Substantial early loss of induced pluripotent stem cells following transplantation in myocardial infarction.


:The limited success of cardiac stem cell therapy has lately generated discussion regarding its effectiveness. We hypothesized that immediate cell loss after intramyocardial injection significantly obscures the regenerative potential of stem cell therapy. Therefore, our aim was to assess the distribution and quantity of induced pluripotent stem cells after intramyocardial delivery using in vivo bioluminescence analysis. In this context, we wanted to investigate if the injection of different cell concentrations would exert influence on cardiac cell retention. Murine-induced pluripotent stem cells were transfected for luciferase reporter gene expression and transplanted into infarcted myocardium in mice after left anterior descending coronary artery ligation. Cells were delivered constantly in aqueous media (15 μL) in different cell concentrations (group A, n = 10, 5.0 × 10(5) cells; group B, n = 10, 1.0 × 10(6) cells). Grafts were detected using bioluminescence imaging. Organ explants were imaged 10 min after injection to quantify early cardiac retention and cell biodistribution. Bioluminescence imaging showed a massive early displacement from the injection site to the pulmonary circulation, leading to lung accumulation. Mean cell counts of explanted organs in group A were 7.51 × 10(4) ± 4.09 × 10(3) (heart), 6.44 × 10(4) ± 2.48 × 10(3) (left lung), and 8.06 × 10(5) ± 3.61 × 10(3) (right lung). Respective cell counts in group B explants were 1.69 × 10(5) ± 7.69 × 10(4) (heart), 2.11 × 10(5) ± 4.58 × 10(3) (left lung), and 3.25 × 10(5) ± 9.35 × 10(3) (right lung). Applying bioluminescence imaging, we could unveil and quantify massive early cardiac stem cell loss and pulmonary cell accumulation following intramyocardial injection. Increased injection concentrations led to much higher intracardiac cell counts; however, pulmonary biodistribution of transplanted cells still persisted. Therefore, we recommend applying tissue engineering techniques for cardiac stem cell transplantations in order to improve cardiac retention and limit biodistribution.


Artif Organs


Artificial organs


Martens A,Rojas SV,Baraki H,Rathert C,Schecker N,Zweigerdt R,Schwanke K,Rojas-Hernandez S,Martin U,Saito S,Schmitto JD,Haverich A,Kutschka I




Has Abstract


2014-11-01 00:00:00












  • Experimental high-volume hemofiltration with predilutional tris-hydroxymethylaminomethane for correction of low tidal volume ventilation-induced acidosis.

    abstract::The most common method of controlling acidemia during lung-protective ventilation is CO₂ removal with an extracorporeal lung assist (ECLA) system. Another possibility to prevent acidemia is based on intravenous (i.v.) application of tris-hydroxymethyl-aminomethane (3 mol/L, THAM) buffer, which can bind hydrogen proton...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Russ M,Deja M,Ott S,Bedarf J,Keckel T,Hiebl B,Wagner JJ,Unger JK

    更新日期:2011-06-01 00:00:00

  • Clinical indications for the use of the "Poisk-IOM" total artificial heart: the experience of 13 implantations in humans.

    abstract::Success of total artificial heart (TAH) implantation as a temporary measure depends on three factors: proper patient selection, adequate surgical technique, and safe cardiac prostheses. From 1986 until March 1990, 237 patients with stagnant heart failure were examined. Sixty-one patients (27%) were put on a waiting li...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Shumakov V,Zimin N,Kazakov E,Kormer A,Vorontsov S,Nikolaenko E,Kozlov I,Drobyshev A

    更新日期:1991-10-01 00:00:00

  • Long-term performance of homograft and autograft valves.

    abstract::Five hundred and eighty homograft valves have been inserted into the aortic position as isolated valve replacements, and followed up for 7--14 years. After ten years 90% of frozen homografts, 80% of autografts and 70% of freeze-dried homografts were free of valve-related death. There was only one embolism reported giv...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Bodnar E,Wain WH,Martelli V,Ross DN

    更新日期:1980-02-01 00:00:00

  • Paper is a compatible bed for rat hepatocytes.

    abstract::To develop an effective hybrid bioartificial liver (BAL) device, the material of the scaffold is very important to support hepatocytes that have both growth ability and hepatic differentiated functions. In this study we used paper (Kimwipe, Kimberly-Clark Corp., Roswell, GA, U.S.A.) as a scaffold. Primary hepatocytes ...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Mizuguchi T,Mitaka T,Sato F,Mochizuki Y,Hirata K

    更新日期:2000-04-01 00:00:00

  • How to comply with the Food and Drug Administration's "Investigational Device Exemption (IDE)" regulations including an application form (revised January 1982).

    abstract::This is a revision of the IDE Application Form, "How to Comply with the Food and Drug Administration's New 'Investigational Device Exemption (IDE)' Regulations, Including an Application Form" originally published in Artificial Organs, Volume 4, Number 4, November 1980. This publication updates the IDE application form...

    journal_title:Artificial organs

    pub_type: 杂志文章



    更新日期:1982-02-01 00:00:00

  • Usability and safety of ventricular assist devices: human factors and design aspects.

    abstract::The purpose of this study was the investigation of the usability and ergonomics of ventricular assist devices (VADs) in everyday usage. Patients with four different VAD types were observed. After implantation, instruction, and discharge from the hospital, the patients returned on a regular basis to the outpatient clin...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Geidl L,Zrunek P,Deckert Z,Zimpfer D,Sandner S,Wieselthaler G,Schima H

    更新日期:2009-09-01 00:00:00

  • A bridge from short-term to long-term left ventricular assist device--experimental verification of a physiological controller.

    abstract::A physiological controller is a key component to optimize the performance of left ventricular assist devices (LVADs) from short term to long term (10-20 years). The difficulties of its design lie in the inaccurate modeling of any particular human cardiovascular system because of limited knowledge, the large fluctuatio...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Wu Y,Allaire PE,Tao G,Adams M,Liu Y,Wood H,Olsen DB

    更新日期:2004-10-01 00:00:00

  • Antimicrobial Wound Dressing Containing Silver Sulfadiazine With High Biocompatibility: In Vitro Study.

    abstract::Many patients all over the world suffer from acute wounds caused by traumas or burns. In most crucial cases, skin regeneration cannot be promoted spontaneously, and skin grafts are applied as the main treatment. However, this therapy has some drawbacks which motivate researchers to develop wound dressings. In this stu...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Mohseni M,Shamloo A,Aghababaei Z,Vossoughi M,Moravvej H

    更新日期:2016-08-01 00:00:00

  • Bioengineered cartilage in a scaffold-free method by human cartilage-derived progenitor cells: a comparison with human adipose-derived mesenchymal stromal cells.

    abstract::The objective of our study was to investigate chondrogenesis potential of human adipose-derived mesenchymal stromal cells (MSCs), using as a positive control a human source of cartilage-derived progenitor cells (PCs). This source of PCs was recently described by our group and dwells on the surface of nasoseptal cartil...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Baptista LS,Silva KR,Pedrosa CS,Amaral RJ,Belizário JV,Borojevic R,Granjeiro JM

    更新日期:2013-12-01 00:00:00

  • Clinical Assessment of Histidine-Tryptophan-Ketoglutarate Solution and Modified St. Thomas' Solution in Pediatric Cardiac Surgery of Tetralogy of Fallot.

    abstract::The objective of this study is to compare the myocardium protective effect of Bretschneider's histidine-tryptophan-ketoglutarate (HTK) solution versus Modified St. Thomas' (STH) solution in pediatric cardiac surgery of Tetralogy of Fallot (TOF). Seventy-seven pediatric patients of TOF who received the total surgical r...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Wang ZH,An Y,Du MC,Qin TJ,Liu YB,Xu HZ,Yang LQ

    更新日期:2017-05-01 00:00:00

  • Arterial pressure: its role in perfusion and as a criterion of extracorporeal circulation adequacy.

    abstract::Thirty-five patients undergoing open-heart surgery are reported. Cardiac index, mean arterial pressure, oxygen consumption, lactate, pyruvate, and lactate excess in the blood were measured. An inverse correlation of arterial pressure with oxygen consumption and lactate excess was found during perfusion. Comparison of ...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Osipov VP,Khodas MJ,Dementjeva II,Pjatnitskaja GKh

    更新日期:1982-08-01 00:00:00

  • Separation of the natural atria in experimental implantation of total artificial heart.

    abstract::Atrial connections in a single-unit total artificial heart (TAH) may be difficult to make because of the rigidity of the device and the fixed position of the atrial inlets. We developed a technique to separate the natural atrial borders in an experimental implantation of a unitary TAH. In this technique, the interatri...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Frazier OH,Matsuwaka R,Parnis SM,Furusho N,McGee MG,Fuqua JM

    更新日期:1995-08-01 00:00:00

  • Physiologic control of cardiac assist devices.

    abstract::Total artificial hearts (TAHs) and biventricular assist devices (BVADs) have varying levels of acceptance and reliability, and the research on both focuses on their control mechanisms. Efforts generally aim to achieve a response to physiologic demand and left/right output balance, and beneficial cardiac output (CO) an...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Hall AW,Soykan O,Harken AH

    更新日期:1996-03-01 00:00:00

  • A novel, valveless ventricular assist device: the FishTail pump. First experimental in vivo studies.

    abstract::This article presents the first in vivo experiments with a new type of valveless ventricular assist device, a wave-generating pump. Our goal was to evaluate the hemodynamic performance of the pump by comparing it with the Biomedicus BP-80 centrifugal pump and by sustaining a 3 h long left ventricular assistance. We co...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Feier H,Mekkaoui C,Drevet JB,Sérée Y,Richomme C,Rolland PH,Mesana TG

    更新日期:2002-12-01 00:00:00

  • Beta-2-microglobulin and main granulocyte components in hemodialysis patients.

    abstract::Plasma levels of granulocyte elastase in complex with alpha 1-proteinase inhibitor (E-alpha 1-PI) increased during hemodialysis from 110 +/- 10 to 506 +/- 66 micrograms/L using dialyzers made of polyamide, from 95 +/- 2.2 to 211 +/- 54 micrograms/ml with hemophane and from 114 +/- 10 to 203 +/- 25 using dialyzers made...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Haag-Weber M,Schollmeyer P,Hörl WH

    更新日期:1989-04-01 00:00:00

  • Hepatocyte culture between woven capillary networks: a microscopy study.

    abstract::A multi-compartment capillary membrane culture model with independently perfused three-dimensionally woven capillaries was developed for immobilization of hepatocytes in bioreactors. This enables spatial restructuring of cells and enhanced mass transfer performance with more efficient oxygenation and metabolite exchan...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Gerlach J,Schnoy N,Smith MD,Neuhaus P

    更新日期:1994-03-01 00:00:00

  • The DeBakey ventricular assist device: current status in 1997.

    abstract::In 1993, the development began of a small axial flow blood pump, the DeBakey ventricular assist device (VAD). The material was recently converted to a titanium alloy, and a waterproof pump package was incorporated for long-term intracorporeal circulation. Thirteen intrathoracic implantations in calves were achieved. N...

    journal_title:Artificial organs

    pub_type: 临床试验,杂志文章


    authors: Tayama E,Olsen DB,Ohashi Y,Benkowski R,Morley D,Noon GP,Nosé Y,Debakey ME

    更新日期:1999-12-01 00:00:00

  • Baroreflex sensitivity of an arterial wall during rotary blood pump assistance.

    abstract::It is well known that the baroreflex system is one of the most important indicators of the pathophysiology in hypertensive patients. We can check the sensitivity of the baroreflex by observing heart rate (HR) responses; however, there is no simple diagnostic method to measure the arterial behavior in the baroreflex sy...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Yambe T,Imachi K,Shiraishi Y,Yamaguchi T,Shibata M,Kameyama T,Yoshizawa M,Sugita N

    更新日期:2009-09-01 00:00:00

  • Heart Transplant Waiting List Implications of Increased Ventricular Assist Device Use as a Bridge Strategy: A National Analysis.

    abstract:BACKGROUND:The use of ventricular assist devices (VAD) as a bridge to heart transplant (HT) is increasing, while HT volume remains stagnant. This may portend longer waiting times and an otherwise more competitive environment for all patients on the HT waiting list. METHODS:A retrospective analysis of patients who were...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Han JJ,Elzayn H,Duda MM,Iyengar A,Acker AM,Patrick WL,Helmers M,Birati EY,Atluri P

    更新日期:2020-10-01 00:00:00

  • Binding of antithrombin to immobilized heparin under varying flow conditions.

    abstract::Rheologic factors are likely to influence the balance between thrombotic and antithrombotic forces at the level of the vascular wall. In this study, the effects of flow-velocity/wall shear stress on the interaction of antithrombin (AT) with surface-immobilized heparin were investigated. The binding of AT to low-affini...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Pasche B,Elgue G,Olsson P,Riesenfeld J,Rasmuson A

    更新日期:1991-12-01 00:00:00

  • Hemodynamic evaluation of a new left ventricular assist device: an integrated cardioassist catheter as a pulsatile left ventricle-femoral artery bypass.

    abstract::We developed a new left ventricular (LV) assist catheter (transaortic valve from LV to femoral artery) that is implemented percutaneously and exerted as a synchronous pulsatile partial LV flow support with conventional intraaortic balloon pump (IABP) driving system and centrifugal pump system. We investigated whether ...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Ide H,Yamaguchi A,Ino T,Matsumoto H,Fujimasa I

    更新日期:1992-06-01 00:00:00

  • Multi-field surface electrode for selective electrical stimulation.

    abstract::We designed a 24-field array and an on-line control box that selects which and how many of 24 fields will conduct electrical charge during functional electrical stimulation. The array was made using a conductive microfiber textile, silver two-component adhesive, and the conductive ink imprint on the polycarbonate. The...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Popović-Bijelić A,Bijelić G,Jorgovanović N,Bojanić D,Popović MB,Popović DB

    更新日期:2005-06-01 00:00:00

  • What Is the Optimal Setting for a Continuous-Flow Left Ventricular Assist Device in Severe Mitral Regurgitation?

    abstract::Excessive left ventricular (LV) volume unloading can affect right ventricular (RV) function by causing a leftward shift of the interventricular septum in patients with mitral regurgitation (MR) receiving left ventricular assist device (LVAD) support. Optimal settings for the LVAD should be chosen to appropriately cont...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Naito N,Nishimura T,Takewa Y,Kishimoto S,Date K,Umeki A,Ando M,Ono M,Tatsumi E

    更新日期:2016-11-01 00:00:00

  • Cytotoxicity of bile in human Hep G2 cells and in primary cultures of rat hepatocytes.

    abstract::There has been increasing interest in the development of a hepatocyte bioreactor for the treatment of acute hepatic failure; however, little is known about the effect of hepatocyte byproducts on the viability of the cells in the bioreactor environment. We investigated the effects of increasing concentrations of bile o...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Smirthwaite AD,Gaylor JD,Cousins RB,Grant MH

    更新日期:1998-10-01 00:00:00

  • Phosphorylcholine and poly(D,L-lactic acid) containing copolymers as substrates for cell adhesion.

    abstract::Fibroblast cell culture was performed to evaluate cell adhesion and cell morphology on novel hydrolyzable copolymers composed of poly(D,L-lactic acid) (PDLA) macromonomer, 2-methacryloyloxyethyl phosphorylcholine (MPC), and n-butyl methacrylate. The copolymers were used as cell culture materials for regulating the int...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Watanabe J,Ishihara K

    更新日期:2003-03-01 00:00:00

  • Prevention of right heart failure after left ventricular assist device implantation by phosphodiesterase 5 inhibitor.

    abstract::Right ventricular (RV) function immediately after left ventricular assist device (LVAD) implantation is a crucial prognostic factor. RV failure is linked to increased mortality and worse outcome. A phosphodiesterase 5 inhibitor, sildenafil, was shown to decrease pulmonary vascular resistance and pulmonary artery press...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Hamdan R,Mansour H,Nassar P,Saab M

    更新日期:2014-11-01 00:00:00

  • Kinetics of group IB and IIA phospholipase A2 during low-volume continuous hemodiafiltration in severe acute pancreatitis.

    abstract::Continuous hemodiafiltration (CHDF) has been performed for the treatment of severe acute pancreatitis. Phospholipase A2 (PLA2) is one of the important mediators which exacerbate acute pancreatitis, but whether PLA2 can be removed by CHDF is unclear. In this study, the kinetics of group IB and group IIA PLA2 was examin...

    journal_title:Artificial organs

    pub_type: 临床试验,杂志文章


    authors: Suzuki M,Okahisa T,Sogabe M,Iwaki H,Okita Y,Ohnishi Y,Ito S

    更新日期:2007-05-01 00:00:00

  • Stem damage during implantation of modular hip prostheses.

    abstract::Modular hip prostheses are commonly used today. Dedicated surgical auxiliary instrumentation is indispensable. A correct coupling between the prosthesis and the instrument is necessary to avoid damage. Based on a specific commercial design (AnCAFit, Wright Cremascoli Ortho, Toulon, France), the aim of this work was to...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Varini E,Cristofolini L,Viceconti M,Traina F

    更新日期:2006-07-01 00:00:00

  • The impact of pump speed and inlet cannulation site on left ventricular unloading with a rotary blood pump.

    abstract:BACKGROUND:Ventricular assist devices are gaining ground in the therapeutic treatment of chronic heart failure. These devices are sometimes used as a bridge to recovery by unloading the left ventricle (LV) and restoring its function. It is therefore important to preserve the heart muscle and apply less invasive implant...

    journal_title:Artificial organs

    pub_type: 杂志文章


    authors: Vandenberghe S,Nishida T,Segers P,Meyns B,Verdonck P

    更新日期:2004-07-01 00:00:00

  • Adverse events of subcutaneous recombinant human erythropoietin therapy: results of a controlled multicenter European study.

    abstract::In a controlled European multicenter study, clinical tolerance of subcutaneously administered recombinant human erythropoietin (rh-EPO) therapy and its influence on the course of illness in 362 hemodialyzed patients (162 males, 200 females) from 16 European dialysis centers was studied. Of these, 181 patients served a...

    journal_title:Artificial organs

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Klinkmann H,Wieczorek L,Scigalla P

    更新日期:1993-04-01 00:00:00