Analysis of interactions between the corneal epithelium and liposomes: qualitative and quantitative fluorescence studies of a corneal epithelial cell line.


:Transcorneal drug transport is normally limited by the intrinsic permeation characteristics of the corneal epithelium. However, liposomes, i.e., phospholipid vesicles composed of phospholipid membranes, have recently attracted attention as carriers of topically applied agents. The present study therefore describes a qualitative and quantitative laboratory investigation of interactions between corneal epithelial cells and liposomes. The lipid bilayers and interior spaces of liposomes were labelled with different fluorophores. Fluorescence microscopy revealed a rapid uptake of rhodamine B-labelled liposome bilayer components by the epithelial cell membrane and the cytoplasm. Simultaneously, intracellular uptake of aqueous liposome content was indicated by uniform fluorescence of the cytoplasm due to carboxyfluorescein (CF). The fluorimetric experiments showed that the uptake of liposomes by SIRC cells depended on liposome concentrations and the time of exposure of the cells to the liposomes, and that saturation effect characteristics were present. Cell fluorescence dropped by approximately 45% when the incubation temperature of the cells was reduced from 37 degrees C to 4 degrees C. Both this phenomenon and a significant reduction in liposome uptake (p < 0.05 and p < 0.01) after incubation with the metabolic inhibitors 2-deoxyglucose and sodium azide indicated active, energy-dependent processes. Phagocytosis in cell-liposome interactions was directly shown by a significant reduction in cell fluorescence (p < 0.05) after application of the actin inhibitor cytochalasin B. The results presented here give concrete data on interactions between liposomes and superficial cells of the eye in vitro.


Surv Ophthalmol


Survey of ophthalmology


Pleyer U,Grammer J,Kosmidis P,Ruckert DG




Has Abstract


1995-05-01 00:00:00










39 Suppl 1


  • Malignant rhabdoid transformation of a longstanding, aggressive, and recurrent orbital angiomyxoma.

    abstract::A 47-year-old woman presented with a medial orbital tumor initially diagnosed as either a myxoid neurofibroma or myoepithelioma. Over 30 years the tumor recurred seven times and was serially debulked. Careful histopathologic analysis coupled with immunohistochemical studies performed on the last two biopsies establish...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Jakobiec FA,Callahan AB,Stagner AM,Lee NG,Rashid A,Mendoza P,Grove A,Freitag SK

    更新日期:2015-03-01 00:00:00

  • Caspar Stromayr: sixteenth century ophthalmologist.

    abstract::Caspar Stromayr, ophthalmologist and hernia surgeon, is credited with writing the first German ophthalmic work of known authorship. Written in about 1559 as an appendix to Practica Copiosa, a larger work on hernia surgery, the ophthalmic chapters describe Stromayr's thoughts on the etiology and treatment of cataract. ...

    journal_title:Survey of ophthalmology

    pub_type: 传,历史文章,杂志文章


    authors: Blanchard DL

    更新日期:1990-09-01 00:00:00

  • Corneal preservation: recent advances.

    abstract::New methods of corneal preservation for penetrating keratoplasty have recently been developed to meet the increasing demand for donor corneal tissue. Techniques utilized in the laboratory to evaluate the viability of the corneal endothelium and the clinical results obtained with each method are discussed. All of the n...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章


    authors: Van Horn DL,Schultz RO

    更新日期:1977-01-01 00:00:00

  • Pathology of human cystoid macular edema.

    abstract::The light and electron microscopic findings are reviewed in two patients who had eyes enucleated for peripheral choroidal malignant melanomas. Preoperatively, cystoid macular edema was documented by fluorescein angiography in the melanoma-containing eye in both patients. Intracytoplasmic swelling (edema) of the Müller...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章


    authors: Yanoff M,Fine BS,Brucker AJ,Eagle RC Jr

    更新日期:1984-05-01 00:00:00

  • Early detection of glaucomatous damage. II. Changes in the appearance of the optic disk.

    abstract::Once we understand that an increase in the size of the optic disk cup is due to loss of optic nerve fibers combined with some physical tissue rearrangements, it is quite clear that cupping begins as soon as nerve loss begins. Methods to detect cupping are more sensitive to the earliest glaucoma damage than are present...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章


    authors: Quigley HA

    更新日期:1985-09-01 00:00:00

  • Caught by a masquerade: sclerosing orbital inflammation.

    abstract::Idiopathic sclerosing inflammation of the orbit is a distinct form of orbital inflammatory disease characterized by slow and relentless involvement of orbital structures. It is this insidious and relentless course that makes distinction from neoplastic lesions clinically difficult. We report the case of a patient with...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章


    authors: Thorne JE,Volpe NJ,Wulc AE,Galetta SL

    更新日期:2002-01-01 00:00:00

  • ExPRESS shunt surgery: preferred glaucoma surgery in residency training?

    abstract::It is important to equip future ophthalmic surgeons with appropriate surgical skills without compromising patient care. The ExPRESS implant surgery is very similar to trabeculectomy surgery in both efficacy and technique, but is less traumatic and may be prone to less postoperative hypotony and require less postoperat...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章


    authors: Freidl KB,Moster MR

    更新日期:2012-07-01 00:00:00

  • Orbital lymphoma associated with acquired immune deficiency syndrome (AIDS).

    abstract::A new case of AIDS-associated, small noncleaved cell (Burkitt's) lymphoma that presented as an orbital mass is described. Extraocular muscle involvement was documented by computed tomography and confirmed by orbital biopsy. Extensive abdominal involvement was subsequently diagnosed and this caused the patient's death ...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Reifler DM,Warzynski MJ,Blount WR,Graham DM,Mills KA

    更新日期:1994-01-01 00:00:00

  • The invention and early manufacture of bifocals.

    abstract::Benjamin Franklin, who died 200 years ago, is generally credited with the invention of bifocals. However, serious historians have from time to time produced evidence to suggest that others may have preceded him in the invention. This historical article includes correspondence from George Whatley, a London philanthropi...

    journal_title:Survey of ophthalmology

    pub_type: 传,历史文章,杂志文章


    authors: Letocha CE

    更新日期:1990-11-01 00:00:00

  • The application of optical coherence tomography angiography in retinal diseases.

    abstract::Optical coherence tomography angiography (OCTA) is a new, noninvasive imaging technique that generates real-time volumetric data on chorioretinal vasculature and its flow pattern. With the advent of high-speed optical coherence tomography, established enface chorioretinal segmentation, and efficient algorithms, OCTA g...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Sambhav K,Grover S,Chalam KV

    更新日期:2017-01-01 00:00:00

  • Methylprednisolone therapy for retinal laser injury.

    abstract:OBJECTIVE:Laser photocoagulation treatment of the posterior pole of the retina is often complicated by immediate visual impairment, which is caused by the unavoidable laser-induced destruction of the normal tissue adjacent to the lesion. A neuroprotective therapy aimed at salvaging this normal tissue might enhance the ...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章


    authors: Solberg Y,Dubinski G,Tchirkov M,Belkin M,Rosner M

    更新日期:1999-10-01 00:00:00

  • Intraoperative and postoperative pain in cataract surgery.

    abstract::Technologic improvements in cataract surgery have not only improved visual outcomes, but also have minimized intraoperative and postoperative pain. We review the mechanisms, risk factors, and management of intraoperative and postoperative pain. Summaries of less common sources of physiologic pain have been included to...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Assam JH,Bernhisel A,Lin A

    更新日期:2018-01-01 00:00:00

  • Antibiotic prophylaxis in cataract surgery in the setting of penicillin allergy: A decision-making algorithm.

    abstract::Cataract surgery is the most commonly performed surgical procedure in many developed countries. Postoperative endophthalmitis is a rare complication with potentially devastating visual outcomes. Currently, there is no global consensus regarding antibiotic prophylaxis in cataract surgery despite growing evidence of the...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: LaHood BR,Andrew NH,Goggin M

    更新日期:2017-01-01 00:00:00

  • Intraocular lens confusions: a preventable "never event" - The Royal Victorian Eye and Ear Hospital protocol.

    abstract::Intraocular lens (IOL) confusions and errors are among the most common postoperative adverse events. Errors may occur at any stage from the decision to operate to the insertion of the IOL. The most common errors occur during IOL selection pre-operative preparation (anaesthesia given before recognition that the intende...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Zamir E,Beresova-Creese K,Miln L

    更新日期:2012-09-01 00:00:00

  • Efficacy and safety of memantine, an NMDA-type open-channel blocker, for reduction of retinal injury associated with experimental glaucoma in rat and monkey.

    abstract::Glutamatergic excitotoxicity has been implicated as a mechanism for injury in a variety of central nervous system pathologies, including glaucoma. Memantine, an NMDA-type glutamatergic open-channel blocker, has pharmacologic properties that make its efficacy greater under excitotoxic conditions, but lesser under norma...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章


    authors: Hare W,WoldeMussie E,Lai R,Ton H,Ruiz G,Feldmann B,Wijono M,Chun T,Wheeler L

    更新日期:2001-05-01 00:00:00

  • Pediatric pseudotumor cerebri (idiopathic intracranial hypertension).

    abstract::This review focuses on the features of pseudotumor cerebri in the pediatric age group. There is no sex predilection in children, and obesity does not appear to be an important factor. Infants and young children may present with irritability, apathy, or somnolence, rather than headache. Dizziness and ataxia may also oc...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Lessell S

    更新日期:1992-11-01 00:00:00

  • Oculogenital disease.

    abstract::This review comprises brief discussions of the epidemiology, clinical manifestations, laboratory features, and treatment of the oculogenital diseases as they appear typically in the newborn infant, the child, and the adult. Inclusion conjunctivitis is the most frequently occurring oculogenital infection in all three c...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Ostler HB

    更新日期:1976-01-01 00:00:00

  • Retinoblastoma in adults. Report of three cases and review of the literature.

    abstract::Retinoblastoma usually presents in children younger than 5 years. To our knowledge, 20 cases of retinoblastoma in adults (older than 20 years) have been reported in the literature. Of 173 patients with histopathologically proven retinoblastoma seen in our institute, three were adults. All the patients had endophytic t...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Biswas J,Mani B,Shanmugam MP,Patwardhan D,Kumar KS,Badrinath SS

    更新日期:2000-03-01 00:00:00

  • Primary testicular and intraocular lymphomas: two case reports and a review of the literature.

    abstract::Testicular lymphoma is a rare neoplasm of the testis that is most commonly seen in older patients. It metastasizes preferentially to extranodal sites, including the skin, central nervous system, Waldeyer ring, contralateral testis, and lung. Two case reports of patients with a history of testicular lymphoma who develo...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章


    authors: Wallace DJ,Altemare CR,Shen DF,deSmet MD,Buggage RR,Nussenblatt RB,Chan CC

    更新日期:2006-01-01 00:00:00

  • Lymphoma of the eyelid.

    abstract::Lymphoma of the eyelid constitutes 5% of ocular adnexal lymphoma. In previously published cases, 56% of lymphomas of the eyelid are of B-cell origin and 44% are of T-cell origin. The most frequent B-cell lymphomas are extranodal marginal zone lymphoma (27 cases-14%) and diffuse large B-cell lymphoma (18 cases-9%). T-c...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Svendsen FH,Heegaard S

    更新日期:2017-01-01 00:00:00

  • Dermatologic diagnosis and treatment of itchy red eyelids.

    abstract::Distinguishing the cause of itching, red eyelids is often difficult. Pruritic, inflamed eyelids can reflect various etiologies and are a common clinical presentation to the office of a dermatologist or ophthalmologist. In this article, five of the more common causes of eyelid dermatitis (atopic dermatitis, contact der...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Zug KA,Palay DA,Rock B

    更新日期:1996-01-01 00:00:00

  • Systematic review and meta-analysis on the efficacy of selective laser trabeculoplasty in open-angle glaucoma.

    abstract::Selective laser trabeculoplasty (SLT) is a relatively new type of laser used in treating open-angle glaucoma (OAG) and is reported to be equally efficacious to a first-line medication and argon laser trabeculoplasty (ALT). We summarize available evidence for considering SLT as an alternative treatment in OAG through s...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,meta分析,评审


    authors: Wong MO,Lee JW,Choy BN,Chan JC,Lai JS

    更新日期:2015-01-01 00:00:00

  • Anterior chamber angle assessment techniques.

    abstract::Angle-closure glaucoma is a leading cause of irreversible blindness. Diagnosis and treatment are intricately related to angle assessment techniques. This article reviews the literature on angle assessment and provides recommendations about optimal techniques based on the published evidence. Specifically, we review gon...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Friedman DS,He M

    更新日期:2008-05-01 00:00:00

  • Central serous retinopathy: variants.

    abstract::Idiopathic central serous chorioretinopathy (ICSC) is a localized detachment of the sensory retina in the macula which is commonly seen by the general ophthalmologist. However, ICSC with bullous retinal detachment is a rare disease characterized by extensive sensory retinal detachment of the posterior pole and elsewhe...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章


    authors: Mazzuca DE,Benson WE

    更新日期:1986-11-01 00:00:00

  • As the Worm Turns: An Infant with Exotropia.

    abstract::A two-year-old full-term boy with a history of an intermittent exotropia presented to the pediatric ophthalmology clinic for routine follow-up. He was found to have a stable sensorimotor examination however dilated funduscopic examination of the right eye was significant for a dim foveal reflex with a new discrete, wh...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Hennein L,de Alba Campomanes AG,Afshar AR,Robbins SL

    更新日期:2020-12-10 00:00:00

  • Presbyopia toward the end of the 20th century.

    abstract::Recent advances in our understanding of the anatomy and physiology of accommodation have contributed to current concepts of the possible constituent factors in presbyopia, largely supplanting the long-held belief that presbyopia is due to sclerosis of the crystalline lens. In this review, the author examines epidemiol...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Weale R

    更新日期:1989-07-01 00:00:00

  • Posterior scleritis.

    abstract::Posterior scleritis must be considered in the differential diagnosis of many ocular conditions, including angle closure glaucoma, choroidal folds, optic disk edema, circumscribed fundus mass, choroidal detachment, and exudative retinal detachment. Because it is rare, a high index of suspicion is necessary. Anterior sc...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Benson WE

    更新日期:1988-03-01 00:00:00

  • A pituitary adenoma with dilated ventricles.

    abstract::It is generally accepted that postoperatively radiographic examinations be limited to the area of pathology. The patient presented here complained of visual difficulties two years after transphenoidal removal of a pituitary prolactinoma. His endocrinologist ordered what he believed to be the appropriate computed tomog...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章


    authors: Mindel JS,Fetell MR

    更新日期:1985-07-01 00:00:00

  • Clinical ophthalmology instruction for medical students.

    abstract::A ten-day ophthalmology clerkship for third-year medical students is described. The curriculum, detailed in a day-by-day syllabus, sets specific student goals, evaluates attainment of the goals by a pre-post test, utilizes a student-oriented study guide keyed to major textbrooks as core material with slide-tape and vi...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章


    authors: Waring GO 3rd,Harris R,Walters RF,Berris C

    更新日期:1977-09-01 00:00:00

  • Seeing through thick and through thin: Retinal manifestations of thrombophilic and hyperviscosity syndromes.

    abstract::The presence of retinal vasculopathy in the absence of typical predisposing factors should suggest a possible underlying hematologic abnormality. In such cases, a systemic investigation may reveal a potentially fatal hypercoagulability or hyperviscosity syndrome. Retinal vein occlusion is the most commonly encountered...

    journal_title:Survey of ophthalmology

    pub_type: 杂志文章,评审


    authors: Rajagopal R,Apte RS

    更新日期:2016-03-01 00:00:00