Abstract:
:The expression of iron regulated proteins (IRPs) in vitro has been obtained in the past by adding iron chelators to the culture after bacterial growth, in the presence of an organic iron source. We have investigated aspects concerning full expression of the meningococcal IRPs during normal growth, in defined conditions using Catlin medium, Mueller Hinton and Tryptic Soy Broth (TSB). The expression of IRPs varied between different strains with respect to Ethylenediamine Di-ortho-Hidroxy-phenyl-acetic acid (EDDA) concentrations, and according to culture medium, and also between different lots of TSB. For each strain, a specific set of IRPs were expressed and higher EDDA concentrations, or addition of glucose, or use of different culture media did not resulted in a differential expression of IRPs. We were not able to grow N. meningitidis under normal growth conditions using Desferal. We looked for a good yield of outer membrane vesicles (OMVs) expressing IRPs in iron-deficient Catlin medium containing EDDA and Hemin. Culture for 32 h at 30 degrees C after growing for 16 h at 37 degrees C supported good bacterial growth. Bacterial lysis was noted after additional 24 h at 30 degrees C. Approximately 4 times more OMVs was recoverable from a culture supernatant after 24 h at 30 degrees C than from the cells after 16 h at 37 degrees C. The IRP were as well expressed in OMVs from culture supernatant obtained after 24 h at 30 degrees C as from the cells after 16 h at 37 degrees C.
journal_name
Rev Inst Med Trop Sao Paulojournal_title
Revista do Instituto de Medicina Tropical de Sao Pauloauthors
Brandileone MC,Zanella RC,Vieira VS,Sacchi CT,Milagres LG,Frasch CEdoi
10.1590/s0036-46651994000400002subject
Has Abstractpub_date
1994-07-01 00:00:00pages
301-10issue
4eissn
0036-4665issn
1678-9946journal_volume
36pub_type
杂志文章abstract::Lutzomyia verrucarum (Townsend, 1913) (Diptera: Psychodidae); the natural vector of Bartonella bacilliformis, agent of human bartonellosis (peruvian verruga or Carrion's disease), is a native species of Peru; its geographic distribution occurrs between latitudes 5 degrees and 13 degrees 25' South: in the Occidental an...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651993000600002
更新日期:1993-11-01 00:00:00
abstract::Recent human herpesvirus 6 (HHV-6) infection was detected in cases of exanthem subitum (ES) involving four children, aged 10 to 24 months, between April and August 1994, in Belém, Brazil. By using the indirect immunofluorescence antibody assay (IFA), significant increases (at least eight times) in antibody concentrati...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651995000600003
更新日期:1995-11-01 00:00:00
abstract::Cryo-microtome sections of larvae of Strongyloides stercoralis and S. ratti respectively obtained from human and rat feces cultures, were used as antigens. Fluoresceinate conjugates against human IgG were employed at the ideal titer of 10 for S. stercoralis and 100 for S. ratti. The sensitivity of the indirect immunof...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651997000600001
更新日期:1997-11-01 00:00:00
abstract::Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals. ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651996000500009
更新日期:1996-09-01 00:00:00
abstract::The circulation of arboviruses throughout the world and the maintenance of lymphatic filariasis endemicity in tropical countries, combined with the lack of vaccines and specific treatments, highlight the importance of reducing the populations of mosquitoes involved in the transmission of these pathogens, Aedes aegypti...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S1678-9946202062094
更新日期:2020-11-27 00:00:00
abstract::The aim of this study was to compare the fecundity and fertility of B. glabrata and B. straminea by cross- and self-fertilization. To attain this objective, laboratory-raised strains of B. glabrata and B. straminea were used. The former originated from natural breeding grounds in the municipality Paulista, state of Pe...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652004000300007
更新日期:2004-05-01 00:00:00
abstract::In order to contribute to a better understanding of the forms of acquisition of hepatitis C virus (HCV) in Brazil, with special emphasis on sexual transmission, we determined the presence of HCV infection in regular partners and in non-sexual home communicants of blood donors seen at Fundação Pró-Sangue Hemocentro de ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652001000300003
更新日期:2001-05-01 00:00:00
abstract::Pleural effusions are rare events accompanying histoplasmosis, and in general they are associated with acute forms of the disease. It is described here a case of histoplasmoma associated with pleuritic pain and pleural effusion. ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651990000500012
更新日期:1990-09-01 00:00:00
abstract::The aim of this study was to compare the frequency of headache between Chagasic and Non-chagasic women. The cross-sectional study comprised 647 female > or = 40 years old, Chagasic (n = 362) and Controls (n = 285) at a Brazilian University Hospital. Chagasic were classified as Cardiac (n = 179), Megas (n = 58) or Inde...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651999000200010
更新日期:1999-03-01 00:00:00
abstract::Sixteen patients with endemic pemphigus foliaceus were submitted to nutritional evaluation. Ten had the localized form of the disease (Group G1) and six the disseminated form (Group G2). The patients were submitted to anthropometric measurements (weight, height, Quetelét index, tricipital skin fold, subscapular skin f...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651995000100008
更新日期:1995-01-01 00:00:00
abstract::The objective of this study was to evaluate the larvicidal activity of diterpenoids obtained from the oil-resin of Copaifera reticulata against Aedes aegypti larvae, the principal vector of dengue and urban yellow fever. Four diterpenes were obtained from oil-resin extraction with organic solvents and subsequent chrom...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652008000100006
更新日期:2008-01-01 00:00:00
abstract::A sedimentation technique is described, in which a fecal suspension is placed on top of an aqueous sucrose solution of specific gravity 1.015 g/cm3. Using 100 by 15 mm test tubes, duplicate gravity sedimentation experiments were made using homogenized fecal suspensions (single-columns) and fecal suspensions placed on ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651992000300013
更新日期:1992-05-01 00:00:00
abstract::Cytomegalovirus (CMV) infection is the most common congenital infection, affecting 0.4% to 2.3% newborns. Most of them are asymptomatic at birth, but later 10% develop handicaps, mainly neurological disturbances. Our aim was to determine the prevalence of CMV shed in urine of newborns from a neonatal intensive care un...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652000000300003
更新日期:2000-05-01 00:00:00
abstract::A proven case of human infection caused by Angiostrongylus costaricensis is reported for the first time in Venezuela. The patient was a 57-year-old female surgically operated because of signs of peritonitis with a palpable mass at the lower right quadrant of the abdomen. WBC count reported 16,600 cells/mm3, with 46% e...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章,评审
doi:10.1590/s0036-46652007000300012
更新日期:2007-05-01 00:00:00
abstract::In the São Paulo State, Brazil, where the Biomphalaria tenagophila is the intermediate host, the Ribeira Valley is an important endemic schistosomiasis mansoni area. During last eleven years there has been intense control measures focusing on schistosomiasis. The efforts have been concentrated in the municipalities of...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651992000300003
更新日期:1992-05-01 00:00:00
abstract::Rotavirus and pathogenic free-living amoebae are causative agents of important health problems, especially for developing countries like Pakistan where the population has limited access to clean water supplies. Here, we evaluated the prevalence of rotavirus and free-living amoebae (Acanthamoeba spp., Balamuthia mandri...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S1678-9946201759032
更新日期:2017-06-01 00:00:00
abstract::Human T-lymphotropic virus type 1 (HTLV-1) is found in indigenous peoples of the Pacific Islands and the Americas, whereas type 2 (HTLV-2) is widely distributed among the indigenous peoples of the Americas, where it appears to be more prevalent than HTLV-1, and in some tribes of Central Africa. HTLV-2 is considered an...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章,评审
doi:10.1590/S0036-46652015000100001
更新日期:2015-01-01 00:00:00
abstract::Formalin-killed promastigotes (FKP) of Leishmania major, in combination with Montanide ISA 720 (MISA), BCG or alum were used in vaccination of an inbred murine model against cutaneous leishmaniasis (CL). Significant and specific increases in anti-FKP IgG responses were detected for both alum-FKP and BCG-FKP compared t...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652010000200006
更新日期:2010-03-01 00:00:00
abstract::In the study of conjunctivitis outbreaks occurring from September 1994 to September 1996 in the region of Ribeirão Preto, conjunctival exudates of 92 patients were cultivated in Instituto Adolfo Lutz Laboratory I, Ribeirão Preto. Most cases occurred in the age range 2-7 years. The etiological agents which were most fr...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651998000100002
更新日期:1998-01-01 00:00:00
abstract::Experiments were carried out to test the susceptibility of Biomphalaria tenagophila to the infection with strain SJ of Schistosoma mansoni in the F1, F2 and non-selected parental generation. The potential adaptation of B. tenagophila to desiccation, in healthy mollusks and those exposed to the larvae of S. mansoni of ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652002000400003
更新日期:2002-07-01 00:00:00
abstract::In the last years, new techniques of neuroimages and histopathological methods have been added to the management of cerebral mass lesions in patients with AIDS. Stereotactic biopsy is necessary when after 14 days of empirical treatment for Toxoplasma gondii encephalitis there is no clinical or neuroradiologic improvem...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章,评审
doi:10.1590/s0036-46652004000400004
更新日期:2004-07-01 00:00:00
abstract::This study aims to assess the association between schistosomiasis and hookworm infection with hemoglobin levels of schoolchildren in northern Mozambique. Through a cross-sectional survey, 1,015 children from five to 12 years old in the provinces of Nampula, Cabo Delgado and Niassa were studied. Hookworm infection and ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652014000300007
更新日期:2014-05-01 00:00:00
abstract::Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol reagent had to be first purified to generate fragments of 170 pb and 6...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652006000100010
更新日期:2006-01-01 00:00:00
abstract::Candidemia remains a major cause of morbidity and mortality in the health care environment. The epidemiology of Candida infection is changing, mainly in relation to the number of episodes caused by species C. non-albicans. The overall objective of this study was to evaluate the frequency of yeasts of the genus Candida...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652014000400006
更新日期:2014-07-01 00:00:00
abstract::The formalin-Tween sedimentation method was compared with the formalin-ether sedimentation for parasitic detection. Of a total 297 fecal specimens examined, 72.1% were positive. The formalin-tween technique was effective for ascertaining helminths, particularly Ascaris lumbricoides, Trichuris trichiura and hookworm eg...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652003000500009
更新日期:2003-09-01 00:00:00
abstract::The vasoactive intestinal peptide (VIP) expression is lower in cardiac chagasic patients and is related to worse cardiac function. The reduction of VIP in patients with Chagas disease may be a result of its enhanced degradation. To test this hypothesis, the tryptase and chymase expression was evaluated. We also relate...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S1678-9946201860057
更新日期:2018-10-22 00:00:00
abstract::Thirty eight paralysis cases classified as Guillain-Barré syndrome (GBS) in Brazil were analysed. In all these cases Sabin-related poliovirus vaccine strains were isolated. In most of the cases the last vaccine dose was given months or years before the onset of GBS, suggesting a persistent infection or the transmissio...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651996000100010
更新日期:1996-01-01 00:00:00
abstract::We determined the relationship between plasma and red blood cell concentrations of quinine in children with uncomplicated falciparum malaria from an endemic area of Amazonian region. Quinine was determined by high performance liquid chromatography with ultraviolet detection. In the steady state the ratio between plasm...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 临床试验,杂志文章
doi:10.1590/s0036-46652009000200009
更新日期:2009-03-01 00:00:00
abstract::The prevalence of thermotolerant Campylobacter in diarrhoeic and healthy children as well as in dogs, hens, ducks and pigeons was determined in Southern Chile. Campylobacter were found in 34.5% of the faecal samples examined. The isolation rate of Campylobacter in diarrhoeic and healthy children was 16.3% and 6.4% res...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651994000500007
更新日期:1994-09-01 00:00:00
abstract::A total of 868 (84.89%) patients diagnosed with tetanus were studied, out of the 1,024 tetanus patients hospitalized at Couto Maia Hospital (Salvador, Bahia, Brazil), during the period between 1986 and 1997. Of this group (n = 868), 63.5% (n = 551) were discharged, 35.4% (n = 307) died, and 1.1% (n = 10) were transfer...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652003000100007
更新日期:2003-01-01 00:00:00