Isolation of endo A cDNA from mouse 8-cell stage embryos.

Abstract:

:To analyse the species of genes expressed in a cleavage stage mouse embryo, we have constructed a cDNA library containing 3.0 x 10(5) independent clones from about 2 x 10(3) embryos at the 8-cell stage of development. Endo A cDNA prepared from parietal yolk sac endoderm like PYS-2 cells was used to screen the library. Southern blot analyses using the endo A sequence as a probe and restriction mapping analyses revealed that four independent recombinants had been inserted endo A sequence. Sequencing data of these clones showed that endo A mRNA present in the 8-cell stage embryo is identical to that of parietal yolk sac endoderm cells.

authors

Nozaki M,Murata K,Morita T,Matsushiro A

doi

10.1016/0006-291x(88)90223-9

subject

Has Abstract

pub_date

1988-08-15 00:00:00

pages

890-4

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(88)90223-9

journal_volume

154

pub_type

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