Abstract:
OBJECTIVES:MDM2 gene amplification is associated with well-differentiated (WDL) and dedifferentiated liposarcomas (DDL). Using fluorescent in situ hybridization (FISH), we sought to characterize various patterns of MDM2 amplification among the morphologic spectrum of liposarcoma. METHODS:Forty-six cases of liposarcoma in various sites were examined and included 22 WDLs, 14 DLLs, and 10 negative control subjects. RESULTS:The MDM2 amplification ratio (MDM2/CEP12) was lower in WDL (10.2) compared with DDL (18.3) cases (P = .0000002). An amplification ratio of 16 showed optimal sensitivity (0.86) and specificity (0.96) as a cutoff point for progression to DDL. Borderline areas, defined as tumors with increased cellularity and atypia but with preserved lipomatous differentiation, showed a significantly higher MDM2 ratio (17.5; P = .0007) compared with WDL. Central (retroperitoneal and intra-abdominal) tumors also showed a significantly higher MDM2 ratio than peripheral ones (P = .02). CONCLUSIONS:Differences in MDM2 amplification profiles among liposarcomas could help further define and predict progression to high-grade neoplasia.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Ware PL,Snow AN,Gvalani M,Pettenati MJ,Qasem SAdoi
10.1309/AJCPLYU89XHSNHQOsubject
Has Abstractpub_date
2014-03-01 00:00:00pages
334-41issue
3eissn
0002-9173issn
1943-7722pii
141/3/334journal_volume
141pub_type
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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doi:
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abstract::With various methods, BRAF mutations have been detected in 73.4% to 87.1% of papillary thyroid carcinomas (PTCs) in Korea. We assessed the ability of allele-specific polymerase chain reaction using a dual priming oligonucleotide system, compared with direct sequencing and pyrosequencing, to detect BRAF mutations in fi...
journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/82.5.526
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
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journal_title:American journal of clinical pathology
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更新日期:1994-02-01 00:00:00
abstract:OBJECTIVES:Timely reporting of immunosuppressant (ISP) drug level results is needed for transplant patient management. This study characterized the local ISP testing process, identified bottlenecks and implemented process improvements to meet turnaround time requirements. METHODS:Laboratory information time stamps, di...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw087
更新日期:2016-08-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPH2A4XTXJUKDZ
更新日期:2014-10-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1093/ajcp/96.3.341
更新日期:1991-09-01 00:00:00
abstract:OBJECTIVES:High-grade serous carcinoma (HGSC) is the most common ovarian malignancy. The role of cytopathology in obtaining tissue diagnosis before institution of neoadjuvant chemotherapy (NACT) was evaluated. METHODS:All histopathology-proven HGSC specimens between 2015 and 2018 with prior cytopathologic diagnosis by...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa028
更新日期:2020-06-08 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.6.749
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journal_title:American journal of clinical pathology
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doi:10.1309/BM40-YM6J-9T3X-MH8H
更新日期:2001-09-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP9HDXYB2WYYJX
更新日期:2013-07-01 00:00:00
abstract::The presence of antineutrophil cytoplasmic autoantibodies with specificity for proteinase 3 (PR3-ANCA) usually is detected by enzyme-linked immunosorbent assay (ELISA) with purified PR3 as a substrate. We studied the technical performance of direct and capture ELISA using a recombinant proteolytically inactive form of...
journal_title:American journal of clinical pathology
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doi:10.1309/YTU2-FUHB-EXJL-WMLD
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journal_title:American journal of clinical pathology
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abstract::Gc allele frequencies were determined in Haitian immigrants, a group at high risk for AIDS. Gc2, an allele associated with disease resistance, is infrequent in this group; Gc1f is frequent. ...
journal_title:American journal of clinical pathology
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doi:10.1093/ajcp/90.3.371
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journal_title:American journal of clinical pathology
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abstract::Estrogen receptor (ER), progesterone receptor (PR), and c-ERBB2 (HER2/neu) are therapeutically and prognostically important markers in the management of breast carcinoma. They are not always analyzed in distant metastatic and locally recurrent breast cancers. We compared immunohistochemical expression in a series of p...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPJ57FLLJRXKPV
更新日期:2010-03-01 00:00:00
abstract:Objectives:Fibromatosis of the breast is an uncommon neoplasm with potential for local recurrence. Treatment has traditionally been surgical excision with current trends toward conservative management. Given the option of observation after diagnosis by core needle biopsy (CNB), we sought to evaluate the accuracy of CNB...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqx065
更新日期:2017-09-01 00:00:00
abstract::Histologic follow-up of patients with the Bethesda system cervical-vaginal diagnosis of atypical glandular cells of undetermined significance (AGUS), "favor endocervical origin", or "not otherwise specified" (NOS) shows a high percentage of clinically significant (neoplastic or preneoplastic) lesions. Using the criter...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/107.3.299
更新日期:1997-03-01 00:00:00
abstract:OBJECTIVES:Method imprecision, error rates, and explanatory causes that were identified in the Institute for Quality Management in Healthcare point-of-care (POC) glucose proficiency testing (PT) program were assessed in comparison with results obtained from laboratory glucose PT surveys. METHODS:POC and laboratory glu...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPP5YS2MVSKBYY
更新日期:2014-12-01 00:00:00
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pub_type: 杂志文章
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abstract::Allergic adenoiditis is defined as the presence of numerous brightly fluorescent IgE mast cells (FIEMC) demonstrated in formalin-fixed adenoid tissue by the trypsin-immunofluorescent method. A prospective study of 606 routinely accessioned cases seen during a 39-month period revealed 98 positive cases. Correlation wit...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/81.2.170
更新日期:1984-02-01 00:00:00
abstract:OBJECTIVES:Blood component waste is an important issue at all hospitals. As an initiative of the patient blood management program at a regional health care system, the causes and extent of blood product wastage were identified, and targeted interventions to effect a reduction were implemented. METHODS:Multiple low-cos...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:2015-03-01 00:00:00