The recombination activation gene 1 (RAG1) of rainbow trout (Oncorhynchus mykiss): cloning, expression, and phylogenetic analysis.


:The characterization of genes involved in the generation of the immune repertoire is an active area of research in lower vertebrate taxa. The recombination activating genes (RAG) have been shown to be essential for V (D) J recombination of T-cell antigen receptor (TCR) and immunoglobulin (Ig) genes, leading to the generation of the primary repertoire. As RAG1 is critical to the differentiation of pre-B and -T cells, its expression within an associated primary lymphoid organ can serve as a developmental marker. To examine the ontogeny of lymphocytes in Oncorhynchus mykiss, we cloned RAG1 from trout and examined its tissue- and lymphocyte-specific expression. The polymerase chain reaction, coupled with degenerate oligonucleotide primers, was used to amplify a homologous probe [(633 base pairs) (bp)] from rainbow trout genomic DNA, which in turn was used to isolate a lambda genomic clone. Sequence analysis of this genomic clone confirmed the RAG1 nature of this gene (3888 bp) and revealed an internal intron of 666 bp. When compared with other previously reported RAG1 sequences, the predicted amino acid translation (1073 aa) displayed a minimum of 78% similarity for the complete sequence and 89% similarity in the conserved region (aa 417-1042). Using northern blot analysis, we found the expression of RAG1 to be limited to surface Ig-n lymphocytes within the thymus. This data forms the basis for a proposal that the thymus of teleost species plays an essential developmental role in lymphopoiesis and thus can be regarded as a primary lymphoid organ.






Hansen JD,Kaattari SL




Has Abstract


1995-01-01 00:00:00












  • SNEP: SNP-derived epitope prediction program for minor H antigens.

    abstract::The single nucleotide polymorphism (SNP)-derived Epitope Prediction program (SNEP) is now available to the public. It predicts minor histocompatibility antigens (miHAgs), which are T-cell epitopes containing polymorphic spots, from proteins listed in the SWISS-PROT database. SNEP recognizes polymorphisms (termed VARIA...


    pub_type: 杂志文章


    authors: Schuler MM,Dönnes P,Nastke MD,Kohlbacher O,Rammensee HG,Stevanovic S

    更新日期:2005-12-01 00:00:00

  • Independent expression of the two paralogous CCL4 genes in monocytes and B lymphocytes.

    abstract::The CCL4 chemokine is secreted by a variety of cells following stimulation. CCL4 affects several different types of cells that are important for acute inflammatory responses and are critical for the development of specific immune responses to foreign antigens. The human genome contains two genes for the CCL4 chemokine...


    pub_type: 杂志文章


    authors: Lu J,Honczarenko M,Sloan SR

    更新日期:2004-01-01 00:00:00

  • Localization of Idd11 using NOD congenic mouse strains: elimination of Slc9a1 as a candidate gene.

    abstract::Type 1 diabetes is a multigenic autoimmune disease, the genetic basis for which is perhaps best characterized in the nonobese diabetic (NOD) mouse model. We previously located a NOD diabetes susceptibility locus, designated Idd11, on mouse Chromosome (Chr) 4 by analyzing diabetic backcross mice produced after crossing...


    pub_type: 杂志文章


    authors: Brodnicki TC,McClive P,Couper S,Morahan G

    更新日期:2000-01-01 00:00:00

  • Characterization of MHC class I in a long-distance migrant shorebird suggests multiple transcribed genes and intergenic recombination.

    abstract::The major histocompatibility complex (MHC) includes highly polymorphic gene families encoding proteins crucial to the vertebrate acquired immune system. Classical MHC class I (MHCI) genes code for molecules expressed on the surfaces of most nucleated cells and are associated with defense against intracellular pathogen...


    pub_type: 杂志文章


    authors: Buehler DM,Verkuil YI,Tavares ES,Baker AJ

    更新日期:2013-03-01 00:00:00

  • Gm and Km immunoglobulin allotypes in Sicily.

    abstract::The aim of this study was to evaluate the intra- and inter-population variability of the Gm/Km system in the Madonie Mountains, one of the main geographical barriers in north-central Sicily. We analysed 392 samples: 145 from Alia, 128 from Valledolmo, 25 from Cerda and 94 from Palermo. Serum samples were tested for G1...


    pub_type: 历史文章,杂志文章


    authors: Cerutti N,Dugoujon JM,Guitard E,Rabino Massa E

    更新日期:2004-01-01 00:00:00

  • Lyb-8.2: A new B cell antigen defined and characterized with a monoclonal antibody.

    abstract::A DBA/1 B10.D2-specific monoclonal antibody (CY34) is described which defines a new murine B lymphocyte differentiation antigen designated Lyb-8.2. The ontogeny, strain distribution, and cell-surface density of the antigen were studied by radioimmunoassay and by fluorescence-activated cell sorter (FACS) analysis. Lyb-...


    pub_type: 杂志文章


    authors: Symington FW,Subbarao B,Mosier DE,Sprent J

    更新日期:1982-01-01 00:00:00

  • Analysis of the sheep MHC using HLA class I, II, and C4 cDNA probes.

    abstract::Four cDNA probes for the human major histocompatibility complex (MHC) were used to investigate the sheep MHC, in conjunction with serological typing for ovine lymphocyte antigen (OLA). Lymphocytes from a family (two parents and five offspring) of Romanov sheep were subjected to genomic DNA digestion by the restriction...


    pub_type: 杂志文章


    authors: Chardon P,Kirszenbaum M,Cullen PR,Geffrotin C,Auffray C,Strominger JL,Cohen D,Vaiman M

    更新日期:1985-01-01 00:00:00

  • H-2 antigens as genetic markers: a two-dimensional gel electrophoretic study.

    abstract::Direct immunoprecipitation and two-dimensional (2D) gel electrophoresis have been used to identify and characterize genetic variation of the H-2K and H-2D regions. Using inbred strains of mice and alloantisera, haplotype-specific polypeptides were defined for five different H-2 haplotypes. Specific immunoprecipitates ...


    pub_type: 杂志文章


    authors: Sawicki JA,Epstein CJ

    更新日期:1981-01-01 00:00:00

  • Analysis of three distinct Ly6-A-related cDNA sequences isolated from rat kidney.

    abstract::Mouse Ly6A and Ly6C cDNA probes were hybridized to total RNA of rat tissues and, as in mouse, the highest level of Ly6-related transcripts was detected in kidney. Therefore, Ly6-related cDNA clones were isolated from a commercial rat kidney cDNA library in lambda gt11. Four of these (RK3, RK6, RK10, and RK11) have bee...


    pub_type: 杂志文章


    authors: Friedman S,Palfree RG,Sirlin S,Hämmerling U

    更新日期:1990-01-01 00:00:00

  • Identification and characterization of the lamprey cathepsin genes.

    abstract::Cathepsins are key mammalian proteases that play an important role in the immune response. Several studies have revealed the versatile and critical functions of cathepsins. Here, we obtained ten kinds of cathepsin homologs and identified seven homologs with complete coding sequences. Phylogenetic analysis verified the...


    pub_type: 杂志文章


    authors: Wang D,Su P,Wang X,Liu K,Li C,Gao X,Lu J,Sun F,Li Q,Pang Y

    更新日期:2019-05-01 00:00:00

  • Immunologic significance of HLA class I genes in measles virus-specific IFN-gamma and IL-4 cytokine immune responses.

    abstract::The variability of immune responses modulated by human leukocyte antigen (HLA) genes and secreted cytokines is a significant factor in the development of a protective effect of measles vaccine. We studied the association between type 1 helper T cells (Th1)- and Th2-like cytokine immune responses and HLA class I allele...


    pub_type: 杂志文章


    authors: Ovsyannikova IG,Ryan JE,Vierkant RA,Pankratz VS,Jacobson RM,Poland GA

    更新日期:2005-12-01 00:00:00

  • Paucity of class I MHC gene heterogeneity between individuals in the endangered Hawaiian monk seal population.

    abstract::The Hawaiian monk seal population has experienced precipitous declines in the last 50 years. In this study, we provide evidence that individuals from remaining endangered population exhibit alarming uniformity in class I major histocompatibility (MHC) genes. The peripheral blood leukocyte-derived mRNA of six captive a...


    pub_type: 杂志文章


    authors: Aldridge BM,Bowen L,Smith BR,Antonelis GA,Gulland F,Stott JL

    更新日期:2006-04-01 00:00:00

  • An epitope on C4 beta light (L) chains detected by human anti-Rg; its relationship with beta chain polymorphism and MHC associations.

    abstract::Two out of ten Rg-specific antisera tested contain a third antibody specific for the beta chain of C4. Analysis of the beta chains of 66 unrelated individuals by sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that the epitope detected is located exclusively on the light (L) beta chain. A strong, bu...


    pub_type: 杂志文章


    authors: Robson T,Heard RN,Giles CM

    更新日期:1989-01-01 00:00:00

  • Genetic control of rat heart allograft rejection: effect of different MHC and non-MHC incompatibilities.

    abstract::We investigated the genetic control of heterotopic heart allograft rejection using a family of standard inbred, major histocompatibility complex (MHC)-congenic, and intra-MHC recombinant rat strains. Gene products of the various regions within the rat MHC differed markedly in their capacity to induce rejection. Isolat...


    pub_type: 杂志文章


    authors: Klempnauer J,Steiniger B,Lück R,Günther E

    更新日期:1989-01-01 00:00:00

  • H-2 influences phenytoin binding and inhibition of prostaglandin synthesis.

    abstract::We have reported that susceptibility to glucocorticoid- and phenytoin-induced cleft palate and glucocorticoid receptor levels in mice are influenced by the H-2 histocompatibility complex on chromosome 17. Phenytoin competes with glucocorticoids for the glucocorticoid receptor and inhibits production of prostaglandins ...


    pub_type: 杂志文章


    authors: Gupta C,Katsumata M,Goldman AS

    更新日期:1984-01-01 00:00:00

  • Polymorphic Bgl II restriction sites of DR alpha demarcate a novel HLA-DR1 antigen.

    abstract::Mechanisms to account for the unusual properties of a DR1 alpha beta complex (designated DRgp50) that is resistant to dissociation under normal conditions utilized were investigated. Expression of this DRgp50 complex is highly correlated with the failure of cells from certain DR1 individuals (DR1x) to stimulate specif...


    pub_type: 杂志文章


    authors: Davis JE,Cook RG,Van M,Rich RR

    更新日期:1988-01-01 00:00:00

  • A new ataxia-telangiectasia mutation in an 11-year-old female.

    abstract::Ataxia-telangiectasia (A-T), a rare inherited disorder, usually affects the nervous and immune systems, and occasionally other organs. A-T is associated mainly with mutations in the ataxia telangiectasia mutated (ATM) gene, which encodes a protein kinase that has a major role in the cellular response to DNA damage. We...


    pub_type: 杂志文章


    authors: Mortaz E,Marashian SM,Ghaffaripour H,Varahram M,Mehrian P,Dorudinia A,Garssen J,Adcock IM,Taylor M,Mahdaviani SA

    更新日期:2017-07-01 00:00:00

  • An informative set of SSLP markers and genomic profiles in the rat MHC, the RT1 complex.

    abstract::Almost 10,000 single nucleotide polymorphisms (SNPs) had been identified in the RT1 complex, the major histocompatibility complex of the rat, but less than approximately 0.5% have been characterized. In the context of the incomplete characterization of most SNPs, simple sequence length polymorphism (SSLP) marker devel...


    pub_type: 杂志文章


    authors: Takagi Y,Kuramoto T,Voigt B,Tsurumi T,Nakanishi S,Mashimo T,Masui N,Serikawa T

    更新日期:2009-03-01 00:00:00

  • Polymorphism of IL-10 gene promoter region: association with T cell proliferative responses after acellular pertussis vaccination in adults.

    abstract::Individual variation in immune responses is always encountered after vaccination. This phenomenon is also seen after acellular pertussis vaccination. The aim of this present study was to investigate whether single nucleotide polymorphisms (SNPs) in the IL-10 gene promoter region (rs1800890, rs1800896, rs1800871), IL-1...


    pub_type: 杂志文章


    authors: Gröndahl-Yli-Hannuksela K,Vahlberg T,Ilonen J,Mertsola J,He Q

    更新日期:2016-10-01 00:00:00

  • Molecular cloning of major histocompatibility complex class II B gene cDNA from the Bengalese finch Lonchura striata.

    abstract::The only avian major histocompatibility complex (Mhc) genes thus far identified are from species of the relatively small order of Galliformes, while by far the largest order of Passeriformes (songbirds), containing some 60% of extant bird species, has not been studied at all in this regard. The Galliformes emerged mor...


    pub_type: 杂志文章


    authors: Vincek V,Klein D,Graser RT,Figueroa F,O'hUigin C,Klein J

    更新日期:1995-01-01 00:00:00

  • The properties of the single chicken MHC classical class II alpha chain ( B-LA) gene indicate an ancient origin for the DR/E-like isotype of class II molecules.

    abstract::In mammals, there are MHC class II molecules with distinctive sequence features, such as the classical isotypes DR, DQ and DP. These particular isotypes have not been reported in non-mammalian vertebrates. We have isolated the class II (B-L) alpha chain from outbred chickens as the basis for the cloning and sequencing...


    pub_type: 杂志文章


    authors: Salomonsen J,Marston D,Avila D,Bumstead N,Johansson B,Juul-Madsen H,Olesen GD,Riegert P,Skjødt K,Vainio O,Wiles MV,Kaufman J

    更新日期:2003-12-01 00:00:00

  • H-2 antigen expression: Loss in vitro, restoration in vivo, and correlation with cell-mediated cytotoxicity in a mouse lymphoma cell line.

    abstract::The susceptibility to cell-mediated cytolysis of cells of the recently developed C57BL/Ka(H-2 ( b )) lymphoma cell line, BL/VL(3), was investigated in allogeneic assays with thymus-dependent lymphocytes (T cells). Compared to EL4, the widely used C57BL/6(H-2 ( b )) lymphoma cell line, BL/VL(3) cells were found to be i...


    pub_type: 杂志文章


    authors: Finn OJ,Lieberman M,Kaplan HS

    更新日期:1978-12-01 00:00:00

  • Altered structure of HLA class I heavy chains associated with mouse beta-2 microglobulin.

    abstract::The serological reactivities of HLA-A3, -B7, and -CW3 heavy chains associated with either mouse, bovine, or human beta-2-microglobulin (beta 2m) and expressed on the surface of transfected mouse fibroblasts were analyzed. All reactivities associated with one cluster (defined by monoclonal antibody W6/32) of antigenic ...


    pub_type: 杂志文章


    authors: Ferrier P,Fontecilla-Camps JC,Bucchini D,Caillol DH,Jordan BR,Lemonnier FA

    更新日期:1985-01-01 00:00:00

  • Novel and functional regulatory SNPs in the promoter region of FOXP3 gene in a Gabonese population.

    abstract::Parasites exert a selection pressure on their hosts and are accountable for driving diversity within gene families and immune gene polymorphisms in a host population. The overwhelming response of regulatory T cells during infectious challenges directs the host immune system to lose the ability to mount parasite specif...


    pub_type: 杂志文章


    authors: Hanel SA,Velavan TP,Kremsner PG,Kun JF

    更新日期:2011-07-01 00:00:00

  • Complementary DNA sequences encoding the rat MHC class II RT1-Bu and RT1-Du alpha and beta chains.

    abstract::The rat major histocompatibility complex loci RT1-B and RT1-D are equivalent to the human leucocyte antigens HLA-DQ and HLA-DR respectively. Here we describe the complementary DNA (cDNA) sequence encoding the alpha and beta chains of both the RT1-B and RT1-D locus genes of the rat RT1u haplotype. We have found entire ...


    pub_type: 杂志文章


    authors: Easterfield AJ,Bradley JA,Bolton EM

    更新日期:2003-08-01 00:00:00

  • Copy number variation and association over T-cell receptor genes--influence of DNA source.

    abstract::Genomic copy number variants (CNVs) are a common, heritable source of inter-individual differences in genomic sequence. Their influence on phenotypic variability and their involvement in the pathogenesis of several common diseases is well established and the object of many current studies. In the course of examining C...


    pub_type: 杂志文章


    authors: Schwienbacher C,De Grandi A,Fuchsberger C,Facheris MF,Svaldi M,Wjst M,Pramstaller PP,Hicks AA

    更新日期:2010-08-01 00:00:00

  • Structure and diversity of the heavy chain VDJ junctions in the developing Mexican axolotl.

    abstract::The immune capacity of young and adult axolotls (Ambystoma mexicanum) was evaluated by examining the combinatorial and junctional diversity of the VH chain. A large number of VDJ rearrangements isolated from 2.5-, 3.5-, 10-, and 24-month-old animals were sequenced. Six JH segments were identified with the canonical st...


    pub_type: 杂志文章


    authors: Golub R,Fellah JS,Charlemagne J

    更新日期:1997-01-01 00:00:00

  • Ancient features of the MHC class II presentation pathway, and a model for the possible origin of MHC molecules.

    abstract::Major histocompatibility complex (MHC) molecules are only found in jawed vertebrates and not in more primitive species. MHC class II type structures likely represent the ancestral structure of MHC molecules. Efficient MHC class II transport to endosomal compartments depends on association with a specialized chaperone,...


    pub_type: 杂志文章,评审


    authors: Dijkstra JM,Yamaguchi T

    更新日期:2019-03-01 00:00:00

  • Antigen-specific helper T cells recognize determinants encoded in the H-2D region of the H-2 gene complex.

    abstract::Observations have frequently been interpreted as showing that the helper T cells which collaborate with alloantigen-specific cytotoxic T-cell precursors can only recognize antigens encoded in the I region of the H-2 gene complex. An experimental system is described here that allows analysis of the recognition repertoi...


    pub_type: 杂志文章


    authors: Pilarski LM,Wegmann TG

    更新日期:1984-01-01 00:00:00

  • A new major histocompatibility complex class I b gene expressed in the mouse blastocyst and placenta.

    abstract::Because of the role major histocompatibility complex (MHC) class I b molecules may play during mouse embryonic development, we thought it would be interesting to search for additional MHC class I b molecules that might be expressed in preimplantation embryos, and in particular in the trophoblastic lineage. We therefor...


    pub_type: 杂志文章


    authors: Sipes SL,Medaglia MV,Stabley DL,DeBruyn CS,Alden MS,Catenacci V,Landel CP

    更新日期:1996-01-01 00:00:00