Tri-iodothyronine (T3) and dexamethasone interact to modulate osteoprogenitor cell differentiation in fetal rat calvaria cell cultures.


:We investigated the role of 3,5,3'-tri-iodothyronine (T3) in regulating differentiation of osteoprogenitor cells and also studied the effects of the glucocorticoid hormone dexamethasone (Dex) on the T3-induced effects on osteoprogenitor populations. This was done by determining the effects of either hormone alone, or of combinations of the two hormones, on the number of bone nodules formed in long-term cultures of rat calvaria cells. In this system, Dex has been shown to increase bone nodule formation, the maximal effective dose being 10 nM (Bellows et al. Endocrinology 121: 1985-1992; 1987). In standard culture medium containing 15% fetal bovine serum FBS), low concentrations of T3 (0.001-0.1 nM) had no effect on the number of bone nodules, while higher concentrations of 1-100 nM inhibited. However, in culture medium containing 10 nM Dex, the lower concentrations of T3 markedly increased the number of nodules. Short term pulse experiments with these low concentrations of T3 in the presence of Dex indicated that stimulation of nodule formation occurred only when T3 was present prior to confluency. Higher concentrations of T3 (1-100 nM) decreased nodule number whether or not Dex was added. We then cultured cells in medium containing FBS from which T3 and T4 were removed by treatment with AG-1 chi-10 resin. In both + or - Dex conditions, bone nodule formulation was increased 1.5 to 2-fold in T3, T4-depleted medium when compared with cultures maintained in standard culture medium.(ABSTRACT TRUNCATED AT 250 WORDS)






Ishida H,Bellows CG,Aubin JE,Heersche JN




Has Abstract


1995-05-01 00:00:00
















  • Gastrodin: an ancient Chinese herbal medicine as a source for anti-osteoporosis agents via reducing reactive oxygen species.

    abstract::Increased levels of reactive oxygen species (ROS) are a crucial pathogenic factor of osteoporosis. Gastrodin, isolated from the traditional Chinese herbal agent Gastrodia elata, is a potent antioxidant. We hypothesized that gastrodin demonstrates protective effects against osteoporosis by partially reducing reactive o...


    pub_type: 杂志文章


    authors: Huang Q,Shi J,Gao B,Zhang HY,Fan J,Li XJ,Fan JZ,Han YH,Zhang JK,Yang L,Luo ZJ,Liu J

    更新日期:2015-04-01 00:00:00

  • Prolonged alendronate treatment prevents the decline in serum TGF-β1 levels and reduces cortical bone strength in long-term estrogen deficiency rat model.

    abstract:INTRODUCTION:While the anti-resorptive effects of the bisphosphonates (BPs) are well documented, many questions remain about their mechanisms of action, particularly following long-term use. This study evaluated the effects of alendronate (Ale) treatment on TGF-β1 signaling in mesenchymal stem cells (MSCs) and osteocyt...


    pub_type: 杂志文章


    authors: Jia J,Yao W,Amugongo S,Shahnazari M,Dai W,Lay YA,Olvera D,Zimmermann EA,Ritchie RO,Li CS,Alliston T,Lane NE

    更新日期:2013-01-01 00:00:00

  • Hip fractures: incidence, risk factors, energy absorption, and prevention.

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    pub_type: 杂志文章,评审


    authors: Lauritzen JB

    更新日期:1996-01-01 00:00:00

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    abstract::The increasing application of approaches that allow tracing of individual cells over time, together with transcriptomic and epigenomic analyses is changing the way resident stromal stem cells (mesenchymal stem cells) are viewed. Rather than being a defined, homogeneous cell population as described following in vitro e...


    pub_type: 杂志文章


    authors: Yianni V,Sharpe PT

    更新日期:2020-05-01 00:00:00

  • Thermostable (SULT1A1) and thermolabile (SULT1A3) phenol sulfotransferases in human osteosarcoma and osteoblast cells.

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    authors: Dubin RL,Hall CM,Pileri CL,Kudlacek PE,Li XY,Yee JA,Johnson ML,Anderson RJ

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  • Changes in Dkk-1, sclerostin, and RANKL serum levels following discontinuation of long-term denosumab treatment in postmenopausal women.

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    authors: Fassio A,Adami G,Benini C,Vantaggiato E,Saag KG,Giollo A,Lippolis I,Viapiana O,Idolazzi L,Orsolini G,Rossini M,Gatti D

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  • Increased periosteal circumference remains present 12 months after an exercise intervention in preschool children.

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    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Specker B,Binkley T

    更新日期:2004-12-01 00:00:00

  • Changes in tibial bone microarchitecture in female recruits in response to 8 weeks of U.S. Army Basic Combat Training.

    abstract:BACKGROUND:U.S. Army Basic Combat Training (BCT) is a physically-demanding program at the start of military service. Whereas animal studies have shown that increased mechanical loading rapidly alters bone structure, there is limited evidence of changes in bone density and structure in humans exposed to a brief period o...


    pub_type: 杂志文章


    authors: Hughes JM,Gaffney-Stomberg E,Guerriere KI,Taylor KM,Popp KL,Xu C,Unnikrishnan G,Staab JS,Matheny RW Jr,McClung JP,Reifman J,Bouxsein ML

    更新日期:2018-08-01 00:00:00

  • Severe bone loss and multiple fractures in SCN8A-related epileptic encephalopathy.

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    pub_type: 杂志文章


    authors: Rolvien T,Butscheidt S,Jeschke A,Neu A,Denecke J,Kubisch C,Meisler MH,Pueschel K,Barvencik F,Yorgan T,Oheim R,Schinke T,Amling M

    更新日期:2017-10-01 00:00:00

  • Whole-exome sequencing reveals a heterozygous LRP5 mutation in a 6-year-old boy with vertebral compression fractures and low trabecular bone density.

    abstract::Juvenile osteoporosis (JO) is characterized by bone fragility during development, low bone mass and absence of extraskeletal features. Heterozygous loss-of-function mutations in LRP5 have been found in a few patients, but bone tissue and bone material abnormalities associated with such mutations have not been determin...


    pub_type: 杂志文章


    authors: Fahiminiya S,Majewski J,Roughley P,Roschger P,Klaushofer K,Rauch F

    更新日期:2013-11-01 00:00:00

  • Association of klotho gene polymorphism with bone density and spondylosis of the lumbar spine in postmenopausal women.

    abstract::Based on the fact that the klotho-deficient mouse exhibits multiple aging phenotypes, including osteopenia and subchondral sclerosis of joints, we explored the possibility of whether human klotho gene polymorphism is associated with two major age-related skeletal disorders: osteoporosis and spondylosis. Analysis of th...


    pub_type: 杂志文章


    authors: Ogata N,Matsumura Y,Shiraki M,Kawano K,Koshizuka Y,Hosoi T,Nakamura K,Kuro-O M,Kawaguchi H

    更新日期:2002-07-01 00:00:00

  • Three-dimensional cultures of normal human osteoblasts: proliferation and differentiation potential in vitro and upon ectopic implantation in nude mice.

    abstract::We report the establishment in vitro of three-dimensional (3D) cultures of human osteoblasts (hOB) derived from normal adults and supported uniquely by the extracellular matrix (ECM) they deposit. Osteoblasts were cultured in 3D cultures in vitro for up to 120 days. The 3D cultures, examined at 25, 31, and 48 days, ex...


    pub_type: 杂志文章


    authors: Ferrera D,Poggi S,Biassoni C,Dickson GR,Astigiano S,Barbieri O,Favre A,Franzi AT,Strangio A,Federici A,Manduca P

    更新日期:2002-05-01 00:00:00

  • Postnatal β-catenin deletion from Dmp1-expressing osteocytes/osteoblasts reduces structural adaptation to loading, but not periosteal load-induced bone formation.

    abstract::Mechanical signal transduction in bone tissue begins with load-induced activation of several cellular pathways in the osteocyte population. A key pathway that participates in mechanotransduction is Wnt/Lrp5 signaling. A putative downstream mediator of activated Lrp5 is the nucleocytoplasmic shuttling protein β-catenin...


    pub_type: 杂志文章


    authors: Kang KS,Hong JM,Robling AG

    更新日期:2016-07-01 00:00:00

  • Bisphosphonates--history and experimental basis.

    abstract::This paper reviews the mechanisms of action of the bisphosphonates, a new class of recently developed drugs. The geminal bisphosphonates are characterized by a P-C-P bond and are thus analogs of pyrophosphate. They bind strongly to hydroxyapatite crystals and in vitro inhibit both crystal formation and dissolution. In...


    pub_type: 历史文章,杂志文章,评审


    authors: Fleisch H

    更新日期:1987-01-01 00:00:00

  • Loss of myostatin (GDF8) function increases osteogenic differentiation of bone marrow-derived mesenchymal stem cells but the osteogenic effect is ablated with unloading.

    abstract::Myostatin (GDF8) is a negative regulator of skeletal muscle growth and mice lacking myostatin show a significant increase in muscle mass and bone density compared to normal mice. In order to further define the role of myostatin in regulating bone mass we sought to determine if loss of myostatin function significantly ...


    pub_type: 杂志文章


    authors: Hamrick MW,Shi X,Zhang W,Pennington C,Thakore H,Haque M,Kang B,Isales CM,Fulzele S,Wenger KH

    更新日期:2007-06-01 00:00:00

  • Objectively measured physical activity is associated with parameters of bone in 70-year-old men and women.

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    pub_type: 临床试验,杂志文章


    authors: Johansson J,Nordström A,Nordström P

    更新日期:2015-12-01 00:00:00

  • Sclerostin levels and bone turnover markers in adolescents with anorexia nervosa and healthy adolescent girls.

    abstract::Sclerostin, product of the SOST gene, is an important determinant of bone formation and resorption. Adolescents with anorexia nervosa (AN) have low bone density and decreased levels of bone turnover markers. However, sclerostin has not been examined in AN as a potential mediator of impaired bone metabolism. Our study ...


    pub_type: 杂志文章,随机对照试验


    authors: Faje AT,Fazeli PK,Katzman DK,Miller KK,Breggia A,Rosen CJ,Mendes N,Klibanski A,Misra M

    更新日期:2012-09-01 00:00:00

  • Uncoupling of bone metabolism in rheumatoid arthritis patients with or without joint destruction: assessment with serum type I collagen breakdown products.

    abstract::In this study we investigate bone metabolism in patients with rheumatoid arthritis (RA), with or without joint destruction, using serum biochemical markers of bone turnover. Three hundred eighteen patients (disease duration >2 years; mean 9 years) were divided into those with joint destruction, that is, with Larsen wr...


    pub_type: 杂志文章


    authors: Garnero P,Jouvenne P,Buchs N,Delmas PD,Miossec P

    更新日期:1999-04-01 00:00:00

  • Association between fluoride, magnesium, aluminum and bone quality in renal osteodystrophy.

    abstract:INTRODUCTION:Trace elements are known to influence bone metabolism; however, their effects may be exacerbated in renal failure because dialysis patients are unable to excrete excess elements properly. Our study correlated bone quality in dialysis patients with levels of bone fluoride, magnesium, and aluminum. A number ...


    pub_type: 杂志文章


    authors: Ng AH,Hercz G,Kandel R,Grynpas MD

    更新日期:2004-01-01 00:00:00

  • Overexpression of FGFR3, Stat1, Stat5 and p21Cip1 correlates with phenotypic severity and defective chondrocyte differentiation in FGFR3-related chondrodysplasias.

    abstract::Achondroplasia (ACH) and thanatophoric dysplasia (TD) are human skeletal disorders of increasing severity accounted for by mutations in the fibroblast growth factor receptor 3 (FGFR3). Attempts to elucidate the molecular signaling pathways leading to these phenotypes through mouse model engineering have provided relev...


    pub_type: 杂志文章


    authors: Legeai-Mallet L,Benoist-Lasselin C,Munnich A,Bonaventure J

    更新日期:2004-01-01 00:00:00

  • The bisphosphonate zoledronic acid decreases tumor growth in bone in mice with defective osteoclasts.

    abstract::Bisphosphonates (BPs), bone targeted drugs that disrupt osteoclast function, are routinely used to treat complications of bone metastasis. Studies in preclinical models of cancer have shown that BPs reduce skeletal tumor burden and increase survival. Similarly, we observed in the present study that administration of t...


    pub_type: 杂志文章


    authors: Hirbe AC,Roelofs AJ,Floyd DH,Deng H,Becker SN,Lanigan LG,Apicelli AJ,Xu Z,Prior JL,Eagleton MC,Piwnica-Worms D,Rogers MJ,Weilbaecher K

    更新日期:2009-05-01 00:00:00

  • Fourier transform infrared microspectroscopic analysis of bones of osteocalcin-deficient mice provides insight into the function of osteocalcin.

    abstract::Osteocalcin, the gamma-carboxyglutamic acid-containing protein, which in most species is the predominant noncollagenous protein of bone and dentin, has been postulated to play roles in bone formation and remodeling. Recently, genetic studies showed that osteocalcin acts as an inhibitor of osteoblast function. Based on...


    pub_type: 杂志文章


    authors: Boskey AL,Gadaleta S,Gundberg C,Doty SB,Ducy P,Karsenty G

    更新日期:1998-09-01 00:00:00

  • Has reimbursement of bone mineral density testing and anti-osteoporotic treatments improved management of osteoporosis in France?

    abstract:BACKGROUND:Since July 1, 2006 bone mineral density (BMD) testing has been reimbursed for osteoporosis screening under specific clinical conditions in France. Concurrently, drug coverage has been extended to all anti-osteoporotic treatments in women with low BMD. It was thus expected that the utilisation of BMD testing ...


    pub_type: 杂志文章


    authors: Canoui-Poitrine F,Jaglal S,Chapurlat R,Tainturier V,Colin C,Schott AM

    更新日期:2010-10-01 00:00:00

  • Novel SLC34A3 mutation causing hereditary hypophosphataemic rickets with hypercalciuria in a Gambian family.

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    pub_type: 杂志文章


    authors: Braithwaite V,Pettifor JM,Prentice A

    更新日期:2013-03-01 00:00:00

  • Comparative serum proteome expression of osteonecrosis of the femoral head in adults.

    abstract::Osteonecrosis of the femoral head (ONFH) is a skeletal disorder characterized by ischemic deterioration, bone marrow edema and eventually femoral head collapse. The systemic regulation of ONFH in adult patients has not been examined. Serum proteomic is an innovative tool that potentially detects simultaneous expressio...


    pub_type: 杂志文章


    authors: Wu RW,Wang FS,Ko JY,Wang CJ,Wu SL

    更新日期:2008-09-01 00:00:00

  • Effect of early nutritional intake on long-term growth and bone mineralization of former very low birth weight infants.

    abstract:BACKGROUND:Preterm infants are at risk for impaired bone mineralization and growth in length later in life due to inadequate nutritional intake in the early postnatal period. OBJECTIVE:To investigate whether increased nutritional supplementation of calcium, phosphate and protein in Very Low Birth Weight (VLBW) infants...


    pub_type: 杂志文章


    authors: Christmann V,van der Putten ME,Rodwell L,Steiner K,Gotthardt M,van Goudoever JB,van Heijst AFJ

    更新日期:2018-03-01 00:00:00

  • A nonhuman primate model of age-related bone loss: a longitudinal study in male and premenopausal female rhesus monkeys.

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    pub_type: 杂志文章


    authors: Black A,Tilmont EM,Handy AM,Scott WW,Shapses SA,Ingram DK,Roth GS,Lane MA

    更新日期:2001-03-01 00:00:00

  • Increased nitric oxide-mediated vasodilation of bone resistance arteries is associated with increased trabecular bone volume after endurance training in rats.

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    pub_type: 杂志文章


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  • Biot theory: a review of its application to ultrasound propagation through cancellous bone.

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    pub_type: 杂志文章,评审


    authors: Haire TJ,Langton CM

    更新日期:1999-04-01 00:00:00

  • Differences in the microarchitectural features of the lateral collapsed lesion between osteonecrosis and subchondral insufficiency fracture of the femoral head.

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    pub_type: 杂志文章


    authors: Kawano K,Motomura G,Ikemura S,Yamaguchi R,Baba S,Xu M,Nakashima Y

    更新日期:2020-12-01 00:00:00