当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Identification of adenovirus E1A regions which affect MHC class I expression and susceptibility to cytotoxic T lymphocytes.

Identification of adenovirus E1A regions which affect MHC class I expression and susceptibility to cytotoxic T lymphocytes.

Abstract：

:To map and characterize functional differences between the E1A oncoproteins of Ad5 and Ad12, we previously constructed a series of hybrid Ad5/12 E1A genes and used them in combination with Ad12 E1B to transform Hooded Lister rat cells. At least two regions within the first exon of Ad12 E1A which influenced tumorigenicity were identified. In this report, again using the hybrid Ad5/12 E1A (plus Ad12 E1B) transformants, we further examined the role of these regions in tumorigenicity by analyzing their effect on cell surface major histocompatibility complex class I expression and sensitivity to class I-restricted CD8+ cytotoxic T lymphocytes (CTLs). Results of these studies suggest that expression of either of the Ad12 E1A regions implicated in tumorigenicity could down-regulate cell surface class I levels. However, neither class I down-regulation nor sensitivity to allogeneic CTLs was shown to strictly correlate with the tumorigenic capacities of the transformed rat cells. Another factor influencing the tumorigenicity of Ad5 E1 and Ad12 E1 transformants may be the ability of their E1A products to encode CTL epitopes. To this end, we provide evidence suggesting that CTL epitopes may be encoded by Ad5 E1A but not by Ad12 E1A, since expression of certain portions of the Ad5 E1A protein conferred susceptibility to syngeneic Ad5 E1-specific CTLs in vitro, while Ad12 E1A expression did not confer susceptibility to syngeneic Ad12 E1-specific CTLs.

journal_name

Virology

journal_title

Virology

authors

Pereira DS,Rosenthal KL,Graham FL

doi

10.1006/viro.1995.1400

subject

Has Abstract

pub_date

1995-08-01 00:00:00

pages

268-77

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(85)71400-6

journal_volume

211

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Cloning of SV40 genomes from human brain tumors.

    abstract：:From two human brain tumors SV40 genomes were isolated by recombinant DNA techniques. The SV40 genome cloned from a human meningioma DNA was shown to be indistinguishable from wild-type SV40. In contrast, the SV40 genome cloned from a human astrocytoma proved to be a nonviable deletion mutant with a truncated early re...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(84)90357-x

    authors： Krieg P,Scherer G

    更新日期：1984-10-30 00:00:00

  • MMTV accessory factor Naf affects cellular gene expression.

    abstract：:Mouse mammary tumour virus (MMTV) encodes a viral superantigen (Sag) and a negative acting factor (Naf) which share parts of their coding sequence. Using 2-dimensional gel electrophoresis (2D-DIGE), we could show that at least 10 different cellular proteins were differentially expressed in Naf positive cells. Also, lu...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.10.029

    authors： Metzner C,Salmons B,Gunzburg WH,Gemeiner M,Miller I,Gesslbauer B,Kungl A,Dangerfield JA

    更新日期：2006-03-01 00:00:00

  • The outer capsid protein VP4 of murine rotavirus strain Eb represents a tentative new P type.

    abstract：:The nucleotide and deduced amino acid sequence of the gene 4 of murine rotavirus strain Eb were determined. The gene is 2359 nucleotides in length and encodes for a protein of 775 amino acids. Comparison of the VP4 amino acid sequence of the Eb strain with several human and animal rotavirus strains which represent all...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1153

    authors： Sereno MM,Gorziglia MI

    更新日期：1994-03-01 00:00:00

  • Analysis of rubella virus E1 glycosylation mutants expressed in COS cells.

    abstract：:cDNA clones encoding the envelope glycoprotein E1 of rubella virus (RV) were altered by site-directed mutagenesis at consensus sites for addition of N-linked glycans. The resulting plasmids were introduced into COS cells and the mutant E1 proteins were analyzed by indirect immunofluorescence, radioimmunoprecipitation,...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90915-x

    authors： Hobman TC,Qiu ZY,Chaye H,Gillam S

    更新日期：1991-04-01 00:00:00

  • Computational analysis of four human adenovirus type 4 genomes reveals molecular evolution through two interspecies recombination events.

    abstract：:Computational analysis of human adenovirus type 4 (HAdV-E4), a pathogen that is the only HAdV member of species E, provides insights into its zoonotic origin and molecular adaptation. Its genome encodes a domain of the major capsid protein, hexon, from HAdV-B16 recombined into the genome chassis of a simian adenovirus...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.05.014

    authors： Dehghan S,Seto J,Liu EB,Walsh MP,Dyer DW,Chodosh J,Seto D

    更新日期：2013-09-01 00:00:00

  • A variant of CRPV DNA preferentially maintained as a plasmid in NIH 3T3 cells and characterization of its transcripts in nude mouse tumors.

    abstract：:Rabbit and human papillomaviruses are strictly epitheliotropic and their DNA replicates extrachromosomally in benign lesions (warts) of their natural host. Their tissue and host specificity is thought to be genetically controlled and may account for the inefficiency in transforming heterologous cells. In our hands, CR...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90149-8

    authors： Nasseri M,Wettstein FO

    更新日期：1987-12-01 00:00:00

  • Regulation of a geminivirus coat protein promoter by AL2 protein (TrAP): evidence for activation and derepression mechanisms.

    abstract：:Tomato golden mosaic virus (TGMV) is a bipartite member of the subgroup III Geminiviridae. Like all geminiviruses, TGMV replicates in the nucleus of susceptible cells by rolling circle replication (RCR). Double-stranded replicative form DNA generated during RCR serves as template for the transcription of viral genes b...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8549

    authors： Sunter G,Bisaro DM

    更新日期：1997-06-09 00:00:00

  • A highly pathogenic porcine reproductive and respiratory syndrome virus generated from an infectious cDNA clone retains the in vivo virulence and transmissibility properties of the parental virus.

    abstract：:The nucleotide sequence of a highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) was determined. Transfection of MARC-145 cells with capped in vitro transcripts derived from a full-length cDNA clone of the viral genome resulted in infectious PRRSV with growth characteristics similar to that o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.04.046

    authors： Truong HM,Lu Z,Kutish GF,Galeota J,Osorio FA,Pattnaik AK

    更新日期：2004-08-01 00:00:00

  • Structure of the M2 protein gene of sonchus yellow net virus.

    abstract：:The nucleotide sequence of the gene immediately following the nucleocapsid protein gene of sonchus yellow net virus (SYNV), a plant rhabdovirus, is presented. Serological reactions of SYNV proteins with antibodies elicited by a fusion protein constructed from the sequenced gene indicate that this gene encodes an SYNV ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90190-5

    authors： Heaton LA,Zuidema D,Jackson AO

    更新日期：1987-11-01 00:00:00

  • Mutation and replacement of the 16-kDa protein gene in RNA-1 of tobacco rattle virus.

    abstract：:The function of the 16-kDa protein encoded by tobacco rattle virus (TRV) RNA-1 was investigated by a mutational analysis of the 16-kDa protein gene. Transcripts of TRV RNA-1 produced from a full-length cDNA clone of TRV RNA-1 (SYM strain) remained infectious when the 16-kDa protein gene was disrupted by premature term...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90601-7

    authors： Guilford PJ,Ziegler-Graff V,Baulcombe DC

    更新日期：1991-06-01 00:00:00

  • HIV pre-exposure prophylaxis: mucosal tissue drug distribution of RT inhibitor Tenofovir and entry inhibitor Maraviroc in a humanized mouse model.

    abstract：:Pre-exposure prophylaxis (PrEP) strategies utilizing anti-retroviral drugs show considerable promise for HIV prevention. However there is insufficient pharmacokinetic (PK) data on drug concentrations required for protection at the relevant mucosal tissues where the infection is initiated. Here we evaluated the utility...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.07.008

    authors： Veselinovic M,Yang KH,LeCureux J,Sykes C,Remling-Mulder L,Kashuba ADM,Akkina R

    更新日期：2014-09-01 00:00:00

  • Generation and characterization of a host cell-dependent gag gene mutant of human immunodeficiency virus type 1.

    abstract：:An in-frame gag gene mutant of human immunodeficiency virus type 1, which carries two amino acid substitutions in the center of the p24 coding region, was constructed in vitro, and its replication properties in several cell lines were examined. In CD4-negative SW480 cells transfected with the mutant clone, synthesis a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1478

    authors： Sakuragi J,Sakai H,Kawamura M,Tokunaga K,Ueda S,Adachi A

    更新日期：1995-09-10 00:00:00

  • Conserved aromatic residues of the hepatitis B virus Precore propeptide are involved in a switch between distinct dimeric conformations and essential in the formation of heterocapsids.

    abstract：:The Hepatitis B virus Precore protein, present in the secretory pathway as the HBeAg precursor, can associate in the cytoplasm with the Core protein to form heterocapsids, likely to favor viral persistence. Core and Precore proteins share their primary sequence except for ten additional aminoacids at the N-terminus of...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.06.013

    authors： Duriez M,Thouard A,Bressanelli S,Rossignol JM,Sitterlin D

    更新日期：2014-08-01 00:00:00

  • Plasticity of a critical antigenic determinant in the West Nile virus NY99 envelope protein domain III.

    abstract：:West Nile virus (WNV) is a mosquito-borne flavivirus that causes febrile illness, encephalitis, and occasionally death in humans. The envelope protein is the main component of the WNV virion surface, and domain III of the envelope protein (EIII) is both a putative receptor binding domain and a target of highly specifi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2016.05.024

    authors： Plante JA,Torres M,Huang CY,Beasley DWC

    更新日期：2016-09-01 00:00:00

  • Molecular cloning and mapping of rat cytomegalovirus DNA.

    abstract：:The DNA of a rat cytomegalovirus (RCMV) isolate from England is cleaved by HindIII into 25 fragments, 20 of which have been cloned into recombinant plasmids. Twenty-two of thirty KpnI fragments were similarly cloned which, together with the HindIII clones, resulted in cloning of 93% of the viral genome. Terminal fragm...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90155-9

    authors： Burns WH,Barbour GM,Sandford GR

    更新日期：1988-09-01 00:00:00

  • New highly divergent Plum pox virus isolates infecting sour cherry in Russia.

    abstract：:Unusual Plum pox virus (PPV) isolates (named Tat isolates) were discovered on sour cherry (Prunus cerasus) in Russia. They failed to be recognized by RT-PCR using commonly employed primers specific to the strains C or CR (the only ones that proved able to infect sour cherry) as well as to the strains M and W. Some of ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2016.12.016

    authors： Chirkov S,Ivanov P,Sheveleva A,Zakubanskiy A,Osipov G

    更新日期：2017-02-01 00:00:00

  • Transcriptional repression of K-Rta by Kaposi's sarcoma-associated herpesvirus K-bZIP is not required for oriLyt-dependent DNA replication.

    abstract：:Kaposi's sarcoma-associated herpesvirus origin-dependent DNA replication requires the core replication proteins plus K-Rta and K-bZIP. To determine which K-bZIP protein domains contribute to oriLyt-dependent DNA replication and facilitate suppression of K-Rta-mediated transcriptional activation, we generated a series ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.08.019

    authors： Rossetto C,Gao Y,Yamboliev I,Papousková I,Pari G

    更新日期：2007-12-20 00:00:00

  • Construction of an infectious DNA clone of the full-length human spumaretrovirus genome and mutagenesis of the bel 1 gene.

    abstract：:An infectious and full-length molecular clone of genomic human spumaretrovirus (HSRV) DNA was constructed. The infectivity of the pHSRV13 clone was demonstrated after transfection into susceptible cells by passage of HSRV-specific cytopathic effects as a cell-free culture supernatant, by electron microscopy of HSRV pa...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90820-2

    authors： Löchelt M,Zentgraf H,Flügel RM

    更新日期：1991-09-01 00:00:00

  • The nonstructural NSm protein of tomato spotted wilt virus induces tubular structures in plant and insect cells.

    abstract：:The expression and subcellular location of the 33.6-kDa nonstructural protein NSm of tomato spotted wilt virus (TSWV) was analyzed in Nicotiana rustica plants and protoplasts as a function of time. Immunofluorescent studies in protoplasts isolated from TSWV-infected N. rustica leaves showed that this protein could fir...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.0059

    authors： Storms MM,Kormelink R,Peters D,Van Lent JW,Goldbach RW

    更新日期：1995-12-20 00:00:00

  • The role of nucleocapsid of HIV-1 in virus assembly.

    abstract：:The role of the nucleocapsid protein of HIV-1 Gag in virus assembly was investigated using Gag truncation mutants, a nucleocapsid deletion mutant, and point mutations in the nucleocapsid region of Gag, in transfected COS cells, and in stable T-cell lines. Consistent with previous investigations, a truncation containin...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9374

    authors： Dawson L,Yu XF

    更新日期：1998-11-10 00:00:00

  • Two forms of the major barley stripe mosaic virus nonstructural protein are synthesized in vivo from alternative initiation codons.

    abstract：:Barley stripe mosaic virus (BSMV) has a tripartite genome comprising RNAs designated alpha, beta, and gamma, which collectively encode seven polypeptides. We show here that an antiserum raised against an abundant disease-specific protein from BSMV-infected plants reacts specifically with the viral beta b gene product ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90559-a

    authors： Petty IT,Jackson AO

    更新日期：1990-08-01 00:00:00

  • Virion stability and aphid vector transmissibility of Cucumber mosaic virus mutants.

    abstract：:The physical stability of virions of Cucumber mosaic virus (CMV) mutants was investigated to determine if relative stability correlated with efficiency of aphid transmission. Virion stability was evaluated by a urea disruption assay and by testing the infectivity of virus following purifications. All viruses were infe...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.11.021

    authors： Ng JC,Josefsson C,Clark AJ,Franz AW,Perry KL

    更新日期：2005-02-05 00:00:00

  • Characterization of human papillomavirus-11 mRNAs expressed in the context of autonomously replicating viral genomes.

    abstract：:We have previously adapted an experimental system which supports autonomous replication of human papillomavirus-11 (HPV-11) genomic DNA in a human squamous carcinoma cell line (SCC-4) following electroporation of linearized viral DNA. This system allows evaluation of HPV-11 transcription in the context of replicating ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0298

    authors： Renaud KJ,Cowsert LM

    更新日期：1996-06-01 00:00:00

  • Use of chimeric nectin-1(HveC)-related receptors to demonstrate that ability to bind alphaherpesvirus gD is not necessarily sufficient for viral entry.

    abstract：:Human nectin-1 (HveC, Prr1), a member of the immunoglobulin superfamily and a receptor for the entry of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1), binds to viral gD. For HSV-1, HSV-2, and PRV, the gD-binding region of nectin-1 has been localized to the N-...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.0989

    authors： Geraghty RJ,Fridberg A,Krummenacher C,Cohen GH,Eisenberg RJ,Spear PG

    更新日期：2001-07-05 00:00:00

  • Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: model systems for equine sarcoids.

    abstract：:It is now widely recognized that BPV-1 and less commonly BPV-2 are the causative agents of equine sarcoids. Here we present the generation of equine cell lines harboring BPV-1 genomes and expressing viral genes. These lines have been either explanted from sarcoid biopsies or generated in vitro by transfection of prima...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.11.037

    authors： Yuan ZQ,Gault EA,Gobeil P,Nixon C,Campo MS,Nasir L

    更新日期：2008-04-10 00:00:00

  • Synthesis and subcellular localization of the murine coronavirus nucleocapsid protein.

    abstract：:The synthesis and processing of the nucleocapsid protein (pp60) of the JHM strain of murine coronaviruses were examined. Pulse-chase experiments showed that pp60 was synthesized initially as a protein of approximately 57,000 in molecular weight (p57). Immunoprecipitation using mouse anti-JHMV antiserum indicated that ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90106-x

    authors： Stohlman SA,Fleming JO,Patton CD,Lai MM

    更新日期：1983-10-30 00:00:00

  • In vitro evidence that the coat protein of alfalfa mosaic virus plays a direct role in the regulation of plus and minus RNA synthesis: implications for the life cycle of alfalfa mosaic virus.

    abstract：:The coat protein of alfalfa mosaic virus has both structural and regulating functions. The latter is evident from the fact that the genomic RNAs of the virus, although they are of messenger polarity, cannot start an infection cycle in the absence of cost protein. The reason could be that the coat protein is needed for...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1189

    authors： De Graaff M,Man in't Veld MR,Jaspars EM

    更新日期：1995-04-20 00:00:00

  • Polymorphisms of the region of the Epstein-Barr virus genome which disrupts latency.

    abstract：:The nucleotide sequence of the portion of naturally occurring defective EBV (HR-1) DNA (designated het DNA) which is responsible for disruption of EBV latency has been determined and compared with the regions of the standard HR-1 viral genome from which it was derived. This rearranged 2.7-kbp DNA fragment represented ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90599-5

    authors： Jenson HB,Miller G

    更新日期：1988-08-01 00:00:00

  • A fused gene of nucleoprotein (NP) and herpes simplex virus genes (VP22) induces highly protective immunity against different subtypes of influenza virus.

    abstract：:We evaluated the immunogenicity and protective activity of plasmid DNA vaccines encoding the influenza virus NP gene (pNP) alone or in combination with the herpes simplex virus type 1 protein 22 gene (pVP22). Optimal immune responses were observed in BALB/c mice immunized with the combination of pVP22 plus pNP, as ass...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.04.015

    authors： Saha S,Yoshida S,Ohba K,Matsui K,Matsuda T,Takeshita F,Umeda K,Tamura Y,Okuda K,Klinman D,Xin KQ,Okuda K

    更新日期：2006-10-10 00:00:00

  • Hepatitis C virus core protein induces cell proliferation and activates ERK, JNK, and p38 MAP kinases together with the MAP kinase phosphatase MKP-1 in a HepG2 Tet-Off cell line.

    abstract：:Hepatitis C virus (HCV) core protein is a multifunctional protein interacting with cellular and viral proteins and promoters. A tetracycline-regulated system was used to generate a HepG2 Tet-Off cell line allowing regulated expression of a full-length (191 aa) and an N(c)-truncated core protein (160 aa). In this syste...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1227

    authors： Erhardt A,Hassan M,Heintges T,Häussinger D

    更新日期：2002-01-20 00:00:00