Modulation of very late activation-2 laminin receptor function in breast cancer metastasis.


BACKGROUND:Very late activation-2 (VLA-2) is an integrin receptor for laminin that consists of an alpha 2- and a beta 1-subunit. In human breast cancer, down-regulation of VLA-2 expression is related to positive nodal status. The functional significance of altered integrin expression in individual patients has never been investigated. To test the hypothesis that less adhesive primary breast cancer cells were predisposed to metastasize, variation in VLA-2 modulation of cell attachment to laminin with nodal status was studied. METHODS:Integrin expression was measured by means of immunohistochemistry on cryostat sections. Primary breast cancer cells were isolated by enzymatic disaggregation and immunomagnetic separation. Cell adhesion to laminin was evaluated in an in vitro assay, and the effect of monoclonal antibodies against the component subunits of VLA-2 was assessed. RESULTS:Adhesion of primary breast cancer cells from women with positive nodes to laminin was significantly reduced compared with women with negative nodes (p < 0.001, Wilcoxon signed rank test). VLA-2 antibodies inhibited primary breast cancer cell attachment of women with negative nodes but not women with positive nodes. Strong adhesion to laminin was related to node-negative status (chi-squared, 16.33; p < 0.001) and to positive integrin expression (chi-squared, 31.54; p < 0.001). CONCLUSIONS:VLA-2-mediated adhesion of primary breast cancer cells to laminin differs with nodal status. Measurement of VLA-2 expression may thus be of clinical value as a prognostic indicator in the assessment of breast cancer.






Gui GP,Puddefoot JR,Vinson GP,Wells CA,Carpenter R




Has Abstract


1995-08-01 00:00:00
















  • Sleep disturbances before and after parathyroidectomy for secondary hyperparathyroidism.

    abstract:BACKGROUND:The prevalence of insomnia in patients with symptomatic secondary hyperparathyroidism and the improvements of sleep disturbances after parathyroidectomy have not been previously reported. METHODS:Thirty-one patients who had undergone successful total parathyroidectomy and autotransplantation for symptomatic...


    pub_type: 杂志文章


    authors: Chou FF,Lee CH,Chen JB,Huang SC,Lee CT

    更新日期:2005-04-01 00:00:00

  • Morbidity and mortality in patients with esophageal atresia.

    abstract:BACKGROUND:This study reports national estimates of population characteristics and outcomes for patients with esophageal atresia with or without tracheoesophageal fistula (EA/TEF) and evaluates the relationships between hospital volume and outcomes. METHODS:Patients admitted within 30 days of life who had Internationa...


    pub_type: 杂志文章,多中心研究


    authors: Sulkowski JP,Cooper JN,Lopez JJ,Jadcherla Y,Cuenot A,Mattei P,Deans KJ,Minneci PC

    更新日期:2014-08-01 00:00:00

  • Screening for primary hyperparathyroidism before thyroid surgery: A prospective study.

    abstract:BACKGROUND:Misdiagnosed primary hyperparathyroidism (PHPT) during thyroid surgery may lead to a difficult reoperation. Because PHPT is often asymptomatic, calcium measurements have been recommended before thyroid surgery, but no study has focused on the results of a prospective PHPT screening. METHODS:The prospective ...


    pub_type: 杂志文章


    authors: Denizot A,Dadoun F,Meyer-Dutour A,Alliot P,Argème M

    更新日期:2002-03-01 00:00:00

  • Lymphangiogenesis and lymphovascular invasion diminishes prognosis in esophageal cancer.

    abstract:BACKGROUND:Tumor-associated lymphatic networks are the primary routes for tumor cell dissemination and metastasis. Behind the background of possible lymphangiogenesis-associated therapies, the clinical impact of lymphangiogenesis (measured by lymphatic microvessel density [LMVD]) and specific lymphovascular invasion (L...


    pub_type: 杂志文章


    authors: Schoppmann SF,Jesch B,Zacherl J,Riegler MF,Friedrich J,Birner P

    更新日期:2013-04-01 00:00:00

  • Deep learning-based computer vision to recognize and classify suturing gestures in robot-assisted surgery.

    abstract:BACKGROUND:Our previous work classified a taxonomy of suturing gestures during a vesicourethral anastomosis of robotic radical prostatectomy in association with tissue tears and patient outcomes. Herein, we train deep learning-based computer vision to automate the identification and classification of suturing gestures ...


    pub_type: 杂志文章


    authors: Luongo F,Hakim R,Nguyen JH,Anandkumar A,Hung AJ

    更新日期:2020-09-26 00:00:00

  • Extracorporeal removal of anticancer drugs in hepatic artery infusion: the effect of direct hemoperfusion combined with venovenous bypass.

    abstract::The effect of a new extracorporeal system combining direct hemoperfusion (DHP) with venovenous bypass was evaluated in the elimination of anticancer drugs in hepatic artery infusion. Adriamycin (3 mg/kg) and mitomycin C (1 mg/kg) were given to mongrel dogs through the hepatic artery with three different durations of 1...


    pub_type: 杂志文章


    authors: Ku Y,Saitoh M,Nishiyama H,Fujiwara S,Iwasaki T,Tominaga M,Maekawa Y,Ohyanagi H,Saitoh Y

    更新日期:1990-03-01 00:00:00

  • Nonoperative observation of clinically occult arterial injuries: a prospective evaluation.

    abstract::Forty-seven patients with 50 clinically occult injuries of major arteries were studied prospectively to determine the natural history of these lesions and the safety of nonoperative management. Penetrating trauma was the predominant mechanism and lower extremity arteries were most commonly involved. The morphology of ...


    pub_type: 杂志文章


    authors: Frykberg ER,Crump JM,Dennis JW,Vines FS,Alexander RH

    更新日期:1991-01-01 00:00:00

  • Dextrose containing intravenous fluid impairs outcome and increases death after eight minutes of cardiac arrest and resuscitation in dogs.

    abstract::Use of dextrose in intravenous resuscitation fluids is common practice; however, this study indicates that 5% dextrose solutions, even if administered in physiologic quantities, greatly worsens the outcome of survivable cardiac arrest. Twelve adult male mongrel dogs were premedicated with morphine, anesthetized with h...


    pub_type: 杂志文章


    authors: D'Alecy LG,Lundy EF,Barton KJ,Zelenock GB

    更新日期:1986-09-01 00:00:00

  • Virilizing adrenocortical adenoma: in vitro steroidogenesis, immunohistochemical studies of steroidogenic enzymes, and gene expression of corticotropin receptor.

    abstract:BACKGROUND:No reports have yet precisely determined corticotropin (ACTH) responsiveness in virilizing adrenocortical adenoma. METHODS:Five women with an androgen-secreting adrenal adenoma were reviewed. Three of them were examined by in vitro steroidogenesis. Two of these 3 patients were studied by immunohistochemistr...


    pub_type: 杂志文章


    authors: Imai T,Tobinaga J,Morita-Matsuyama T,Kikumori T,Sasano H,Seo H,Funahashi H

    更新日期:1999-04-01 00:00:00

  • Decrease in core liver temperature with 10 degrees C by in situ hypothermic perfusion under total hepatic vascular exclusion reduces liver ischemia and reperfusion injury during partial hepatectomy in pigs.

    abstract:OBJECTIVE:We attempted to assess liver ischemia/reperfusion injury under a mild decrease in core liver temperature of 10 degrees C by in situ hypothermic perfusion during ischemia. METHODS:Liver ischemia was induced in pigs by total hepatic vascular exclusion with concomitant in situ perfusion with hypothermic (4 degr...


    pub_type: 杂志文章


    authors: Heijnen BH,Straatsburg IH,Gouma DJ,van Gulik TM

    更新日期:2003-11-01 00:00:00

  • Not all Whipple procedures are equal: Proposal for a classification of pancreatoduodenectomies.

    abstract:BACKGROUND:Pancreatoduodenectomy is the standard treatment for pathologies of the pancreatic head and is performed routinely worldwide. The aim of the study was to analyze this procedure in terms of extent of surgery, technical difficulty, and clinical outcomes and thereby provide a standardized surgical categorization...


    pub_type: 杂志文章


    authors: Mihaljevic AL,Hackert T,Loos M,Hinz U,Schneider M,Mehrabi A,Hoffmann K,Berchtold C,Müller-Stich BP,Diener M,Strobel O,Büchler MW

    更新日期:2020-12-29 00:00:00

  • Influence of portal blood on the development of systemic inflammation associated with experimental acute pancreatitis.

    abstract:BACKGROUND:The liver is a source of systemic proinflammatory mediators in acute pancreatitis. We have investigated the effects of blood from the pancreas and intestine in liver activation and lung inflammation during early stages of experimental acute pancreatitis in a rat model. METHODS:A portosystemic shunt and a me...


    pub_type: 杂志文章


    authors: Hoyos S,Granell S,Heredia N,Bulbena O,Closa D,Fernández-Cruz L

    更新日期:2005-02-01 00:00:00

  • Effects of perfluorocarbon exchange transfusion on reducing myocardial infarct size in a primate model of ischemia-reperfusion injury: a prospective, randomized study.

    abstract:BACKGROUND:This study was undertaken to test the hypothesis that perfluorocarbons were able to reduce myocardial infarct size in a baboon model of ischemia-reperfusion injury. Exchange transfusion of perfluorocarbons has been shown to reduce myocardial infarct size in the dog, who, unlike the baboon, has an extensive c...


    pub_type: 杂志文章


    authors: Premaratne S,Harada RN,Chun P,Suehiro A,McNamara JJ

    更新日期:1995-06-01 00:00:00

  • Pathological fibrinolysis secondary to pseudoaneurysms.

    abstract::Pathological fibrinolysis due to pseudoaneurysms was observed in a patient 4 years after aortobifemoral bypass graft. The patient presented with a pulsatile abdominal mass and ischemic changes in the legs. Excessive bleeding from venipuncture sites prompted coagulation screening, which disclosed rapid clot lysis, fibr...


    pub_type: 杂志文章


    authors: Babu SC,Bole P,Sharma P,Purdy R,Clauss RH

    更新日期:1980-02-01 00:00:00

  • Intraoperative sestamibi scanning in reoperative parathyroidectomy.

    abstract:BACKGROUND:Reoperative neck exploration for hyperparathyroidism is often difficult even for experienced surgeons. Recent advances in preoperative and intraoperative localization techniques have improved successful resection rates. This prospective study evaluates the accuracy and clinical utility of intraoperative tech...


    pub_type: 临床试验,杂志文章


    authors: Rossi HL,Ali A,Prinz RA

    更新日期:2000-10-01 00:00:00

  • Cognitive changes after parathyroidectomy in patients with secondary hyperparathyroidism.

    abstract:BACKGROUND:Cognitive impairment was frequently reported in uremic patients with dialysis, but improvements of cognition after parathyroidectomy for symptomatic secondary hyperparathyroidism have never been reported before. METHODS:Thirty-nine patients, who were successfully operated on with total parathyroidectomy plu...


    pub_type: 杂志文章


    authors: Chou FF,Chen JB,Hsieh KC,Liou CW

    更新日期:2008-04-01 00:00:00

  • A pathogenesis for alcoholic pancreatitis.

    abstract::Twelve patients with prior episodes of alcoholic pancreatitis and hyperlipemia were admitted to a metabolic ward during a quiescent period. By lipid feeding (316 to 894 Gm. per day), significant hypertriglyceridemia (greater than 600 mg. per 100 ml.) was induced in 11 of the 12 patients. Seven of the 11 patients with ...


    pub_type: 杂志文章


    authors: Cameron JL,Zuidema GD,Margolis S

    更新日期:1975-06-01 00:00:00

  • Virilizing hilus (Leydig) cell tumor of the ovary: the challenge of an accurate preoperative diagnosis.

    abstract::A 73-year-old woman developed temporal balding and "hoarseness" over a 3-year interval. Investigation including extensive endocrinologic screening, abdominal computed tomography scanning, pelvic ultrasonography, iodocholesterol (NP-59) adrenal scanning, and selective retrograde venous sampling revealed only an elevate...


    pub_type: 杂志文章


    authors: Raaf JH,Bajorunas DR,Smith DH,Woodruff J

    更新日期:1983-12-01 00:00:00

  • Females have fewer complications and lower mortality following trauma than similarly injured males: a risk adjusted analysis of adults in the National Trauma Data Bank.

    abstract:BACKGROUND:Studies of sexual dimorphism in trauma outcomes suggest that women have a survival advantage compared to equivalently injured men. It is unknown if this gender disparity is mediated by potentially life-threatening complications. OBJECTIVE:To determine (1) if there is a sex-based differences in the odds of d...


    pub_type: 杂志文章


    authors: Haider AH,Crompton JG,Oyetunji T,Stevens KA,Efron DT,Kieninger AN,Chang DC,Cornwell EE 3rd,Haut ER

    更新日期:2009-08-01 00:00:00

  • Application of an individualized operative strategy for wedge resection of gastric gastrointestinal stromal tumors: Effectiveness for tumors in difficult locations.

    abstract:BACKGROUND:There is some concern that wedge resection of gastric gastrointestinal stromal tumors is not feasible in certain anatomic locations, such as the cardia or antrum. We sought to review our experience with treatment of gastric gastrointestinal stromal tumors with a particular focus on nonanatomic wedge resectio...


    pub_type: 杂志文章


    authors: Mueller CL,Braun J,Leimanis ML,Mouhanna J,Feldman LS,Ferri LE

    更新日期:2016-10-01 00:00:00

  • Learning sentinel node biopsy: results of a prospective randomized trial of two techniques.

    abstract:BACKGROUND:Evidence indicates that sentinel node (SN) biopsy can accurately predict axillary nodal status. Debate exists as to the optimal method of SN identification. METHODS:Patients with clinical T1 or T2 tumors and negative axillae were randomized to SN localization with blue dye (B) alone (n = 50) or blue dye plu...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Morrow M,Rademaker AW,Bethke KP,Talamonti MS,Dawes LG,Clauson J,Hansen N

    更新日期:1999-10-01 00:00:00

  • Desmoid tumor: musculoaponeurotic fibrosis of the abdominal wall.

    abstract::A 26-year-old woman developed a desmoid tumor of the lower abdominal wall shortly after the birth of her fourth child. It measured 15 by 17 cm. and involved most of the lower abdominal wall of this small-framed woman. Operative removal would have been mutilating and almost surely doomed to failure. No treatment was gi...


    pub_type: 杂志文章


    authors: Caldwell EH

    更新日期:1976-01-01 00:00:00

  • Attenuation of hemodynamic and hematologic effects of heparin-protamine sulfate interaction after aortic reconstruction in a canine model.

    abstract::This investigation documented the effect of protamine sulfate pretreatment on the adverse hemodynamic and hematologic sequela of rapid intravenous protamine administration in heparinized dogs having undergone prior implantation of aortic prostheses. Fourteen dogs underwent infrarenal aortic replacement with 6 mm inner...


    pub_type: 杂志文章


    authors: Wakefield TW,Lindblad B,Whitehouse WM Jr,Hantler CB,Stanley JC

    更新日期:1986-07-01 00:00:00

  • Identification of multiple endocrine neoplasia type 1 in patients with apparent sporadic primary hyperparathyroidism.

    abstract:BACKGROUND:In the evaluation of patients with primary hyperparathyroidism (PHP), specific query for a personal or family history of MEN1 (Hx) is recommended widely, but responses are rarely positiv. We instituted a 6-question panel (6Q) to routinely screen for MEN1 preoperatively. METHODS:The clinical database entries...


    pub_type: 杂志文章


    authors: Yip L,Ogilvie JB,Challinor SM,Salata RA,Thull DL,Yim JH,Carty SE

    更新日期:2008-12-01 00:00:00

  • Time to first take-back operation predicts successful primary fascial closure in patients undergoing damage control laparotomy.

    abstract:BACKGROUND:Failure to achieve primary fascial closure (PFC) after damage control laparotomy is costly and carries great morbidity. We hypothesized that time from the initial laparotomy to the first take-back operation would be predictive of successful PFC. METHODS:Trauma patients managed with open abdominal techniques...


    pub_type: 临床试验,杂志文章,多中心研究


    authors: Pommerening MJ,DuBose JJ,Zielinski MD,Phelan HA,Scalea TM,Inaba K,Velmahos GC,Whelan JF,Wade CE,Holcomb JB,Cotton BA,AAST Open Abdomen Study Group.

    更新日期:2014-08-01 00:00:00

  • Radiologic characterization of adrenal masses: the role of computed tomography--derived attenuation values.

    abstract:BACKGROUND:Recent studies suggest that low computed tomography (CT) attenuation values can be used to differentiate benign adrenal adenomas from non-adenomas. We examined the utility of non-enhanced CT attenuation values of


    pub_type: 杂志文章


    authors: Nwariaku FE,Champine J,Kim LT,Burkey S,O'keefe G,Snyder WH 3rd

    更新日期:2001-12-01 00:00:00

  • Acute limb ischemia associated with type B aortic dissection: clinical relevance and therapy.

    abstract:BACKGROUND:The goal of the current study is to characterize the presentation, therapy, and outcomes of acute limb ischemia (ALI) associated with type B aortic dissection (AoD). METHODS:The prospective/retrospective International Registry for Acute Aortic Dissection (IRAD) database and a single institutional database w...


    pub_type: 杂志文章


    authors: Henke PK,Williams DM,Upchurch GR Jr,Proctor M,Cooper JV,Fang J,Nienaber CA,Isselbacher EM,Fattori R,Dasika N,Gemmete J,Stanley JC,Wakefield TW,Eagle KA

    更新日期:2006-10-01 00:00:00

  • Hepatic uptake index in the hepatobiliary phase of gadolinium ethoxybenzyl diethylenetriamine penta acetic acid-enhanced magnetic resonance imaging estimates functional liver reserve and predicts post-hepatectomy liver failure.

    abstract:BACKGROUND:Recent evidence suggests that gadolinium ethoxybenzyl diethylenetriamine penta acetic acid-enhanced (Gd-EOB-DTPA) magnetic resonance imaging may be used to evaluate liver function. The aim of this study was to assess whether the signal intensity of Gd-EOB-DTPA magnetic resonance imaging may be used to predic...


    pub_type: 杂志文章


    authors: Donadon M,Lanza E,Branciforte B,Muglia R,Lisi C,Pedicini V,Poretti D,Famularo S,Balzarini L,Torzilli G

    更新日期:2020-09-01 00:00:00

  • Matas, Antyllus, and endoaneurysmorrhaphy.

    abstract::Much can be learned about the history of vascular surgery by tracing the treatment of aneurysms. Rudolph Matas played a major role in the development of the current therapy for these lesions. In the centennial of the signal case that set him on the road to surgical immortality, the details and setting of this operatio...


    pub_type: 传,历史文章,杂志文章


    authors: Friedman SG,Friedman MS

    更新日期:1989-06-01 00:00:00

  • The impact of in-hospital complications on the long-term functional outcome of trauma patients: A multicenter study.

    abstract:BACKGROUND:The long-term consequences of in-hospital complications remain largely unknown. We sought to study the effect of complications on the long-term functional outcome of trauma patients. METHODS:Patients with an Injury Severity Score ≥ 9 admitted to 3, level I trauma centers between 2015 and 2017 were contacted...


    pub_type: 杂志文章,多中心研究


    authors: Lee JM,Herrera-Escobar J,Apoj M,Al Rafai SS,Han K,Nehra D,Salim A,Brasel K,Kasotakis G,Velmahos G,Haider A,Kaafarani HMA

    更新日期:2019-09-01 00:00:00