Myeloperoxidase gene expression in blast cells with a lymphoid phenotype in cases of acute lymphoblastic leukemia.


:By using a cDNA clone of the myeloperoxidase (MPO) gene, we have studied, by Northern blot analysis, the level of MPO mRNA in eight cases of acute lymphoblastic leukemia (ALL). The blast cell populations studied were characterized by morphologic, cytochemical, immunochemical, and molecular criteria. With all the methods used the populations were found to be highly homogeneous and showed a typical lymphoid phenotype. In particular, the Ig heavy-chain gene rearrangement was largely prevalent, and the germ line configuration was almost absent. However, in three of eight cases, high levels of MPO mRNA were detected. The remarkable homogeneity of the cell populations examined suggests that the MPO mRNA observed was present in cellular elements certainly identified as lymphoid. The absence of contamination by myeloid cells was confirmed by the results of Western blot analysis of the proteins of the cell population studied: no MPO protein was detectable. The levels of mRNA observed were high enough to be comparable to those observed in a promyelocytic cell population.






Ferrari S,Mariano MT,Tagliafico E,Sarti M,Ceccherelli G,Selleri L,Merli F,Narni F,Donelli A,Torelli G


Has Abstract,Author List Incomplete


1988-09-01 00:00:00














  • Activity of transgene-produced B-domain-deleted factor VIII in human plasma following AAV5 gene therapy.

    abstract::Adeno-associated virus (AAV)-based gene therapies can restore endogenous factor VIII (FVIII) expression in hemophilia A (HA). AAV vectors typically use a B-domain-deleted FVIII transgene, such as human FVIII-SQ in valoctocogene roxaparvovec (AAV5-FVIII-SQ). Surprisingly, the activity of transgene-produced FVIII-SQ was...


    pub_type: 杂志文章


    authors: Rosen S,Tiefenbacher S,Robinson M,Huang M,Srimani J,Mackenzie D,Christianson T,Pasi KJ,Rangarajan S,Symington E,Giermasz A,Pierce GF,Kim B,Zoog SJ,Vettermann C

    更新日期:2020-11-26 00:00:00

  • Amelioration of Sardinian beta0 thalassemia by genetic modifiers.

    abstract::Sardinian beta-thalassemia patients all are homozygotes for the same null allele in the beta-globin gene, but the clinical manifestations are extremely variable in severity. Previous studies have shown that the coinheritance of alpha-thalassemia or the presence of genetic variants that sustain fetal hemoglobin product...


    pub_type: 杂志文章


    authors: Galanello R,Sanna S,Perseu L,Sollaino MC,Satta S,Lai ME,Barella S,Uda M,Usala G,Abecasis GR,Cao A

    更新日期:2009-10-29 00:00:00

  • Cyclophosphamide enhances immunity by modulating the balance of dendritic cell subsets in lymphoid organs.

    abstract::Cyclophosphamide (CTX), a commonly used chemotherapeutic agent can enhance immune responses. The ability of CTX to promote the proliferation of effector T cells and abrogate the function of regulatory T cells (Tregs) has been described. In this study, we examined the effects of CTX treatment on dendritic cell (DC) sub...


    pub_type: 杂志文章


    authors: Nakahara T,Uchi H,Lesokhin AM,Avogadri F,Rizzuto GA,Hirschhorn-Cymerman D,Panageas KS,Merghoub T,Wolchok JD,Houghton AN

    更新日期:2010-06-03 00:00:00

  • Gene expression analysis uncovers similarity and differences among Burkitt lymphoma subtypes.

    abstract::Burkitt lymphoma (BL) is classified into 3 clinical subsets: endemic, sporadic, and immunodeficiency-associated BL. So far, possible differences in their gene expression profiles (GEPs) have not been investigated. We studied GEPs of BL subtypes, other B-cell lymphomas, and B lymphocytes; first, we found that BL is a u...


    pub_type: 杂志文章


    authors: Piccaluga PP,De Falco G,Kustagi M,Gazzola A,Agostinelli C,Tripodo C,Leucci E,Onnis A,Astolfi A,Sapienza MR,Bellan C,Lazzi S,Tumwine L,Mawanda M,Ogwang M,Calbi V,Formica S,Califano A,Pileri SA,Leoncini L

    更新日期:2011-03-31 00:00:00

  • Hyaluronate-CD44 interactions can induce murine B-cell activation.

    abstract::CD44 is a widely distributed cell surface glycoprotein whose principal ligand has been identified as hyaluronic acid (HA), a major component of the extracellular matrix (ECM). Recent studies have demonstrated that activation through CD44 leads to induction of effector function in T cells and macrophages. In the curren...


    pub_type: 杂志文章


    authors: Rafi A,Nagarkatti M,Nagarkatti PS

    更新日期:1997-04-15 00:00:00

  • Platelets: connecting clotting and lysis.

    abstract::In this issue of Blood, Whyte et al describe how under flow conditions, phosphatidylserine-expressing platelets modulate the lysis of whole blood clots by providing direct and indirect binding sites for plasminogen. ...


    pub_type: 评论,杂志文章


    authors: Kim PY

    更新日期:2015-04-16 00:00:00

  • Donor origin of multipotent adult progenitor cells in radiation chimeras.

    abstract::Multipotent adult progenitor cells (MAPCs) are bone marrow-derived stem cells that have extensive in vitro expansion capacity and can differentiate in vivo and in vitro into tissue cells of all 3 germinal layers: ectoderm, mesoderm, and endoderm. The origin of MAPCs within bone marrow is unknown. MAPCs are believed to...


    pub_type: 杂志文章


    authors: Reyes M,Li S,Foraker J,Kimura E,Chamberlain JS

    更新日期:2005-11-15 00:00:00

  • Intermediate-dose versus high-dose prophylaxis for severe hemophilia: comparing outcome and costs since the 1970s.

    abstract::Prophylactic treatment in severe hemophilia is very effective but is limited by cost issues. The implementation of 2 different prophylactic regimens in The Netherlands and Sweden since the 1970s may be considered a natural experiment. We compared the costs and outcomes of Dutch intermediate- and Swedish high-dose prop...


    pub_type: 杂志文章


    authors: Fischer K,Steen Carlsson K,Petrini P,Holmström M,Ljung R,van den Berg HM,Berntorp E

    更新日期:2013-08-15 00:00:00

  • Long-awaited news for hepatic veno-occlusive disease.

    abstract::In this issue of Blood, Palomo et al provide new insight into the mechanism of action of defibrotide as an endothelial protective agent, while Richardson et al present the encouraging final results of the phase 3 clinical study of defibrotide for the treatment of severe hepatic veno-occlusive disease (VOD), showing a ...


    pub_type: 评论,杂志文章


    authors: Arai S

    更新日期:2016-03-31 00:00:00

  • Gait speed, grip strength, and clinical outcomes in older patients with hematologic malignancies.

    abstract::This study aimed to evaluate whether gait speed and grip strength predicted clinical outcomes among older adults with blood cancers. We prospectively recruited 448 patients aged 75 years and older presenting for initial consultation at the myelodysplastic syndrome/leukemia, myeloma, or lymphoma clinic of a large terti...


    pub_type: 杂志文章


    authors: Liu MA,DuMontier C,Murillo A,Hshieh TT,Bean JF,Soiffer RJ,Stone RM,Abel GA,Driver JA

    更新日期:2019-07-25 00:00:00

  • Transformation of Fanconi's anemia to acute nonlymphocytic leukemia associated with emergence of monosomy 7.

    abstract::Two sisters in whom a diagnosis of Fanconi's anemia was made at ages 12 and 18 subsequently developed acute nonlymphocytic leukemia (ANLL). A third sibling had previously died at age 11 of apparent sepsis. Both sisters had cytogenetic studies that showed increased chromosomal breakage and a 46,XX karyotype, but subseq...


    pub_type: 杂志文章


    authors: Stivrins TJ,Davis RB,Sanger W,Fritz J,Purtilo DT

    更新日期:1984-07-01 00:00:00

  • Vascular endothelial growth factor inhibits the development of dendritic cells and dramatically affects the differentiation of multiple hematopoietic lineages in vivo.

    abstract::Defective function of dendritic cells (DC) in cancer has been recently described and may represent one of the mechanisms of tumor evasion from immune system control. We have previously shown in vitro that vascular endothelial growth factor (VEGF), produced by almost all tumors, is one of the tumor-derived factors resp...


    pub_type: 杂志文章


    authors: Gabrilovich D,Ishida T,Oyama T,Ran S,Kravtsov V,Nadaf S,Carbone DP

    更新日期:1998-12-01 00:00:00

  • The molecular species composition of phosphatidylcholine affects cellular properties in normal and sickle erythrocytes.

    abstract::The phosphatidylcholine specific transfer protein (PCTP) from bovine liver was used to retailor the molecular species composition of phosphatidylcholine (PC) in the membrane of normal (AA) and sickleable (SS) human erythrocytes. Changes in molecular species composition of PC altered morphology as well as cellular defo...


    pub_type: 杂志文章


    authors: Kuypers FA,Chiu D,Mohandas N,Roelofsen B,Op den Kamp JA,Lubin B

    更新日期:1987-10-01 00:00:00

  • Mechanisms of anti-GPIbα antibody-induced thrombocytopenia in mice.

    abstract::Immune thrombocytopenia (ITP) is an acquired bleeding disorder characterized by antibody-mediated platelet destruction. Different mechanisms have been suggested to explain accelerated platelet clearance and impaired thrombopoiesis, but the pathophysiology of ITP has yet to be fully delineated. In this study, we tested...


    pub_type: 杂志文章


    authors: Morodomi Y,Kanaji S,Won E,Ruggeri ZM,Kanaji T

    更新日期:2020-06-18 00:00:00

  • Resistance to hepcidin is conferred by hemochromatosis-associated mutations of ferroportin.

    abstract::Ferroportin (FPN) mediates iron export from cells; FPN mutations are associated with the iron overloading disorder hemochromatosis. Previously, we found that the A77D, V162del, and G490D mutations inhibited FPN activity, but that other disease-associated FPN variants retained full iron export capability. The peptide h...


    pub_type: 杂志文章


    authors: Drakesmith H,Schimanski LM,Ormerod E,Merryweather-Clarke AT,Viprakasit V,Edwards JP,Sweetland E,Bastin JM,Cowley D,Chinthammitr Y,Robson KJ,Townsend AR

    更新日期:2005-08-01 00:00:00

  • Role of the sialophorin (CD43) receptor in mediating influenza A virus-induced polymorphonuclear leukocyte dysfunction.

    abstract::Polymorphonuclear leukocytes (PMNLs) exposed to influenza A virus (IAV) undergo activation of the respiratory burst followed by depression of cell function when subsequently exposed to particulate or soluble stimuli. The effect of IAV on PMNLs is likely to be mediated through the attachment of IAV to one or more speci...


    pub_type: 杂志文章


    authors: Abramson JS,Hudnor HR

    更新日期:1995-03-15 00:00:00

  • Clinical significance of HOX11L2 expression linked to t(5;14)(q35;q32), of HOX11 expression, and of SIL-TAL fusion in childhood T-cell malignancies: results of EORTC studies 58881 and 58951.

    abstract::In a series of 153 children with T-cell malignancies enrolled in 2 consecutive European Organization for Research and Treatment of Cancer (EORTC) trials, we assessed the HOX11L2 expression and/or the presence of a t(5;14)(q35;q32). Additionally, in 138 of these patients, HOX11 expression and SIL-TAL rearrangement were...


    pub_type: 杂志文章,多中心研究


    authors: Cavé H,Suciu S,Preudhomme C,Poppe B,Robert A,Uyttebroeck A,Malet M,Boutard P,Benoit Y,Mauvieux L,Lutz P,Méchinaud F,Grardel N,Mazingue F,Dupont M,Margueritte G,Pages MP,Bertrand Y,Plouvier E,Brunie G,Bastard C,P

    更新日期:2004-01-15 00:00:00

  • Anti-D initially stimulates an Fc-dependent leukocyte oxidative burst and subsequently suppresses erythrophagocytosis via interleukin-1 receptor antagonist.

    abstract::Previous results have demonstrated that anti-D therapy in children with chronic auto-immune thrombocytopenic purpura (AITP) induced a significant increase in several pro- and anti-inflammatory plasma cytokines within 2 hours of administration. To investigate the biologic basis of these early in vivo responses, we deve...


    pub_type: 杂志文章


    authors: Coopamah MD,Freedman J,Semple JW

    更新日期:2003-10-15 00:00:00

  • In vitro-differentiated T/natural killer-cell progenitors derived from human CD34+ cells mature in the thymus.

    abstract::Haploidentical hematopoietic stem cell transplantation (haplo-HSCT) is a treatment option for patients with hematopoietic malignancies that is hampered by treatment-related morbidity and mortality, in part the result of opportunistic infections, a direct consequence of delayed T-cell recovery. Thymic output can be imp...


    pub_type: 杂志文章


    authors: Meek B,Cloosen S,Borsotti C,Van Elssen CH,Vanderlocht J,Schnijderberg MC,van der Poel MW,Leewis B,Hesselink R,Manz MG,Katsura Y,Kawamoto H,Germeraad WT,Bos GM

    更新日期:2010-01-14 00:00:00

  • Characterization of the T-cell repertoire in autologous graft-versus-host disease (GVHD): evidence for the involvement of antigen-driven T-cell response in the development of autologous GVHD.

    abstract::Administration of cyclosporine A (CsA) after autologous stem cell transplantation elicits an autoimmune syndrome with pathology similar to graft-versus-host disease (GVHD). This syndrome, termed autologous GVHD, is associated with the appearance of autoreactive T cells directed at major histocompatibility class (MHC) ...


    pub_type: 杂志文章


    authors: Miura Y,Thoburn CJ,Bright EC,Sommer M,Lefell S,Ueda M,Nakao S,Hess AD

    更新日期:2001-08-01 00:00:00

  • Achievement of VGPR to induction therapy is an important prognostic factor for longer PFS in the IFM 2005-01 trial.

    abstract::In the 2005-01 trial, we have demonstrated that bortezomib-dexamethasone as induction therapy before autologous stem cell transplantation was superior to vincristine-adriamycin-dexamethasone. We conducted a post-hoc analysis to assess the prognostic impact of initial characteristics as well as response to therapy in p...


    pub_type: 杂志文章,随机对照试验


    authors: Moreau P,Attal M,Pégourié B,Planche L,Hulin C,Facon T,Stoppa AM,Fuzibet JG,Grosbois B,Doyen C,Ketterer N,Sebban C,Kolb B,Chaleteix C,Dib M,Voillat L,Fontan J,Garderet L,Jaubert J,Mathiot C,Esseltine D,Avet-Loise

    更新日期:2011-03-17 00:00:00

  • FCR achieves long-term durable remissions in patients with IGHV-mutated CLL.

    abstract::In chronic lymphocytic leukemia (CLL) patients with mutated IGHV, 3 recent studies have demonstrated prolonged progression-free survival (PFS) after treatment with fludarabine-cyclophosphamide-rituximab (FCR) chemoimmunotherapy. We performed a systematic review to assess the benefit of FCR for patients with CLL and id...


    pub_type: 杂志文章,评审


    authors: Chai-Adisaksopha C,Brown JR

    更新日期:2017-11-23 00:00:00

  • Rapid single-molecule digital detection of protein biomarkers for continuous monitoring of systemic immune disorders.

    abstract::Digital protein assays have great potential to advance immunodiagnostics because of their single-molecule sensitivity, high precision, and robust measurements. However, translating digital protein assays to acute clinical care has been challenging because it requires their deployment with a rapid turnaround. Herein, w...


    pub_type: 杂志文章


    authors: Song Y,Sandford E,Tian Y,Yin Q,Kozminski AG,Su SH,Cai T,Ye Y,Chung MT,Lindstrom R,Goicochea A,Barabas J,Olesnavich M,Rozwadowski M,Li Y,Alam HB,Singer BH,Ghosh M,Choi SW,Tewari M,Kurabayashi K

    更新日期:2020-12-04 00:00:00

  • Recurrent acute splenic sequestration crisis due to interacting genetic defects: hemoglobin SC disease and hereditary spherocytosis.

    abstract::A 14-year-old boy with hemoglobin SC disease and alpha-thalassemia-2 experienced five episodes of acute splenic sequestration crisis (ASSC), while two of his siblings with identical globin genotypes (SC and -alpha/alpha alpha) had no such experience. To determine if an additional red blood cell (RBC) defect was respon...


    pub_type: 杂志文章


    authors: Warkentin TE,Barr RD,Ali MA,Mohandas N

    更新日期:1990-01-01 00:00:00

  • Rapid and efficient homing of human CD34(+)CD38(-/low)CXCR4(+) stem and progenitor cells to the bone marrow and spleen of NOD/SCID and NOD/SCID/B2m(null) mice.

    abstract::Stem cell homing into the bone microenvironment is the first step in the initiation of marrow-derived blood cells. It is reported that human severe combined immunodeficient (SCID) repopulating cells home and accumulate rapidly, within a few hours, in the bone marrow and spleen of immunodeficient mice previously condit...


    pub_type: 杂志文章


    authors: Kollet O,Spiegel A,Peled A,Petit I,Byk T,Hershkoviz R,Guetta E,Barkai G,Nagler A,Lapidot T

    更新日期:2001-05-15 00:00:00

  • Stat3 signaling in acute myeloid leukemia: ligand-dependent and -independent activation and induction of apoptosis by a novel small-molecule Stat3 inhibitor.

    abstract::Acute myeloid leukemia (AML) is an aggressive malignancy with a relapse rate approaching 50%, despite aggressive chemotherapy. New therapies for AML are targeted at signal transduction pathways known to support blast survival, such as the Stat3 pathway. Aberrant activation of Stat3 has been demonstrated in many differ...


    pub_type: 杂志文章


    authors: Redell MS,Ruiz MJ,Alonzo TA,Gerbing RB,Tweardy DJ

    更新日期:2011-05-26 00:00:00

  • Macrophage mannose receptor on lymphatics controls cell trafficking.

    abstract::Macrophage mannose receptor (MR) participates in pathogen recognition, clearance of endogenous serum glycoproteins, and antigen presentation. MR is also present on lymphatic vessels, where its function is unknown. Here we show that migration of lymphocytes from the skin into the draining lymph nodes through the affere...


    pub_type: 杂志文章


    authors: Marttila-Ichihara F,Turja R,Miiluniemi M,Karikoski M,Maksimow M,Niemelä J,Martinez-Pomares L,Salmi M,Jalkanen S

    更新日期:2008-07-01 00:00:00

  • Monoclonal Lym-1 antibody-dependent lysis of B-lymphoblastoid tumor targets by human complement and cytokinine-exposed mononuclear and neutrophilic polymorphonuclear leukocytes.

    abstract::Lym-1 is a murine IgG2a monoclonal antibody that recognizes a polymorphic variant of HLA-DR antigens on malignant B cells, with minimal cross-reactivity with normal tissues. Because it can be safely administered in vivo, a detailed knowledge of its ability to recruit and trigger the antitumor immune effector systems i...


    pub_type: 杂志文章


    authors: Ottonello L,Morone P,Dapino P,Dallegri F

    更新日期:1996-06-15 00:00:00

  • Spatial localization of transplanted hemopoietic stem cells: inferences for the localization of stem cell niches.

    abstract::The spatial distribution of subpopulations of hemopoietic progenitor cells following syngeneic transplantation was investigated at the single-cell level. The location of infused hemopoietic progenitor cells within the femoral bone marrow of nonablated recipients was determined by 5-(and-6)-carboxyfluorescein diacetate...


    pub_type: 杂志文章


    authors: Nilsson SK,Johnston HM,Coverdale JA

    更新日期:2001-04-15 00:00:00

  • X chromosome inactivation analysis reveals a difference in the biology of ET patients with JAK2 and CALR mutations.

    abstract::Calreticulin mutations (CALR(MUT)) are found in a significant proportion of patients with essential thrombocythemia (ET) lacking JAK2(V617F) or MPL mutations. They are associated with substantially different hematological and clinical features and define a distinct subtype of ET. We show here that their presence is si...


    pub_type: 杂志文章


    authors: Allen C,Lambert JR,Linch DC,Gale RE

    更新日期:2014-09-25 00:00:00