Abstract:
BACKGROUND:Interaction of the CD11/CD18 complex on polymorphonuclear neutrophils (PMNs) and intercellular adhesion molecule (ICAM)-1 on endothelium is a critical event in PMN-mediated tissue injury. In addition, increased expression of ICAM-1 on type I pneumocytes has been identified in a variety of pulmonary disorders associated with PMN-induced inflammation. We hypothesized that ICAM-1 up-regulation is sufficient to promote cytotoxicity via activated PMNs. METHODS:The complementary DNA for human ICAM-1 was transfected into Chinese hamster ovarian (CHO) cells, which do not inherently express this adhesion receptor, by using the expression vector CD1.8. Fluorescence-activated cell sorter analysis revealed 62% CHO cell surface expression of ICAM-1. Wild type and transfected CHO cells were labeled with chromium 51 and exposed to quiescent or activated (1 mumol/L phorbol myristate acetate) PMNs for 4 hours. Subsets were pretreated with a monoclonal antibody to ICAM-1. PMN cytotoxicity was determined by specific percent 51Cr release. RESULTS:Incubation of quiescent PMNs with wild type and transfected CHO cells produced nominal cell lysis, 0.5% +/- 0.3% and 0.2% +/- 0.2%, respectively. Activated PMNs produced 13.6% +/- 3.2% versus 1.4% +/- 0.7% cell lysis, comparing transfected with wild type CHO cells, and 0.5% +/- 0.2% cell lysis after pretreatment with a monoclonal antibody to ICAM-1, p < 0.01. CONCLUSIONS:ICAM-1 up-regulation is sufficient to promote cytotoxicity via activated PMNs. This may represent a potential target for attenuating PMN-mediated injury to endothelial and other cell lines, including parenchyma.
journal_name
Surgeryjournal_title
Surgeryauthors
Barnett CC,Moore EE,Moore FA,Biffl WL,Smith MF Jr,Carl VSdoi
10.1016/s0039-6060(05)80320-4subject
Has Abstractpub_date
1995-08-01 00:00:00pages
171-5; discussion 176issue
2eissn
0039-6060issn
1532-7361pii
S0039-6060(05)80320-4journal_volume
118pub_type
杂志文章相关文献
SURGERY文献大全abstract:BACKGROUND:Tumor-associated lymphatic networks are the primary routes for tumor cell dissemination and metastasis. Behind the background of possible lymphangiogenesis-associated therapies, the clinical impact of lymphangiogenesis (measured by lymphatic microvessel density [LMVD]) and specific lymphovascular invasion (L...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2012.10.007
更新日期:2013-04-01 00:00:00
abstract:BACKGROUND:Vascular complications are major complications after living donor liver transplantation (LDLT), especially in biliary atresia (BA). Early diagnosis and treatment of portal vein thrombosis (PVT) may ensure optimal graft function and good recipient survival. Our aim was to find any association between pre- and...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2010.04.018
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:A left hepatic graft in living donor liver transplantation (LDLT) often has 2 thin and short hepatic arterial stumps, which makes hepatic artery (HA) reconstructions much more difficult to perform. Consequently, some investigators regard using a left graft as a contraindication to LDLT, whereas others report...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2009.06.028
更新日期:2010-06-01 00:00:00
abstract:BACKGROUND:Bacterial translocation has been implicated in the alteration of the immune response after shock and trauma. This study examined the effect of bacterial translocation on myelopoiesis after hemorrhagic shock in germ-free and conventional rats. METHODS:Awake, unrestrained germ-free and conventional rats were ...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract:BACKGROUND:Conjoined twins are rare developmental anomalies. There is a paucity of literature other than case reports and small case series. The aim of this study was to examine national outcomes and identify predictors of mortality in newborn conjoined twins. METHODS:We reviewed data on newborn conjoined twins from t...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2019.06.028
更新日期:2019-11-01 00:00:00
abstract::The effect of chronic electrical stimulation on muscle mass of pedicled diaphragmatic muscle grafts (DMGs) implanted into the right ventricle was studied in 21 dogs. Nineteen dogs, followed up to 70 days (mean, 34 days), were divided into three groups. In six dogs (control) the DMG was not stimulated. In six other dog...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract:BACKGROUND:Patients with colon cancer often present with obstruction. Large series have reported obstruction among the high-risk features, yet prospective data on its specific prognostic influence are lacking. We hypothesized that obstruction is an independent risk factor for poor prognosis in patients with stage III c...
journal_title:Surgery
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.surg.2018.06.044
更新日期:2018-12-01 00:00:00
abstract::Twenty dogs underwent 15 minutes of normothermic ischemic arrest and 30 minutes of reperfusion while on cardiopulmonary bypass. In 10 control dogs, the reperfusate blood was not modified. In 10 other dogs, the aorta was reclamped and the heart reperfused for 5 minutes with blood containing L-glutamate (0.026M). We mea...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00
abstract:BACKGROUND:Secondary peritonitis continues to carry a high mortality rate despite the aggressive use of imaging, drainage, and antibiotics. Although host factors and microbial burden contribute to the outcome of peritonitis, we propose a role for bacterial virulence as a determinant of outcome from peritonitis. Bacteri...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2012.06.022
更新日期:2013-01-01 00:00:00
abstract::A long-acting somatostatin analog, SMS 201-995, is now available to treat the hormonal manifestations of islet cell tumors. We report its use in a patient with a metastatic glucagonoma refractory to conventional therapy. This patient, who was severely disabled by the rash of necrolytic migratory erythema and brittle d...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract:BACKGROUND:This study was conducted to evaluate the effects of tumor removal and body weight loss on insulin resistance. METHODS:Insulin sensitivity was examined in 12 patients with cancer and stable weight and five patients with cancer and weight loss before operation, six patients after complete tumor removal, and t...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1994-07-01 00:00:00
abstract:BACKGROUND:Osteopontin acts thru myeloid zinc finger-1 and transforming growth factor-β to drive the adoption of a cancer-associated fibroblast phenotype by local mesenchymal stem cells. Cancer-associated fibroblasts increase cancer cell stemness. METHODS:Mesenchymal stem cells were exposed to osteopontin or were cocu...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2019.05.042
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:Surgical procedures suppress host antitumor defense mechanisms, which may increase the risk of metastatic tumor dissemination. We have evaluated the effects of the biologic response modifier polyadenylic-polyuridylic acid (PAPU) on natural cytotoxicity in patients with breast cancer undergoing operation. ME...
journal_title:Surgery
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0039-6060(05)80370-8
更新日期:1995-09-01 00:00:00
abstract:BACKGROUND:Apoptotic cell death and c-Jun N-terminal kinase (JNK) activation occur after hepatic ischemia/reperfusion injury. In other cell types, JNK activation was shown to be required for apoptosis. This study tested the hypotheses that JNK contributes to hepatocellular apoptosis, and that inhibition of JNK activity...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1067/msy.2003.237
更新日期:2003-08-01 00:00:00
abstract::Previous studies performed in our laboratory with a constant-pressure model of hemorrhagic shock in the anesthetized rat have failed to find any significant effect of shock on the glycogen or high-energy phosphate content of the soleus muscle that would be consistent with inadequate oxygen supply. The present study ex...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
abstract:BACKGROUND:After portal vein ligation of 1 side of the liver, the other side regenerates at a slow rate. This slow growth may be accelerated to rapid growth by adding a transection between the 2 sides, i.e., performing portal vein ligation and parenchymal transection. We found that in patients undergoing portal vein li...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2016.05.018
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:Surgical success has traditionally been judged from the surgeon's perspective. A more complete evaluation of outcome incorporates the patient's, surgeon's, and payor's perspectives. Because gastroesophageal reflux disease (GERD) is primarily a quality-of-life (QOL) problem, the evaluation of laparoscopic fun...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1067/msy.2001.118092
更新日期:2001-10-01 00:00:00
abstract::Over a three year period, 28 children or young adults up to 20 years of age underwent 60 thoracotomies for pulmonary metastatic osteogenic sarcoma. These patients also received adjuvant chemotherapy. Sixteen of the 28 patients are currently alive and 13 of these are free of disease. The patients have been followed for...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1978-09-01 00:00:00
abstract:BACKGROUND:The relationship among bile duct (BD), portal vein (PV), and/or hepatic artery (HA) anatomy is controversial, but no investigation has previously compared left and right liver graft transplantation in terms of variant vasculobiliary anatomy. Therefore, in this study, we explored whether variant BD anatomy ca...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2009.06.017
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:Approximately 10% of patients taking lithium for manic-depressive disorders become hypercalcemic. It remains unclear whether lithium initiates disease or promotes underlying hyperparathyroidism. We have previously demonstrated that at therapeutic concentrations lithium stimulates in vitro incorporation of bo...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/s0039-6060(05)80258-2
更新日期:1995-05-01 00:00:00
abstract::The blood lost through the suture line holes of vessels closed with braided polyester sutures is compared with that lost when a monofilament polypropylene suture is employed. In a retrospective analysis of blood replacement during saphenous vein aortorenal bypasses, twice as much blood replacement was required when th...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00
abstract:BACKGROUND:Surgical enteral access prior to multimodality treatment for esophageal cancer is controversial as dysphagia is often used for feeding tube referral. We hypothesized that enteral access before neoadjuvant chemoradiation for esophageal cancer provides no benefit compared to that placed during definitive esoph...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2017.09.046
更新日期:2018-04-01 00:00:00
abstract::We investigated the effects of infusion of somatostatin (200 and 500 ng/kg/min) on central hemodynamics and renal blood flow measured with radioactive microspheres. At the lower dose infusion rate somatostatin did not alter any hemodynamic parameter, but at 500 ng/kg/min somatostatin caused minor transient bradycardia...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
abstract::The purpose of this study was to develop a method that would provide baseline data for the ongoing assessment of surgical clerkships at The University of Michigan Medical School. Of specific concern was the comparability of clerkship experiences among five different sites and the degree of variability in student exper...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1982-06-01 00:00:00
abstract:BACKGROUND:The Hedgehog (Hh) gene family is known to regulate development of stem cells. In addition, activation is responsible for the induction of GLI1 proto-oncogene and subsequent cellular proliferation. Sonic Hedgehog (SHh), one of the Hh family members promotes carcinogenesis in airway and pancreatic epithelia, i...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2005.10.012
更新日期:2006-05-01 00:00:00
abstract:BACKGROUND:Longitudinal, risk-adjusted measurement of outcomes of carotid artery (CA) surgery is necessary for the evaluation of quality performance and for the assessment of strategies of quality improvement. METHODS:Patients from quality coding hospitals who underwent CA surgery and met procedural and diagnostic cod...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2015.06.005
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:The objectives of the present study are to determine the long-term changes in glucose tolerance function after pancreaticoduodenectomy and to compare the effects of pancreaticojejunostomy (PJ) and pancreaticogastrostomy (PG). Patients and methods The present study consisted of 51 patients who received a panc...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2004.01.006
更新日期:2004-09-01 00:00:00
abstract::A patient with an uncommon cause of portal venous hypertension, pancreatitis, is depicted. The patient had an equally uncommon pattern of symptoms and signs consisting of abdominal pain and lower gastrointestinal hemorrhage caused by colonic varices. A unique treatment, with angiographic placement of an expandable int...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::We investigated the active enhancement of liver allografts in 44 pairs of beagle dogs by pretreatment with semisoluble and soluble donor-specific spleen antigens. If the antigen were administered together with prednisolone, time of survival was significantly prolonged--with soluble antigen by 36.8 +/- 5.9 days and wit...
journal_title:Surgery
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract:BACKGROUND:Aggressive screening for blunt cerebrovascular injury (BCVI) and prompt anticoagulation for documented injuries has resulted in a significant reduction in ischemic neurologic events. An association between vertebral artery injuries (VAIs) and specific cervical spine fracture patterns has been suggested; howe...
journal_title:Surgery
pub_type: 杂志文章
doi:10.1016/j.surg.2006.04.005
更新日期:2007-01-01 00:00:00