Modulation of very late activation-2 laminin receptor function in breast cancer metastasis.


BACKGROUND:Very late activation-2 (VLA-2) is an integrin receptor for laminin that consists of an alpha 2- and a beta 1-subunit. In human breast cancer, down-regulation of VLA-2 expression is related to positive nodal status. The functional significance of altered integrin expression in individual patients has never been investigated. To test the hypothesis that less adhesive primary breast cancer cells were predisposed to metastasize, variation in VLA-2 modulation of cell attachment to laminin with nodal status was studied. METHODS:Integrin expression was measured by means of immunohistochemistry on cryostat sections. Primary breast cancer cells were isolated by enzymatic disaggregation and immunomagnetic separation. Cell adhesion to laminin was evaluated in an in vitro assay, and the effect of monoclonal antibodies against the component subunits of VLA-2 was assessed. RESULTS:Adhesion of primary breast cancer cells from women with positive nodes to laminin was significantly reduced compared with women with negative nodes (p < 0.001, Wilcoxon signed rank test). VLA-2 antibodies inhibited primary breast cancer cell attachment of women with negative nodes but not women with positive nodes. Strong adhesion to laminin was related to node-negative status (chi-squared, 16.33; p < 0.001) and to positive integrin expression (chi-squared, 31.54; p < 0.001). CONCLUSIONS:VLA-2-mediated adhesion of primary breast cancer cells to laminin differs with nodal status. Measurement of VLA-2 expression may thus be of clinical value as a prognostic indicator in the assessment of breast cancer.






Gui GP,Puddefoot JR,Vinson GP,Wells CA,Carpenter R




Has Abstract


1995-08-01 00:00:00
















  • Growth hormone together with glutamine-containing total parenteral nutrition maintains muscle glutamine levels and results in a less negative nitrogen balance after surgical trauma.

    abstract:BACKGROUND:Muscle protein catabolism, reflected by a decrease in glutamine (GLN), a decrease in muscle protein synthesis, and a negative nitrogen balance can be reduced by either administration of GLN or growth hormone (GH). In this study, the effects of a combination of GH and GLH were studied. METHODS:Patients (n = ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Hammarqvist F,Sandgren A,Andersson K,Essén P,McNurlan MA,Garlick PJ,Wernerman J

    更新日期:2001-05-01 00:00:00

  • The value of spleen-preserving lymphadenectomy in total gastrectomy for gastric and esophagogastric junctional adenocarcinomas: A long-term retrospective propensity score match study from a high-volume institution in China.

    abstract:BACKGROUND:The benefit of removing the splenic lymph nodes in patients with proximal gastric cancer has been controversial. The purpose of our study was to investigate the importance of performing a splenic hilar lymph node dissection without splenectomy in patients undergoing total gastrectomy for gastric cancer. MET...


    pub_type: 杂志文章


    authors: Liu K,Chen XZ,Zhang YC,Zhang WH,Chen XL,Sun LF,Yang K,Zhang B,Zhou ZG,Hu JK

    更新日期:2021-02-01 00:00:00

  • Hyperfunctioning intrathyroid parathyroid gland: a potential cause of failure in parathyroidectomy.

    abstract:BACKGROUND:The surgical success rate of parathyroidectomy is 95%. However, in rare patients, ectopic hyperfunctioning parathyroid glands may be located in the thyroid but missed on imaging studies or during surgery. The aim of the present study was to evaluate the perioperative findings in 6 patients with intrathyroid ...


    pub_type: 杂志文章


    authors: Bahar G,Feinmesser R,Joshua BZ,Shpitzer T,Morgenstein S,Popovtzer A,Shvero J

    更新日期:2006-06-01 00:00:00

  • Cytokine induction of interferon regulatory factor-1 in hepatocytes.

    abstract:BACKGROUND:Interferon regulatory factor-1 (IRF-1) is a transcriptional factor originally cloned from fibroblasts that activates interferons and certain interferon-responsive genes. Because IRF-1 is an "early-immediate" nuclear protein, it can function acutely after trauma or septic stimuli. We have identified IRF-1 exp...


    pub_type: 杂志文章


    authors: Geller DA,Nguyen D,Shapiro RA,Nussler A,Di Silvio M,Freeswick P,Wang SC,Tweardy DJ,Simmons RL,Billiar TR

    更新日期:1993-08-01 00:00:00

  • Improved outcome of adult blunt splenic injury: a cohort analysis.

    abstract:BACKGROUND:The purpose of this study was to review our 15-year experience in the treatment of blunt splenic injury in adults. Our hypothesis was that the implementation of a change in practice, with stress on splenic preservation and splenic artery embolization for the management of splenic injury, would result in impr...


    pub_type: 杂志文章


    authors: Rajani RR,Claridge JA,Yowler CJ,Patrick P,Wiant A,Summers JI,McDonald AA,Como JJ,Malangoni MA

    更新日期:2006-10-01 00:00:00

  • Predictive factors of mortality in bariatric surgery: data from the Nationwide Inpatient Sample.

    abstract:BACKGROUND:Understanding predictors of mortality in bariatric surgery enables surgeons to use these factors for analysis of risk-adjusted mortality and aids in the surgical decision making and informed consent process. OBJECTIVES:To evaluate the effect of patient characteristics (age, gender, race, and payer type), pr...


    pub_type: 杂志文章


    authors: Nguyen NT,Masoomi H,Laugenour K,Sanaiha Y,Reavis KM,Mills SD,Stamos MJ

    更新日期:2011-08-01 00:00:00

  • Peptide receptor radionuclide therapy as neoadjuvant therapy for resectable or potentially resectable pancreatic neuroendocrine neoplasms.

    abstract:BACKGROUND:Peptide receptor radionuclide therapy is a valid therapeutic option for pancreatic neuroendocrine neoplasms. The aim of this study was to describe an initial experience with the use of peptide receptor radionuclide therapy as a neoadjuvant agent for resectable or potentially resectable pancreatic neuroendocr...


    pub_type: 杂志文章


    authors: Partelli S,Bertani E,Bartolomei M,Perali C,Muffatti F,Grana CM,Schiavo Lena M,Doglioni C,Crippa S,Fazio N,Zamboni G,Falconi M

    更新日期:2018-04-01 00:00:00

  • Changes in hypothalamic neuropeptide Y and monoaminergic system in tumor-bearing rats: pre- and post-tumor resection and at death.

    abstract:BACKGROUND:Cancer anorexia is influenced by the neuropeptidergic and monoaminergic systems. We hypothesize that serotonin (5-HT), dopamine (DA) and neuropeptide Y (NPY) concentrations in paraventricular (PVN), ventromedial (VMN), and lateral hypothalamus (LHA) areas are abnormal in tumor-bearing rats. METHODS:Fifty-fi...


    pub_type: 杂志文章


    authors: Ramos EJ,Suzuki S,Meguid MM,Laviano A,Sato T,Chen C,Das U

    更新日期:2004-08-01 00:00:00

  • Growth hormone attenuates the abnormal distribution of body water in critically ill surgical patients.

    abstract:BACKGROUND:Catabolic illness is associated with fluid retention and extracellular space expansion. To determine the effect of human growth hormone (GH) on body water compartments, critically ill surgical patients were studied for a 2-week period during which they either continued to receive standard intensive care unit...


    pub_type: 临床试验,杂志文章


    authors: Gatzen C,Scheltinga MR,Kimbrough TD,Jacobs DO,Wilmore DW

    更新日期:1992-08-01 00:00:00

  • Long-term outcomes of laparoscopic versus open liver resection for liver metastases from colorectal cancer: A comparative analysis of 168 consecutive cases at a single center.

    abstract:BACKGROUND:Laparoscopic liver resection for liver metastases from colorectal cancer (CRLM) is performed in a relatively small number of institutions. Its operative results have been reported to be comparable with that of open laparotomy; however, information on its oncologic outcomes is scarce. This study aimed to comp...


    pub_type: 杂志文章


    authors: Hasegawa Y,Nitta H,Sasaki A,Takahara T,Itabashi H,Katagiri H,Otsuka K,Nishizuka S,Wakabayashi G

    更新日期:2015-06-01 00:00:00

  • Vascular complications associated with pancreaticoduodenectomy adversely affect clinical outcome.

    abstract:BACKGROUND:Early survival after pancreaticoduodenectomy has improved, but its morbidity remains high. The purpose of this study is to determine how the intra-operative (OR) occurrence of major vascular complications affects the outcome of pancreaticoduodenectomy. METHODS:The medical records of 180 consecutive patients...


    pub_type: 杂志文章


    authors: Kim AW,McCarthy WJ 3rd,Maxhimer JB,Quiros RM,Hollinger EF,Doolas A,Millikan KW,Deziel DJ,Godellas CV,Prinz RA

    更新日期:2002-10-01 00:00:00

  • Quality of post-treatment surveillance of early stage breast cancer in Texas.

    abstract:BACKGROUND:Only annual mammography and physical examination are recommended for the post-treatment surveillance of early stage breast cancer. METHODS:We used Texas Cancer Registry-Medicare linked data (2001-2007) to identify physician visits and use of mammography, magnetic resonance imaging (MRI), computed tomography...


    pub_type: 杂志文章


    authors: Parmar AD,Sheffield KM,Vargas GM,Han Y,Chao C,Riall TS

    更新日期:2013-08-01 00:00:00

  • Stress-induced fractal rearrangement of the endothelial cell cytoskeleton causes apoptosis.

    abstract:BACKGROUND:Apoptosis, a mechanism of cell death prominent in critical illnesses including disseminated inflammation and multiorgan dysfunction syndrome, is characterized by morphologic changes including cell shrinkage, condensation of organelles, blebbing, and chromatin fragmentation. These phenomena suggest substantia...


    pub_type: 杂志文章


    authors: DeMeester SL,Cobb JP,Hotchkiss RS,Osborne DF,Karl IE,Tinsley KW,Buchman TG

    更新日期:1998-08-01 00:00:00

  • Effects of intraoperative chemohyperthermia in patients with gastric cancer with peritoneal dissemination.

    abstract:BACKGROUND:The most common cause of noncurative resection and recurrence is gastric cancer is peritoneal seeding. However, the results of treatment of peritoneal dissemination with chemotherapy have been poor with 5-year survival rates of 0%. METHODS:A new in vitro thermochemosensitivity test was performed on gastric ...


    pub_type: 杂志文章


    authors: Yonemura Y,Fujimura T,Nishimura G,FallaR,Sawa T,Katayama K,Tsugawa K,Fushida S,Miyazaki I,Tanaka M,Endou Y,Sasaki T

    更新日期:1996-04-01 00:00:00

  • Preoperative immunonutrition decreases postoperative complications by modulating prostaglandin E2 production and T-cell differentiation in patients undergoing pancreatoduodenectomy.

    abstract:BACKGROUND:An immune-enhancing diet has been used to alter eicosanoid synthesis, cytokine production, and immune function in an attempt to limit the undesired immune reactions after injury from surgery. This prospective randomized study was designed to investigate the effect of preoperative immunonutrition on operative...


    pub_type: 杂志文章,随机对照试验


    authors: Aida T,Furukawa K,Suzuki D,Shimizu H,Yoshidome H,Ohtsuka M,Kato A,Yoshitomi H,Miyazaki M

    更新日期:2014-01-01 00:00:00

  • Designing a proficiency-based, content validated virtual reality curriculum for laparoscopic colorectal surgery: a Delphi approach.

    abstract:BACKGROUND:Although task training on virtual reality (VR) simulators has been shown to transfer to the operating room, to date no VR curricula have been described for advanced laparoscopic procedures. The purpose of this study was to develop a proficiency-based VR technical skills curriculum for laparoscopic colorectal...


    pub_type: 杂志文章


    authors: Palter VN,Graafland M,Schijven MP,Grantcharov TP

    更新日期:2012-03-01 00:00:00

  • Effects of perfluorocarbon exchange transfusion on reducing myocardial infarct size in a primate model of ischemia-reperfusion injury: a prospective, randomized study.

    abstract:BACKGROUND:This study was undertaken to test the hypothesis that perfluorocarbons were able to reduce myocardial infarct size in a baboon model of ischemia-reperfusion injury. Exchange transfusion of perfluorocarbons has been shown to reduce myocardial infarct size in the dog, who, unlike the baboon, has an extensive c...


    pub_type: 杂志文章


    authors: Premaratne S,Harada RN,Chun P,Suehiro A,McNamara JJ

    更新日期:1995-06-01 00:00:00

  • Spontaneous and polyclonal Ig secretion by circulating B cells after surgery.

    abstract::Abnormalities of the immune response are commonly observed after surgery. In many cases, they are part of a physiologic rather than of a pathologic response to trauma. In this study we show that after elective surgery in otherwise healthy subjects the B cell compartment is deeply affected, as documented by the appeara...


    pub_type: 杂志文章


    authors: Di Padova F,Durig M,Di Padova C,Pozzoli M,Tritapepe R

    更新日期:1988-05-01 00:00:00

  • Inhibition of factor Xa suppresses the expression of tissue factor in human monocytes and lipopolysaccharide-induced endotoxemia in rats.

    abstract:BACKGROUND:Activated factor X (FXa) is involved in hemostasis, thrombogenesis, inflammation, and cellular immune response. Tissue factor (TF) is an initiator of blood coagulation. We investigated whether FXa induces TF expression in human peripheral monocytes and whether treatment with FXa inhibitor reduces TF expressi...


    pub_type: 杂志文章


    authors: Akahane K,Okamoto K,Kikuchi M,Todoroki H,Higure A,Ohuchida T,Kitahara K,Takeda S,Itoh H,Ohsato K

    更新日期:2001-11-01 00:00:00

  • Sepsis and endotoxemia stimulate intestinal interleukin-6 production.

    abstract:BACKGROUND:Endotoxemia stimulates tumor necrosis factor (TNF) and interleukin-1 (IL-1) production in mucosa of the small intestine, but the effect on IL-6 production is not known. Intestinal IL-6 may be especially important, considering its role in the acute phase response. We tested the influence of endotoxemia and se...


    pub_type: 杂志文章


    authors: Meyer TA,Wang J,Tiao GM,Ogle CK,Fischer JE,Hasselgren PO

    更新日期:1995-08-01 00:00:00

  • Influence of surgical margin on type of recurrence after liver resection for colorectal metastases: a single-center experience.

    abstract:BACKGROUND:Hepatectomy for colorectal liver metastases (CRLM) may offer good long-term survival. The impact of the tumor-free surgical margin on long-term results remains controversial, and we have assessed this component in 185 patients. METHODS:Between 1992 and 2005, 185 patients underwent primary hepatectomy with c...


    pub_type: 杂志文章


    authors: Nuzzo G,Giuliante F,Ardito F,Vellone M,Giovannini I,Federico B,Vecchio FM

    更新日期:2008-03-01 00:00:00

  • Novel approach to iatrogenic bile peritonitis.

    abstract::Bile peritonitis after injury to the biliary tree is a serious complication that requires exploratory laparotomy. Our patient had an obstructing ampullary carcinoma, and generalized bile peritonitis developed from attempted percutaneous transhepatic cholangiography. The patient's condition was managed by peritoneal la...


    pub_type: 杂志文章


    authors: McAneny DB,Gibran NS,Braasch JW

    更新日期:1991-06-01 00:00:00

  • A crucial effect of splenectomy on prolonging cardiac xenograft survival in combination with cyclosporine.

    abstract::In this study we investigated the effect of splenectomy in combination with cyclosporine (CsA) on survival of heterotopic cardiac xenografts from hamster to rat. A 12-fold prolongation of mean cardiac xenograft survival, to 41 days, was accomplished with the combined therapy. In both untreated controls and CsA-treated...


    pub_type: 杂志文章


    authors: Monden M,Valdivia LA,Gotoh M,Kubota N,Hasuike Y,Nakano Y,Okamura J,Mori T

    更新日期:1989-04-01 00:00:00

  • Recovery of liver function in two-third partial hepatectomized rats evaluated by L-[1-13C]phenylalanine breath test.

    abstract:BACKGROUND:We have previously reported that by means of a breath test with intravenously administered L-[1-13C] phenylalanine (13Cphe), hepatopathy could be quantitatively evaluated by measuring expiratory 13CO2 levels in a short period. It is known that phenylalanine hydroxylase activity (PAHA) plays an important role...


    pub_type: 杂志文章


    authors: Ishii Y,Asai S,Kohno T,Ito A,Iwai S,Ishikawa K

    更新日期:2002-11-01 00:00:00

  • Caudate lobe resection to reduce inferior vena caval hypertension prior to portacaval shunt.

    abstract::Coexistent caval and portal hypertension was recognized on preoperative angiographic study of a patient with cirrhosis and variceal bleeding. This hemodynamic situation can preclude adequate reduction of portal hypertension by portasystemic shunt and can result in fatal postoperative bleeding. Resection of a very larg...


    pub_type: 杂志文章


    authors: Glotzer DJ,Martini DJ,Sacks BA

    更新日期:1980-05-01 00:00:00

  • The System for Telementoring with Augmented Reality (STAR): A head-mounted display to improve surgical coaching and confidence in remote areas.

    abstract:BACKGROUND:The surgical workforce particularly in rural regions needs novel approaches to reinforce the skills and confidence of health practitioners. Although conventional telementoring systems have proven beneficial to address this gap, the benefits of platforms of augmented reality-based telementoring in the coachin...


    pub_type: 杂志文章


    authors: Rojas-Muñoz E,Cabrera ME,Lin C,Andersen D,Popescu V,Anderson K,Zarzaur BL,Mullis B,Wachs JP

    更新日期:2020-04-01 00:00:00

  • Ethanol impairs intestinal barrier defense by modulation of immunoglobulin A transport.

    abstract:BACKGROUND:Increased epithelial permeability may contribute to septic complications after alcohol intoxication. Prior ethanol (EtOH) exposure leads to structural and functional effects on cytoskeletal components important in maintaining barrier integrity. The cytoskeleton is also important in the transcellular transpor...


    pub_type: 杂志文章


    authors: Diebel LN,Liberati DM,Dulchavsky SA,Diglio CA,Brown WJ

    更新日期:2002-10-01 00:00:00

  • Proteomics-based analysis of lung injury-induced proteins in a mouse model of common bile duct ligation.

    abstract:BACKGROUND:Lung injury is a life-threatening complication in patients with liver dysfunction. We recently provided an experimental lung injury model in mouse with common bile duct ligation. In this study, we aimed to characterize the pathologic and biochemical features of lung tissues in common bile duct ligation mice ...


    pub_type: 杂志文章


    authors: Sakaue T,Shikata F,Utsunomiya K,Fukae S,Kurata M,Nakaoka H,Okazaki M,Kawanishi Y,Kojima A,Higashiyama S,Izutani H

    更新日期:2017-06-01 00:00:00

  • Therapeutic efficacy of Biobrane in partial- and full-thickness thermal injury.

    abstract::Cadaver allograft skin, porcine xenograft skin, and amniotic membranes have been proved to be adequate temporary thermal wound coverings in four clinical situations: coverage of shallow wounds while awaiting epithelialization (SW), coverage of deep wounds after eschar excision (DEW), coverage of widely meshed autograf...


    pub_type: 杂志文章


    authors: McHugh TP,Robson MC,Heggers JP,Phillips LG,Smith DJ Jr,McCollum MC

    更新日期:1986-10-01 00:00:00

  • DTIC therapy in patients with malignant intra-abdominal neuroendocrine tumors.

    abstract::Malignant intra-abdominal neuroendocrine tumors are rare; consequently, a standard chemotherapeutic protocol for patients with unresectable disease has not been established. This prompted a review of our experience with dimethyltriazeno imidazole carboxamide (dacarbazine) (DTIC) treatment for these tumors. From 1976 t...


    pub_type: 杂志文章


    authors: Altimari AF,Badrinath K,Reisel HJ,Prinz RA

    更新日期:1987-12-01 00:00:00