-5p functions as an anti-oncogene in cervical cancer cell growth by targeting KDM5B via the Notch signaling pathway.

    abstract:AIMS:Aberrant expression of miRNAs exert the critical roles in carcinogenesis, including cervical cancer. Recent study corroborated the down-regulation of miR424-5p in uterine cervix adenocarcinoma. This research aimed to investigate the function and underlying mechanisms of miR424-5p in cervical cancer cell growth. M...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Zhou Y,An Q,Guo RX,Qiao YH,Li LX,Zhang XY,Zhao XL

    更新日期:2017-02-15 00:00:00

  • Tumor necrosis factor-alpha-mediated signal transduction in human neutrophils: involvement of sphingomyelin metabolites in the priming effect of TNF-alpha on the fMLP-stimulated superoxide production.

    abstract::We investigated the mechanism underlying the priming effect of TNF-alpha on fMLP-stimulated superoxide production in human neutrophils. TNF-alpha enhanced fMLP-stimulated superoxide production in a concentration-dependent manner. TNF-alpha also induced sphingomyelin (SM) hydrolysis and increased the formation of its m...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Niwa M,Kozawa O,Matsuno H,Kanamori Y,Hara A,Uematsu T

    更新日期:2000-01-01 00:00:00

  • Involvement of Na+ and Ca2+ channel activation and resultant nitric oxide synthesis in glutamate-mediated hypoxic injury in rat cerebrocortical slices.

    abstract::The role of Na+ and Ca2+ channels in glutamate-mediated hypoxic injury was investigated in slices of the rat cerebral cortex. Hypoxic injury was determined by mitochondrial reduction of 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyltetrazolium bromide after exposure of brain slices to 30-min of hypoxia/glucose deprivation fo...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Oka M,Itoh Y,Ukai Y

    更新日期:2000-09-29 00:00:00

  • A spinal site mediates opiate analgesia in frogs.

    abstract::The application of acetic acid to the hind leg of a frog will induce a spinally mediated wiping reflex only if the acetic acid concentration is above a certain threshold. By using this reflex as the basis of a test for nociception, we show that morphine sulfate is a potent analgesic in the frog when injected into the ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Stevens CW,Pezalla PD

    更新日期:1983-11-21 00:00:00

  • Influence of sympathectomy on alpha 2 adrenoceptor binding sites in canine blood vessels.

    abstract::The effects of denervation of alpha 2 adrenoceptor binding sites were examined in canine arteries and veins. Denervation of the lower abdominal aorta, renal and femoral arteries and femoral veins marked reduced vessel norepinephrine concentrations. Denervation had little effect on the concentration of alpha 2 adrenoce...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Bobik A,Anderson WP

    更新日期:1983-07-25 00:00:00

  • Brain neurotransmitter receptor-binding characteristics in rats after oral administration of haloperidol, risperidone and olanzapine.

    abstract::The present study was conducted to characterize the binding of neurotransmitter receptors (dopamine D(2), serotonin 5-HT(2), histamine H(1), adrenaline alpha(1) and muscarine M(l) receptors) in the rat's brain after the oral administration of haloperidol, risperidone, and olanzapine. Haloperidol at 1 and 3 mg/kg displ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Uchida S,Kato Y,Hirano K,Kagawa Y,Yamada S

    更新日期:2007-04-03 00:00:00

  • Induction of oxidative stress by the metabolites accumulating in 3-methylglutaconic aciduria in cerebral cortex of young rats.

    abstract::3-methylglutaconic (MGT), 3-methylglutaric (MGA) and occasionally 3-hydroxyisovaleric (OHIVA) acids accumulate in a group of diseases known as 3-methylglutaconic aciduria (MGTA). Although the clinical presentation of MGTA is mainly characterized by neurological symptoms, the mechanisms of brain damage in this disease ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Leipnitz G,Seminotti B,Amaral AU,de Bortoli G,Solano A,Schuck PF,Wyse AT,Wannmacher CM,Latini A,Wajner M

    更新日期:2008-03-12 00:00:00

  • Orchiectomy upregulates rabbit prostate peripheral benzodiazepine receptors.

    abstract::The effect of orchiectomy on peripheral benzodiazepine receptors (PBZr) in the rabbit prostate was studied. The mean PBZr density in the mitochondrial fraction isolated from prostates of intact (non castrated) rabbits was 4066 fmol/mg protein which following castration increased to 7236 fmol/mg protein (p less than 0....

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Batra S,Alenfall J

    更新日期:1992-01-01 00:00:00

  • Mechanisms underlying the supra-maximal thermogenesis elicited by aminophylline in rats.

    abstract::Previous studies showed that acute treatment with aminophylline (AMPY) significantly elevated maximum thermogenesis and improved cold tolerance in rats and man in severe cold. However, the exact mechanism by which AMPY enhances thermogenesis was unknown. Rats receiving enprofylline (ENPRO) (1.5 and 15 mg/kg, i.p.), a ...

    journal_title:Life sciences

    pub_type: 杂志文章


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    更新日期:1989-01-01 00:00:00

  • Effect of repeated allogeneic bone marrow mononuclear cell transplantation on brain injury following transient focal cerebral ischemia in rats.

    abstract:AIMS:Transplantation of bone marrow mononuclear cells (BMMCs) exerts neuroprotection against cerebral ischemia. We examined the therapeutic timepoint of allogeneic BMMC transplantation in a rat model of focal cerebral ischemia, and determined the effects of repeated transplantation outside the therapeutic window. MAIN...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Kamiya F,Ueda M,Nito C,Kamiya N,Inaba T,Suda S,Saito T,Muraga K,Katayama Y

    更新日期:2014-01-24 00:00:00

  • Characterization and distribution of 3H-(3MeHis2)thyrotropin releasing hormone receptors in rat brain.

    abstract::The characteristics and distribution of putative thyrotropin releasing hormone (TRH) receptors were studied in rat central nervous system using the TRH analogue 3H-(3MeHis2)TRH as a radiolabeled ligand. The analogue had a dissociation constant of 2.3 +/- 0.2 nM and a receptor density of 34 +/- 2 fm/mg protein in whole...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Simasko SM,Horita A

    更新日期:1982-05-24 00:00:00

  • miR-145-5p promotes trophoblast cell growth and invasion by targeting FLT1.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Lv Y,Lu X,Li C,Fan Y,Ji X,Long W,Meng L,Wu L,Wang L,Lv M,Ding H

    更新日期:2019-12-15 00:00:00

  • Anatomical changes of the GABAergic system in the inferior colliculus of the genetically epilepsy-prone rat.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Roberts RC,Ribak CE

    更新日期:1986-09-01 00:00:00

  • Proconflict effect of carbon dioxide inhalation in rats.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Cuccheddu T,Floris S,Serra M,Porceddu ML,Sanna E,Biggio G

    更新日期:1995-03-10 00:00:00

  • Ovarian nitric oxide synthase (NOS) gene expression during peripubertal development.

    abstract::Nitric oxide (NO) is generated from L-arginine by different isoforms of the enzyme nitric oxide synthase (NOS) and is known to be involved in mediating several biological functions, some of which are associated with reproduction. In this study, we examined the ability of the prepubertal ovary to express the inducible ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Srivastava V,Jones BJ,Dookwah H,Hiney JK,Dees WL

    更新日期:1997-01-01 00:00:00

  • Mucocutaneous cholinergic system is targeted in mustard-induced vesication.

    abstract::The purpose of this research is to elucidate the pharmacological mechanism mediating vesicating effects of sulfur mustard (HD) and identify an antidote to its action. HD causes blisters because epithelial cells lose their attachments. Epithelial cell adhesion is under control of the local cytotransmitter acetylcholine...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Grando SA

    更新日期:2003-03-28 00:00:00

  • Distinct molecular forms of opiate binding in the frog brain.

    abstract::In frog brain membranes, equilibrium saturation binding of the opiate agonist: 3H-etorphine and of the opiate antagonist: 3H-diprenorphine is biphasic. In the presence of sodium ions (120 mM), "kinetic conversions" (unchanged Bmax) are observed: high affinity binding of the 3H-agonist is decreased while high affinity ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Puget A,Jauzac P,Meunier JC

    更新日期:1983-01-01 00:00:00

  • Lipid peroxidation: a possible role in gastric damage induced by ethanol in rats.

    abstract::Gastric mucosal lipid peroxide levels, based on the amounts of thiobarbituric acid reacting substances, increased soon after oral application of absolute ethanol. On the other hand, gastric mucosal nonprotein sulfhydryl levels slightly but significantly decreased. Administration of 20% ethanol, a mild irritant which c...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Mizui T,Doteuchi M

    更新日期:1986-06-09 00:00:00

  • Computational guided drug repurposing for targeting 2'-O-ribose methyltransferase of SARS-CoV-2.

    abstract:AIMS:The recent outbreak of pandemic severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has led the world towards a global health emergency. Currently, no proper medicine or effective treatment strategies are available; therefore, repurposing of FDA approved drugs may play an important role in overcoming the ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Sharma K,Morla S,Goyal A,Kumar S

    更新日期:2020-10-15 00:00:00