Abstract:
:Transcorneal drug transport is normally limited by the intrinsic permeation characteristics of the corneal epithelium. However, liposomes, i.e., phospholipid vesicles composed of phospholipid membranes, have recently attracted attention as carriers of topically applied agents. The present study therefore describes a qualitative and quantitative laboratory investigation of interactions between corneal epithelial cells and liposomes. The lipid bilayers and interior spaces of liposomes were labelled with different fluorophores. Fluorescence microscopy revealed a rapid uptake of rhodamine B-labelled liposome bilayer components by the epithelial cell membrane and the cytoplasm. Simultaneously, intracellular uptake of aqueous liposome content was indicated by uniform fluorescence of the cytoplasm due to carboxyfluorescein (CF). The fluorimetric experiments showed that the uptake of liposomes by SIRC cells depended on liposome concentrations and the time of exposure of the cells to the liposomes, and that saturation effect characteristics were present. Cell fluorescence dropped by approximately 45% when the incubation temperature of the cells was reduced from 37 degrees C to 4 degrees C. Both this phenomenon and a significant reduction in liposome uptake (p < 0.05 and p < 0.01) after incubation with the metabolic inhibitors 2-deoxyglucose and sodium azide indicated active, energy-dependent processes. Phagocytosis in cell-liposome interactions was directly shown by a significant reduction in cell fluorescence (p < 0.05) after application of the actin inhibitor cytochalasin B. The results presented here give concrete data on interactions between liposomes and superficial cells of the eye in vitro.
journal_name
Surv Ophthalmoljournal_title
Survey of ophthalmologyauthors
Pleyer U,Grammer J,Kosmidis P,Ruckert DGdoi
10.1016/s0039-6257(05)80068-2subject
Has Abstractpub_date
1995-05-01 00:00:00pages
S3-16eissn
0039-6257issn
1879-3304pii
S0039-6257(05)80068-2journal_volume
39 Suppl 1pub_type
杂志文章abstract::Recent literature suggests that the incidence of postoperative endophthalmitis is variable and may be on the rise. This article reviews established perioperative preventative measures, and discusses some possible causes of postoperative endophthalmitis that may be insufficiently addressed by current risk reduction tec...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/j.survophthal.2004.01.002
更新日期:2004-03-01 00:00:00
abstract::With an occurrence of approximately 1 in 3000 births, von Recklinghausen neurofibromatosis (NF) is one of the most common inherited human disorders. NF is considered a neurocristopathy, a disorder of neural crest derived cells. One of the complications of NF is the development of neural crest derived malignancies such...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/0039-6257(89)90014-3
更新日期:1989-03-01 00:00:00
abstract::For 11 years, a 50-year-old woman with euthyroid Graves' disease experienced intermittent exacerbations of her orbitopathy associated with a decline in visual acuity. On each occasion, treatment with systemic corticosteroids led to prompt recovery of vision. Upon referral for consideration for orbital decompressive su...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0039-6257(97)00094-5
更新日期:1997-11-01 00:00:00
abstract::Unknown to the Western hemisphere, a Polish scientist, naturalist, anatomist, physiologist, philosopher and optician, Witelo, wrote an important ten volume manuscript, Peri-Optikes, usually cited in the literature as Perspectiva. This classic textbook was first published in 1535 in Nuremberg under the title: Vitellion...
journal_title:Survey of ophthalmology
pub_type: 传,历史文章,杂志文章
doi:10.1016/s0039-6257(96)80028-2
更新日期:1996-11-01 00:00:00
abstract::The abundant data about retinal tears has shown that they are prevalent in the general population and that the vast majority of retinal tears do not lead to retinal detachment. What remains to be clarified are the criteria for identifying the few retinal breaks which require prophylactic surgery to prevent their progr...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/0039-6257(80)90149-6
更新日期:1980-09-01 00:00:00
abstract:OBJECTIVE:Laser photocoagulation treatment of the posterior pole of the retina is often complicated by immediate visual impairment, which is caused by the unavoidable laser-induced destruction of the normal tissue adjacent to the lesion. A neuroprotective therapy aimed at salvaging this normal tissue might enhance the ...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0039-6257(99)00093-4
更新日期:1999-10-01 00:00:00
abstract::Solitary fibrous tumor of the orbit is a rare spindle cell neoplasm. There are 42 cases of solitary fibrous tumor of the orbit available in the literature. We present six more cases of orbital solitary fibrous tumors, which presented to our institute between 1999 and 2001. We highlight the need for clinical recognitio...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/s0039-6257(03)00087-0
更新日期:2003-09-01 00:00:00
abstract::Eye cancer-related glaucomas occur through a variety of mechanisms. They can be challenging to diagnose and are often refractory to treatment. The literature reveals the variety of ways eye cancers cause glaucoma. Mechanisms include direct invasion, infiltration, or seeding of the aqueous outflow structures as well as...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/j.survophthal.2008.10.002
更新日期:2009-01-01 00:00:00
abstract::Early diagnosis of glaucoma is critical to prevent permanent structural damage and irreversible vision loss. Detection of glaucoma typically relies on examination of structural damage to the optic nerve combined with measurements of visual function. To aid the clinician in evaluation of visual function and structure, ...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/j.survophthal.2008.08.003
更新日期:2008-11-01 00:00:00
abstract::Latent herpes simplex virus (HSV) is known to reside in the trigeminal ganglia. Our studies show that the temporary retrobulbar disruption of trigeminal nerve function in chronically infected animals caused a striking decrease in the number of positive HSV cultures obtained during the 20 weeks immediately following su...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/0039-6257(76)90098-9
更新日期:1976-09-01 00:00:00
abstract::A 30-year-old man presented with painless total visual loss in the right eye. Fundus exam and imaging was consistent with total internal carotid artery occlusion and hemispheric stroke. The presentation and management options of retinal vascular occlusive disease in association with asymptomatic stroke are discussed. ...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.survophthal.2014.06.007
更新日期:2015-05-01 00:00:00
abstract::Glutamatergic excitotoxicity has been implicated as a mechanism for injury in a variety of central nervous system pathologies, including glaucoma. Memantine, an NMDA-type glutamatergic open-channel blocker, has pharmacologic properties that make its efficacy greater under excitotoxic conditions, but lesser under norma...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0039-6257(01)00200-4
更新日期:2001-05-01 00:00:00
abstract::Ab interno trabeculectomy with the trabectome is a microincisional glaucoma surgery modality with an electrosurgical device to ablate the trabecular meshwork and inner wall of Schlemm canal to restore conventional outflow along its physiological route, thereby decreasing intraocular pressure. The device was approved b...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/j.survophthal.2016.03.012
更新日期:2017-01-01 00:00:00
abstract::The purpose of the article is to describe the optics of the contact wide-angle lens system with stereo-reinverter for vitreous surgery. A panoramic viewing system is made up of two components; an indirect ophthalmoscopy lens system for fundus image viewing, which is placed on the patient's cornea as a contact lens, an...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/j.survophthal.2004.04.010
更新日期:2004-07-01 00:00:00
abstract::A 46-year-old extremely obese black woman presented with headaches, blurred vision, and visual obscurations. Her exam was notable for bilateral severe papilledema, retinal hemorrhages, and lethargy. Her CAT scan was normal, and a spinal tap revealed a very high opening pressure. Although this patient's presentation mi...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0039-6257(05)80108-0
更新日期:1995-01-01 00:00:00
abstract::To compare the cost-effectiveness of bimatoprost versus latanoprost for the treatment of glaucoma and ocular hypertension, a cost-effectiveness algorithm was developed to estimate the annual costs and cost per treatment success (cost-effectiveness) for bimatoprost 0.03% QD compared with latanoprost 0.005% QD. Medicati...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.survophthal.2003.12.018
更新日期:2004-03-01 00:00:00
abstract::The cornea's intense innervation is responsible for corneal trophism and ocular surface hemostasis maintenance. Corneal diabetic neuropathy affects subbasal nerve plexus, with progressive alteration of nerves' morphology and density. The quantitative analysis of nerve fibers can be performed with in vivo corneal confo...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/j.survophthal.2020.09.003
更新日期:2020-09-19 00:00:00
abstract::Through the efforts of Dr. Townley Paton, eye banks were founded in New York in the early 1940s. Since that time there has been steady growth in both the supply of and demand for donor material. A number of factors have contributed to the success of eye banks in the U.S.A., including improvements in eye banking techni...
journal_title:Survey of ophthalmology
pub_type: 历史文章,杂志文章
doi:10.1016/0039-6257(82)90151-5
更新日期:1989-01-01 00:00:00
abstract::Testicular lymphoma is a rare neoplasm of the testis that is most commonly seen in older patients. It metastasizes preferentially to extranodal sites, including the skin, central nervous system, Waldeyer ring, contralateral testis, and lung. Two case reports of patients with a history of testicular lymphoma who develo...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.survophthal.2005.11.002
更新日期:2006-01-01 00:00:00
abstract::There are numerous unresolved issues and controversies regarding the management of open-globe injuries involving the posterior segment. These areas include, but are not limited to, the following issues. Although vitrectomy has been shown to improve visual outcomes and allow retention of the eye in many cases, the exte...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/s0039-6257(99)00104-6
更新日期:1999-11-01 00:00:00
abstract::A 56-year-old woman presented with a unilateral ptosis induced by a nonembedded soft contact lens of approximately 2 years' duration. The unilateral ptosis most likely resulted form localized inflammation and the physical presence of the soft contact lens. The patient's symptoms resolved completely after double lid ev...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0039-6257(98)00029-0
更新日期:1998-09-01 00:00:00
abstract::Based on their mechanism of action, the most frequently used ocular hypertensive agents, the beta-blockers, cannot be assumed to reduce IOP during sleep. The need for drugs that reduce IOP around-the-clock is underscored, however, by the fact that inadequate nocturnal ocular perfusion pressure is considered to be one ...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/s0039-6257(97)80021-5
更新日期:1997-02-01 00:00:00
abstract::Autosomal dominant optic atrophy is an inherited optic neuropathy manifesting with variable penetrance and expressivity. Other genetic and environmental factors are postulated to contribute to more marked visual loss in some affected individuals. Optic neuropathy is also a known adverse effect of ethambutol therapy fo...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.survophthal.2009.10.005
更新日期:2010-07-01 00:00:00
abstract::Retinoblastoma usually presents in children younger than 5 years. To our knowledge, 20 cases of retinoblastoma in adults (older than 20 years) have been reported in the literature. Of 173 patients with histopathologically proven retinoblastoma seen in our institute, three were adults. All the patients had endophytic t...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/s0039-6257(99)00132-0
更新日期:2000-03-01 00:00:00
abstract::The definition of glaucoma has changed over the decades from a simple ocular pressure disease to a systemic disorder of multivariate etiology. Glaucoma may be defined for the individual eye as a chronic ocular disease with various underlying pathophysiologic disorders. However, elevated intraocular pressure (IOP) is s...
journal_title:Survey of ophthalmology
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.survophthal.2008.08.024
更新日期:2008-11-01 00:00:00
abstract::In the current medical environment, long-standing and appropriate referral patterns among medical care providers may be scrutinized for possible violation of a technical provision of Medicare, the "anti-fraud and abuse statute." The physician can avoid misunderstanding by preparing and implementing a compliance progra...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/s0039-6257(99)90134-0
更新日期:2000-05-01 00:00:00
abstract::Once we understand that an increase in the size of the optic disk cup is due to loss of optic nerve fibers combined with some physical tissue rearrangements, it is quite clear that cupping begins as soon as nerve loss begins. Methods to detect cupping are more sensitive to the earliest glaucoma damage than are present...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/0039-6257(85)90080-3
更新日期:1985-09-01 00:00:00
abstract::Recent research on classical red-green blind observers has shown that complete dichromacy may be present only under conditions where the viewing angle is small. For viewing angles greater than about 4 degrees, both rods and an anomalous cone have been shown to underlie a weak form of trichromacy. The conditions for ro...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/0039-6257(83)90230-8
更新日期:1983-03-01 00:00:00
abstract::Cortical blindness is an uncommon, but dramatic, complication of preeclampsia. We present a case in which diffusion-weighted magnetic resonance imaging played a critical role in determining the etiology of the cortical blindness and its subsequent management. ...
journal_title:Survey of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.survophthal.2003.08.008
更新日期:2003-11-01 00:00:00
abstract::The clinical and histopathologic features of Axenfeld's anomaly and Rieger's anomaly and syndrome are reviewed, and recent findings regarding the pathogenesis of this spectrum of developmental disorders are discussed. Based on these observations, it has been suggested that a developmental arrest, in the third trimeste...
journal_title:Survey of ophthalmology
pub_type: 杂志文章,评审
doi:10.1016/0039-6257(85)90205-x
更新日期:1985-05-01 00:00:00