Isolated rat hepatocyte metabolism is affected by chronic renal failure.


:Metabolic changes due to chronic renal failure (CRF) were studied in isolated liver cells. In 14 CRF and 14 sham-operated rats, liver cells were isolated by the Berry and Friend method and incubated with various substrates in order to study gluconeogenesis, ureagenesis, ketogenesis, oxygen consumption as well as cytosolic and mitochondrial adenine nucleotide content. CRF rat hepatocytes exhibited a 25% to 45% decrease in gluconeogenesis and ureagenesis (P < 0.05) from all the tested substrates (lactate plus pyruvate, fructose, glycerol, dihydroxyacetone, alanine and glutamine for gluconeogenesis and alanine, glutamine, ammonia and ammonia plus ornithine for ureagenesis), while endogenous rates were unaffected. CRF did not alter ketone body production (acetoacetate and beta-hydroxybutyrate) from oleate or octanoate. In the presence of either oleate, lactate plus pyruvate or ammonia, oxygen uptake as well as cytosolic and mitochondrial total adenine nucleotides were unaffected by CRF, while the mitochondrial ATP/ADP ratio decreased (P < 0.001). Thus, this study of hepatocyte intermediary metabolism during CRF showed an alteration of only gluconeogenesis and ureagenesis pathways. Moreover, the association of normal oxygen uptake together with decreased mitochondrial ATP/ADP ratio suggest a possible increase in hepatocyte ATP demand during uremia.


Kidney Int


Kidney international


Cano N,Catelloni F,Fontaine E,Novaretti R,di Costanzo-Dufetel J,Reynier JP,Leverve XM




Has Abstract


1995-06-01 00:00:00














  • Klotho expression in osteocytes regulates bone metabolism and controls bone formation.

    abstract::Osteocytes within the mineralized bone matrix control bone remodeling by regulating osteoblast and osteoclast activity. Osteocytes express the aging suppressor Klotho, but the functional role of this protein in skeletal homeostasis is unknown. Here we identify Klotho expression in osteocytes as a potent regulator of b...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Komaba H,Kaludjerovic J,Hu DZ,Nagano K,Amano K,Ide N,Sato T,Densmore MJ,Hanai JI,Olauson H,Bellido T,Larsson TE,Baron R,Lanske B

    更新日期:2017-09-01 00:00:00

  • Effects of ACE inhibition and bradykinin antagonism on cardiovascular changes in uremic rats.

    abstract:BACKGROUND:Cardiovascular death continues to be a major problem in renal failure. Structural abnormalities of the heart and the vasculature contribute to the increased cardiovascular risk. They are ameliorated by angiotensin-converting enzyme (ACE) inhibitors, but because of the nonspecifity of ACE inhibition, it is un...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Amann K,Gassmann P,Buzello M,Orth SR,Törnig J,Gross ML,Magener A,Mall G,Ritz E

    更新日期:2000-07-01 00:00:00

  • The soluble urokinase receptor is not a clinical marker for focal segmental glomerulosclerosis.

    abstract::The soluble urokinase receptor (suPAR) promotes proteinuria and induces focal segmental glomerulosclerosis (FSGS)-like lesions in mice. A serum suPAR concentration cutoff of 3000 pg/ml has been proposed as a clinical biomarker for patients with FSGS. Interestingly, several studies in patients with glomerulopathy found...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Meijers B,Maas RJ,Sprangers B,Claes K,Poesen R,Bammens B,Naesens M,Deegens JK,Dietrich R,Storr M,Wetzels JF,Evenepoel P,Kuypers D

    更新日期:2014-03-01 00:00:00

  • Cyclosporine withdrawal and mycophenolate mofetil treatment effects on the progression of chronic cyclosporine nephrotoxicity.

    abstract:BACKGROUND:Recent clinical trials of mycophenolate mofetil (MMF) in chronic allograft nephropathy (CAN) demonstrated that the dose of cyclosporine A (CsA) is one of the critical factors in determining graft function in CAN, but the effect of MMF on chronic CsA nephropathy is undetermined. We undertook this study to eva...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Yang CW,Ahn HJ,Kim WY,Li C,Kim HW,Choi BS,Cha JH,Kim YS,Kim J,Bang BK

    更新日期:2002-07-01 00:00:00

  • Genotype-based changes in serum uric acid affect blood pressure.

    abstract::Elevated serum levels of uric acid consistently correlate with hypertension, but the directionality of the association remains debated. To help define this relationship, we used a controlled setting within a homogeneous Amish community and the Mendelian randomization of a nonsynonymous coding single-nucleotide polymor...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Parsa A,Brown E,Weir MR,Fink JC,Shuldiner AR,Mitchell BD,McArdle PF

    更新日期:2012-03-01 00:00:00

  • The financial effects of kidney stone prevention.

    abstract::Prevention of nephrolithiasis (NL) is now medically feasible and widely recommended. However, diagnosis and treatment of remediable causes of stones requires testing and drugs that impose a cost; this cost is balanced by the presumed reductions in stone related events and medical encounters. In order to assess the bal...

    journal_title:Kidney international

    pub_type: 临床试验,杂志文章


    authors: Parks JH,Coe FL

    更新日期:1996-11-01 00:00:00

  • Production of angiotensinogen and renin-like activity by rabbit proximal tubular cells in culture.

    abstract::Recent studies suggest the presence of local angiotensin generating system in the kidney. By using in situ hybridization technique, mRNA for angiotensinogen has been shown to be present in the proximal tubule. In the present study, we have attempted to examine the production of angiotensinogen and renin-like activity ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Yanagawa N,Capparelli AW,Jo OD,Friedal A,Barrett JD,Eggena P

    更新日期:1991-05-01 00:00:00

  • A GAP in our knowledge of vascular signaling in acute kidney injury.

    abstract::Injury resulting from ischemia-reperfusion injury is a multifactorial process involving compromised function in both the tubular and the vascular compartments. Multiple vasoactive compounds have been implicated in the profound vasoconstriction that occurs in response to ischemia-reperfusion injury, and many of these f...

    journal_title:Kidney international

    pub_type: 评论,杂志文章


    authors: Basile DP

    更新日期:2011-08-01 00:00:00

  • Collection, storage, preservation, and normalization of human urinary exosomes for biomarker discovery.

    abstract::Urinary exosomes containing apical membrane and intracellular fluid are normally secreted into the urine from all nephron segments, and may carry protein markers of renal dysfunction and structural injury. We studied methods for collection, storage, and preservation of urinary exosomal proteins. We collected urine fro...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Zhou H,Yuen PS,Pisitkun T,Gonzales PA,Yasuda H,Dear JW,Gross P,Knepper MA,Star RA

    更新日期:2006-04-01 00:00:00

  • High glucose can induce lipid peroxidation in the isolated rat glomeruli.

    abstract::The present study examined the effect of elevated glucose on glomerular cell membrane lipid peroxidation. Glomeruli were isolated from male Sprague-Dawley rat kidneys utilizing a standard sieving method and incubated in medium containing different concentrations of glucose at 37 degrees C for one and 48 hours. The lev...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Ha H,Yoon SJ,Kim KH

    更新日期:1994-12-01 00:00:00

  • Hyperhomocysteinemia in hemodialysis patients: effects of 12-month supplementation with hydrosoluble vitamins.

    abstract:BACKGROUND:High-efficiency hemodialysis may induce a deficiency in hydrosoluble vitamins. Supplementation with B-complex vitamins has been shown to lower serum homocysteine concentrations in several groups, but relatively few studies have concerned hemodialysis patients. Our objectives were to determine the status in B...

    journal_title:Kidney international

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Tremblay R,Bonnardeaux A,Geadah D,Busque L,Lebrun M,Ouimet D,Leblanc M

    更新日期:2000-08-01 00:00:00

  • Executive summary for the 2015 Annual Data Report of the China Kidney Disease Network (CK-NET).

    abstract::Chronic kidney disease (CKD) has received increased attention as a leading public health problem worldwide. Globally surveillance systems for kidney disease are still lacking, especially in developing countries like China, which constitutes an obstacle to develop effective preventive strategies. China Kidney Disease N...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Wang F,Yang C,Long J,Zhao X,Tang W,Zhang D,Bai K,Su Z,Gao B,Chu H,Wang J,Sun X,Wang S,Zuo L,Wang Y,Yu F,Wang H,Zhang L,Zhao MH

    更新日期:2019-03-01 00:00:00

  • Urinary CCN2 (CTGF) as a possible predictor of diabetic nephropathy: preliminary report.

    abstract:BACKGROUND:It is currently impossible to reliably predict which diabetic patients will develop nephropathy and progress to kidney failure. Microalbuminuria, often regarded as a predictor of overt diabetic renal disease is, in fact, an indicator of established glomerular damage. We have shown that glomerular expression ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Riser BL,Cortes P,DeNichilo M,Deshmukh PV,Chahal PS,Mohammed AK,Yee J,Kahkonen D

    更新日期:2003-08-01 00:00:00

  • In vivo unaltered muscle protein synthesis in experimental chronic metabolic acidosis.

    abstract::Chronic metabolic acidosis (CMA) is a major cause of growth defect, implying disturbances of protein metabolism. Previously, in vivo studies performed in the fasting state showed enhanced whole body protein turnover, whereas in vitro studies showed unchanged muscle protein synthesis. The present study is the first to ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Maniar S,Laouari D,Dechaux M,Motel V,Yvert JP,Mathian B,Kleinknecht C

    更新日期:1994-12-01 00:00:00

  • Nephrin mediates actin reorganization via phosphoinositide 3-kinase in podocytes.

    abstract::Nephrin is a slit diaphragm protein critical for structural and functional integrity of visceral glomerular epithelial cells (podocytes) and is known to be tyrosine phosphorylated by Src family kinases. We studied the role of phosphoinositide 3-kinase (PI3K), activated via the phosphorylation of nephrin, in actin cyto...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Zhu J,Sun N,Aoudjit L,Li H,Kawachi H,Lemay S,Takano T

    更新日期:2008-03-01 00:00:00

  • mTOR inhibitors may benefit kidney transplant recipients with mitochondrial diseases.

    abstract::Mitochondrial diseases represent a significant clinical challenge. Substantial efforts have been devoted to identifying therapeutic strategies for mitochondrial disorders, but effective interventions have remained elusive. Recently, we reported attenuation of disease in a mouse model of the human mitochondrial disease...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Johnson SC,Martinez F,Bitto A,Gonzalez B,Tazaerslan C,Cohen C,Delaval L,Timsit J,Knebelmann B,Terzi F,Mahal T,Zhu Y,Morgan PG,Sedensky MM,Kaeberlein M,Legendre C,Suh Y,Canaud G

    更新日期:2019-02-01 00:00:00

  • Effects of ibuprofen, naproxen, and sulindac on prostaglandins in men.

    abstract::In contrast to other nonsteroidal anti-inflammatory drugs (NSAIDs), sulindac has been reported to inhibit systemic prostaglandins (PGs) while not affecting renal PGs. We studied 11 normal volunteers who received placebo, ibuprofen, naproxen, or sulindac in a randomized, double-blind fashion. After control periods asse...

    journal_title:Kidney international

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Brater DC,Anderson S,Baird B,Campbell WB

    更新日期:1985-01-01 00:00:00

  • Nitric oxide synthase expression in AT2 receptor-deficient mice after DOCA-salt.

    abstract:BACKGROUND:Angiotensin II type 2 receptor-deficient mice (AT(2)-/y) provide an opportunity to study the relationship between the angiotensin II type 1 receptor (AT(1)) and nitric oxide synthase (NOS) isoforms without concomitant AT(2) receptor-related effects. To test this relationship, the expression of renal NOS isof...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Obst M,Gross V,Bonartsev A,Janke J,Müller DN,Park JK,Kärgel E,Luft FC

    更新日期:2004-06-01 00:00:00

  • Intragraft events preceding chronic renal allograft rejection in a modified tolerance protocol.

    abstract:BACKGROUND:Inbred miniature swine treated for 12 days with high-dose cyclosporine A develop tolerance to histocompatibility complex (MHC) class I-mismatched renal allografts. When this protocol was modified by adding thymectomy before transplant, all animals developed acute rejection. Thereafter, by day 100, one half d...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Shimizu A,Yamada K,Sachs DH,Colvin RB

    更新日期:2000-12-01 00:00:00

  • Blood pressure and long-term mortality in United States hemodialysis patients: USRDS Waves 3 and 4 Study.

    abstract:BACKGROUND:The long-term prognostic associations of pre- and post-dialysis blood pressures, interdialytic weight gain, and antihypertensive use in hemodialysis patients are unclear. METHODS:The United States Renal Data System (USRDS) Dialysis Morbidity and Mortality Waves 3 and 4 Study, a randomly generated sample of ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Foley RN,Herzog CA,Collins AJ,United States Renal Data System.

    更新日期:2002-11-01 00:00:00

  • Renoprotective effects of vitamin D analogs.

    abstract::Recent decades have witnessed the revelation of expanding roles of the vitamin D endocrine system beyond calcium and phosphorus metabolism. Along with these non-calcemic or non-classic actions of vitamin D are newly discovered therapeutic actions of vitamin D analogs in a number of pathological conditions, including k...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Li YC

    更新日期:2010-07-01 00:00:00

  • Osteopontin expression in angiotensin II-induced tubulointerstitial nephritis.

    abstract::Osteopontin is an arginine-glycine-aspartate (RGD) containing secreted phosphoprotein recently shown to stimulate a local macrophage influx when injected subcutaneously in mice. We examined the effect of angiotensin II infusion on renal injury and osteopontin expression in the rat kidney by in situ hybridization and i...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Giachelli CM,Pichler R,Lombardi D,Denhardt DT,Alpers CE,Schwartz SM,Johnson RJ

    更新日期:1994-02-01 00:00:00

  • Challenges in conducting clinical trials in nephrology: conclusions from a Kidney Disease-Improving Global Outcomes (KDIGO) Controversies Conference.

    abstract::Despite the high costs of treatment of people with kidney disease and associated comorbid conditions, the amount of reliable information available to guide the care of such patients is very limited. Some treatments have been assessed in randomized trials, but most such trials have been too small to detect treatment ef...

    journal_title:Kidney international



    authors: Baigent C,Herrington WG,Coresh J,Landray MJ,Levin A,Perkovic V,Pfeffer MA,Rossing P,Walsh M,Wanner C,Wheeler DC,Winkelmayer WC,McMurray JJV,KDIGO Controversies Conference on Challenges in the Conduct of Clinical Trials in Nephr

    更新日期:2017-08-01 00:00:00

  • Role of meprin A in renal tubular epithelial cell injury.

    abstract::Meprins are zinc-dependent metalloproteinases that are highly expressed in the brush-border membranes of both the kidney and the intestines. Meprins are capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. In this ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Herzog C,Seth R,Shah SV,Kaushal GP

    更新日期:2007-05-01 00:00:00

  • Continuous ambulatory peritoneal dialysis in the United States: a three-year study.

    abstract::This report of the NIH-supported National Continuous Ambulatory Peritoneal Dialysis (CAPD) Registry summarizes data on 7,404 patients treated with CAPD during the three-year period 1981 through 1983, or nearly one-half of patients treated in the USA who were treated with CAPD during this time. While age and diabetes m...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Nolph KD,Cutler SJ,Steinberg SM,Novak JW

    更新日期:1985-08-01 00:00:00

  • Regulatory interactions of alphabeta and gammadelta T cells in glomerulonephritis.

    abstract:BACKGROUND:Several lines of evidence suggest that cellular immune mechanisms contribute to glomerulonephritis. METHODS:The roles of alphabeta and gammadelta T cells in the pathogenesis of glomerulonephritis were investigated in a model of nephrotoxic nephritis in mice deficient in either T-cell population [T-cell rece...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Rosenkranz AR,Knight S,Sethi S,Alexander SI,Cotran RS,Mayadas TN

    更新日期:2000-09-01 00:00:00

  • Effect of spironolactone and captopril on nitric oxide and S-nitrosothiol formation in kidney of L-NAME-treated rats.

    abstract::Although angiotensin-converting enzyme (ACE) inhibitors are well-established drugs in the treatment of hypertension, they are not supposed to be sufficient in the inhibition of aldosterone formation. The present study analyzes the effect of aldosterone receptor antagonist, spironolactone and ACE inhibitor, captopril o...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Pechanova O,Matuskova J,Capikova D,Jendekova L,Paulis L,Simko F

    更新日期:2006-07-01 00:00:00

  • DNAzyme for TGF-beta suppressed extracellular matrix accumulation in experimental glomerulonephritis.

    abstract:BACKGROUND:We developed an electroporation-mediated gene transfer method targeting glomerular mesangial cells. Injecting DNA solution via renal artery followed by electric pulses using tweezers-type electrodes could result in efficient transfection in mesangial cells. Therefore, this gene transfer system opened a feasi...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Isaka Y,Nakamura H,Mizui M,Takabatake Y,Horio M,Kawachi H,Shimizu F,Imai E,Hori M

    更新日期:2004-08-01 00:00:00

  • Methicillin-resistant Staphylococcus aureus nasal carriage and infections in CAPD.

    abstract::In view of the increasing concern about methicillin-resistant Staphylococcus aureus (MRSA) infections, we studied the characteristics and outcome of MRSA nasal carriage and infections in our CAPD program. All patients entering into the CAPD program from January 1989 to December 1991 were enrolled into the study. The p...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Lye WC,Leong SO,Lee EJ

    更新日期:1993-06-01 00:00:00

  • Gelatinase A is a glomerular mesangial cell growth and differentiation factor.

    abstract::The members of the matrix metalloproteinase gene family play critical roles in numerous physiologic events, including cellular migration, tissue remodeling in wound healing and development, as well as in the evolution of the inflammatory process. The 72 kDa gelatinase A (formerly denoted 72 kDa Type IV collagenase) is...

    journal_title:Kidney international

    pub_type: 杂志文章,评审


    authors: Turck J,Pollock AS,Lovett DH

    更新日期:1997-05-01 00:00:00