Abstract:
:The limited success of cardiac stem cell therapy has lately generated discussion regarding its effectiveness. We hypothesized that immediate cell loss after intramyocardial injection significantly obscures the regenerative potential of stem cell therapy. Therefore, our aim was to assess the distribution and quantity of induced pluripotent stem cells after intramyocardial delivery using in vivo bioluminescence analysis. In this context, we wanted to investigate if the injection of different cell concentrations would exert influence on cardiac cell retention. Murine-induced pluripotent stem cells were transfected for luciferase reporter gene expression and transplanted into infarcted myocardium in mice after left anterior descending coronary artery ligation. Cells were delivered constantly in aqueous media (15 μL) in different cell concentrations (group A, n = 10, 5.0 × 10(5) cells; group B, n = 10, 1.0 × 10(6) cells). Grafts were detected using bioluminescence imaging. Organ explants were imaged 10 min after injection to quantify early cardiac retention and cell biodistribution. Bioluminescence imaging showed a massive early displacement from the injection site to the pulmonary circulation, leading to lung accumulation. Mean cell counts of explanted organs in group A were 7.51 × 10(4) ± 4.09 × 10(3) (heart), 6.44 × 10(4) ± 2.48 × 10(3) (left lung), and 8.06 × 10(5) ± 3.61 × 10(3) (right lung). Respective cell counts in group B explants were 1.69 × 10(5) ± 7.69 × 10(4) (heart), 2.11 × 10(5) ± 4.58 × 10(3) (left lung), and 3.25 × 10(5) ± 9.35 × 10(3) (right lung). Applying bioluminescence imaging, we could unveil and quantify massive early cardiac stem cell loss and pulmonary cell accumulation following intramyocardial injection. Increased injection concentrations led to much higher intracardiac cell counts; however, pulmonary biodistribution of transplanted cells still persisted. Therefore, we recommend applying tissue engineering techniques for cardiac stem cell transplantations in order to improve cardiac retention and limit biodistribution.
journal_name
Artif Organsjournal_title
Artificial organsauthors
Martens A,Rojas SV,Baraki H,Rathert C,Schecker N,Zweigerdt R,Schwanke K,Rojas-Hernandez S,Martin U,Saito S,Schmitto JD,Haverich A,Kutschka Idoi
10.1111/aor.12268subject
Has Abstractpub_date
2014-11-01 00:00:00pages
978-84issue
11eissn
0160-564Xissn
1525-1594journal_volume
38pub_type
杂志文章abstract::An important present and emerging trend in biomaterials development is the synthesis of polymers which combine capabilities of biologic recognition ("biomimetic") with sensitivity to small environmental changes ("stimuli-responsive"). This short article highlights some of the many opportunities and challenges in this ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1988.tb02813.x
更新日期:1988-12-01 00:00:00
abstract::The heparin-conjugated fibrin (HCF) system has been developed to deliver bone morphogenetic proteins (BMPs) for a long-term period and thus enhance bone regeneration. In the present study, we tested the effectiveness of the delivery system for spinal fusion with a very low dose of BMP-2. A total of 15 rabbits underwen...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2012.01578.x
更新日期:2013-05-01 00:00:00
abstract::An undulation pump total artificial heart (UPTAH) in which the revolutions of the motor are converted to undulation motion of a disk has been developed. In an experiment, a goat using the UPTAH survived for 54 days. However, a large ripple was observed in the device's output pressure and flow waveform. In calculating ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2002.06798.x
更新日期:2002-01-01 00:00:00
abstract::A bypass flow rate estimation and control system (BECS) for an implantable centrifugal blood pump (ICBP) has been developed in our institute. The estimated flow rate (EF) of the ICBP was derived from the electric power consumption, the rotating speed of a motor, and the blood viscosity presumed by the hematocrit and b...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.1998.06130.x
更新日期:1998-06-01 00:00:00
abstract::Technological innovations in pediatric extracorporeal life support circuits can reduce system-related complications and may improve patients' outcome. The Deltastream DP3 (Medos Medizintechnik AG, Stolberg, Germany) is a novel rotational pump with a diagonally streamed impeller that can be used over a broad range of f...
journal_title:Artificial organs
pub_type: 杂志文章,多中心研究
doi:10.1111/aor.13016
更新日期:2018-04-01 00:00:00
abstract::This work introduces a new Lagrangian strain-based model to predict the shear-induced hemolysis in biomedical devices. Current computational models for device-induced hemolysis usually utilize empirical fitting of the released free hemoglobin (Hb) in plasma from damaged red blood cells (RBCs). These empirical correlat...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.13663
更新日期:2020-08-01 00:00:00
abstract::The aim of this study was to evaluate the safety and performance of the Arrow CorAide left ventricular assist system (LVAS) (Arrow International, Reading, PA, USA), a continuous-flow left ventricular assist device, as bridge to transplantation or recovery as well as destination therapy in patients with New York Heart ...
journal_title:Artificial organs
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1111/j.1525-1594.2012.01539.x
更新日期:2013-02-01 00:00:00
abstract::The specific timing for discontinuing renal replacement therapy (RRT) in acute kidney injury (AKI) patients is debatable. The predictive abilities of variables at the time of discontinuation of RRT for the long-term prognoses of patients have not been explored. This study aimed to explore the prognostic factors upon d...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12927
更新日期:2017-12-01 00:00:00
abstract::The sensitivity of liver allografts to even minimal periods of ischemia currently limits the duration of hepatic preservation prior to liver transplantation. This study evaluates the role of activated carbon hemoperfusion (ACH) for assisting the recovery of canine livers ischemically damaged by a 20-min occlusion of t...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1985.tb04386.x
更新日期:1985-08-01 00:00:00
abstract::To provide percutaneous access, a new circulatory assist system was developed. We call this newly developed system the modified assist device (MAD). The system is composed of a sac-type blood pump and cannula. Inflow and outflow valves are mounted in the apex and at the side wall 10 cm from the apex of the cannula, re...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1996.tb00718.x
更新日期:1996-02-01 00:00:00
abstract::The cochlea is an end organ, which is metabolically dependent on a nutrient and oxygen supply to maintain its normal physiological function. Cochlear ischemia and reperfusion (IR) injury is considered one of the most important causes of human idiopathic sudden sensorineural hearing loss. The aim of the present study w...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12863
更新日期:2017-08-01 00:00:00
abstract::We carried out this retrospective study to examine the magnesium status of our chronic ambulatory peritoneal dialysis (CAPD) patients dialyzed with 0.75 mmol/L (group I) or 0.50 mmol/L (group II) magnesium peritoneal dialysis solution. A total of 34 anuric patients on CAPD (age:31-72 years; duration of CAPD:7-74 month...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2003.07193.x
更新日期:2003-09-01 00:00:00
abstract::The injurious effects of reactive oxygen species on venous tissues and the potential protective role played by green tea polyphenol (GTPP) on human saphenous veins were investigated. Oxidative stress was induced exogenously in the vein segments, either by adding 0.8 or 1.6 M of H2O2, or by using 80 or 160 U/L of xanth...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2003.07163.x
更新日期:2003-12-01 00:00:00
abstract::Spiral/helical forms of blood flow have been observed in large arteries of the cardiovascular system, but their benefits remain underappreciated. Spiral flow has been postulated to improve near-wall washout, promoting anti-atherothrombotic conditions. This research aims to study the washout characteristics of spiral f...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.13520
更新日期:2019-12-01 00:00:00
abstract::In a controlled European multicenter study, clinical tolerance of subcutaneously administered recombinant human erythropoietin (rh-EPO) therapy and its influence on the course of illness in 362 hemodialyzed patients (162 males, 200 females) from 16 European dialysis centers was studied. Of these, 181 patients served a...
journal_title:Artificial organs
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1111/j.1525-1594.1993.tb00571.x
更新日期:1993-04-01 00:00:00
abstract::A small and light direct-drive centrifugal pump has been developed for cardiopulmonary bypass. In the development process, blood compatibility studies including a hemolysis study, an in vitro fluid dynamic performance study, and in vivo durability and feasibility studies were performed. The centrifugal pump with a 50 ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1992.tb00558.x
更新日期:1992-12-01 00:00:00
abstract::ABO incompatible allogeneic bone marrow transplants can be performed successfully to treat patients with leukemia or aplastic anemia. These transplants carry no great risk of rejection or graft-versus-host disease, however, some method must be used to avoid acute hemolysis at the time of infusion of ABO incompatible m...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1981.tb03998.x
更新日期:1981-08-01 00:00:00
abstract::Controlled and repeatable in vitro evaluation of cardiovascular devices using a mock circulation loop (MCL) is essential prior to in vivo or clinical trials. MCLs often consist of only a systemic circulation with no autoregulatory responses and limited validation. This study aimed to develop, and validate against huma...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.13636
更新日期:2020-06-01 00:00:00
abstract::In clinical settings, no method has been established to examine the fatigue of a latissimus dorsi muscle (LDM) preconditioned for cardiomyoplasty. We examined the feasibility of measuring muscle stiffness (tactile stiffness) to evaluate muscle fatigue in situ using our tactile sensor. We stimulated canine LDM with bur...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2000.06551.x
更新日期:2000-10-01 00:00:00
abstract::Turbulent blood flow in medical devices contributes to blood trauma, yet the exact mechanism(s) have not been fully elucidated. Local turbulent stresses, viscous stresses, and the rate of dissipation of the turbulent kinetic energy have been proffered as hypotheses to describe and predict blood damage. In this work, s...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12572
更新日期:2015-12-01 00:00:00
abstract::Eight patients were studied during four sessions of acetate-free biofiltration (AFBF). AFBF is a new dialysis technique with no base replacement agents in the dialysate and with the addition in postdilution mode of bicarbonate (HCO3) solution directly into the extracorporeal blood circuit. In this study the effects on...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1989.tb01561.x
更新日期:1989-10-01 00:00:00
abstract::Five typical mechanical heart valves (Starr-Edwards, Björk-Shiley convexo-concave (c-c), Björk-Shiley monostrut, Bicer-Val, and St. Jude Medical) were tested in the mitral position under the pulsatile flow condition. The test program included measurements of velocity and turbulent stresses at 5 downstream locations. T...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1997.tb00451.x
更新日期:1997-10-01 00:00:00
abstract::To obtain an optimal design of the left blood chamber of the total artificial heart (TAH), flow visualization studies were performed. Information on velocities in critical areas of the left chamber was gathered using sectional pulsed laser light. The flow patterns on the entire pumping duration were photographed frame...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1994.tb03345.x
更新日期:1994-12-01 00:00:00
abstract::A new intravascular pumping oxygenator (IVPO) was developed for intravascular gas exchange and circulatory assistance in critically ill patients with respiratory and circulatory failure. The IVPO utilizes new silicone hollow fibers (diameter, 1 mm; membrane width, 50 microns) and consists of two driving tubes for the ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1997.tb00703.x
更新日期:1997-01-01 00:00:00
abstract::Tissue-engineered heart valves constructed from a xenogeneic or allogeneic decellularized matrix might overcome the disadvantages of current heart valve substitutes. One major necessity besides effective decellularization is to preserve the biomechanical properties of the valve. Native and decellularized porcine pulmo...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2007.00452.x
更新日期:2008-01-01 00:00:00
abstract::Early in 1956, the first model of a biological artificial liver, using a live dog's liver incorporated in a cross-hemodialyzer, was placed in an experimental animal with portocaval encephalopathy. This "biological artificial liver," a hybrid artificial liver in the present terminology, was the first in the world. In O...
journal_title:Artificial organs
pub_type: 历史文章,杂志文章
doi:10.1111/j.1525-1594.1986.tb02548.x
更新日期:1986-06-01 00:00:00
abstract::The thrombogenicity of membrane oxygenators as well as clotting parameters profiles, using standard human clotting tests, was analyzed in calves and pigs during 6 h perfusion. Three calves and 3 pigs were connected to extracorporeal circulation with standard heparinization. Blood samples were taken for coagulation var...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2001.025007579.x
更新日期:2001-07-01 00:00:00
abstract::The combination of plasma exchange and continuous hemofiltration was applied to patients with acute liver failure, and its clinically advantageous effects were evaluated. This procedure was found to be a powerful approach in decreasing patient morbidity; however, the effect was temporary and the patients finally died....
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1994.tb02217.x
更新日期:1994-05-01 00:00:00
abstract::The use of extracorporeal membrane oxygenator instead of standard cardiopulmonary bypass during lung transplantation is debatable. Moreover, recently, the concept of prolonged postoperative extracorporeal membrane oxygenator (ECMO) support has been introduced in many transplant centers to prevent primary graft dysfunc...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.13628
更新日期:2020-06-01 00:00:00
abstract::Transcatheter aortic valve implantation (TAVI) has gained considerable acceptance in the past decade due to its lower risks than conventional open-heart surgery. However, the deformation and delamination of the leaflets during the crimping procedure have raised questions about the durability and long-term serviceabili...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.13503
更新日期:2019-10-01 00:00:00