Abstract:
:Recombinant gibbon interleukin-3 (IL-3) is a multilineage hematopoietic colony-stimulating factor (CSF) that recently was cloned and found to be highly homologous with human IL-3. Gibbon IL-3, as well as human granulocyte-CSF (G-CSF) and human granulocyte-macrophage CSF (GM-CSF), stimulated normal human bone marrow cells to form myeloid colonies in soft agar in a sigmoidal dose-response manner. When IL-3 was added to increasing concentrations of G-CSF or GM-CSF, synergistic colony formation occurred as compared with the effects of each CSF alone. Synergism was also noted when G-CSF was added with GM-CSF and when all the CSFs were added simultaneously. The combination of IL-3 and GM-CSF was less stimulatory than all the other CSF combinations. At day 11 of culture, IL-3 induced granulocyte-macrophage (38%), eosinophil (30%), granulocyte (18%), and macrophage (14%) colony formation. In summary, gibbon IL-3 is a growth factor that can synergize with other CSFs to enhance proliferation of myeloid-committed progenitors, suggesting that combinations of CSFs may have clinical utility in patients with neutropenia of various etiologies.
journal_name
Bloodjournal_title
Bloodauthors
Paquette RL,Zhou JY,Yang YC,Clark SC,Koeffler HPsubject
Has Abstractpub_date
1988-06-01 00:00:00pages
1596-600issue
6eissn
0006-4971issn
1528-0020journal_volume
71pub_type
杂志文章相关文献
BLOOD文献大全abstract::Recently, the term monoclonal gammopathy of renal significance (MGRS) was introduced to distinguish monoclonal gammopathies that result in the development of kidney disease from those that are benign. By definition, patients with MGRS have B-cell clones that do not meet the definition of multiple myeloma or lymphoma. ...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2013-05-495929
更新日期:2013-11-21 00:00:00
abstract::Despite increasing use of targeted therapies to treat cancer, anemia remains a common complication of cancer therapy. Physician concerns about the safety of intravenous (IV) iron products and erythropoiesis-stimulating agents (ESAs) have resulted in many patients with cancer receiving no or suboptimal anemia therapy. ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019004017
更新日期:2020-08-13 00:00:00
abstract::Multidrug resistance (MDR) is often characterized by the expression of P-glycoprotein (P-gp), a 170-kd ATP-dependent drug efflux protein. As well as effluxing xenotoxins, functional P-gp can confer resistance to caspase-dependent apoptosis induced by a range of different stimuli, including Fas ligand, tumor necrosis f...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::Weibel-Palade bodies (WPB) are unique secretory organelles of endothelial cells that store factors regulating vascular hemostasis and local inflammation. Endothelial activation triggers rapid exocytosis of WPB, leading to the surface presentation of adhesion molecules relevant for leukocyte rolling (P-selectin) and pl...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019000786
更新日期:2019-09-19 00:00:00
abstract::Tissue inhibitor of metalloproteinases-1 (TIMP-1), the major physiological matrix metalloproteinase inhibitor and a potent antimetastatic factor, also stimulates the growth of erythroid progenitors (erythroid-potentiating activity). We analyzed the relationship between the growth factor activity and protease inhibitio...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-12-15 00:00:00
abstract::An acquired gain-of-function mutation in the Janus kinase 2 (JAK2-V617F) is frequently found in patients with myeloproliferative disorders (MPDs). To test the hypothesis that JAK2-V617F is the disease-initiating mutation, we examined whether all cells of clonal origin carry the JAK2-V617F mutation. Using allele-specif...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2005-11-009605
更新日期:2006-08-15 00:00:00
abstract::Chronic infection with Schistosoma mansoni induces in humans and mice a Th2-dominant immune response in which eosinophils and IgE are conspicuously elevated. Human eosinophils express IgE receptors that participate in an IgE-dependent eosinophil-mediated ADCC reaction against Schistosomula larvae in vitro. To investig...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-05-15 00:00:00
abstract::Mutations in a recently identified gene HJV (also called HFE2, or repulsive guidance molecule C, RgmC) are the major cause of juvenile hemochromatosis (JH). The protein product of HJV, hemojuvelin, contains a C-terminal glycosylphosphatidylinositol anchor, suggesting that it can be present in either a soluble or a cel...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-05-1845
更新日期:2005-10-15 00:00:00
abstract::We have previously shown that coculture of human embryonic stem cells (hESCs) for 14 days with immortalized fetal hepatocytes yields CD34(+) cells that can be expanded in serum-free liquid culture into large numbers of megaloblastic nucleated erythroblasts resembling yolk sac-derived cells. We show here that these pri...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-07-102087
更新日期:2008-02-15 00:00:00
abstract::Purified human lactoferrin was assessed for its influence in vivo in untreated mice and in mice undergoing rebound myelopoiesis after sublethal dosages of Cytoxan. Fully iron-saturated lactoferrin suppressed the numbers of granulocytes and monocytes per femur, the numbers of granulocyte-macrophage progenitor cells (CF...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1983-05-01 00:00:00
abstract::Hemoglobin and ferritin iron content have been followed during differentiation in tissue cultures of murine erythroleukemia cells (MELC) using the techniques of Mössbauer spectroscopy and electron microscopy. In undifferentiated cells grown without DMSO, only iron stored in ferritin was detected. The amount of iron in...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::The granulocyte-macrophage colony-stimulating factor receptor (GM-CSFR) is a potential target for toxin-directed therapy, because it is overexpressed on many leukemias and solid tumors and apparently not on stem cells. To investigate the potential therapeutic use of GM-CSF toxins, we fused human GM-CSF to truncated fo...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::It was recently shown that bacterial thymus-independent (TI) antigens confer long-lasting immunity and generate memory B lymphocytes. However, reactivation of TI memory B cells is repressed in immunocompetent mice, thus raising the issue of the mechanism whereby TI vaccines confer immune protection. Here, we propose a...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-01-200014
更新日期:2009-11-12 00:00:00
abstract::The myeloid cell-surface glycoprotein CD13/aminopeptidase N (APN; EC 3.4.11.2) contains a pentapeptide (HExxH) in its extracellular domain that is characteristic of many zinc-dependent metalloproteinases. This region contains residues important for zinc binding and constitutes part of the catalytic domain of several m...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-06-15 00:00:00
abstract::p38 MAP kinase in human platelets is activated by platelet agonists including thrombin, thromboxane A2 (TxA2), ADP, and others. However, both upstream mechanisms of p38 MAP kinase activation, and their downstream sequelae, are presently controversial and essentially unclear. Certain studies report sequential activatio...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-07-038158
更新日期:2007-01-15 00:00:00
abstract::Cytosine-phosphorothioate-guanine oligodeoxynucleotides (CpG ODNs) are synthetic ODNs with unmethylated DNA sequences that mimic viral and bacterial DNA and protect against infectious agents and tumor challenge. We show that CpG ODNs markedly accelerated graft-versus-host disease (GVHD) lethality by Toll-like receptor...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-09-113670
更新日期:2008-10-15 00:00:00
abstract::Recent studies have established that during embryonic development, hematopoietic progenitors and stem cells are generated from hemogenic endothelium precursors through a process termed endothelial to hematopoietic transition (EHT). The transcription factor RUNX1 is essential for this process, but its main downstream e...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-10-386094
更新日期:2012-07-12 00:00:00
abstract::Hematide is an investigational pegylated synthetic peptide that stimulates erythropoiesis in animal models and is being developed for the treatment of anemia associated with chronic renal failure and cancer. This study evaluated the safety and pharmacodynamics of single, intravenous doses (0.025, 0.05, and 0.1 mg/kg) ...
journal_title:Blood
pub_type: 杂志文章,随机对照试验
doi:10.1182/blood-2006-04-015818
更新日期:2006-09-15 00:00:00
abstract::Although allogeneic hematopoietic stem cell transplantation (HSCT) remains the only curative treatment for patients with Fanconi anemia (FA), published series mostly refer to single-center experience with limited numbers of patients. We analyzed results in 795 patients with FA who underwent first HSCT between May 1972...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood-2013-01-479733
更新日期:2013-12-19 00:00:00
abstract::It has been shown previously that multilineage human hematopoiesis is maintained within human fetal bone marrow (BM) fragments implanted into severe combined immunodeficient (SCID) mice. We describe here an application of this animal model, the SCID-hu mouse, to the study of human myeloid leukemias. BM cells from 8 pa...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-10-15 00:00:00
abstract::The natural history of lymphoid infiltrates of the orbit and conjunctiva is poorly understood. To determine if immunohistologic features could predict clinical outcome, these features were evaluated in 61 patients with orbital and conjunctival lymphoid infiltrates, including 44 patients with lesions localized to one o...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::Chimeric antigen receptor (CAR) T cells have radically improved the treatment of B cell-derived malignancies by targeting CD19. The success has not yet expanded to treat acute myeloid leukemia (AML). We developed a Sequentially Tumor-Selected Antibody and Antigen Retrieval (STAR) system to rapidly isolate multiple nan...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019002779
更新日期:2020-03-05 00:00:00
abstract::The zinc finger transcription factor GATA-2 is highly expressed in immature hematopoietic cells and declines with blood cell maturation. To investigate its role in normal adult hematopoiesis, a bicistronic retroviral vector encoding GATA-2 and the green fluorescent protein (GFP) was used to maintain the high levels of...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-01-15 00:00:00
abstract::In a chromosome study in childhood T-cell leukemia/lymphoma, we found t(7;11)(q35;p13) in 2 patients, t(7;14) (q35;q11) in one patient, and t(7;14)(p15;q32) in 1 patient. Southern blotting and in situ chromosomal hybridization studies in one patient with the t(7;11) demonstrated that both alleles of the T-cell antigen...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::Primary cutaneous lymphomas (PCLs) are clonal T- or B-cell neoplasms, which originate in the skin. In recent years, mast cells were described as regulators of the tumor microenvironment in different human malignancies. Here, we investigated the role of mast cells in the tumor microenvironment of PCL. We found signific...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-03-415638
更新日期:2012-09-06 00:00:00
abstract::A mutant strain of Escherichia coli (E. coli ML-35) was used to follow the kinetics of phagocytosis, perforation of the bacterial cell envelope, and inactivation of bacterial proteins by human neutrophils. This particular E. coli mutant strain has no lactose permease, but constitutively forms the cytoplasmic enzyme be...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-09-01 00:00:00
abstract::Caveolin-1 (Cav-1) is a key organizer of membrane specializations and a scaffold protein that regulates signaling in multiple cell types. We found increased Cav-1 expression in human and murine T cells after allogeneic hematopoietic cell transplantation. Indeed, Cav-1(-/-)donor T cells caused less severe acute graft-v...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-09-672428
更新日期:2016-04-14 00:00:00
abstract::In eighty-three patients with confirmed deep vein thrombosis, the fibrinolytic system was studied before and after a 10-minute venous occlusion. Blood was collected at least 3 months after the last acute episode, and PAI-1 antigen and activity, as well as tissue-type plasminogen activator (t-PA) antigen, urokinase-typ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::The effect of lipopolysaccharide (LPS) on endothelial cells is a key component of the inflammatory response seen in Gram-negative sepsis. LPS does not cause death of cultured human endothelial cells. However, when the expression of new proteins is inhibited by cycloheximide, microvascular endothelial cells in culture ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-10-15 00:00:00
abstract::The occurrence of childhood acute lymphoblastic leukemia (ALL) in 2 of 3 triplets provided a unique opportunity for the investigation of leukemogenesis and the natural history of ALL. The 2 leukemic triplets were monozygotic twins and shared an identical, acquired TEL-AML1 genomic fusion sequence indicative of a singl...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.2.478
更新日期:2001-07-15 00:00:00