Abstract：
:We investigated the role of 3,5,3'-tri-iodothyronine (T3) in regulating differentiation of osteoprogenitor cells and also studied the effects of the glucocorticoid hormone dexamethasone (Dex) on the T3-induced effects on osteoprogenitor populations. This was done by determining the effects of either hormone alone, or of combinations of the two hormones, on the number of bone nodules formed in long-term cultures of rat calvaria cells. In this system, Dex has been shown to increase bone nodule formation, the maximal effective dose being 10 nM (Bellows et al. Endocrinology 121: 1985-1992; 1987). In standard culture medium containing 15% fetal bovine serum FBS), low concentrations of T3 (0.001-0.1 nM) had no effect on the number of bone nodules, while higher concentrations of 1-100 nM inhibited. However, in culture medium containing 10 nM Dex, the lower concentrations of T3 markedly increased the number of nodules. Short term pulse experiments with these low concentrations of T3 in the presence of Dex indicated that stimulation of nodule formation occurred only when T3 was present prior to confluency. Higher concentrations of T3 (1-100 nM) decreased nodule number whether or not Dex was added. We then cultured cells in medium containing FBS from which T3 and T4 were removed by treatment with AG-1 chi-10 resin. In both + or - Dex conditions, bone nodule formulation was increased 1.5 to 2-fold in T3, T4-depleted medium when compared with cultures maintained in standard culture medium.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Bonejournal_title
Boneauthors
Ishida H,Bellows CG,Aubin JE,Heersche JNdoi
10.1016/8756-3282(95)00102-jsubject
Has Abstractpub_date
1995-05-01 00:00:00pages
545-9issue
5eissn
8756-3282issn
1873-2763pii
875632829500102Jjournal_volume
16pub_type
杂志文章相关文献
BONE文献大全abstract：:Mechanical signal transduction in bone tissue begins with load-induced activation of several cellular pathways in the osteocyte population. A key pathway that participates in mechanotransduction is Wnt/Lrp5 signaling. A putative downstream mediator of activated Lrp5 is the nucleocytoplasmic shuttling protein β-catenin...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2016.04.028
更新日期：2016-07-01 00:00:00
abstract：:Matrix extracellular phosphoglycoprotein (MEPE) is an extracellular matrix protein that was first detected in tumor-induced osteomalacia (TIO). Investigations in mice revealed that MEPE is expressed in bone and teeth in a maturation-dependent manner, reaching its maximum during mineralization. However, from knockout e...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2004.03.033
更新日期：2004-08-01 00:00:00
abstract：:Juvenile osteoporosis (JO) is characterized by bone fragility during development, low bone mass and absence of extraskeletal features. Heterozygous loss-of-function mutations in LRP5 have been found in a few patients, but bone tissue and bone material abnormalities associated with such mutations have not been determin...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2013.07.020
更新日期：2013-11-01 00:00:00
abstract：:Vitamin K may be important in bone metabolism. Notably, high-dose menaquinone-4 (menatetrenone, MK4) has been reported to reduce ovariectomy (ovx)-induced bone loss in rats and to decrease osteoporotic fracture in postmenopausal women. However, it is unclear whether these beneficial effects reflect a physiologic effec...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/s8756-3282(02)00734-2
更新日期：2002-06-01 00:00:00
abstract：:Bone remodeling is a process involving both dynamic and static bone strain. Although there exist numerous studies on the effect of dynamic strain on implant stability and bone remodeling, the effect of static strain has yet to be clarified. Hence, for this purpose, the effect of static bone strain on implant stability...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2011.07.003
更新日期：2011-10-01 00:00:00
abstract：:Although basic fibroblast growth factor (bFGF) is a potent stimulator of bone formation when administered intravenously, less is known regarding the effects of this peptide on bone following subcutaneous (s.c.) administration. In addition, it is unknown whether coadministration of estrogen enhances the bone response t...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/s8756-3282(03)00118-2
更新日期：2003-09-01 00:00:00
abstract：:Feral adult female cynomolgus monkeys were divided into three groups and treated for two years: (1) normal controls; (2) weak androgenic treatment (androstenedione+estrone); and (3) strong androgenic treatment (testosterone). The tibiae and the trabecular bone of femoral head from each group were tested mechanically. ...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/8756-3282(95)00211-u
更新日期：1995-09-01 00:00:00
abstract：:The reversible calcitonin (CT)-induced inhibition of osteoclastic activity has been studied to clarify the mechanisms responsible for the so-called "escape phenomenon." Osteoclasts disaggregated from neonatal rabbits were cultured on glass coverslips or thin bovine bone slices. Resorption activity was evaluated by usi...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/8756-3282(89)90010-0
更新日期：1989-01-01 00:00:00
abstract：:Hypophosphatasia (HPP) is the inborn-error-of-metabolism characterized enzymatically by insufficient activity of the tissue-nonspecific isoenzyme of alkaline phosphatase (TNSALP) and caused by either mono- or bi-allelic loss-of-function mutation(s) of the gene ALPL that encodes this cell surface phosphomonoester phosp...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2019.02.025
更新日期：2019-05-01 00:00:00
abstract：PURPOSE:Traditional epidemiological studies suggest that there is an association between age at menarche (years) (AAM) and bone mineral density (BMD) at the sites of the femoral neck and lumbar spine (FNK and LS BMD), indicating a potentially important relationship between AAM and the development of osteoporosis (OP). ...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2018.09.015
更新日期：2018-12-01 00:00:00
abstract：:Previous work with cultured primary cells, from our group and from other laboratories, has shown that signals from extracellular matrix, transduced by integrins, play critical roles in regulating gene expression, tissue-specific differentiation, and survival of primary osteoblasts and fibroblasts. This summary will fo...
journal_title：Bone
pub_type： 杂志文章,评审
doi：10.1016/s8756-3282(99)00106-4
更新日期：1999-07-01 00:00:00
abstract：BACKGROUND:Prior research on the ability of bisphosphonates to prevent fractures and related health care utilization has been based on high levels of compliance achievable in clinical trial settings. This study was undertaken to assess rates of osteoporotic fractures and health care utilization as a function of bisphos...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2007.07.009
更新日期：2007-11-01 00:00:00
abstract：:The aim of this study was to provide insight into the bone mineral status and biochemical markers of bone metabolism in a Chinese population from Shenyang, in the north of China, where hip fracture incidence is low. A total of 194 healthy men and women, aged 25-35 years and 65-75 years, were studied. Bone mineral dens...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/s8756-3282(01)00676-7
更新日期：2002-02-01 00:00:00
abstract：:In 1994, the World Health Organization published diagnostic criteria for osteoporosis. Since then, many new technologies have been developed for the measurement of bone mineral at multiple skeletal sites. The information provided by each assessment will describe the clinical characteristics, fracture risk and epidemio...
journal_title：Bone
pub_type： 杂志文章,评审
doi：10.1016/j.bone.2007.11.001
更新日期：2008-03-01 00:00:00
abstract：:There is a lot of interest for how and how much osteoclasts resorb bone. However, little is known about the mechanism which controls the orientation and the duration of a resorptive event, thereby determining the specific geometry of a cavitation. Here we show that the relative rate of collagenolysis vs. demineralizat...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2013.06.007
更新日期：2013-09-01 00:00:00
abstract：:As the world's population ages, the occurrence of osteoporosis-related fractures is projected to increase. Low areal bone mineral density (aBMD), a well-known risk factor for fractures, may be influenced by physical activity (PA). In this cross-sectional study, we aimed to investigate potential associations between ob...
journal_title：Bone
pub_type： 临床试验,杂志文章
doi：10.1016/j.bone.2015.07.001
更新日期：2015-12-01 00:00:00
abstract：:Bisphosphonates increase bone mass and reduce fracture risk, but their anti-resorptive action may lead to increases in fatigue microdamage. To investigate how bisphosphonate effects influence changes in bone volume and microdamage in the long term, a strain-adaptive model of bone remodeling and microdamage balance was...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2004.03.007
更新日期：2004-07-01 00:00:00
abstract：:Bone marrow mesenchymal stem cells (BMMSCs) provide the biological basis for bone reconstruction. Mechanical tension stimulation as a potent modulator is able to promote osteogenic capability of BMMSCs. Long non-coding RNAs (LncRNAs) as competing endogenous RNAs (ceRNAs) for microRNAs, are postulated to regulate the o...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2017.12.013
更新日期：2018-03-01 00:00:00
abstract：:Recent increasing evidence supports a role for neuronal type signaling in bone. Specifically glutamate receptors have been found in cells responsible for bone remodeling, namely the osteoblasts and the osteoclasts. While most studies have focused on ionotropic glutamate receptors, the relevance of the metabotropic glu...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2016.10.025
更新日期：2017-01-01 00:00:00
abstract：PURPOSE:Low trauma rib fracture (hereinafter, rib fracture) is common in the elderly, but its risk factors and mortality consequence are rarely studied. We sought to define the epidemiology of rib fracture and the association between rib fracture and postfracture mortality. METHODS:The study was part of the Dubbo Oste...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2018.08.016
更新日期：2018-11-01 00:00:00
abstract：:The rat model has previously been used to test the effect of ovariectomy and of PTH administration on vertebral bone mass, size, and biomechanical competence. In this study, we used the same "biomechanical rat model" to assess the effect of long-term exercise on vertebral bone mass and quality and also on femoral bone...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/8756-3282(94)90291-7
更新日期：1994-05-01 00:00:00
abstract：:In order to investigate the ultrastructure of bone resorptive cells in the two types of adult benign human osteopetrosis, iliac crest biopsies were obtained from 11 patients and 10 normal males, who served as a control group. Six patients had the radiological type I (4 women, 2 men, aged 23-58 years, mean = 36.5 years...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/8756-3282(93)90316-3
更新日期：1993-11-01 00:00:00
abstract：:Metastatic bone disease has a major impact on the morbidity and mortality of breast cancer patients, and studies on bone metastasis biology have led to the development of the most widely used drugs for bone metastases treatment: zoledronate (Zol) and denosumab (Den). The aim of the present study was to assess the effe...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2014.06.017
更新日期：2014-09-01 00:00:00
abstract：:Low-intensity pulsed ultrasound (LIPUS) has distinct effects on biologic mineralization at intensities of <100 mW/cm2. Intensity-dependent differences in the pattern of accelerated mineralization may be due to different alterations in regulation of collagenous matrix formation. However, little is known about the influ...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2004.04.024
更新日期：2004-09-01 00:00:00
abstract：:Fractal analysis is a method for describing complex shapes, including the cancellous structure of bone. It describes the surface texture and form of individual trabecular profiles and the overall cancellous structure. Sixty-four postmenopausal women with symptoms of back pain were referred for investigation for osteop...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/s8756-3282(98)00063-5
更新日期：1998-07-01 00:00:00
abstract：:We performed a comprehensive analysis of the relationships between histologic indices in the iliac crest (cancellous bone volume, trabecular structural indices, cortical width, and core width) and bone density in the spine, hip, and wrist in 81 patients with various metabolic bone diseases including osteoporosis, oste...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/8756-3282(92)90203-9
更新日期：1992-01-01 00:00:00
abstract：:PEBP2alphaA/AML3/CBFA1 is one of the transcription regulators that belong to the PEBP2/AML family. The knockout mice, where the gene encoding PEBP2alphaA/AML3/CBFA1 was inactivated, showed no osteogenesis, indicating the critical role of this transcription factor in osteoblastic differentiation (Komori, Y. et al. Cell...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/s8756-3282(97)00267-6
更新日期：1998-02-01 00:00:00
abstract：:Exercise during growth can be important for attaining optimal bone mass. High-intensity long-duration protocols, however, can have detrimental effects on immature bone morphology and mechanics. The underlying mechanisms are poorly understood. Here, we quantified the mechanical environment of the middiaphyseal rooster ...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/s8756-3282(99)00256-2
更新日期：2000-02-01 00:00:00
abstract：:The study reports a theranostic nature of rno-miR-300 (miR300) in the osteoblast functioning, by influencing the signaling pathway(s), associated with osteoblast differentiation. Excessive expression of miR300 suppresses osteoblast functions. Smad3 served as a validated target for miR300, on homology-based computation...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2020.115603
更新日期：2021-02-01 00:00:00
abstract：:Rough-surfaced substrates made by a variety of methods have been shown to influence osteoblast proliferation and differentiation. The purpose of this study is to confirm the role of surface roughness in promoting osteoblastic differentiation using tissue culture polystyrene as substrate, by excluding factors other tha...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/s8756-3282(99)00192-1
更新日期：1999-10-01 00:00:00
